scholarly journals UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study

2007 ◽  
Vol 168 (6) ◽  
pp. 698-705 ◽  
Author(s):  
Deleana Pozzi ◽  
Paola Grimaldi ◽  
Silvia Gaudenzi ◽  
Lucia Di Giambattista ◽  
Ida Silvestri ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Jurkat Cells ◽  
Uvb Radiation ◽  
Flow Cytometry Study ◽  
Radiation Induced ◽  
Induced Apoptosis

scholarly journals Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

2004 ◽  
Vol 18 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Silvia Gaudenzi ◽  
Deleana Pozzi ◽  
Paolo Toro ◽  
Ida Silvestri ◽  
Stefania Morrone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Ph Value ◽  
Fourier Transform Infrared ◽  
Specific Marker ◽  
Chemical Agent

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm−1and 2800 cm−1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‒destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologiesin vivo.

Multidisciplinary Investigations of a Double Sided Wooden Icon from Nicula Monastery, Romania

2019 ◽  
Vol 70 (8) ◽  
pp. 2747-2752
Author(s):  
Constantin Marutoiu ◽  
Ioan Bratu ◽  
Mircea Gelu Buta ◽  
Olivia Florena Nemes ◽  
Sergiu Petru Timbus(Monk Siluan) ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Spectroscopic Methods ◽  
X Ray ◽  
Painting Materials ◽  
The Status

A two-sided wooden icon from a monastery in Transylvania was submitted for multidisciplinary investigations involving X-Ray Fluorescence, Radiographic Photographyand Fourier Transform Infrared Spectroscopy. The most important part of the icon is St. Nicholas wooden icon, painted over forty years ago. The spectroscopic methods used revealed the painting materials composition, the status of the wooden stage, and the presence of resins as varnish (Fourier Transform Infrared Spectroscopy). On one side, the St Nicholasicon was painted over an old icon, St. Arch. Michael, which was evidenced by X-Ray Photography. The obtained data can serve for the preservation and the restoration of these wooden icons.

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