Alternaria mycotoxins in grains from Albania: alternariol, alternariol monomethyl ether, tenuazonic acid and tentoxin

2019 ◽  
Vol 12 (1) ◽  
pp. 89-99 ◽  
Author(s):  
D. Topi ◽  
G. Tavčar-Kalcher ◽  
K. Pavšič-Vrtač ◽  
J. Babič ◽  
B. Jakovac-Strajn
Keyword(s):  
Adult Population ◽  
Daily Intake ◽  
Dietary Exposure ◽  
Monomethyl Ether ◽  
Occurrence Rate ◽  
Contamination Rate ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Daily Consumption ◽  
Alternaria Toxins

The presence of four Alternaria toxins in maize and wheat harvested in 2014 and 2015 in Albania was investigated. In total, 45 samples of maize and 71 samples of wheat were collected from the country’s main producing regions. The presence of alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA) and tentoxin (TTX) was studied by an LC-MS/MS method. The incidence of Alternaria toxins in maize was 45.2% in the year 2014 and 7.1% in 2015, and the contamination rate in wheat was 82.9% in 2014 and 86.1% in 2015. Considering maize and wheat samples together, 65.2 and 64.0% of samples were contaminated by Alternaria toxins in the harvesting years 2014 and 2015, respectively. The occurrence rate was much higher in wheat than in maize, but the concentrations were higher in maize. The highest concentration of total Alternaria toxins in maize was 1,283 μg/kg (mean 243.0 μg/kg, median 110.2 μg/kg), while the maximum concentration in wheat was 175.7 μg/kg (mean 29.9 μg/kg, median 16.5 μg/kg). TeA was the major Alternaria mycotoxin detected. It was found in 70 out of 116 samples (60.3%). Chronic exposure of the adult population in Albania to Alternaria toxins through cereal consumption was assessed by the estimated daily intake (EDI) taking into account daily consumption of wheat and maize of 380 and 4.9 g, respectively. The main contribution to chronic dietary exposure was by TeA originating from wheat, with EDIs of 88.6-94.1 ng/kg body weight (bw) per day in 2014 and 152.7-155.5 ng/kg bw per day in 2015. TTX EDIs were 7.8- 34.0 and 10.6-38.7 ng/kg bw per day in 2014 and 2015, respectively. The contribution of AOH and AME originating from wheat was 0-31.7 ng/kg bw per day. The contribution of Alternaria toxins through maize consumption was significantly lower.

scholarly journals Development and Application of a QuEChERS-Based Liquid Chromatography Tandem Mass Spectrometry Method to Quantitate Multi-Component Alternaria Toxins in Jujube

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 382 ◽  
Author(s):  
Cheng Wang ◽  
Yingying Fan ◽  
Weizhong He ◽  
Dongqiang Hu ◽  
Aibo Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Extraction Procedure ◽  
Monomethyl Ether ◽  
Tandem Mass ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Infection Level ◽  
Alternaria Toxins

A simple, rapid and efficient methodology was developed and validated for the analysis of four Alternaria toxins in jujube: Tenuazonic acid, alternariol, alternariol monomethyl ether, and tentoxin. Under the optimized extraction procedure, chromatographic conditions, and instrumental parameters, the four toxins were effectively extracted via a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method, and quantified by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Matrix-matched calibrations ranging from 0.01 to 0.5 μg mL−1 were conducted for the quantification due to the matrix effect. A blank jujube sample was spiked at 40, 80 and 160 μg kg−1, obtaining recoveries in the range of 83.5–109.6%. Limits of detection and limits of quantification were in the range of 0.14–0.26 and 0.47–0.87 μg kg−1, respectively. Finally, the developed method was applied for the quantification of the four toxins in 14 jujube samples, including black spot-infected and uninfected samples. Results showed that the predominant toxin detected in all the samples was tenuazonic acid, the content of which was associated with the infection level; alternariol, alternariol monomethyl ether, and tentoxin were detected in all the infected samples and some of the uninfected samples with rather low contents.

