Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples

2011 ◽  
Vol 6 (11) ◽  
pp. 951-962 ◽  
Author(s):  
Giorgia Bottini ◽  
Francesca Losito ◽  
Alessio De Ascenti ◽  
Francesca Romana Priolisi ◽  
Alberto Mari ◽  
...  
Keyword(s):  
Food Samples ◽  
Viable Count ◽  
Survey Method ◽  
Total Viable Count ◽  
E Coli ◽  
Biological Survey

scholarly journals Characterization and Antimicrobial Resistance of Escherichia coli and Its Serotypes Isolated From Poultry Feed in Relation to Seasons in Karachi, Pakistan

2020 ◽  
Vol 12 (4) ◽  
Author(s):  
Shajeela Iram ◽  
Mehmood ul Hassan ◽  
Tabinda Khawaja
Keyword(s):  
Escherichia Coli ◽  
Biochemical Characterization ◽  
Bacterial Load ◽  
Viable Count ◽  
Poultry Feed ◽  
Diffusion Method ◽  
Total Viable Count ◽  
E Coli ◽  
Resistant Strains ◽  
Feed Samples

Background: The increasing prevalence of Escherichia coli as a foodborne pathogen in poultry poses a high risk to food safety. The resistant strains of E. coli may contribute resistant genes to human endogenous flora, causing fatal diseases. Moreover, these pathogens are serious threats to poultry farming. Objectives: The current study aimed to investigate the effects of seasonal variations on the bacterial load of E. coli and to evaluate its antibiogram profile. Methods: All feed samples were evaluated for the identification of E. coli and its serotypes, using conventional culture methods and biochemical characterization. Positive samples were confirmed by polymerase chain reaction (PCR) assay. The bacterial load of E. coli was estimated by measuring the total viable count, and the antibiogram data were calculated using two methods, that is, disc diffusion method and minimum inhibitory concentration (MIC) measurement. Results: Of 204 feed samples investigated, 38 isolates were positive for E. coli. All positive samples were also confirmed via universal and species-specific PCR assays, and 8/38 were documented as E. coli 0157:H7 strains. The bacterial load of E. coli was also determined by measuring the total viable count, and the results revealed the highest ratio (6.44×108 CFU/g) from June to August and the lowest ratio (2.06×108 CFU/g) from December to February. The multidrug resistance of E. coli O157:H7 was validated by antimicrobial susceptibility tests since all isolates showed high resistance to chloramphenicol, penicillin derivatives, fluoroquinolones, and oxytetracycline, respectively, and were only susceptible to aminoglycosides. Conclusions: Considering the high bacterial load of E. coli from June to August, the poultry industry needs to establish appropriate and effective hygienic and storage policies, especially during these alarming months. Moreover, surfacing and propagation of resistant strains of these pathogens may obscure future assessments for treatment purposes.

scholarly journals Study on Isolation and Identification of Salmonella and Escherichia coli from Different Poultry Feeds of Savar Region of Dhaka, Bangladesh

2011 ◽  
Vol 3 (2) ◽  
pp. 403-411 ◽  
Author(s):  
A. Chowdhury ◽  
A. Iqbal ◽  
M. G. Uddin ◽  
M. Uddin
Keyword(s):  
Escherichia Coli ◽  
Viable Count ◽  
Nutrient Agar ◽  
Effective Control ◽  
Microbiological Analysis ◽  
Total Viable Count ◽  
Isolation And Identification ◽  
Poultry Feeds ◽  
E Coli ◽  
Feed Samples

The study was conducted aiming at the isolation and identification of Salmonella and Escherichia coli (E. coli) from different brands of poultry feeds sold in Savar, Dhaka, Bangladesh. Seven different poultry feeds were subjected to microbiological analysis. All these samples were analyzed by culturing in different media such as nutrient broth (NB), nutrient Agar (NA), SS Agar (Salmonella-Shigella Agar), BGA (brilliant-green Agar), Mac Conkey, DHL and EMB (eosin methylene blue) media. Total bacterial colonies of all the samples were counted separately on the nutrient Agar media. Hence, bacteria were counted as 9.5×105 in the feed sample C (Layer) which was found to be the highest in number among the poultry feeds. Total viable count (TVC) of Salmonella and E. coli in the feed samples were as 0 to 6.75×104 and 0 to 3.05×104 respectively.  Both organisms were found in 71.43% and 57.14% of the analyzed feed samples, respectively. The highest number of Salmonella was found in sample C (Layer) feeds and that of E. coli was found in sample B (Grower) feeds. The widespread occurrence of Salmonella and E. coli in poultry feeds reinforces the need for effective control measures, hygiene in processing and handling of feeds. Keywords: Salmonella; Escherichia coli; Poultry feeds; Total viable count; Contamination; Hygiene. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi:10.3329/jsr.v3i2.7128                J. Sci. Res. 3 (2), 403-411 (2011)

