Cricket powder as an alternative protein source for broilers based on in vitro digestibility

2019 ◽  
Vol 5 (3) ◽  
pp. 185-191 ◽  
Author(s):  
A. Kovitvadhi ◽  
P. Chundang ◽  
J. Luapan ◽  
P. Amarapitak ◽  
P. Sriyaphai ◽  
...  
Keyword(s):  
Crude Protein ◽  
Duodenal Mucosa ◽  
Acheta Domesticus ◽  
Protein Source ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Crude Enzyme Extract ◽  
Alternative Protein ◽  
Nutrient Utilisation

Pathogen contamination in fishmeal (FM) and the imbalanced amino acid profile in soya meal (SYM) are the disadvantages of the common protein sources in broiler diets. Therefore, FM, SYM and three species of cricket powder (Acheta domesticus: AD, Gryllus testaceus: GT and Gryllus bimaculatus: GB) were screened based on the in vitro digestibility of dry matter (DMd), organic matter (OMd) and crude protein (CPd) to assess whether these insects could be a sustainable alternative protein source for broiler nutrition. Stomach mucosa, duodenal mucosa and pancreas were extracted from broilers to obtain crude enzyme extract (CTX) using two stages of in vitro digestibility. The first stage was performed using gastric mucosa CTX, whereas CTX from the duodenal mucosa and pancreas were added for the second stage. SYM and GB had similar DMd, which were lower than FM, AD and GT (P<0.01). The highest OMd was observed in FM, followed by AD, GT, GB and SYM (P<0.01). A higher CPd was found in the crude protein calibration group (CPd-Cal), in which substrates were adjusted to obtain an equivalent crude protein level as the non-calibration group (CPd-Non). Interestingly, a strong correlation (r=0.99, P<0.01) was found between CPd-Cal and CPd-Non, with the highest CPd presented in FM, followed by SYM, AD, GB and GT (P<0.01). A significant negative correlation was found between the CPd from both groups (CPd-Cal and CPd-Non) and crude fibre (r=-0.96 and -0.95, P<0.05, respectively), whereas a positive correlation was observed for ash (r=0.91 and 0.93, P<0.05, respectively). AD has great potential as a protein source for broilers, greater than other cricket species, based on its DMd, OMd and CPd. However, the CPd of all insect species was lower than that of FM and SYM. Therefore, pre-treatment protocols should be performed to improve nutrient utilisation.

scholarly journals High Fiber Cakes from Mediterranean Multipurpose Oilseeds as Protein Sources for Ruminants

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 918 ◽  
Author(s):  
Francesco Serrapica ◽  
Felicia Masucci ◽  
Emiliano Raffrenato ◽  
Maura Sannino ◽  
Alessandro Vastolo ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Ether Extract ◽  
Protein Supplements ◽  
High Fiber ◽  
Alternative Protein

Fifteen oilseed cakes from sunflower, pomegranate, cardoon, tobacco and hemp were characterized with regard to chemical composition, Cornell Net Carbohydrate and Protein System (CNCPS) fractionation, in vitro digestibility of dry matter, neutral detergent fiber, and crude protein. All the cakes presented low moisture, rather variable ether extract contents and medium to high levels of crude protein and neutral detergent fiber. The cakes significantly differed in terms of CNCPS partitioning and in vitro digestibility. Tobacco and hemp cakes presented high contents of slow degradable fractions of crude protein and carbohydrate joined to good post-ruminal protein digestibility. Cardoon cakes presented the highest rumen protein degradability. Based on crude protein content and intestinal digestibility of rumen undegraded protein, cakes of tobacco and hemp showed the better potential as alternative protein supplements for ruminants, while pomegranate appears to be the least suitable for ruminant feeding.

scholarly journals Extraction of leaf protein from green crops. Chemical composition and nutritive value of products of fractionation 

1983 ◽  
Vol 55 (2) ◽  
pp. 143-154
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Soluble Sugars ◽  
Protein Solubility ◽  
Leaf Protein ◽  
In Vitro Digestibility ◽  
Growth Stages ◽  
Crude Fibre ◽  
True Protein