Alternaria Mycotoxins in Sunflower Seeds: Incidence and Distribution of the Toxins in Oil and Meal

1995 ◽  
Vol 58 (10) ◽  
pp. 1133-1134 ◽  
Author(s):  
SOFÍA N. CHULZE ◽  
ADRIANA M. TORRES ◽  
ANA M. DALCERO ◽  
MIRIAM G. ETCHEVERRY ◽  
MARÍA L. RAMÍREZ ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Sunflower Seed ◽  
Monomethyl Ether ◽  
Sunflower Seeds ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Laboratory Conditions ◽  
Alternaria Toxins ◽  
Alternaria Mycotoxins

A survey of 150 sunflower-seed samples was carried out to evaluate the contamination from infection with Alternaria alternata with alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA). A high percentage of the samples was contaminated with AOH (85%), AME, (47%), and TA (65%). The average levels detected were 187 μg/kg for AOH, 194 μg/kg for AME, and 6,692, μg/kg for TA. When sunflower seeds fermented by Alternaria alternata were processed under laboratory conditions to obtain the oil and meal, different distributions of Alternaria toxins between the oil and the meal were observed: whereas AOH, AME, and TA were detected in the meal, only AME and TA were detected in the oil, and the latter in a low percentage.

scholarly journals Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough

2020 ◽  
Vol 8 (2) ◽  
pp. 303
Author(s):  
Elizabet Janić Hajnal ◽  
Lato Pezo ◽  
Dejan Orčić ◽  
Ljubiša Šarić ◽  
Dragana Plavšić ◽  
...  
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Regression Function ◽  
Monomethyl Ether ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Whole Wheat Flour ◽  
Wheat Dough ◽  
Whole Wheat ◽  
Alternaria Toxins

The aim of this study was to investigate the fate of the most common Alternaria toxins found in wheat—tenuazonic acid (TeA), alternariol (AOH), and alternariol monomethyl ether (AME) during sourdough processing. For this purpose, spiked whole wheat flour, 3% sourdough starter, 0.5% of baker’s yeast, and 105% of water calculated on flour weight as a base were used as raw materials. Spiked whole wheat dough was fermented for 4 h, 8 h, 12 h, 24 h, and 48 h at 25 °C, and at each point the fermented dough samples were taken, frozen, lyophilized, grounded, and stored until further analysis. To study the effect of sourdough processing on TeA, AOH and AME content, the validated method of high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for these mycotoxins was used. Mathematical models of Alternaria toxins reduction were developed in the form of Four Parameter Logistic Regression function. The maximum reduction of TeA, AOH, and AME levels was archived at 48 h of dough fermentation at 25 °C compared with dough after kneading (0 h). Under these conditions, a reduction of the toxin levels of 60.3 %, 41.5%, and 24.1% was observed for TeA, AOH, and AME, respectively.

scholarly journals Mycotoxin Dietary Exposure Assessment through Fruit Juices Consumption in Children and Adult Population

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 684 ◽  
Author(s):  
Noelia Pallarés ◽  
Dionisia Carballo ◽  
Emilia Ferrer ◽  
Mónica Fernández-Franzón ◽  
Houda Berrada
Keyword(s):  
Fruit Juice ◽  
Adult Population ◽  
Caloric Intake ◽  
Fumonisin B1 ◽  
Dietary Exposure ◽  
Monomethyl Ether ◽  
Alternariol Monomethyl Ether ◽  
Fumonisin B2 ◽  
Dispersive Liquid Liquid Microextraction ◽  
Aflatoxin B2

Consumption of fruit juice is becoming trendy for consumers seeking freshness and high vitamin and low caloric intake. Mycotoxigenic moulds may infect fruits during crop growth, harvest, and storage leading to mycotoxin production. Many mycotoxins are resistant to food processing, which make their presence in the final juice product very likely expected. In this way, the presence of 30 mycotoxins including aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), alternariol (AOH), alternariol monomethyl ether (AME), Ochratoxin A (OTA), fumonisin B1 (FB1), fumonisin B2 (FB2), enniatin A (ENNA), enniatin A1 (ENNA1), enniatin B (ENNB), enniatin B1 (ENNB1), beauvericin (BEA), sterigmatocystin (STG), zearalenone (ZEA), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-ADON), 15-acetyl-deoxynivalenol (15-ADON), diacetoxyscirpenol (DAS), nivalenol (NIV), fusarenon-X (FUS-X), neosolaniol (NEO), patulin (PAT), T-2 toxin and HT-2 toxin was evaluated in 80 juice samples collected from Valencia retail Market. An efficient Dispersive Liquid-Liquid Microextraction method (DLLME) was carried out before their trace level determination by chromatographic techniques coupled to tandem mass spectrometry. The results obtained revealed the presence of nine mycotoxins namely AOH, AME, PAT, OTA, AFB1, AFB2, AFG2, β-ZAL, and HT2 in the analyzed samples, with incidences ranging from 3 to 29% and mean contents between 0.14 and 59.52 µg/L. Considerable percentages of TDIs were reached by children when 200 mL was considered as daily fruit juice intake.

