scholarly journals The Effect of Bovine Cartilage on the Growth of Mouse B16F10 Melanoma Cells in vitro and in vivo

2016 ◽  
Vol 12 (3) ◽  
pp. 69-76
Author(s):  
Arax Tanelian ◽  
Dalal F. Jaber ◽  
Nayla S. Al Akl ◽  
Alexander M. Abdelnoor
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Bovine Cartilage

Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study

2018 ◽  
Vol 283 ◽  
pp. 10-19 ◽  
Author(s):  
Aïcha Sassi ◽  
Mouna Maatouk ◽  
Dorra El gueder ◽  
Imen Mokdad Bzéouich ◽  
Saïda Abdelkefi-Ben Hatira ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Melanoma Cells ◽  
Biologically Active ◽  
In Vivo Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Antimelanogenic activities of piperlongumine derived from Piper longum on murine B16F10 melanoma cells in vitro and zebrafish embryos in vivo: its molecular mode of depigmenting action

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Hwang-Ju Jeon ◽  
Kyeongnam Kim ◽  
Yong-Deuk Kim ◽  
Sung-Eun Lee
Keyword(s):  
Melanoma Cells ◽  
Positive Control ◽  
Zebrafish Embryos ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Pharmaceutical Industries

Abstract In this study, the antimelanogenic activity of piperlongumine in murine B16F10 melanoma cells and zebrafish was investigated, and its mode of antimelanogenic action was elucidated using quantitative reverse transcription-polymerase chain reaction. A melanocyte-stimulating hormone (α-MSH, 200 nM) was used to induce melanin production in B16F10 melanoma cells, and kojic acid (200 μM) was used as a positive control. Piperlongumine had no inhibitory effects on cell growth at the treated concentrations (3 and 6 μM), and it significantly reduced total melanin production. Piperlongumine decreased the expression of Mitf, Tyr, Trp-1, and Trp-2 and tyrosinase activity was also dramatically reduced by the piper amide addition under α-MSH treatment. With these findings, zebrafish embryos were used to confirm antimelanogenic activity of piperlongumine, and it showed the potent antimelanogenic activity at the concentration of 1 μM. Altogether, piperlongumine has potent antimelanogenic activity, and these results support it as a candidate for natural depigmentation agent in a cosmetic and pharmaceutical industries.

scholarly journals Inhibition of CD73 stimulates the migration and invasion of B16F10 melanoma cells in vitro, but results in impaired angiogenesis and reduced melanoma growth in vivo

2013 ◽  
Vol 31 (2) ◽  
pp. 819-827 ◽  
Author(s):  
PATRYCJA KOSZAŁKA ◽  
ANNA PRYSZLAK ◽  
MONIKA GOŁUŃSKA ◽  
JUSTYNA KOLASA ◽  
GRZEGORZ STASIŁOJĆ ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Migration And Invasion ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Impaired Angiogenesis ◽  
Melanoma Growth

In vitro and in vivo growth of B16F10 melanoma cells transfected with interleukin-4 cDNA and gene therapy with the transfectant

1994 ◽  
Vol 120 (11) ◽  
pp. 631-635 ◽  
Author(s):  
Tatsuo Ohira ◽  
Yuichiro Ohe ◽  
Yuji Heike ◽  
Eckhard R. Podack ◽  
Kristin J. Olsen ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Melanoma Cells ◽  
Interleukin 4 ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
In Vivo Growth

Effect of corchorusin-D, a saikosaponin like compound, on B16F10 melanoma cells (in vitro and in vivo)

2013 ◽  
Vol 15 (11) ◽  
pp. 1197-1203 ◽  
Author(s):  
Sumana Mallick ◽  
Bikas Chandra Pal ◽  
Deepak Kumar ◽  
Nabanita Chatterjee ◽  
Subhadip Das ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

An In-Vitro Assay Estimating Changes in Melanin Content of Melanoma Cells due to Ultra-Dilute, Potentized Preparations

Homeopathy ◽  
2019 ◽  
Vol 108 (03) ◽  
pp. 183-187 ◽  
Author(s):  
Renuka Munshi ◽  
Samidha Joshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16f10 Melanoma ◽  
Vitro Assay ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Physiological Dose