An Australian National Survey of the Microbiological Quality of Frozen Boneless Beef and Beef Primal Cuts

2012 ◽  
Vol 75 (10) ◽  
pp. 1862-1866 ◽  
Author(s):  
DAVID PHILLIPS ◽  
KATHRYN BRIDGER ◽  
IAN JENSON ◽  
JOHN SUMNER
Keyword(s):  
Microbiological Quality ◽  
Biceps Femoris ◽  
Viable Count ◽  
Longissimus Dorsi ◽  
Total Viable Count ◽  
E Coli ◽  
The Mean ◽  
First Time ◽  
Coagulase Positive Staphylococci

The fourth national baseline microbiological survey of Australian beef was conducted in 2011, including frozen boneless beef and, for the first time, samples from selected beef primal cuts. Cartons of frozen boneless beef (n = 1,165) sampled at 29 boning (fabrication) plants were found to have a mean total viable count of 2.2 log CFU/g, and the mean count for the 2.1% of samples with detectable Escherichia coli was 1.3 log CFU/g. The mean total viable counts for striploins (longissimus dorsi, n = 572) and outsides (biceps femoris, n = 572) were 1.3 and 1.5 log CFU/cm2 respectively. E. coli isolates were obtained from 10.7 and 25.2% of striploins and outsides, respectively, with mean counts of −0.5 and −0.3 log CFU/cm2 on positive samples. E. coli O157:H7, Salmonella, and Campylobacter were not isolated from any primal cut samples, and Salmonella was not isolated from any of the boneless product (E. coli O157:H7 and Campylobacter were not tested for). Listeria spp. were not detected in any of the boneless product, and one Listeria isolate was obtained on 1 (0.2%) of 572 striploin samples. Coagulase-positive staphylococci were isolated from 3.4% of boneless beef samples, 7.7% of beef striploins, and 8.4% of beef outsides, with positive samples having mean log counts of 1.9 CFU/g, 0.2 CFU/cm2, and 0.2 CFU/cm2, respectively.

scholarly journals Microbial analysis of locally prepared vended dishes in owerri municipality of imo state, Nigeria

2020 ◽  
Vol 3 (10) ◽  
pp. 239-243
Author(s):  
Mercy Adaramaja. F
Keyword(s):  
Total Coliform ◽  
Food Samples ◽  
Viable Count ◽  
Gas Production ◽  
Total Viable Count ◽  
Mould Growth ◽  
White Rice ◽  
Imo State ◽  
Microbial Analysis ◽  
High Sense

A study was carried out to access the microbial analysis of locally prepared vended dishes in Owerri municipality of Imo state. Five zones namely: Prefab and Aladinma areas are represented as Zone A while Ikenegbu and Wethdral areas, Douglas area, School road and Tetlow areas and Worldbank area represented Zone B, C, D and E respectively. The food samples collected includes; rice, spagehetti, beans and white stew. The result obtained revealed that the total viable count in zone A contained 11.42 × 103, 9.5 × 103, 4.4 × 104 and 10.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew while those of zone B are 9.0× 103, 7.70 × 103, 2.5× 104 and 6.0 × 105 (Cfu/mL) for white rice, spaghetti, beans and stew respectively. Zone C stew (10.4 ×103 cfu/mL), spaghetti (2.0× 104 cfu/mL), beans (3.3× 104 cfu/mL) and stew (10.3× 104 cfu/mL). Zone D stew (8.0 × 103 cfu/mL), spaghetti (1.5× 104 cfu/mL), beans (1.4× 104 cfu/mL) and stew (4.0×105cfu/mL). Zone E stew (12.55 × 103 cfu/mL), spaghetti (6.3× 103 cfu/mL), beans (6.0× 104 cfu/mL) and stew (4.5×105cfu/mL). Total coliform count shows no gas production in Durbam tube in all the zones, no mould were also observed in all the samples. Generally, mould growth on food is evidence deterioration. It was concluded that the zones maintained high sense of hygiene.