scholarly journals Occurrence of Alternaria and Other Toxins in Cereal Grains Intended for Animal Feeding Collected in Slovenia: A Three-Year Study

Toxins ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 304
Author(s):  
Janja Babič ◽  
Gabrijela Tavčar-Kalcher ◽  
Franci Aco Celar ◽  
Katarina Kos ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Monomethyl Ether ◽  
Cereal Grains ◽  
Agricultural Cooperatives ◽  
Fusarium Toxins ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Alternaria Toxins ◽  
Cereal Grain ◽  
Alternaria Mycotoxins ◽  
Animal Feeding

In recent years, the less-studied Alternaria mycotoxins have attracted increasing interest due to the lack of survey data and their ability to cause toxic effects in animals and humans. To fill the gap, the aim of this three-year survey was to investigate the presence and co-occurrence of Alternaria and other mycotoxins in a total of 433 cereal grain samples from Slovenian farms and agricultural cooperatives from 2014 to 2016. Using the multi-mycotoxin method, 14 mycotoxins were determined. In 53% of 433 analysed samples, contamination with at least one mycotoxin was found. Deoxynivalenol (DON) and tenuazonic acid (TeA) were present in 32% and 26% of cereal grain samples, respectively, whereas alternariol (AOH), tentoxin (TEN), alternariol monomethyl ether (AME), 3- and 15-acetyldeoxynivalenol (3- and 15-AcDON), and zearalenone (ZEN) were present in fewer than 15% of the samples. Ochratoxin A (OTA) was found in one rye sample, while diacetoxyscirpenol (DAS), HT-2 and T-2 toxin, and fumonisins B1 and B2 (FB1 and FB2) were not detected. The highest maximum and median concentrations of Alternaria toxins were determined in spelt in 2016 (TeA, 2277 µg/kg and 203 µg/kg, respectively), and those of Fusarium toxins in wheat in 2015 (DON, 4082 µg/kg and 387 µg/kg, respectively). The co-occurrence of two or more mycotoxins was found in 43% of the positive samples. The correlations between Alternaria toxins were very weak but statistically significant (r: 0.15–0.17, p: 0.0042–0.0165). A well-known correlation between Fusarium toxins DON and ZEN was weak and highly significant (r = 0.28, p < 0.0001).

scholarly journals Associations of eating and drinking habits with cardiovascular disease and diabetes in the adult population: data from the ESSE-RF epidemiological study

2021 ◽  
Vol 20 (5) ◽  
pp. 2982
Author(s):  
N. S. Karamnova ◽  
A. I. Rytova ◽  
O. B. Shvabskaya ◽  
Yu. K. Makarova ◽  
S. A. Maksimov ◽  
...  
Keyword(s):  
Salt Intake ◽  
Eating Habits ◽  
Adult Population ◽  
Daily Intake ◽  
Population Data ◽  
Processed Meat ◽  
Daily Consumption ◽  
Frequency Method ◽  
Animal Fats ◽  
Drinking Habits

The presence of a disease, the prognosis of which can be improved by dietary modification, motivates a patient to change their eating habits.Aim. To study the associations of dietary patterns and alcohol consumption with cardiovascular diseases (CVDs), diabetes, myocardial infarction (MI), and stroke in the adult population.Material and methods. The analysis was carried out using data from representative samples of population from 13 Russian regions aged 2564 years (n=19520; men, 7329; women, 12191). The response rate was ~80%. Dietary characteristics were assessed by frequency method.Results. In the diet of people with CVDs, the daily intake of vegetables/ fruits increases by 84% in men and by 19% in women, while the use of animal fats in cooking decreases by 28% and 20%m respectively (p<0,0001). Women with CVDs reduce the consumption of processed meat and sweets by 16 and 19%, respectively (p<0,005). Persons with prior MI reduce the consumption of sweets in the diet: men by 38% and women by 30%. Men with prior MI have higher daily consumption of cereals by 31%, vegetables and fruits by 46%, low-fat dairy products — 2,4 times. In addition, they are more adherent to a healthy and cardioprotective diet by 3,65 and 1,75 times, respectively. Dietary changes in those with prior stroke were noted only in women in the form of a 29% decrease in excess salt intake (p=0,0075). In the diet of people with diabetes, there is decreased consumption of sweets and an increased intake of vegetables/fruits: by 77 and 69% in men and by 79 and 69% in women, respectively (p<0,0001). Men with diabetes are 3 times more likely to adhere to a healthy diet, and women — 2,3 times (p=0,0039 and p<0,0001, respectively).Conclusion. Patients with CVDs, MI, and diabetes have a healthier diet than healthy persons.