Leaf protein was extracted from different green crops in 11 pilot plant esperiments. Of the crops, 4 were grass, 6 clover and one pea. The extraction of juice was on average 55 % of the fresh weight of the green crop and the values for dry matter (DM) and crude protein (CP) were 22.6 and 24.1 %. Clover gave better recoveries of protein than grass. In the leaf protein concentrate (LPC) obtained from the juice, the separation ratios for DM, CP and TP (true protein) were, respectively, 23.7 %, 48.0 and 80.7 %. Heating to 85°C gave more efficient recoveries of LPC than the combination of heating and acid precipitation. The average DM content of the pressed pulp was 30.4 %, the corresponding value for the whole crop being 16.5 %. Measured on a DM basis, the CP content of the pressed pulp was only 0.4 % units lower than in untreated forage, but the crude fibre content was 7.3 % units higher. In vitro organic matter digestibility and the pepsin-HCI solubility of crude protein were on average 5.1 and 5.5 % units lower in the pulp. The average DM of the plant juice was 6.5 % and contained 21.9 % ash, 21.5 % CP, 10.7 % TP and 29.9 % soluble sugars. Clover and pea had much higher values for CP and TP than grass. In the LPC preparations, CP and TP averaged 43.6 % and 38.5 % of DM. Heat treatment gave higher protein content than precipitation of LPC by combined heating and acidification. The in vitro digestibility and protein solubility of LPC were high, on average 85.6 % and 80.2 %. LPC had fairly high contents of lysine and methionine, 4.1 % and 1.6 g/16 g N. There were only small differences in the amino acid composition between grass and clover and between crops harvested at different growth stages. Green crop fractionation is a potential means of improving grassland production and utilization. Promising results have been obtained with plant juice and LPC fed to monogastric animals and pressed pulp residues in diets for ruminants. The economic aspects of fractionation remain to be evaluated.

Laboratory analyses of the nutritional value of western Queensland browse feeds

1979 ◽  
Vol 19 (98) ◽  
pp. 344 ◽  
Author(s):  
WJF McDonald ◽  
JH Ternouth
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Nitrogen Free Extract ◽  
Organic Matter Digestibility

Seventy samples of browse feeds, collected during late autumn and spring of 1971 from 42 western Queensland shrubs and trees, were subjected to laboratory analyses of the proximate fractions and in vitro digestibility. Crude protein, crude fibre and nitrogen-free-extract fractions ranged between 65 and 242, 55 and 353 and 425 and 71 1 g kg-1 dry matter, repectively. The in vitro dry matter digestibility coefficients ranged between 0.22 and 0.86. Comparisons of 15 browse feeds collected in April-May and October indicated a higher crude protein content (P < 0.05) and in vitro digestibility (P .= 0.01) and lower crude fibre (P < 0.01) and ash (P < 0.01 ) content in spring samples for most species. Nine browse feeds of known in vivo dry matter and organic matter digestibility were subjected to in vitro digestibility analysis. Whilst the in vitro and in vivo dry matter digestibilities were similar (0.43 and 0.45, respectively) the mean in vitro organic matter digestibility was substantially lower than the corresponding in vivo digestibility (0.40 and 0.46, respectively).

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

scholarly journals Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales

2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Cuk Tri Noviandi ◽  
Dibya Ratnopama ◽  
Ali Agus ◽  
Ristianto Utomo
Keyword(s):  
Rice Straw ◽  
Crude Protein ◽  
Production Method ◽  
Proximate Analysis ◽  
Gas Production ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Nutrient Quality ◽  
Randomized Design

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.

YIELD AND QUALITY OF SOME KALE-CORN COMBINATIONS

1980 ◽  
Vol 60 (3) ◽  
pp. 807-811 ◽  
Author(s):  
R. S. FULKERSON
Keyword(s):  
Protein Content ◽  
Corn Stover ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Protein Content ◽  
Yield And Quality ◽  
Row Width

Midas marrowstem kale (Brassica oleracea L.) was grown in different row width associations with United 106 corn (Zea maize L.) in two studies and ensiled in different moisture blends with corn stover in another. Highest dry matter yields were obtained where a single row of kale was grown at 30 cm to the side of a corn row. This combination also provided the lowest moisture content feed and the highest in vitro digestibility and crude protein content. Changing the corn row width had no significant effect upon yield, plant height, in vitro digestibility, kale leaf or corn ear content. Blending kale with corn stover to provide a silage of about 70% moisture increased the digestibility and protein content of the feed and provided a silage that kept well in storage.