scholarly journals Physicochemical and Microbiological Qualities’ Assessment of Popular Bangladeshi Mango Fruit Juice

2018 ◽  
Vol 12 (1) ◽  
pp. 135-147 ◽  
Author(s):  
Ruhul Amin ◽  
Shafkat S. Rahman ◽  
Mahboob Hossain ◽  
Naiyyum Choudhury
Keyword(s):  
Viable Count ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Total Viable Count ◽  
Culture Techniques ◽  
E Coli ◽  
Safe Level ◽  
Microbial Risks ◽  
Mango Juice ◽  
Mango Juices

Introduction:Mango juice has always been considered as a delicious, nutritious popular drink, but processed juice may not always be safe due to chemical and microbial risks. Determination of physicochemical and microbiological qualities of some packed mango juices of Bangladesh will help consumers to know the present scenario.Material and Methods:Six commercially available different juice samples were collected from the market. Carbohydrate profiles were determined using HPLC, crude protein content was calculated using the Kjeldahl method and other parameters were determined by standard AOAC methods. Standard culture techniques were followed to assess the total viable count (TVC),E. coliand other fecal coliforms.Results:The highest quantity of monosaccharide (58.88%) was recorded in the AC1ME5 brand, while the lowest in Homemade (5.648%) and MN1GL2 (9.867%). The maximum content of acidity recorded was 0.24% and minimum 0.21%. The TSS content of all samples varied from 19% to 12%. The highest quantity 6.87% and the lowest 3.62% of reducing sugar were recorded. Most of the mango juices were low in protein and very low/negligible in fat content. Total viable count of different types of fruit juices varied from 1×103- 3×103CFU/ml. No significant amount ofE. coliand fecal coliform was detected.Conclusion:It can be concluded that the locally available mango juices contain a safe level of nutritional and microbial elements for human consumption, but not highly satisfactory.

scholarly journals Comparative microbiological analysis of raw fishes and sun-dried fishes collected from the Kawran bazaar in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (3) ◽  
pp. 846-851
Author(s):  
I.T. Nur ◽  
B. Ghosh ◽  
M. Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Quality Analysis ◽  
Microbiological Analysis ◽  
Total Viable Count ◽  
Salmonella Spp ◽  
E Coli ◽  
Coliform Count

Along with the raw fishes, dry fishes also have a huge contribution to meet up the demand of protein in our daily meal. The assay of microbiological quality is therefore needed to ensure the public health safety. The present study was emphasized on the existence of pathogenic bacteria in raw and dry fish. A total of 50 samples of raw fishes and sun-dried fishes was accumulated aseptically for microbiological quality analysis. Isolation of bacteria was done by spread plate method. All the samples including both (raw and dry) fishes harbored bacteria and fungi up to 106 CFU/g. E. coli was found in all samples as a specific pathogen. In case of raw fishes total viable count (TVC) and total coliform count (E. coli) were recorded up to 2.5x106 CFU/g and 5.2 x104 CFU/g respectively whereas a significant load of Salmonella spp. was observed in almost all samples. Staphylococcus spp. and Pseudomonas spp. were present up to 5 x102 CFU/g and 1.8 x 102 CFU/g respectively. Likewise, total viable count (TVC), total coliform count (E. coli) and fungal load were recorded in dry fish up 3.50 x 105 CFU/g, 1.2 x103 CFU/g respectively. Fungal growth was observed in all experimental raw and dried fishes. For most of the pathogenic isolates, higher rates of resistance were found against Ceftriaxone, Penicillin, Nalidixic acid, Neomycin. On the other hand, most of the isolates were found to retain higher sensitivity against Imipenem, Ciprofloxacin, Tetracyclin and Amoxicillin. This data suggested that the dry fish harbored fewer bacteria than raw fish and sun drying method is still a useful technique for the preservation of fish.