scholarly journals Dietary Exposure of Thais to Pesticides During 1989–1996

2002 ◽  
Vol 85 (1) ◽  
pp. 134-140 ◽  
Author(s):  
Amara Vongbuddhapitak ◽  
Kanokporn Atisook ◽  
Gobthong Thoophom ◽  
Boonpai Sungwaranond ◽  
Yuwadee Lertreungdej ◽  
...  
Keyword(s):  
Food Consumption ◽  
Analytical Methods ◽  
Pesticide Residues ◽  
Daily Intake ◽  
Dietary Exposure ◽  
Dietary Intakes ◽  
Daily Consumption ◽  
Department Of Health ◽  
Food Consumption Survey ◽  
Consumption Survey

Abstract To monitor the exposure of the Thai to pesticide residues in foods, the intake of pesticides based on the total diet approach has been studied in Thailand continually since 1989. Food items were chosen and their proportions of daily consumption were calculated from the national food consumption survey conducted by the Department of Health in 1986. Seventy-seven items of food including drinking water were classified into 12 groups according to their sources of contamination and the analytical methods used. Shopping lists and standard procedures for collecting samples and cooking were developed. Twelve food composites from each of the 4 regions were analyzed for nearly 100 pesticides. All analyses used multiresidue analytical methods, and the total daily dietary intakes of pesticide residues were estimated from the average amount of food consumed by Thais. Among 24 pesticides found in the 8 year study, DDT, dimethoate, methamidophos, and parathion methyl were found every year. However, dietary intakes of all pesticides were far below the established acceptable daily intake. In 1999, the study methodology was redesigned to accommodate the new national food consumption survey data.

scholarly journals Alternaria toxins alternariol and alternariol monomethyl ether in grain foods in Canada

2012 ◽  
Vol 28 (4) ◽  
pp. 261-266 ◽  
Author(s):  
Peter M. Scott ◽  
Wendy Zhao ◽  
Sherry Feng ◽  
Benjamin P.-Y. Lau
Keyword(s):  
Monomethyl Ether ◽  
Alternariol Monomethyl Ether ◽  
Alternaria Toxins ◽  
Grain Foods

Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs

2008 ◽  
Vol 1 (2) ◽  
pp. 175-188 ◽  
Author(s):  
V. Ostry
Keyword(s):  
Topoisomerase I ◽  
Edible Oils ◽  
Solid Phase ◽  
Chemical Characterization ◽  
Monomethyl Ether ◽  
Dna Integrity ◽  
Natural Occurrence ◽  
Tenuazonic Acid ◽  
Alternariol Monomethyl Ether ◽  
Alternaria Mycotoxins

Microfungi of the genus Alternaria are ubiquitous pathogens and saprophytes. Many species of the genus Alternaria commonly cause spoilage of various food crops in the field or post-harvest decay. Due to their growth even at low temperatures, they are also responsible for spoilage of these commodities during refrigerated transport and storage. Several Alternaria species are known producers of toxic secondary metabolites - Alternaria mycotoxins. A. alternata produces a number of mycotoxins, including alternariol, alternariol monomethyl ether, altenuene, altertoxins I, II, III, tenuazonic acid and other less toxic metabolites. Tenuazonic acid is toxic to several animal species, e.g. mice, chicken, dogs. Alternariol, alternariol monomethyl ether, altenuene and altertoxin I are not very acutely toxic. There are several reports on the mutagenicity and genotoxicity of alternariol, and alternariol monomethyl ether. Alternariol has been identified as a topoisomerase I and II poison which might contribute to the impairment of DNA integrity in human colon carcinoma cells. Analytical methods to determine Alternaria toxins are largely based on procedures, involving cleanup by solvent partitioning or solid phase extraction, followed by chromatographic separation techniques, in combination with ultraviolet, fluorescence, electrochemical and mass spectroscopic detection. A large number of Alternaria metabolites has been reported to occur naturally in food commodities (e.g. fruit, vegetables, cereals and oil plants). Alternariol, alternariol monomethyl ether and tenuazonic acid were frequently detected in apples, apple products, mandarins, olives, pepper, red pepper, tomatoes, tomato products, oilseed rape meal, sunflower seeds, sorghum, wheat and edible oils. Alternariol and alternariol monomethyl ether were detected in citrus fruit, Japanese pears, prune nectar, raspberries, red currant, carrots, barley and oats. Alternariol monomethyl ether and tenuazonic acid were detected in melon. Natural occurrence of alternariol has been reported in apple juice, cranberry juice, grape juice, prune nectar, raspberry juice, red wine and lentils.

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