Introduction The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (H2O2; HP), 6-biopterin (BP), and [Nle4, D-Phe7]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose. Materials and Methods The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined. Results At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content (p = 0.015 and p = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity. Conclusion The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.

scholarly journals Phenothiazinium dyes in association with diode red laser against B16F10 melanoma cells: in vitro study

2014 ◽  
Author(s):  
Anderson F. Miranda ◽  
Gustavo M. P. Santos ◽  
Susana C. P. S. de Oliveira ◽  
Juliana S. C. Monteiro ◽  
Fernando J. P. Sampaio ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Vitro Study ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Involvement of Mitochondrial Mechanisms in the Cytostatic Effect of Desethylamiodarone in B16F10 Melanoma Cells

2020 ◽  
Vol 21 (19) ◽  
pp. 7346
Author(s):  
Fadi H. J. Ramadan ◽  
Aliz Szabo ◽  
Dominika Kovacs ◽  
Aniko Takatsy ◽  
Rita Bognar ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescent Dyes ◽  
Mitochondrial Permeability Transition ◽  
Imaging System ◽  
Coupling Efficiency ◽  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
Atp Production ◽  
B16f10 Melanoma Cells

Previously, we showed that desethylamiodarone (DEA), a major metabolite of the widely used antiarrhythmic drug amiodarone, has direct mitochondrial effects. We hypothesized that these effects account for its observed cytotoxic properties and ability to limit in vivo metastasis. Accordingly, we examined DEA’s rapid (3–12 h) cytotoxicity and its early (3–6 h) effects on various mitochondrial processes in B16F10 melanoma cells. DEA did not affect cellular oxygen radical formation, as determined using two fluorescent dyes. However, it did decrease the mitochondrial transmembrane potential, as assessed by JC-1 dye and fluorescence microscopy. It also induced mitochondrial fragmentation, as visualized by confocal fluorescence microscopy. DEA decreased maximal respiration, ATP production, coupling efficiency, glycolysis, and non-mitochondrial oxygen consumption measured by a Seahorse cellular energy metabolism analyzer. In addition, it induced a cyclosporine A–independent mitochondrial permeability transition, as determined by Co2+-mediated calcein fluorescence quenching measured using a high-content imaging system. DEA also caused outer mitochondrial membrane permeabilization, as assessed by the immunoblot analysis of cytochrome C, apoptosis inducing factor, Akt, phospho-Akt, Bad, and phospho-Bad. All of these data supported our initial hypothesis.

scholarly journals Perforin and Granzyme B Expressed by Murine Myeloid-Derived Suppressor Cells: A Study on Their Role in Outgrowth of Cancer Cells

Cancers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 808 ◽  
Author(s):  
Inès Dufait ◽  
Julian Pardo ◽  
David Escors ◽  
Yannick De Vlaeminck ◽  
Heng Jiang ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Granzyme B ◽  
Suppressor Cell ◽  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
Knock Out ◽  
Wide Range ◽  
B16f10 Melanoma Cells

A wide-range of myeloid-derived suppressor cell (MDSC)-mediated immune suppressive functions has previously been described. Nevertheless, potential novel mechanisms by which MDSCs aid tumor progression are, in all likelihood, still unrecognized. Next to its well-known expression in natural killer cells and cytotoxic T lymphocytes (CTLs), granzyme B (GzmB) expression has been found in different cell types. In an MDSC culture model, we demonstrated perforin and GzmB expression. Furthermore, similar observations were made in MDSCs isolated from tumor-bearing mice. Even in MDSCs from humans, GzmB expression was demonstrated. Of note, B16F10 melanoma cells co-cultured with perforin/GzmB knock out mice (KO) MDSCs displayed a remarkable decrease in invasive potential. B16F10 melanoma cells co-injected with KO MDSCs, displayed a significant slower growth curve compared to tumor cells co-injected with wild type (WT) MDSCs. In vivo absence of perforin/GzmB in MDSCs resulted in a higher number of CD8+ T-cells. Despite this change in favor of CD8+ T-cell infiltration, we observed low interferon-γ (IFN-γ) and high programmed death-ligand 1 (PD-L1) expression, suggesting that other immunosuppressive mechanisms render these CD8+ T-cells dysfunctional. Taken together, our results suggest that GzmB expression in MDSCs is another means to promote tumor growth and warrants further investigation to unravel the exact underlying mechanism.

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