scholarly journals Inhibition of CD73 stimulates the migration and invasion of B16F10 melanoma cells in vitro, but results in impaired angiogenesis and reduced melanoma growth in vivo

2013 ◽  
Vol 31 (2) ◽  
pp. 819-827 ◽  
Author(s):  
PATRYCJA KOSZAŁKA ◽  
ANNA PRYSZLAK ◽  
MONIKA GOŁUŃSKA ◽  
JUSTYNA KOLASA ◽  
GRZEGORZ STASIŁOJĆ ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Migration And Invasion ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Impaired Angiogenesis ◽  
Melanoma Growth

Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study

2018 ◽  
Vol 283 ◽  
pp. 10-19 ◽  
Author(s):  
Aïcha Sassi ◽  
Mouna Maatouk ◽  
Dorra El gueder ◽  
Imen Mokdad Bzéouich ◽  
Saïda Abdelkefi-Ben Hatira ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Melanoma Cells ◽  
Biologically Active ◽  
In Vivo Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Antimelanogenic activities of piperlongumine derived from Piper longum on murine B16F10 melanoma cells in vitro and zebrafish embryos in vivo: its molecular mode of depigmenting action

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Hwang-Ju Jeon ◽  
Kyeongnam Kim ◽  
Yong-Deuk Kim ◽  
Sung-Eun Lee
Keyword(s):  
Melanoma Cells ◽  
Positive Control ◽  
Zebrafish Embryos ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Pharmaceutical Industries

Abstract In this study, the antimelanogenic activity of piperlongumine in murine B16F10 melanoma cells and zebrafish was investigated, and its mode of antimelanogenic action was elucidated using quantitative reverse transcription-polymerase chain reaction. A melanocyte-stimulating hormone (α-MSH, 200 nM) was used to induce melanin production in B16F10 melanoma cells, and kojic acid (200 μM) was used as a positive control. Piperlongumine had no inhibitory effects on cell growth at the treated concentrations (3 and 6 μM), and it significantly reduced total melanin production. Piperlongumine decreased the expression of Mitf, Tyr, Trp-1, and Trp-2 and tyrosinase activity was also dramatically reduced by the piper amide addition under α-MSH treatment. With these findings, zebrafish embryos were used to confirm antimelanogenic activity of piperlongumine, and it showed the potent antimelanogenic activity at the concentration of 1 μM. Altogether, piperlongumine has potent antimelanogenic activity, and these results support it as a candidate for natural depigmentation agent in a cosmetic and pharmaceutical industries.

scholarly journals The Effect of Bovine Cartilage on the Growth of Mouse B16F10 Melanoma Cells in vitro and in vivo

2016 ◽  
Vol 12 (3) ◽  
pp. 69-76
Author(s):  
Arax Tanelian ◽  
Dalal F. Jaber ◽  
Nayla S. Al Akl ◽  
Alexander M. Abdelnoor
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Bovine Cartilage

In vitro and in vivo growth of B16F10 melanoma cells transfected with interleukin-4 cDNA and gene therapy with the transfectant

1994 ◽  
Vol 120 (11) ◽  
pp. 631-635 ◽  
Author(s):  
Tatsuo Ohira ◽  
Yuichiro Ohe ◽  
Yuji Heike ◽  
Eckhard R. Podack ◽  
Kristin J. Olsen ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Melanoma Cells ◽  
Interleukin 4 ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
In Vivo Growth

Effect of corchorusin-D, a saikosaponin like compound, on B16F10 melanoma cells (in vitro and in vivo)

2013 ◽  
Vol 15 (11) ◽  
pp. 1197-1203 ◽  
Author(s):  
Sumana Mallick ◽  
Bikas Chandra Pal ◽  
Deepak Kumar ◽  
Nabanita Chatterjee ◽  
Subhadip Das ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

An In-Vitro Assay Estimating Changes in Melanin Content of Melanoma Cells due to Ultra-Dilute, Potentized Preparations

Homeopathy ◽  
2019 ◽  
Vol 108 (03) ◽  
pp. 183-187 ◽  
Author(s):  
Renuka Munshi ◽  
Samidha Joshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16f10 Melanoma ◽  
Vitro Assay ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Physiological Dose

Introduction The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (H2O2; HP), 6-biopterin (BP), and [Nle4, D-Phe7]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose. Materials and Methods The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined. Results At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content (p = 0.015 and p = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity. Conclusion The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.

