scholarly journals The Mosquito Larvicidal Activity of Essential Oils from Cymbopogon and Eucalyptus Species in Vietnam

Insects ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 128 ◽  
Author(s):  
Ho Dung Manh ◽  
Do Thi Hue ◽  
Nguyen Thi Thanh Hieu ◽  
Doan Thi Thanh Tuyen ◽  
Ong Thi Tuyet
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Eucalyptus Camaldulensis ◽  
Probit Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Eucalyptus Species ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Cymbopogon Winterianus

The larvicidal activity of essential oils (EOs) extracted from Cymbopogon citratus, Cymbopogon winterianus, Eucalyptus citriodora, and Eucalyptus camaldulensis aromatic plants grown in Vietnam was evaluated on Aedes aegypti larvae. The EOs were hydro-distilled in a Clevenger-type apparatus. The EOs were analyzed by gas chromatography–mass spectrometry (GC–MS). The mortality rates obtained from the bioassays were used to calculate the lethal concentrations (LC50) of the EOs by the probit analysis method. These essential oils exhibited toxicity to the larvae of Aedes aegypti. Results were obtained for Cymbopogon citratus (LC50 = 120.6 ppm), Cymbopogon winterianus (LC50 = 38.8 ppm), Eucalyptus citriodora (LC50 = 104.4 ppm), and Eucalyptus camaldulensis (LC50 = 33.7 ppm). The essential oils of Eucalyptus camaldulensis and Cymbopogon winterianus were found to be the most efficient, and their respective values of LC50 were 33.7 ppm, 38.8 ppm. In conclusion, this research adds to the growing body of literature on natural larvicides from essential oils against Aedes aegypti mosquitoes.

scholarly journals Evaluation of In Vitro Activity of Essential Oils againstTrypanosoma brucei bruceiandTrypanosoma evansi

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Nathan Habila ◽  
Abel S. Agbaji ◽  
Zakari Ladan ◽  
Isaac A. Bello ◽  
Emmanuel Haruna ◽  
...  
Keyword(s):  
Essential Oils ◽  
Eucalyptus Camaldulensis ◽  
Trypanosoma Evansi ◽  
In Vitro Activity ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Trypanosoma Brucei Brucei ◽  
Short Period ◽  
Dose Dependent

Essential oils (EOs) fromCymbopogon citratus(CC),Eucalyptus citriodora(EC),Eucalyptus camaldulensis(ED), andCitrus sinensis(CS) were obtained by hydrodistillation process. The EOs were evaluated in vitro for activity againstTrypanosoma brucei brucei(Tbb) andTrypanosoma evansi(T. evansi). The EOs were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. The drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/mL, and 0.1 g/mL for all the EOs. The concentration of 0.4 g/mL CC was more potent at 3 minutes and 2 minutes for Tbb andT. evansi, respectively. The GC-MS analysis of the EOs revealed presence of Cyclobutane (96.09%) in CS, 6-octenal (77.11%) in EC, Eucalyptol (75%) in ED, and Citral (38.32%) in CC among several other organic compounds. The results are discussed in relation to trypanosome chemotherapy.

scholarly journals EVALUATION OF THE INSECTICIDAL ACTIVITIES OF THREE EUCALYPTUS SPECIES CULTIVATED IN IRAN, AGAINST HYPHANTRIA CUNEA DRURY (LEPIDOPTERA: ARCTIIDAE)

2013 ◽  
Vol 53 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Asgar Ebadollahi ◽  
Mehdi Rahimi-Nasrabadi ◽  
Hossein Batooli ◽  
Jafar Geranmayeh
Keyword(s):  
Essential Oils ◽  
Larvicidal Activity ◽  
Exposure Time ◽  
Insecticidal Activity ◽  
Leaf Disc ◽  
Probit Analysis ◽  
Eucalyptus Species ◽  
Hyphantria Cunea ◽  
Insecticidal Activities

Abstract In the current study, the larvicidal activity of leaf essential oils from three eucalyptus species (Eucalyptus largiflorens Meull, Eucalyptus oleosa Meull, and Eucalyptus spathulata Hook) against American white moth, Hyphantria cunea Drury 1773 (Lepidoptera: Arctiidae), was investigated. Mortality was recorded daily for three days after treatment. Leaf disc bioassays revealed that all three oils had strong insecticidal activity on the experimental insects insofar as 50% lethal concentrations (LC50) for E. oleosa, E. spathulata, and E. largiflorens at 24 h exposure time were 0.36, 0.61, and 1.24%, respectively. The time needed to kill 50% (LT50) values were calculated as 9.09 h with E. largiflorens, 11.03 h with E. oleosa, and 13.03 h with E. spathulata at the highest concentrations (2.5% for E. largiflorens, 2% for E. oleosa, and 2.5% for E. spathulata). Based on probit analysis, an increase in the susceptibility of the insect was associated with an increase in the different concentrations of all oils and the increase in the time of exposure. The results of this study show that leaf essential oils of E. largiflorens, E. oleosa, and E. spathulata might be considered as a potent source for the production of fine natural larvicides.

