scholarly journals Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study

2014 ◽  
Author(s):  
Anderson F. Miranda ◽  
Gustavo M. P. Santos ◽  
Susana C. P. S. de Oliveira ◽  
Juliana S. C. Monteiro ◽  
Fernando J. P. Sampaio ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Vitro Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

An In-Vitro Assay Estimating Changes in Melanin Content of Melanoma Cells due to Ultra-Dilute, Potentized Preparations

Homeopathy ◽  
2019 ◽  
Vol 108 (03) ◽  
pp. 183-187 ◽  
Author(s):  
Renuka Munshi ◽  
Samidha Joshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16f10 Melanoma ◽  
Vitro Assay ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Physiological Dose

Introduction The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (H2O2; HP), 6-biopterin (BP), and [Nle4, D-Phe7]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose. Materials and Methods The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined. Results At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content (p = 0.015 and p = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity. Conclusion The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.

Effect of targeted gold nanoparticles size on acoustic cavitation: An in vitro study on melanoma cells

Ultrasonics ◽  
2020 ◽  
Vol 102 ◽  
pp. 106061 ◽  
Author(s):  
Ahmad Shanei ◽  
Hadi Akbari-Zadeh ◽  
Neda Attaran ◽  
Mohammad Reza Salamat ◽  
Milad Baradaran-Ghahfarokhi
Keyword(s):  
Gold Nanoparticles ◽  
In Vitro Study ◽  
Acoustic Cavitation ◽  
Melanoma Cells ◽  
Vitro Study

Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study

2018 ◽  
Vol 283 ◽  
pp. 10-19 ◽  
Author(s):  
Aïcha Sassi ◽  
Mouna Maatouk ◽  
Dorra El gueder ◽  
Imen Mokdad Bzéouich ◽  
Saïda Abdelkefi-Ben Hatira ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Melanoma Cells ◽  
Biologically Active ◽  
In Vivo Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

The role of MITF and Mcl-1 proteins in the antiproliferative and proapoptotic effect of ciprofloxacin in amelanotic melanoma cells: In silico and in vitro study

2020 ◽  
Vol 66 ◽  
pp. 104884 ◽  
Author(s):  
Artur Beberok ◽  
Jakub Rok ◽  
Zuzanna Rzepka ◽  
Krzysztof Marciniec ◽  
Stanisław Boryczka ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Study ◽  
Melanoma Cells ◽  
Amelanotic Melanoma ◽  
Vitro Study

Cytotoxic and proapoptotic effect of doxycycline – An in vitro study on the human skin melanoma cells

2020 ◽  
Vol 65 ◽  
pp. 104790 ◽  
Author(s):  
Jakub Rok ◽  
Marta Karkoszka ◽  
Zuzanna Rzepka ◽  
Michalina Respondek ◽  
Klaudia Banach ◽  
...  
Keyword(s):  
Human Skin ◽  
In Vitro Study ◽  
Melanoma Cells ◽  
Vitro Study ◽  
Skin Melanoma

scholarly journals GSK-3β-Targeting Fisetin Promotes Melanogenesis in B16F10 Melanoma Cells and Zebrafish Larvae through β-Catenin Activation

2020 ◽  
Vol 21 (1) ◽  
pp. 312 ◽  
Author(s):  
Ilandarage Menu Neelaka Molagoda ◽  
Wisurumuni Arachchilage Hasitha Maduranga Karunarathne ◽  
Sang Rul Park ◽  
Yung Hyun Choi ◽  
Eui Kyun Park ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Glycogen Synthase ◽  
Melanoma Cells ◽  
Functional Assay ◽  
Mushroom Tyrosinase ◽  
B16f10 Melanoma ◽  
Zebrafish Larvae ◽  
B16f10 Melanoma Cells ◽  
Gsk 3Β

Fisetin is found in many fruits and plants such as grapes and onions, and exerts anti-inflammatory, anti-proliferative, and anticancer activity. However, whether fisetin regulates melanogenesis has been rarely studied. Therefore, we evaluated the effects of fisetin on melanogenesis in B16F10 melanoma cell and zebrafish larvae. The current study revealed that fisetin slightly suppressed in vitro mushroom tyrosinase activity; however, molecular docking data showed that fisetin did not directly bind to mushroom tyrosinase. Unexpectedly, fisetin significantly increased intracellular and extracellular melanin production in B16F10 melanoma cells regardless of the presence or absence of α-melanocyte stimulating hormone (α-MSH). We also found that the expression of melanogenesis-related genes such as tyrosinase and microphthalmia-associated transcription factor (MITF), were highly increased 48 h after fisetin treatment. Pigmentation of zebrafish larvae by fisetin treatment also increased at the concentrations up to 200 µM and then slightly decreased at 400 µM, with no alteration in the heart rates. Molecular docking data also revealed that fisetin binds to glycogen synthase kinase-3β (GSK-3β). Therefore, we evaluated whether fisetin negatively regulated GSK-3β, which subsequently activates β-catenin, resulting in melanogenesis. As expected, fisetin increased the expression of β-catenin, which was subsequently translocated into the nucleus. In the functional assay, FH535, a Wnt/β-catenin inhibitor, significantly inhibited fisetin-mediated melanogenesis in zebrafish larvae. Our data suggested that fisetin inhibits GSK-3β, which activates β-catenin, resulting in melanogenesis through the revitalization of MITF and tyrosinase.

scholarly journals Antimelanogenic activities of piperlongumine derived from Piper longum on murine B16F10 melanoma cells in vitro and zebrafish embryos in vivo: its molecular mode of depigmenting action

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Hwang-Ju Jeon ◽  
Kyeongnam Kim ◽  
Yong-Deuk Kim ◽  
Sung-Eun Lee
Keyword(s):  
Melanoma Cells ◽  
Positive Control ◽  
Zebrafish Embryos ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Pharmaceutical Industries

Abstract In this study, the antimelanogenic activity of piperlongumine in murine B16F10 melanoma cells and zebrafish was investigated, and its mode of antimelanogenic action was elucidated using quantitative reverse transcription-polymerase chain reaction. A melanocyte-stimulating hormone (α-MSH, 200 nM) was used to induce melanin production in B16F10 melanoma cells, and kojic acid (200 μM) was used as a positive control. Piperlongumine had no inhibitory effects on cell growth at the treated concentrations (3 and 6 μM), and it significantly reduced total melanin production. Piperlongumine decreased the expression of Mitf, Tyr, Trp-1, and Trp-2 and tyrosinase activity was also dramatically reduced by the piper amide addition under α-MSH treatment. With these findings, zebrafish embryos were used to confirm antimelanogenic activity of piperlongumine, and it showed the potent antimelanogenic activity at the concentration of 1 μM. Altogether, piperlongumine has potent antimelanogenic activity, and these results support it as a candidate for natural depigmentation agent in a cosmetic and pharmaceutical industries.

scholarly journals The Effect of Bovine Cartilage on the Growth of Mouse B16F10 Melanoma Cells in vitro and in vivo

2016 ◽  
Vol 12 (3) ◽  
pp. 69-76
Author(s):  
Arax Tanelian ◽  
Dalal F. Jaber ◽  
Nayla S. Al Akl ◽  
Alexander M. Abdelnoor
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Bovine Cartilage
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