Toxicity Assessment of Different Solvent Extracts of the Medicinal Plant, Phragmanthera capitata (Sprengel) Balle on Brine Shrimp (Artemia salina)

2016 ◽  
Vol 12 (7) ◽  
pp. 701-710 ◽  
Author(s):  
Franklin Uangbaoje Ohikhena ◽  
Olubunmi Abosede Wintola ◽  
Anthony Jide Afolayan
Keyword(s):  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Toxicity Assessment

scholarly journals Skrining Fitokimia dan Uji Aktivitas Sitotoksik dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Modustriati P. Mardany ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Active Compound ◽  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Test Method ◽  
High Cytotoxic Activity

Sarang semut (Myrmecodia beccarii Hook.f.) is a famous medicinal plant of Papua, which has traditionally potential in treating a wide variety of mild and severe illnesses, such as cancers and tumors, gout, coronary heart disease, hemorrhoids, tuberculosis, migraines, rheumatism, and leukemia. The purposes of this study were to determine the content of the active compound group and the cytotoxic activity of sarang semut plants from Merauke Region. The extraction was done by maceration using 96 % ethanol. The concentration of ethanolic extract were 0, 25, 50, 75, 100 ppm. The results of the study showed that the cytotoxic activity determine using BSLT (Brine Shrimp Lethality Test) method have a high cytotoxic activity with LC50 values of 22.86 ppm and containing the active compound namely flavonoids, tannins and saponins separately. The ethanolic extract of tuber of sarang semut (M. beccarii) has a high cytotoxic activity against larvae of Artemia salina Leach according BSLT method. Key words: Active compound, M. beccarii, cytotoxic activity, Merauke.

scholarly journals Uji Toksisitas Ekstrak Batang Pinang Yaki (Areca vestiaria) pada Artemia salina Leach.

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Windy Astuti Tampungan
Keyword(s):  
Medicinal Plant ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Toxicity Level ◽  
Supernatant Solution

AbstrakEfek toksisitas pinang yaki (Areca vestaria) sebagai tumbuhan obat potensial perlu diuji untuk mengetahui ambang batas penggunaannya. Penelitian ini bertujuan untuk menguji tingkat toksisitas ekstrak batang pinang yaki terhadap larva Artemia salina Leach. dengan metode Brine Shrimp Lethality Test. Ekstrak diencerkan dengan konsentrasi 200, 400, 600 dan 800 ppm masing-masing untuk supernatan rendaman pertama (larutan A),supernatan rendaman ke dua (larutan B) dan supernatan rendaman ke tiga (larutan C) . Hasil penelitian menunjukkan bahwa ekstrak batang pinang yaki mempunyai efek toksisitas dengan nilai LC50 sebesar 398,28 ppm untuk larutan A, 390,84 ppm untuk larutan B dan 438,53 ppm untuk larutan C.Kata kunci: batang Areca vestaria,LC50, uji toksisitasAbstractToxicity effect of pinang yaki (Areca vestiaria) as potential medicinal plant should be evaluated to know its application threshold. This research aimed to evaluate the toxicity level of pinang yaki trunk extract on larve of Artemia salina Leach. using the method of Brine Shrimp Lethality Test. Extract was diluted to concentration of 200, 400, 600 and 800 ppm each for solution A (first soaking supernatant), solution B (second soaking supernatant)and solution C (third soaking supernatant). The result of this research showed that extract of pinang yaki trunk had a toxic characteristic, with LC50 value was 398,28, 390,84, and 438,53 ppm respectively for solution A, B and C.Keywords: LC50 , toxicity test, trunk of Areca vestaria

scholarly journals TOXICITY TEST (LD-50) of HERBAL PREPARATIONS Nepenthes ampularia AS The STANDARD of SECURITY of JAMU HYPERTENSION

2021 ◽  
Vol 748 (1) ◽  
pp. 012029
Author(s):  
Mardhiana ◽  
Abdul Rand ◽  
K Harlinda
Keyword(s):  
Medicinal Plant ◽  
Empirical Evidence ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Death Rate ◽  
Artemia Salina ◽  
Toxic Effects ◽  
Water Safety ◽  
Brine Shrimp Lethality ◽  
Herbal Preparations

Abstract The excavation of the potential utilization of herbs as a plant biopharma or as a material to manufacture. There is empirical evidence that Nepenthes has been used as a lowering of hypertension by taking a decoction of its pitchers. For the use and utilization as a medicinal plant need to be done research that refers to the scientifically certified Jamu. The research aims to detect acute toxic effects so that can be obtained the water safety overview Nepenthes pitchers. The toxicity test was carried out by the Brine Shrimp Lethality Test (BSLT), Artemia salina larva with four extract concentrations (0, 10, 500, 1000 ppm). Based on the results of toxic tests showed that the death rate of A. salina at all levels of concentration is 0 deaths. It is evidence that Nepenthes ampularia is not toxic to A. salina..

scholarly journals UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2020 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Arinta Mayang ◽  
Bilal S A Santoso
Keyword(s):  
Acute Toxicity ◽  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Chemical Contents