CHEMICAL COMPOSITION AND IN VITRO DIGESTIBILITY OF RANGE FORAGE PLANTS OF THE STIPA-BOUTELOUA PRAIRIE

1967 ◽  
Vol 47 (2) ◽  
pp. 161-167 ◽  
Author(s):  
S. Smoliak ◽  
L. M. Bezeau
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Calcium Content ◽  
Early Spring ◽  
In Vitro Digestibility ◽  
Native Grasses ◽  
Stages Of Growth ◽  
Artemisia Species ◽  
Shrubby Species

Five native grasses and one sedge of the Stipa-Bouteloua prairie, three introduced grasses collected at five stages of growth, and four shrubby species collected at three stages of growth were analyzed for proximate chemical constituents.The amounts of phosphorus and digestible and crude protein of all species decreased with maturity, while the cellulose increased. Introduced grasses generally contained more crude protein than native grasses. Shrubby species were higher in crude protein and phosphorus than native grasses. No consistent trend was observed in relative total ash and calcium content at progressive stages of development. The estimated nutritive value index was high for all grasses and low for two Artemisia species. The seasonal declines in crude protein and phosphorus suggest that protein and phosphorus supplements are desirable for range cattle during the fall, winter, and early spring.

In situ degradability of organic matter, crude protein and cell wall of various tree forages

1992 ◽  
Vol 55 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Kamatali ◽  
E. Teller ◽  
M. Vanbelle ◽  
G. Collignon ◽  
M. Foulon
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Crude Protein ◽  
In Vitro Digestibility ◽  
Sesbania Sesban ◽  
Holstein Friesian ◽  
Ruminal Degradation

AbstractLeaves of Leucaena leucocephala, Sesbania sesban and Calliandra callothyrsus were harvested in Rwanda from young shoots at 8 weeks after the first cutting. They were dried, ground and incubated at the same time in polyester bags in three non-lactating Holstein-Friesian cows each fitted with a ruminal cannula. The bags were removed at 0, 2, 4, 8, 24, 48, 72 and 144 h after the start of incubations. The different parameters characterizing extent and rate of ruminal degradation of organic matter (OM), neutral-detergent fibre (NDF), and crude protein (CP) were calculated. In vitro digestibility of residual protein after 24 h and 48 h incubation was also determined. Sesbania sesban had lowest cell wall contents and gave highest ruminal degradability for OM, NDF and CP. Leucaena leucocephala was degraded to a lesser extent, but its undegraded protein had a somewhat higher in vitro digestibility. In contrast, protein of Calliandra callothyrsus was poorly degraded and digested. The proportion and composition of cell wall could not explain these differences in digestion characteristics and other measurements, such as tannins, were incriminated. Increased ruminal incubation time augmented the extent of ruminal degradation and reduced in vitro digestibility of undegraded protein but did not affect the undigestible protein fraction.

Selection for crude protein content in Phalaris tuberosa L. I. Response to selection and preliminary studies on correlated responses

1969 ◽  
Vol 20 (4) ◽  
pp. 643 ◽  
Author(s):  
RJ Clements
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Breeding Population ◽  
In Vitro Digestibility ◽  
Seedling Vigour ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Selection For ◽  
Pasture Plant

In a highly variable breeding population of P. tuberosa, marked responses were obtained to three generations of selection for high and low crude protein concentration (percentage nitrogen x 6.25) in whole tillers at heading. Total response was similar in each direction, and realized heritability estimates were h2 = 0.25 and h2 = 0.20 in the high and low directions respectively. The responses were accompanied by positively correlated changes in in vitro digestibility and in characters commonly used as indicators of nutritive value of herbage. However, there were large negatively correlated changes in seedling vigour, relative growth rates, and other morphological and physiological characters. The implications of the results for pasture plant breeding are discussed.

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