Mechanisms of IFN-γ–induced apoptosis of human skin keratinocytes in patients with atopic dermatitis

2012 ◽  
Vol 129 (5) ◽  
pp. 1297-1306 ◽  
Author(s):  
Ana Rebane ◽  
Maya Zimmermann ◽  
Alar Aab ◽  
Hansjörg Baurecht ◽  
Andrea Koreck ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Skin Keratinocytes ◽  
Ifn Γ ◽  
Induced Apoptosis

scholarly journals Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes

2012 ◽  
Vol 29 (4) ◽  
pp. 593-600 ◽  
Author(s):  
FUQUAN LIN ◽  
WEN XU ◽  
CUIPING GUAN ◽  
MIAONI ZHOU ◽  
WEISONG HONG ◽  
...  
Keyword(s):  
Human Skin ◽  
Uvb Radiation ◽  
Skin Keratinocytes ◽  
Radiation Induced ◽  
Induced Apoptosis

scholarly journals AMP-activated Protein Kinase Contributes to UV- and H2O2-induced Apoptosis in Human Skin Keratinocytes

2008 ◽  
Vol 283 (43) ◽  
pp. 28897-28908 ◽  
Author(s):  
Cong Cao ◽  
Shan Lu ◽  
Rebecca Kivlin ◽  
Brittany Wallin ◽  
Elizabeth Card ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Skin ◽  
Skin Keratinocytes ◽  
Induced Apoptosis ◽  
Amp Activated Protein Kinase

scholarly journals New Potential of Roxatidine Acetate Hydrochloride on Atopic Dermatitis Mouse Model, Human Keratinocytes, and Human Skin Equivalent Model

2021 ◽  
Vol 12 ◽  
Author(s):  
Yun-Mi Kang ◽  
Minho Lee ◽  
Hyo-Jin An
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Human Skin ◽  
Equivalent Model ◽  
Hacat Keratinocytes ◽  
Roxatidine Acetate ◽  
Tnf Α ◽  
Ifn Γ ◽  
Human Skin Equivalent Model ◽  
Human Skin Equivalent

Atopic dermatitis (AD) is a complex inflammatory skin disorder, characterized by a complicated pathophysiology and a wide range of clinical phenotypes. Roxatidine acetate chloride (RXA) is a precursor of Roxatidine and a histamine H2 receptor antagonist, used for the treatment of gastric ulcers. In this study, we aimed to examine whether RXA had anti-AD effects and determine the underlying molecular mechanism of RXA. The anti-AD effects were examined in Dermatophagoides farinae body (Dfb)-induced AD mouse model, tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated HaCaT keratinocytes, and human skin equivalent model using ELISA, histological analysis, immunohistochemistry, Western blot, and immunofluorescence. Results showed that RXA treatment significantly alleviated Dfb-induced AD skin symptoms and clinical severity in mice by decreasing the levels of immunoglobulin E, histamine, and inflammatory cytokines. RXA effectively inhibited the expression of adhesive molecules and recovered the filaggrin expression in Dfb-induced AD skin lesions and TNF-α/IFN-γ-stimulated HaCaT keratinocytes. Additionally, RXA significantly upregulated the expression of aryl hydrocarbon receptor and sirtuin1. The anti-AD effects of RXA were associated with suppressed nuclear factor kappa cascade. Overall, our results suggest that RXA may be a potential anti-AD candidate owing to its inhibitory effect against skin inflammation and protection of the skin barrier function in AD.

scholarly journals AMP-activated protein kinase contributes to UV- and H2O2-induced apoptosis in human skin keratinocytes.

2010 ◽  
Vol 285 (19) ◽  
pp. 14842.2-14842 ◽  
Author(s):  
Cong Cao ◽  
Shan Lu ◽  
Rebecca Kivlin ◽  
Brittany Wallin ◽  
Elizabeth Card ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Skin ◽  
Skin Keratinocytes ◽  
Induced Apoptosis ◽  
Amp Activated Protein Kinase

Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes

2008 ◽  
Vol 311 (1-2) ◽  
pp. 111-119 ◽  
Author(s):  
Rupesh Dash ◽  
Mahitosh Mandal ◽  
Sudip K. Ghosh ◽  
S. C. Kundu
Keyword(s):  
Human Skin ◽  
Silk Sericin ◽  
Skin Keratinocytes ◽  
Tasar Silkworm ◽  
Induced Apoptosis

scholarly journals Impressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1

2021 ◽  
Vol 22 (5) ◽  
pp. 2334
Author(s):  
Jae Ho Choi ◽  
Gi Ho Lee ◽  
Sun Woo Jin ◽  
Ji Yeon Kim ◽  
Yong Pil Hwang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesions ◽  
Histopathological Analysis ◽  
Mrna Levels ◽  
Chemokine Production ◽  
Dermal Thickness ◽  
Skin Symptoms ◽  
Tnf Α ◽  
Ifn Γ ◽  
In Cells

Impressic acid (IPA), a lupane-type triterpenoid from Acanthopanax koreanum, has many pharmacological activities, including the attenuation of vascular endothelium dysfunction, cartilage destruction, and inflammatory diseases, but its influence on atopic dermatitis (AD)-like skin lesions is unknown. Therefore, we investigated the suppressive effect of IPA on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin symptoms in mice and the underlying mechanisms in cells. IPA attenuated the DNCB-induced increase in the serum concentrations of IgE and thymic stromal lymphopoietin (TSLP), and in the mRNA levels of thymus and activation regulated chemokine(TARC), macrophage derived chemokine (MDC), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) in mice. Histopathological analysis showed that IPA reduced the epidermal/dermal thickness and inflammatory and mast cell infiltration of ear tissue. In addition, IPA attenuated the phosphorylation of NF-κB and IκBα, and the degradation of IκBα in ear lesions. Furthermore, IPA treatment suppressed TNF-α/IFN-γ-induced TARC expression by inhibiting the NF-κB activation in cells. Phosphorylation of extracellular signalregulated protein kinase (ERK1/2) and the signal transducer and activator of transcription 1 (STAT1), the upstream signaling proteins, was reduced by IPA treatment in HaCaT cells. In conclusion, IPA ameliorated AD-like skin symptoms by regulating cytokine and chemokine production and so has therapeutic potential for AD-like skin lesions.

scholarly journals Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4409
Author(s):  
Jinjoo Kang ◽  
Soyoung Lee ◽  
Namkyung Kim ◽  
Hima Dhakal ◽  
Taeg-Kyu Kwon ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Skin Diseases ◽  
Nuclear Translocation ◽  
Skin Lesions ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Dermatophagoides Farinae ◽  
Tnf Α ◽  
Ifn Γ

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

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