The effect of fed on feeding combination of the ark with ammonized citronella grass waste to the quality of lactated etawah crossbreed goat milk

This study aims to determine the effect of giving a combination of the ark with ammoniated citronella grass waste on the levels of protein, fat, lactose and density of lactated Etawah crossbreed goat milk. The design used in this study was a randomized block design (RBD) consisting of 5 treatments and 3 groups. The treatment consisted of P1 (0% ark: 8% ammoniated citronella grass waste), P2 (2% ark: 6% ammoniated citronella grass waste), P3 4% ark: 4% ammoniated citronella grass waste), P4 (6% ark: 2% citronella ammonia grass waste) and P5 (8% ark: 0% ammoniated citronella grass waste). The data obtained were analysed statistically using Microsoft Excel software. Based on the results of the research, giving the combination of the ark with ammoniated citronella grass waste shows no significant effect (P> 0.05) on the quality of milk which includes density, lactose level, protein level and fat level in Etawah crossbreed goat (PE) milk. However, the quality test results showed an increasing trend when compared with the quality of PE goat’s milk without treatment The results of the data for each of the PE goat’s milk quality before given the feed treatment were 1.027; 3.34%; 3.42% and 6.40% for the density, lactose level, protein level and fat level. Meanwhile the results of the data for each of the PE goat milk quality after given the feed treatment got the best results, namely 1.030; 3.66%; 3.78% and 6.55% for the density, lactose level, protein level and fat level.

Analysis of the chemical composition, antifungal activity and larvicidal action against Aedes aegypti larvae of the Essential Oil Cymbopogon nardus

The Cymbopogon nardus L. is a plant popularly known as "citronella grass", originating from Ceylon and India, used in Indonesia as a soothing and digestive tea. The essential oil of the species Cymbopogon nardus (OECN) is used in the manufacture of cosmetics and perfumes, besides having shown antimicrobial action against Escherichia coli, Salmonella spp., Pseudomonas spp., Streptococcus spp., and in addition antioxidant, anti-inflammatory. The objective of this study was to determine the larvicidal and fungicide potential of OECN extracted from the fresh leaves of C. nardus. L at the beginning of the dry season in Teresina, PI. From the OECN extracted by hydrodistillation, the actives were identified by mass gas chromatography. The larvicidal action of OECN was tested against the third and fourth larval stages of Aedes aegypti at concentrations (2.5, 5.0, 7.5 and 10 μL/20 ml) for 24 to 48 hours. Antifungal activity for Aspergillus flavus and A. parasiticus at concentrations (1.0, 2.0, 4.0 6.0 and 8.0 μL/10 mL). The OECN showed a good yield (1.0%), with 26 assets (93.2%) identified with the majority: citronelal (31.6%), geraniol (22.1%), elemol (11.8%) and citronellol (8.2%). Mortality of 100% of the larvae was observed at concentrations of 7.5 and 10.0 µl / 20 ml in 24 hours. After 48 hours 93.3% in 5.0 µl/10 ml OECN and 70% in 2.5 µl/10 ml. A. flavus showed greater sensitivity to OECN at 8.0 µl / 10 ml than A. parasiticus at concentrations. A. parasiticus was the most sensitive at concentrations of 1.0 and 2.0 µL / 10 mL. OECN has a larvicidal action and antifungal activity at the tested concentrations.

Kajian Minyak Serai Wangi (Cymbopogon nardus L.) terhadap Aedes aegypti

<p>The <em>Aedes aegypti</em> mosquito can develop an immune system against the insecticide used. Control efforts with the use of insecticides are no longer effective if the target insects have become resistant. So there needs to be an effort to overcome the insect resistance that is targeted. Citronella grass is an herbal plant that can be used as an ingredient in vegetable pesticides. The leaves and stems are distilled to produce essential oil known as citronella oil. This research is about studying the effects of citronella oil against the resistance of the vector carrier for dengue fever, namely <em>Aedes aegypti. </em>The detection of citronella essential oil (<em>Cymbopogon nardus</em> L.) by Thin Layer Chromatography showed a reduction in UV₂₅₄ visualization (Rf 0,68). The resistance test showed that the mortality percentage of adult mosquitoes <em>Aedes aegypti</em> exposed to 0.03% alphacymethrin was 73,7% resistant criteria (&lt;90%). The mortality percentage of adult mosquitoes <em>Aedes aegypti</em> exposed to Alphacypermethrin (0,03%) and citronella oil was 2,5% each; 5%; 7,5% and 10% with treatment time 15 respectively, namely 18,7%, 50,7%, 70,7% and 82,7%; the treatment time of 30 minutes consecutively is 62,7%, 89,3%, 96% and 97,3% and the treatment time is 60 minutes respectively, namely 93,3%, 98,7%, 98,7% and 100%. There was an increase in the percentage of mosquito deaths exposed to the combination of alphacypermethrin and citronella oil, compared to those without citronella oil, this indicates that mosquitoes are more susceptible and resistance has decreased due to exposure to citronella oil. <strong></strong></p>