Mosquito larvicidal activity of essential oils of Cymbopogon citratus and Thymus vulgaris grown in Cameroon

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
EG Nkouaya Mbanjo ◽  
F Tchoumbougnang ◽  
PM Jazet Dongmo ◽  
ML Sameza ◽  
PH Amvam Zollo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Larvicidal Activity ◽  
Thymus Vulgaris ◽  
Cymbopogon Citratus ◽  
Mosquito Larvicidal Activity

scholarly journals Larvicidal Activity of essential oils from Brazilian plants against Aedes aegypti L.

2004 ◽  
Vol 99 (5) ◽  
pp. 541-544 ◽  
Author(s):  
Eveline Solon Barreira Cavalcanti ◽  
Selene Maia de Morais ◽  
Michele Ashley A Lima ◽  
Eddie William Pinho Santana
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity

scholarly journals Antimicrobial Activity of Several Cineole-Rich Western Australian Eucalyptus Essential Oils

2018 ◽  
Vol 6 (4) ◽  
pp. 122 ◽  
Author(s):  
Fahad Aldoghaim ◽  
Gavin Flematti ◽  
Katherine Hammer
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Antimicrobial Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Eucalyptus Species ◽  
Broth Microdilution ◽  
Main Component ◽  
Western Australian

Essential oils from the Western Australian (WA) Eucalyptus mallee species Eucalyptus loxophleba, Eucalyptus polybractea, and Eucalyptus kochii subsp. plenissima and subsp. borealis were hydrodistilled from the leaves and then analysed by gas chromatography–mass spectrometry in addition to a commercial Eucalyptus globulus oil and 1,8-cineole. The main component of all oils was 1,8-cineole at 97.32% for E. kochii subsp. borealis, 96.55% for E. kochii subsp. plenissima, 82.95% for E. polybractea, 78.78% for E. loxophleba 2, 77.02% for E. globulus, and 66.93% for E. loxophleba 1. The Eucalyptus oils exhibited variable antimicrobial activity determined by broth microdilution, with E. globulus and E. polybractea oils showing the highest activities. The majority of microorganisms were inhibited or killed at concentrations ranging from 0.25% to 8.0% (v/v). Enterococcus faecalis and Candida albicans were the least susceptible organisms, whilst Acinetobacter baumannii was the most sensitive. In conclusion, all oils from WA Eucalyptus species showed microorganism inhibitory activity, although this varied according to both the Eucalyptus species and the microorganism tested. These data demonstrate that WA Eucalyptus oils show activity against a range of medically important pathogens and therefore have potential as antimicrobial agents.

scholarly journals AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
I Nyoman Pugeg Aryantha ◽  
Wahyu Setyaji Dwiantara
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Beauveria Bassiana ◽  
Larvicidal Activity ◽  
Control Agent ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Potato Dextrose Broth ◽  
Synthetic Pesticides ◽  
Biological Insect Control

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.   

scholarly journals Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

2014 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Rahmawati Ekaputri ◽  
Sudarsono Sudarsono ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Fruit Extract ◽  
Vinca Rosea ◽  
Layer Chromatography

<p>Background: <em>Vinca rosea</em> is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of <em>Vinca rosea</em> was selected to investigation its larvicidal activity against the 3<sup>rd</sup> instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) <em>Aedes aegypti.</em>  Five concentrations of Vinca fruit extract were tested against the 3<sup>rd</sup> instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC<sub>50</sub> anf LC<sub>90</sub>) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC<sub>50</sub> and LC<sub>90</sub> values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.</p>

Comparative Larvicidal Activity of Essential Oils from Three Medicinal Plants against Aedes aegypti L.

2010 ◽  
Vol 7 (11) ◽  
pp. 2801-2807 ◽  
Author(s):  
Flávia P. Freitas ◽  
Silvério P. Freitas ◽  
Gloria C. S. Lemos ◽  
Ivo J. C. Vieira ◽  
Geraldo A. Gravina ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity

scholarly journals Chemical Composition and Larvicidal Activity against Aedes Aegypti of Essential Oils from Croton Zehntneri

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Hélcio S. Santos ◽  
Gilvandete M. P. Santiago ◽  
João P. P. de Oliveira ◽  
Angela M. C. Arriaga ◽  
Délcio D. Marques ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Plant Parts ◽  
Main Component

The chemical composition of the essential oils from leaves, stalks and inflorescences of Croton zehntneri obtained by hydrodistillation were analyzed by GC-MS and CG-FID. E-Anethole was the main component of the essential oils of all plant parts. Essential oils of leaves, stalks, inflorescences and E-anethole were tested at different concentrations against instar III larvae of Aedes aegypti and showed LC50 values of 56.2 ± 0.3, 51.3 ± 0.3, 57.5 ± 0.1 and 69.2 ± 0.5 μg/mL, respectively.

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