MOLECULAR CHARACTERIZATION OF Dunaliella salina AND Chlorella vulgaris FUSANT USING 18SrDNA GENE

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Hermin Pancasakti Kusumaningrum ◽  
Muhammad Zainuri
Keyword(s):  
Quantitative Analysis ◽  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Efficient Method ◽  
Dna Sequences ◽  
Dunaliella Salina ◽  
Parental Genome ◽  
Carotenoid Production ◽  
Relationship Of

Protoplast fusion was found to be an efficient method in improving carotenoid production from fusant of carotenogenic microalgae D. salina and C. vulgaris. Molecular characterization is needed for identifying the dominant parental genome in the fusant using ribosomal DNA sequences. The research was carried out by analyzing the gene encodes for 18S rDNA of fusant and determining relationship of fusant with D. salina and C. vulgaris species from GenBank. Quantitative analysis showed that C. vulgaris was not remarkably dominant in fusant with 84 % similarity compare to D. salina with 82 % similarity. The result indicated that the fusant gained both character from their parents.

Molecular characterization of Fasciola gigantica from Mauritania based on mitochondrial and nuclear ribosomal DNA sequences

2011 ◽  
Vol 129 (2) ◽  
pp. 127-136 ◽  
Author(s):  
Nabil Amor ◽  
Sarra Farjallah ◽  
Mohamed Salem ◽  
Dia Mamadou Lamine ◽  
Paolo Merella ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Fasciola Gigantica ◽  
Nuclear Ribosomal Dna

Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

2011 ◽  
Vol 128 (3) ◽  
pp. 196-204 ◽  
Author(s):  
Nabil Amor ◽  
Ali Halajian ◽  
Sarra Farjallah ◽  
Paolo Merella ◽  
Khaled Said ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Endemic Area ◽  
Nuclear Ribosomal Dna ◽  
Northern Iran ◽  
Fasciola Spp

Molecular characterization of small and medium segments of Crimean-Congo hemorrhagic fever virus in Turkey

2020 ◽  
Vol 15 (4) ◽  
pp. 247-254
Author(s):  
Murat Karamese ◽  
Erkan Ozmen ◽  
Hakan Aydin ◽  
Mehmet Ozkan Timurkan ◽  
Mesud Fakirullahoglu
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Relationship Of

Aim: The objective was to investigate the genotypic relationship of S and M segments in Crimean-Congo hemorrhagic fever virus (CCHFV) by phylogenetic analysis in 25 patients from seven endemic cities in Turkey. Materials & methods: A total of 25 samples from patients with CCHF were included between 2012 and 2015. Phylogenetic tree analyses were inferred using MEGA version-6.0 and distances were calculated by Kimura’s 2-parameter. Results: Phylogenetic analysis showed that all isolated viruses (n = 25) were in the predicted clades such as clade V- Europe-1 regarding both S and M segments of the CCHFV. Conclusion: Further epidemiological, molecular and phylogenic studies should be performed in both reservoir animals/vectors and humans to determine the incidence of tick-borne infectious disease and to help to develop vaccines for prevention of the disease.

Mapping and molecular characterization of five HMG1-related DNA sequences

1998 ◽  
Vol 83 (1-2) ◽  
pp. 124-129 ◽  
Author(s):  
P. Rogalla ◽  
Z. Borda ◽  
K. Meyer-Bolte ◽  
K.H. Tran ◽  
S. Hauke ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences

Evolutionary relationships ofHyphodiscus hymeniophilus(anamorphCatenulifera rhodogena) inferred from β-tubulin and nuclear ribosomal DNA sequences

2006 ◽  
Vol 84 (2) ◽  
pp. 243-253 ◽  
Author(s):  
Wendy A. Untereiner ◽  
Françoise A. Naveau ◽  
Jason Bachewich ◽  
Andrea Angus
Keyword(s):  
Phylogenetic Relationship ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Its Sequences ◽  
Nuclear Ribosomal Dna ◽  
Phialocephala Fortinii ◽  
Piptoporus Betulinus ◽  
Close Phylogenetic Relationship ◽  
Relationship Of ◽  
Β Tubulin

During an investigation of lignicolous ascomycetes from Luxembourg, we isolated a Phialophora -like species that produced striking red colonies. To confirm the identity of this fungus as Catenulifera rhodogena , we compared it with isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus on a variety of media. Portions of the β-tubulin gene and the nuclear ribosomal DNA cistron (internal transcribed spacers (ITS) and large subunit (LSU)) were sequenced to examine the relationship of isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus from different substrates and to test the hypothesis that Cadophora and Catenulifera are congeneric. The phylogenetic position of Catenulifera within the Ascomycota was investigated based on the analysis of the small-subunit (SSU) rDNA sequences. The isolates examined were indistinguishable micromorphologically and closely related phylogenetically. Three strains of Catenulifera rhodogena from bark or wood and one strain from Piptoporus betulinus formed a strongly supported clade in analyses of β-tubulin and ITS sequences. This clade did not encompass the ex-type isolates of Cistella rubescens and Scopulariopsis rhodogena or a second isolate of Catenulifera rhodogena from Piptoporus betulinus. Analysis of partial LSU sequences confirmed the close phylogenetic relationship of Catenulifera rhodogena and Hyphodiscus hymeniophilus but provided no evidence that the isolates could be grouped by substrate or that Catenulifera is synonymous with Cadophora. The position of Catenulifera within the Helotiales was not resolved based on the comparison of LSU and SSU sequences, but the isolate for which we obtained complete SSU sequence grouped with the root endophyte Phialocephala fortinii. Comparison of ITS sequences confirmed the close phylogenetic relationship of Hyphodiscus to members of the Dermateaceae and Hyaloscyphaceae.

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