Antiseptic gel formulated from ethanol extract of Citronella grass (Cymbopogon nardus) using CMC-Na, arabic gums, and gelatin as gelling agents

Introduction: The ethanol extract from citronella grass (Cymbopogon nardus) has been found to contain saponins, phenolics (flavonoids), and polyphenols which have antibacterial activity. As a result, researchers developed an antiseptic gel that contains this ethanol extract. Aims: This study aims to find the optimum concentration of gelling agent required to make the gel and to use physical evaluations in order to understand whether combining gelling agents may increase the quality of the gel. Methods: The gel was made using a melting method, which mixed the base of gel with citronella ethanol extract at a temperature of 40oC to form a homogeneous phase. The gel evaluation was conducted using an organoleptic test, homogeneity test, dispersion test, pH test, and adhesion test. Results: The tests were carried out on 15 formulations respectively (R1, R2, and R3), and resulted in the production of clear, translucent yellow gel with a distinctive citronella grass odor. The homogeneity test showed that all formulations were homogeneous and contained no agglomerated particles. The gel dosage forms made with CMC-Na, gum arabic, and gelatin as gelling agents resulted in having a pH of 7 whilst gels made with a combination of gelling agents resulted in having a pH of 8. The combination of gum arabic and CMC-Na gelling agents showed an increase in spreadability of gel formulas at the same concentration of composition, of which the combinations were 1.25% gum arabic and 1.25% CMC-Na. The gel adhesion time was 0.2-2 minutes for all formula. Conclusion: Based on this test data, it can be concluded that the 15 gel formulations that resulted from this research are good and further testing can be performed to determine the most optimum and stable formula.

Antioxidant and Antimicrobial Properties of Ocimum sanctum and Cymbopogon nardus

Plant extracts have gained popularity recently, for their importance as potential antioxidative and antimicrobial agents. These properties have been attributed to their phytochemical content. The extraction solvent and the plant part are among the factors that influence the yield of these phytochemicals This study was therefore undertaken to investigate the antimicrobial and antioxidant activities of extracts of the leaf and stem of Ocimum sanctum (holy basil) and Cymbopogon nardus (citronella grass); two commonly occurring plants in South East Asia. The extracts were obtained by solvent extraction using water, methanol and ethanol. The percentage yield, antimicrobial activity, antioxidant activity and high-performance liquid chromatography (HPLC) analysis was investigated. The organic extracts yielded a higher percentage recovery of phytochemicals compared to the water extracts. HPLC analysis revealed the presence of chlorogenic acid in all extracts; rutin only in the leaf extracts and the citronella grass leaf extract contained both rutin and gallic acid. Antimicrobial assays were performed using the agar well diffusion method with tetracycline as positive control. Basil extracts exerted a greater inhibitory growth on both S. aureus and E. coli. S. aureus was found to be more susceptible to the presence of plant extracts. Water extracts did not display any zones of inhibition. The DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was used to study the antioxidant effect with Vitamin C (0.1mg/ml) as positive control. The results indicated that the Basil leaf extracts possessed greater antioxidant potential compared to the stem. The study concludes that organic extracts of O. sanctum and C. nardus possess pharmaceutical properties.

Fumigant and feeding deterrent activity of essential oils against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae)

Ikawati S, Himawan T, Abadi AL, Tarno H. 2020. Fumigant and feeding deterrent activity of essential oils against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae). Biodiversitas 21: 4301-4308. This study aimed to determine the fumigant and antifeedant activity of some plant essential oils against Cryptolestes ferrugineus. Essential oils were extracted from cinnamon (Cinnamomum verum), kaffir lime (Citrus hystrix), citronella grass (Cymbopogon nardus), zodea (Euodia suaveolens), and clove (Syzygium aromaticum). The extraction used the maceration method with n-Hexane as a solvent. The results of the experiments showed that all essential oils have fumigant toxicity. Fumigant toxicity of kaffir lime, citronella grass, zodea, and clove oil; eggs and pupae were more susceptible than adults and larvae, while for cinnamon, pupae and adults were more susceptible than eggs and larvae. Median Lethal Concentration (LC50) of all essential oils for eggs larvae, pupae, and adults respectively, for cinnamon were 17, 24, 9, and 12 ppm, for kaffir lime were 12, 17, 8 and 15 ppm, however, on citronella grass were 11, 22, 8, and 14 ppm, for zodea were 16, 22, 10 and 20 ppm, for clove were 11, 24, 7 and 14 ppm. The fifth essential oils showed antifeedant activity in a no-choice test against C. ferrugineus adults. Also, the fifth essential oils significantly reduced the relative growth rate (RGR), relative consumption rate (RCR), and food utilization (ECI) of C. ferrugineus adults. It shows that the essential oils have great potential as one of the storage pest control components.