Pharmacological Inhibition of USP7 Supresses Growth and Metastasis of Melanoma Cells in Vitro and in Vivo

2020 ◽  
Author(s):  
Minmin Xiang ◽  
Long Liang ◽  
Xinwei Kuang ◽  
Zuozhong Xie ◽  
Jing Liu ◽  
...  
Keyword(s):  
Scratch Test ◽  
Resistance Mechanisms ◽  
Melanoma Cells ◽  
Migration And Invasion ◽  
Western Blot Assay ◽  
Novel Therapeutic Strategies ◽  
Novel Therapeutic

Abstract Background: Melanoma is a highly aggressive type of skin cancer. Due to the development of diverse resistance mechanisms and severe adverse side effects, significantly limits the efficiency of current therapeutic approaches. Identification of the new therapeutic targets involved in the pathogenesis will benefit to develop novel therapeutic strategies. The deubiquitinase USP7(ubiquitin-specific protease-7) is deregulated in serval cancer types, as a potential target for cancer treatment, but its role in melanoma is still unclear. Here, we investigated the role of USP7 and its inhibitor P22077 in melanoma treatment.Methods: To explore the role of USP7 and the anti-tuomr effect of P22077 in melanma progression and metastasis, a series of cell biological, molecular and biochemical approaches were used for in vitro and in vivo investigations.These methods included RT-qPCR, Western blot assay, cell transfection, CCK8 assay, flow cytometry, scratch test, Transwell assay, mouse xenograft,TUNEL staining.Results:The USP7 inhibitor P22077 suppressed the growth of melanoma in vitro and in vivo. additionally, P22077 induction of cell cycle arrest and apoptosis via ROS(reactive oxygen species) accumulation-induced DNA damage. Furthermore, inhibition of USP7 also prevented migration and invasion of melanoma cells in vitro and in vivo by decrease the Wnt/β-catenin signal pathway. Conclusion: Our data indicated that USP7 acts as an oncogene involved in melanoma cell proliferation and metastasis and may provide a novel therapeutic target for melanoma treatment.

scholarly journals INCREASED LEVEL OF MIR-204-5P EXPRESSION IN MELANOMA CELLS UNDER THE INFLUENCE OF DACARBAZINE

2019 ◽  
Vol 18 (3) ◽  
pp. 45-53
Author(s):  
S. N. Lavrentiev ◽  
M. B. Aksenenko ◽  
A. S. Averchuk ◽  
A. V. Komina ◽  
N. V. Palkina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Animal Behavior ◽  
Growth Dynamics ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Migration And Invasion ◽  
Antitumor Effects ◽  
Metastasis Development

Various types of tissues was analyzed, and the algorithm for summing neutron and photon doses in neutronMiRNA s are involved in the regulation of numerous critical biological processes, including cell proliferation, differentiation, migration and invasion. They function as oncogenes or tumor suppressors according to the nature of the target. It has been previously determined that miR-204-5p miRNA is characterized by the increased level in melanoma. The aim of this study was to determine the effects of changes in the level of microRNA expression when dacarbazine was exposed to melanoma cells in vitro and synthetic miR-204-5p in vivo. The expression levels of miR-204-5p and miR-211 in melanoma cells were determined by real-time PCR. Antitumor effects in vivo were verified in assessing the growth dynamics of the tumor node. Toxic effects were assessed by animal behavior, fluid intake, feed, and ALT , AST , creatinine, urea levels. In the model of melanoma C57BL6, it was revealed that the introduction of the synthetic miR-204-5p did not cause significant changes in the investigated microRNA in tumor cells. At the same time, the antitumor effects of dacarbazine in melanoma cells in vitro led to an increase in the level of the investigated microRNA by more than 20 times. The results of the study indicated the possibility of compensating the level of miR-204-5p under the influence of cytostatic therapy. Taking into account the previously revealed miR-204-5p inhibitory effect on the proliferation of melanoma cells, we can assume that this miRNA can play a role in maintaining the dermal state of tumor cells. Further studies are required to understand the metastasis development and predict the response to antitumor therapy for melanoma.

scholarly journals Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study

2014 ◽  
Author(s):  
Anderson F. Miranda ◽  
Gustavo M. P. Santos ◽  
Susana C. P. S. de Oliveira ◽  
Juliana S. C. Monteiro ◽  
Fernando J. P. Sampaio ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Vitro Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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