Mixed course of Fasciolosis and Paramphistomatosis in sheep in Uzbekistan

The purpose of the research to study the mixed course of sheep infection, caused by pathogens of fasciolosis and paraphistomatosis, in the conditions of Samarkand and Tashkent regions. Materials and methods. The research material was fallen and forcibly killed sheep from two regions of Uzbekistan, simultaneously infected by Fasciola gigantica and Paramphistomum spp. (Gastrothylax crumenifer, Calicophoron calicophorum). The animals' liver was subjected to complete helminthological dissection, followed by the presence of young immature in the parenchyma, and in the bile ducts – sexually mature fasciola. To detect pathogens of paramphistomatosis, we examined the scar and mesh, and, if necessary, the mucous membranes of the abomasum and the initial part of the small intestine. To kill the collected F. gigantica and complete bleeding with a Paramphistomum spp., they were kept in water for one and three to four days, respectively. After they took their natural form, their type, age and number were determined, and then fixed in Barbagallo fluid. If necessary, coprological studies of sheep and malacological studies of biotopes of freshwater mollusks, intermediate hosts of the studied trematodes, were carried out. Results and discussion. In recent years, cases of a mixed course in sheep fasciolosis (F. gigantica) and paramphistomatosis in farms of Uzbekistan have become more frequent. When opening the liver of the fallen sheep, up to 278 specimens were found immature F. gigantica and up to 3994 sp. G. crumenifer. In some farms, the death of individual owners' sheep from calicophorosis caused by C. calicophorum was observed with an intensity of infection of 592 sp. of C. calicophorum.

Prevalence of Paramphistomosis in Domestic Ruminants in Chittoor District of Andhra Pradesh, India

A total of 1169 dung samples from domestic ruminants were examined to record the prevalence of paramphistomosis infection. In cattle, the prevalence of infection by direct smear (DS) and sedimentation method (SD) was 17.43% and 31.19%, respectively. In sheep, the prevalence rates were recorded higher than cattle (26.09% and 33.18%). In goats, the prevalence of paramphistomosis was 20.66% and 30.52%. Out of 109 cattle carcasses, 47 were found with flukes in rumen, reticulum and bile duct during slaughterhouse examination (SH). In sheep and goat, the prevalence rates were 42.15% and 40.85%, respectively by SH. Age-wise the highest prevalence was recorded in cattle of 2-4 years (50.0%) by SH. In goats, the prevalence of infection was lower in the age group oflessthan1 year, when compared to their counterparts in sheep. In contrast, the higher prevalence was noticed in >1-2 years old goats (63.27%) than sheep of the same age group (36.85%). Sex-wise, statistically no significant difference was observed between male and female animals. Morphologically identified, Cotylophoron cotylophorum, Paramphistomum cervi, Gastrothylax crumenifer, Fischoederius elongatus and Gigantocotyle spp. In conclusion, an overall prevalence of 24.29%, 32.51% and 42.0% of paramphistomosis infection was recorded in cattle, sheep and goats by DS, SD and SH examinations, respectively.