scholarly journals Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes

2012 ◽  
Vol 29 (4) ◽  
pp. 593-600 ◽  
Author(s):  
FUQUAN LIN ◽  
WEN XU ◽  
CUIPING GUAN ◽  
MIAONI ZHOU ◽  
WEISONG HONG ◽  
...  
Keyword(s):  
Human Skin ◽  
Uvb Radiation ◽  
Skin Keratinocytes ◽  
Radiation Induced ◽  
Induced Apoptosis

scholarly journals Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

2019 ◽  
Vol 311 (3) ◽  
pp. 203-219 ◽  
Author(s):  
Agnieszka Gęgotek ◽  
Ewa Ambrożewicz ◽  
Anna Jastrząb ◽  
Iwona Jarocka-Karpowicz ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Human Skin ◽  
Uvb Radiation ◽  
Antiapoptotic Effect ◽  
Skin Keratinocytes

scholarly journals AMP-activated Protein Kinase Contributes to UV- and H2O2-induced Apoptosis in Human Skin Keratinocytes

2008 ◽  
Vol 283 (43) ◽  
pp. 28897-28908 ◽  
Author(s):  
Cong Cao ◽  
Shan Lu ◽  
Rebecca Kivlin ◽  
Brittany Wallin ◽  
Elizabeth Card ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Skin ◽  
Skin Keratinocytes ◽  
Induced Apoptosis ◽  
Amp Activated Protein Kinase

scholarly journals Coactivation of ATM/ERK/NF-κB in the low-dose radiation-induced radioadaptive response in human skin keratinocytes

2009 ◽  
Vol 46 (11) ◽  
pp. 1543-1550 ◽  
Author(s):  
Kazi Mokim Ahmed ◽  
Danupon Nantajit ◽  
Ming Fan ◽  
Jeffrey S. Murley ◽  
David J. Grdina ◽  
...  
Keyword(s):  
Human Skin ◽  
Low Dose ◽  
Low Dose Radiation ◽  
Skin Keratinocytes ◽  
Radiation Induced ◽  
Dose Radiation

Mechanisms of IFN-γ–induced apoptosis of human skin keratinocytes in patients with atopic dermatitis

2012 ◽  
Vol 129 (5) ◽  
pp. 1297-1306 ◽  
Author(s):  
Ana Rebane ◽  
Maya Zimmermann ◽  
Alar Aab ◽  
Hansjörg Baurecht ◽  
Andrea Koreck ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Skin Keratinocytes ◽  
Ifn Γ ◽  
Induced Apoptosis

scholarly journals Inhibitory Effect of Ursolic Acid on Ultraviolet B Radiation-Induced Oxidative Stress and Proinflammatory Response-Mediated Senescence in Human Skin Dermal Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Ramachandran Samivel ◽  
Rajendra Prasad Nagarajan ◽  
Umadevi Subramanian ◽  
Adnan Ali Khan ◽  
Ali Masmali ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Human Skin ◽  
Mitochondrial Membrane ◽  
Antioxidant Status ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Uvb Radiation ◽  
Proinflammatory Response ◽  
Radiation Induced ◽  
Apoptotic Induction

Ultraviolet radiation is an environmental carcinogenic agent that enhances inflammation and immunological reactions in the exposed human skin cells leading to oxidative photoaging of the epidermal and dermal segment. In the present study, we investigated the protective role of ursolic acid (UA) against ultraviolet B (UVB) radiation- induced photoaging an in vitro model of human skin dermal fibroblasts. UA-pretreated human skin dermal fibroblast (HDF) cells were exposed to UVB radiation to evaluated cell viability, reactive oxygen species (ROS), mitochondrial membrane potential, lipid peroxidation, antioxidant status, DNA damage, proinflammatory response, apoptotic induction, and matrix metalloproteinase (MMP) alteration. The UA pretreatment of HDFs mitigated the UVB irradiation-induced cytotoxicity, ROS generation, and mitochondrial membrane potential alteration and lipid peroxidation, depletion of antioxidant status, DNA damage, and apoptotic induction. UA pretreatment of HDFs also attenuated the UVB-induced expression of inflammatory (TNF-α and NF-κB) and apoptotic (p53, Bax, and caspase-3) and MMPs (MMP-2 and MMP-9) and enhanced the Bcl-2 protein levels in 20 μM UA treatment, when compared to concentrations. Hence, these results revealed that UA has the potential to mitigate UVB-induced extracellular damage by interfering with the ROS-mediated apoptotic induction and photoaging senescence and thus is a potential therapeutic agent to protect the skin against UVB-irradiation induced photooxidative damage.

scholarly journals Differential expression of selenoproteins by human skin cells and protection by selenium from UVB-radiation-induced cell death

1998 ◽  
Vol 332 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Teresa S. RAFFERTY ◽  
Roderick C. McKENZIE ◽  
John A. A. HUNTER ◽  
A. Forbes HOWIE ◽  
John R. ARTHUR ◽  
...  
Keyword(s):  
Cell Death ◽  
Human Skin ◽  
Sodium Selenite ◽  
Human Fibroblasts ◽  
Human Keratinocytes ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Skin Cells ◽  
Radiation Induced ◽  
Human Skin Cells

The generation of reactive oxygen species has been implicated as part of the mechanism responsible for UVB-radiation-induced skin damage. In mice, evidence suggests that increased dietary selenium intake may protect skin from many of the harmful effects of UVB radiation. We sought to determine the selenoprotein profile of cultured human skin cells and whether selenium supplementation could protect keratinocytes and melanocytes from the lethal effects of UVB radiation. Labelling experiments using [75Se]selenite showed qualitative and quantitative differences in selenoprotein expression by human fibroblasts, keratinocytes and melanocytes. This was most noticeable for thioredoxin reductase (60 kDa) and phospholipid glutathione peroxidase (21 kDa); these proteins were identified by Western blotting. Despite these differences, we found that a 24 h preincubation with sodium selenite or selenomethionine protected both cultured human keratinocytes and melanocytes from UVB-induced cell death. With primary keratinocytes, the greatest reduction in cell death was found with 10 nM sodium selenite (79% cell death reduced to 21.7%; P< 0.01) and with 50 nM selenomethionine (79% cell death reduced to 13.2%; P< 0.01). Protection could be obtained with concentrations as low as 1 nM with sodium selenite and 10 nM with selenomethionine. When selenium was added after UVB radiation, little protection could be achieved, with cell death only being reduced from 88.5% to about 50% with both compounds. In all of the experiments sodium selenite was more potent than selenomethionine at providing protection from UVB radiation.

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