Investigation of microbial contamination of Powdered Infant Formula during different storage periods after opening

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Mohammed Khalid Al-atrash
Keyword(s):  
Infant Formula ◽  
Environmental Protection Agency ◽  
Microbial Contamination ◽  
Total Coliform ◽  
Viable Count ◽  
Risk Level ◽  
Powdered Infant Formula ◽  
Total Viable Count ◽  
Coliform Count ◽  
Significant Difference

The present study was carried out to knowing effect different storage periods of the microbial quality for the Powdered Infant Formula (PIF) after opening the tin, and ensuring from the safety note (after opening, use within 3 weeks). Thirty (30) samples of (PIF) from category (1 – 6 months) in five different types are collected from pharmacies and local markets in Baquba city / Iraq, which are used as substitutes for breast milk during the first day of opening the tin powders such as Total viable count, Total coliform count, Salmonella count and Yeast and Molds count. These experiments repeated at each week of same samples within (5) weeks. Results were obtained at opening the tin, Total viable count (less than 0.05 1.0 x 103 ±1.5x10 CFU/g) were significantly higher than Total Coliform count (less than 0.05 ± 0.3 x 10 CFU/g) and Total Salmonella count (less than 0.05 0 x 10 CFU/g) and Yeasts and Molds (less than 0.05 ± 0.3 x 10 CFU/g). while results obtained at fifth week were (less than 0.05 8.8 x 103 ±5.5x102 CFU/g), (less than 0.05 0.9 x 102 ± 0.4x101 CFU/g), (less than 0.05 0 x 10 CFU/g), (less than 0.05 9.5 x 10 ± 1.2x101 CFU /g) respectively. All samples of (PIF) having non-significant difference. These results compared to Iraqi Quality Standards (IQS), all the results from the opening samples to fifth week were within the range of IQS and USA Environmental Protection Agency (USEPA) and as indicates the hygienic condition of (PIF) without risk level for human health. also observed increase in microbial contamination in each week because increase the moisture content for powdered milk. Can be used more than 3 weeks after opening if stored in good conditions with good hygienic practices during milk preparation.

scholarly journals Comparison of Fecal Coliform Agar and Violet Red Bile Lactose Agar for Fecal Coliform Enumeration in Foods

2002 ◽  
Vol 68 (4) ◽  
pp. 1631-1638 ◽  
Author(s):  
A. Leclercq ◽  
C. Wanegue ◽  
P. Baylac
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Food Samples ◽  
Fecal Coliforms ◽  
Predictive Values ◽  
Direct Plating ◽  
Hafnia Alvei ◽  
E Coli ◽  
Plating Method ◽  
Mashed Potatoes

ABSTRACT A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 ± 1°C and transfer to 44 ± 1°C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.

Biodegradation Potential of Abattoir Wastewater Microbiota in Nigeria

2020 ◽  
Vol 15 (3) ◽  
pp. 70-77
Author(s):  
J.D Bala ◽  
F. A Kuta ◽  
N.U Adabara ◽  
O.P Abioye ◽  
H.S Auta ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Oxygen Demand ◽  
Total Suspended Solid ◽  
Sole Source ◽  
Suspended Solid ◽  
Viable Count ◽  
Total Viable Count ◽  
Slaughter House ◽  
Abattoir Wastewater ◽  
Total Dissolved Solid

Water used for washing carcasses of slaughtered animals and slaughter house is referred to as abattoir wastewater. This study was designed to investigate the microorganisms associated with abattoir wastewater and to establish the biodegradation potential of abattoir wastewater microbiota. Isolation of the microbes was carried out using pour plate technique. The total viable count for the microbes’ ranges from 2.5×104 - 4.6×105 cfu/mL. Results revealed that all the physicochemical parameters exceeded the permissible limits (total dissolved solid (TDS) 1748mg/L, total suspended solid (TSS) 176mg/L, biochemical oxygen demand (BOD5) 91 mg/L and chemical oxygen demand (COD) 227 mg/L). Microorganisms isolated include Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus anthracis, Aspergillus niger, A. flavus, Mucor sp, Trichophyton quickeanum and Penicillium sp. Some of the microbes were observed to have biodegradation potential by their ability to grow on mineral salt media (MSM) incorporated with starch, cellulose, crude oil, kerosene and diesel as the sole source of carbon and energy. This study suggests that abattoir wastewater harbors microorganisms that could be hazardous to public health when discharged into the environment untreated hence the need for strict monitoring. These microbes isolated could be employed as agent of bioremediation of wastewaters. Key words: Abattoir; Biodegredation; Isolation; Microbiota; Wastewater

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