ABSTRACTAnnona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsakleaves contain alkaloids, tannins, and several other chemical contents including acetogenin.The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves onthe Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimentalstudy used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemiasalina larvae used for each concentration was 10 larvae. The number of dead larvae wascounted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leafinfusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves istoxic.Keywords: Sirsak leaves, toxicity, BSLTABSTRAKSirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanamanobat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasukacetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infusdaun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larvaArtemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva matidihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsakadalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.Kata kunci: daun sirsak, toksisitas, BSLT

Toxicity assessment of Lavandula officinalis extracts in Brine Shrimp (Artemia salina)

2019 ◽  
Vol 29 (6) ◽  
pp. 411-420
Author(s):  
Welligton Luciano Braguini ◽  
Bruno Bianchin Alves ◽  
Natália Valendolf Pires
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Toxicity Assessment ◽  
Lavandula Officinalis

scholarly journals Antioxidant Properties and Toxicity Assessment of the Crescentia cujete Extracts in Brine Shrimp (Artemia salina)

2019 ◽  
Vol 48 (4) ◽  
pp. 831-840
Author(s):  
Mozhiarhasi Sandra Sagrin ◽  
Nur Fatimah Lasano ◽  
Radhiah Shukri ◽  
Nurul Shazini Ramli
Keyword(s):  
Brine Shrimp ◽  
Antioxidant Properties ◽  
Artemia Salina ◽  
Toxicity Assessment ◽  
Crescentia Cujete

scholarly journals Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant Palicourea rigida Kunth (Rubiaceae): Detailed Biological Activities

2021 ◽  
Vol 7 (9) ◽  
pp. 689
Author(s):  
Igor Romeiro dos Santos ◽  
Ahmed M. Abdel-Azeem ◽  
Marwa T. Mohesien ◽  
Magdalena Piekutowska ◽  
Donia H. Sheir ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Artemia Salina ◽  
Toxicity Assessment ◽  
Industrial Applications ◽  
Phytopathogenic Fungi ◽  
Brazilian Savanna ◽  
Palicourea Rigida ◽  
Agricultural Applications

A multitude of plants from the Brazilian savanna are known for their medicinal properties. Many plants contain endophytic fungi, which lead to the production of bioactive compounds by both the fungi and their hosts. This study investigated the bioprospecting of endophytic fungi recovered from the leaves of Palicourea rigida, a native medicinal plant of the Brazilian savanna. Four fungal taxa (Colletotrichum sp. SXS649, Pestalotiopsis sp. SXS650, the order Botryosphaeriales SXS651, and Diaporthe sp. SXS652) were recovered. The phenolic, flavonoid, extracellular degrading enzymes (amylase, cellulase, protease, and tannase) and antioxidant activity of these taxa were determined. Evaluation of the antimicrobial activity showed that the Botryosphaeriales SXS651 extract displays a minimum inhibitory concentration (MIC) of 23.20 mg mL−1 against Staphylococcus epidermidis and Pseudomonas aeruginosa, and the Diaporthe sp. SXS652 extract exhibited an MIC of 27.00 mg mL−1 against Escherichia coli. The Colletotrichum sp. SXS649 isolate inhibited tumors in potato discs by 69% at a concentration of 9.70 mg mL−1. All isolates had potential bioremediation criteria against soil contaminated with soybean oil, as proved by a high percentage of germination of Lactuca sativa and a reduction in phytotoxicity. Furthermore, the taxa under investigation demonstrated antagonistic action to phytopathogenic fungi, namely, Aspergillus niger, Inonotus rickii, Pestalotiopsis mangiferae, and Coniophora puteana, with an inhibition range between 34.2% and 76.9%. The preliminary toxicity assessment showed that all isolates possessed an LC50 of less than 100 mg mL−1 to the microcrustacean Artemia salina. These results indicate that the endophytic fungi of the Brazilian savanna are promising candidates for biotechnological and industrial applications and, in agricultural applications, for the biological control of phytopathogenic fungi.

Biosynthesis of the Diguanosine Nucleotides. I. Purification and Properties of an Enzyme from Yolk Platelets of Brine Shrimp Embryos

1974 ◽  
Vol 52 (3) ◽  
pp. 231-240 ◽  
Author(s):  
A. H. Warner ◽  
P. C. Beers ◽  
F. L. Huang
Keyword(s):  
Molecular Weight ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Temperature Optimum ◽  
Small Molecular Weight ◽  
Ph Optimum ◽  
Optimal Activity ◽  
Purification And Properties

An enzyme that catalyzes the synthesis of P1P4-diguanosine 5′-tetraphosphate (Gp4G) has been isolated and purified from yolk platelets of encysted embryos of the brine shrimp, Artemia salina. The enzyme GTP:GTP guanylyltransferase (Gp4G synthetase) utilizes GTP as substrate, has a pH optimum of 5.9–6.0, a temperature optimum of 40–42 °C, and requires Mg2+ and dithiothreitol for optimal activity. The synthesis of Gp4G is inhibited markedly by pyrophosphate, whereas orthophosphate has no effect on the reaction. In the presence of GDP the enzyme also catalyzes the synthesis of P1,P3-diguanosine 5′-triphosphate (Gp3G), but the rate of synthesis is low compared with Gp4G synthesis and dependent upon other small molecular weight components of yolk platelets.

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