EFEK KALIUM TERHADAP PERTUMBUHAN, PRODUKSI DAN MUTU SERAIWANGI / Effect of Potassium on Growth, Yield and Quality of Citronella Grass

<p>Citronella grass nowadays are developed in the highland that dominated by Andosol soil type. Thus, it is important to find out the proper fertilizer dosage, especially potassium, for citronella grass cultivation in Andosol soil. This study aimed to obtain the optimal of potassium dosage for growth, production and quality of Citronella grass oil. It was conducted at Manoko research station in Lembang, Bandung West Java from July 2014 to August 2015. This research was arranged in randomized block design, with 5 treatments and 5 replication. The treatments consisted of 5 potassium dosages: 1) without potassium, 2) 30 kg/ha K₂O, 2) 60 kg/ha K₂O, 3) 90 kg/ha K₂O, and 5) 120 kg/ha K₂O. The application of potassium on Andosol enhanced plant growth, production and oil yield significantly. However, it did not affect the quality of citronella grass oil (citronella and oil content) significantly. The application of 30 kg/ha K₂O in citronella grass cultivation on Andosol has the best result. The oil yield was 1.20% (the 1^st harvest), 1.28% (The 2^nd harvest) and 1.23% (the 3^rd harvest), with yield increment 6.9-7.5%. Oil content was 1,40-1,70%. The K nutrient uptake also improved around 11.76%. Citronella content from all treatments ranged from 36.97-37.68%. This result suggested that the low dosage of potassium in Andosol was sufficient to enhance primary metabolites process (indicated by growth and yield). However, it had no direct effect on the secondary metabolite process (represented by essential oil and citronella content). Based on the soil analysis after trial, the K residues in the soil was still high. However, its availability for the plants had to be tested further.</p><p><strong>Keywords: </strong><em>Cymbopogon nardus</em>, citronella content, oil content, oil yield, potassium.</p><p> </p><p><strong>Abstract</strong></p><p class="BodyText22">Pengembangan tanaman seraiwangi saat ini cenderung ke daerah-daerah dataran tinggi yang umumnya didominasi oleh tanah Andosol, oleh sebab itu perlu diketahui kebutuhan pupuk terutama kalium untuk tanaman seraiwangi di daerah tersebut. Penelitian ini bertujuan untuk memperoleh dosis K yang optimal untuk pertumbuhan, produksi dan mutu minyak sitronela yang dihasilkan dari tanaman seraiwangi. Penelitian ini telah dilakukan di Kebun Percobaan Manoko Balittro Lembang, Jawa Barat, dimulai dari Juli 2014-Agustus 2015. Penelitian ini menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan. Perlakuan terdiri dari 5 dosis K yaitu: 1) tanpa kalium (K1), 2) dosis 30 kg/ha K₂O, 3) dosis 60 kg/ha K₂O, 4) dosis 90 kg/ha K₂O, dan 5) dosis 120 kg/ha K₂O. Hasil penelitian menunjukkan bahwa pemberian kalium pada tanah Andosol berpengaruh terhadap peningkatan pertumbuhan tanaman, produksi dan rendemen, namun tidak mempengaruhi mutu minyak seraiwangi yang dihasilkan (kadar sitronela dan kadar minyak). Pemberian K sebanyak 30 kg/ha merupakan dosis terbaik dalam budidaya seraiwangi pada tanah Andosol. Rendemen yang diperoleh 1,20% (panen ke-1), 1,28% (panen ke-2) dan 1,23% (panen ke-3) dengan peningkatan sekitar 6,9-7,5%. Kadar minyak yang dihasilkan 1,40-1,70%. Peningkatan serapan hara K yang diperoleh sebesar 11,76%, dengan kadar sitronela 37,68%. Dengan demikian pemberian K pada tanah Andosol sangat diperlukan dalam proses metabolit primer (pertumbuhan dan produksi) dalam jumlah yang tidak terlalu tinggi, namun tidak berpengaruh langsung terhadap proses pembentukan metabolit sekunder (kadar minyak dan kadar sitronela). Selain itu kadar K yang tersisa di dalam tanah masih cukup tinggi, namun perlu dilakukan pengujian lebih lanjut mengenai ketersediaannya bagi tanaman.</p><p><strong>Kata kunci</strong>: <em>Cymbopogon nardus</em>, kalium, kadar minyak, rendemen minyak, sitronela</p><p class="BodyText22"><span lang="EN-US"><br /></span></p>