Antimicrobial Activity of Oils and Extracts of Cymbopogon citratus (Lemon Grass), Eucalyptus citriodora and Eucalyptus camaldulensis

2007 ◽  
Vol 7 (4) ◽  
pp. 694-697 ◽  
Author(s):  
B.C. Akin-Osana ◽  
A.S. Agbaji . ◽  
M.A. Dakare .
Keyword(s):  
Antimicrobial Activity ◽  
Eucalyptus Camaldulensis ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Lemon Grass

scholarly journals Antibacterial Efficacies of Extracts of Lemon Grass (Cymbopogon citratus) on Some Clinical Microbial Isolates

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Kamoldeen Ajijolakewu ◽  
Muinat Olanike Kazeem ◽  
Risikat Nike Ahmed ◽  
Rahmat Folashade Zakariyah ◽  
Tariq Oluwakunmi Agbabiaka ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Active Principle ◽  
Cymbopogon Citratus ◽  
Lemon Grass

The spread of new infections and the emergence of multidrug resistant strains of microorganisms necessitate the search for new antimicrobials with focus on plant extracts. In this study, the antimicrobial activity of the extracts (aqueous and ethanolic) of lemon grass known usually for bioactive essential oil (Cymbopogon citratus) was investigated in-vitro, against selected clinical isolates (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans). Antimicrobial efficacy of the extracts was assessed using the agar-well diffusion and broth-dilution techniques. The aqueous extract of C. citratus was more efficacious as it showed higher antimicrobial activity against all the test isolates at all tested concentrations. In contrast, only two of the test isolates; P. aeruginosa and S. aureus were susceptible to the ethanolic extract with minimum inhibitory (MIC) concentrations of 2 mg/ml (against S. aureus) and 12.5 mg/ml (against P. aeruginosa). The MIC of the aqueous extract were (mg/ml): 2, 6.25, 12.5, 25 and 50 against S. aureus, E. faecalis, P. aeruginosa, E. coli and C. albicans respectively. Meanwhile, both the aqueous and ethanolic extracts of C. citratus exerted only a bacteriostatic effect against the five test isolates at all concentrations. Quantitative phytochemical screening of the two extracts revealed the presence of more concentration of active compounds in the aqueous extract than the ethanolic extract which plausibly accounts for its greater antimicrobial activity. This study has shown that, in difference to the use of its conventional essential oil, solvent extraction of Cymbopogon citratus is also a potent source of efficacious antimicrobials. Further works to determine the respective mechanisms of action(s) of the extracts’ active principle is being projected. Keywords: Lemon grass, clinical isolates, antimicrobial efficacies, bioactive compounds

scholarly journals Evaluation of In Vitro Activity of Essential Oils againstTrypanosoma brucei bruceiandTrypanosoma evansi

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Nathan Habila ◽  
Abel S. Agbaji ◽  
Zakari Ladan ◽  
Isaac A. Bello ◽  
Emmanuel Haruna ◽  
...  
Keyword(s):  
Essential Oils ◽  
Eucalyptus Camaldulensis ◽  
Trypanosoma Evansi ◽  
In Vitro Activity ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Trypanosoma Brucei Brucei ◽  
Short Period ◽  
Dose Dependent

Essential oils (EOs) fromCymbopogon citratus(CC),Eucalyptus citriodora(EC),Eucalyptus camaldulensis(ED), andCitrus sinensis(CS) were obtained by hydrodistillation process. The EOs were evaluated in vitro for activity againstTrypanosoma brucei brucei(Tbb) andTrypanosoma evansi(T. evansi). The EOs were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. The drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/mL, and 0.1 g/mL for all the EOs. The concentration of 0.4 g/mL CC was more potent at 3 minutes and 2 minutes for Tbb andT. evansi, respectively. The GC-MS analysis of the EOs revealed presence of Cyclobutane (96.09%) in CS, 6-octenal (77.11%) in EC, Eucalyptol (75%) in ED, and Citral (38.32%) in CC among several other organic compounds. The results are discussed in relation to trypanosome chemotherapy.

scholarly journals The Mosquito Larvicidal Activity of Essential Oils from Cymbopogon and Eucalyptus Species in Vietnam

Insects ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 128 ◽  
Author(s):  
Ho Dung Manh ◽  
Do Thi Hue ◽  
Nguyen Thi Thanh Hieu ◽  
Doan Thi Thanh Tuyen ◽  
Ong Thi Tuyet
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Eucalyptus Camaldulensis ◽  
Probit Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Eucalyptus Species ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Cymbopogon Winterianus

The larvicidal activity of essential oils (EOs) extracted from Cymbopogon citratus, Cymbopogon winterianus, Eucalyptus citriodora, and Eucalyptus camaldulensis aromatic plants grown in Vietnam was evaluated on Aedes aegypti larvae. The EOs were hydro-distilled in a Clevenger-type apparatus. The EOs were analyzed by gas chromatography–mass spectrometry (GC–MS). The mortality rates obtained from the bioassays were used to calculate the lethal concentrations (LC50) of the EOs by the probit analysis method. These essential oils exhibited toxicity to the larvae of Aedes aegypti. Results were obtained for Cymbopogon citratus (LC50 = 120.6 ppm), Cymbopogon winterianus (LC50 = 38.8 ppm), Eucalyptus citriodora (LC50 = 104.4 ppm), and Eucalyptus camaldulensis (LC50 = 33.7 ppm). The essential oils of Eucalyptus camaldulensis and Cymbopogon winterianus were found to be the most efficient, and their respective values of LC50 were 33.7 ppm, 38.8 ppm. In conclusion, this research adds to the growing body of literature on natural larvicides from essential oils against Aedes aegypti mosquitoes.

scholarly journals Antimicrobial Activity of Cymbopogon Citratus (Lemon Grass) and It’s Phytochemical Properties

2013 ◽  
Vol 2 (6) ◽  
pp. 214-220 ◽  
Author(s):  
Ewansiha J. U. ◽  
Garba S. A. ◽  
Mawak J. D. ◽  
Oyewole O. A.
Keyword(s):  
Antimicrobial Activity ◽  
Cymbopogon Citratus ◽  
Lemon Grass

scholarly journals In vitro antimicrobial activity of essential oil of Cymbopogon citratus (lemon grass) on Streptococcus mutans biofilm

2016 ◽  
Vol 10 (31) ◽  
pp. 1224-1228 ◽  
Author(s):  
Bringel Rego Camilla ◽  
Menezes Silva Andreia ◽  
Machado Gon ccedil alves Let iacute cia ◽  
Aurelio Benini Paschoal Marco
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Cymbopogon Citratus ◽  
In Vitro Antimicrobial Activity ◽  
Lemon Grass

scholarly journals Comparative Antimicrobial Activity of Lemon Grass (Cymbopogon citratus) and Garlic (Allium sativum) Extracts on Salmonella typhi

2019 ◽  
pp. 1-9
Author(s):  
O. F. Olukunle ◽  
O. J. Adenola
Keyword(s):  
Antimicrobial Activity ◽  
Clinical Isolate ◽  
Allium Sativum ◽  
Water Extract ◽  
Antimicrobial Effect ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Cymbopogon Citratus ◽  
Lemon Grass ◽  
Zones Of Inhibition

In the genesis of using plants for treatment of diseases, Garlic and lemon grass were some of the earliest plants that have proven effective. In this study the antimicrobial effect of Cymbopogon citratus (lemon grass) and Allium sativum (garlic) extracts on clinical isolate of Salmonella typhi  was examined. Water, ethanol and methanol were used as extraction solvent. Antimicrobial effects of conventional antibiotics was assayed using disc diffusion method while antimicrobial activity of lemon grass and garlic extracts were carried out using agar well diffusion method and the zones of inhibition was measured in millimeter (mm). Comparatively, the highest percentage yield of extract was observed in water extract of garlic (21.83%) and lemon grass (6.67%). Ciprofloxacin and Perfloxacin have the highest zones of inhibition of 19.73±0.24mm and 14.47±0.31mm respectively against the clinical isolates of Salmonella typhi . Two out of the twelve clinical isolate of Salmonella typhi  used were multi drug resistant. Lemon grass extracts did not show any antimicrobial effects on the clinical isolates of Salmonella typhi  while garlic extracts showed antimicrobial effect at the concentration of 800 mg/ml, water extract of garlic has the highest zone of inhibition (14.00 mm) against the tested isolates. Therefore garlic extract could be used for the formulation of drug and treatment of Salmonella typhi   infection.

scholarly journals Cytogenotoxicity of Cymbopogon citratus (DC) Stapf (lemon grass) aqueous extracts in vegetal test systems

2010 ◽  
Vol 82 (2) ◽  
pp. 305-311 ◽  
Author(s):  
Saulo M. Sousa ◽  
Pâmela S. Silva ◽  
Lyderson F. Viccini
Keyword(s):  
Root Development ◽  
Folk Medicine ◽  
Root Tip ◽  
Aqueous Extracts ◽  
Cymbopogon Citratus ◽  
Important Species ◽  
Cellular Behavior ◽  
Lettuce Seeds ◽  
Lemon Grass ◽  
Poaceae Family

The lemon grass, Cymbopogon citratus (DC) Stapf, is an important species of Poaceae family commonly used in the folk medicine in many countries. The aim of this study was to investigate the cytotoxic and genotoxic effects of aqueous extracts from C. citratus leaves on Lactuca sativa (lettuce) root tip meristem cells by cytogenetic studies that have never been done before for lemon grass extracts. For this, lettuce seeds were treated for 72h with different concentrations of lemon grass aqueous extracts (5; 10; 20 and 30 mg/mL). The percentage of germination, root development and cellular behavior were analyzed, and the results showed that the highest concentration of aqueous extracts reduced the mitotic index, the seed germination and the root development of lettuce. The extracts have also induced chromosome aberrations and cellular death in the roots cells of L. sativa.

scholarly journals Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

2018 ◽  
Vol 66 (4) ◽  
pp. 1519
Author(s):  
Mailen Ortega Cuadros ◽  
Adriana Patricia Tofiño Rivera ◽  
Luciano Jose Merini ◽  
Maria Cecilia Martinez Pabon
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Human Fibroblasts ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

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