Multiplex Real-Time PCR Kit for Qualitative Detection of Genetically Modified Roundup Ready Soybean

2011 ◽  
Vol 54 (1) ◽  
Author(s):  
Jae-Hwan Kim
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Roundup Ready ◽  
Roundup Ready Soybean ◽  
Qualitative Detection

scholarly journals Quantitative Detection of Transgenic Roundup Ready Soybean Seeds Using Real-time PCR Method

2015 ◽  
Vol 1 (2) ◽  
pp. 71-78
Author(s):  
Elham Ghazizadeh ◽  
Amir Mousavi ◽  
Faranak Hadi ◽  
◽  
◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Detection ◽  
Soybean Seeds ◽  
Roundup Ready ◽  
Roundup Ready Soybean ◽  
Pcr Method

scholarly journals Detection and copy number estimation of the transgenic nucleotide sequences in an unknown GM event of Oryza sativa

2016 ◽  
Vol 69 (4) ◽  
Author(s):  
Ali M. Sajjad ◽  
Tanzeela Bashir ◽  
Shaina Saeed ◽  
Emmad Ahmad
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Genetically Modified ◽  
Marker Gene ◽  
Experimental Conditions ◽  
Rice Varieties ◽  
Genetically Modified Rice ◽  
Qualitative Detection

The present study was designed to establish a qualitative detection method based on conventional and real time PCR assay to screen the commonly grown rice varieties for the presence of the <em>cry1Ac</em> gene. The detection of genetically modified rice in the screening process would necessitate accurate assay development and precise qualitative PCR tests complying with established procedures for the detection and characterization of transgenes in food grains. Such assay would not only enable the monitoring of transgene flow in local agricultural environment but also the characterization of different plant species produced with this transgene and its regulatory components. Thus, a reliable and quick screening assay was established for the qualitative detection of the transgene along with the promoter and selectable marker gene in genetically modified rice. By conventional PCR, a fragment of 215 bp was amplified with gene specific primers of <em>cry1Ac</em>. Primers for other transgenes such as <em>gna</em> and <em>bar</em> were also employed; however, no amplification was detected. The presence of the <em>p35s</em>, <em>sps</em>, and <em>nptII</em> genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR products was checked through melt peak curve analysis. Sharp and precise melting temperatures indicated the presence of a single kind of PCR product in correspondence to each of the primers used. Moreover, the copy number of <em>cry1Ac</em> was estimated by ∆∆<em>C</em><span><sub>T</sub></span> method. It is proposed that the primer sets and experimental conditions used in this study will be sufficient to meet the requirements for molecular detection and characterization of the <em>cry1Ac</em> transgene and affiliated sequences in sorting out conventional rice varieties from the ones which are genetically modified. It will also help to monitor the ecological flow of these transgenes and other biosafety factors.

Real-time PCR multiplex method for the quantification of Roundup Ready soybean in raw material and processed food

2005 ◽  
Vol 222 (1-2) ◽  
pp. 209-216 ◽  
Author(s):  
Nicoletta Foti ◽  
Roberta Onori ◽  
Erica Donnarumma ◽  
Barbara De Santis ◽  
Marina Miraglia
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Raw Material ◽  
Processed Food ◽  
Roundup Ready ◽  
Roundup Ready Soybean ◽  
Pcr Multiplex

scholarly journals Detection of Unlabeled Genetically Modified Soybean in the Omani Market

2016 ◽  
Vol 21 (1) ◽  
pp. 1
Author(s):  
Nabila Al-Sadqi ◽  
Aliya S. Alansari
Keyword(s):  
Real Time ◽  
Quantitative Methods ◽  
Genetically Modified ◽  
Food Products ◽  
Detection Methods ◽  
Soybean Plant ◽  
Roundup Ready ◽  
Specific Pcr ◽  
Roundup Ready Soybean ◽  
Gm Soybean

This study aimed to screen for products containing Genetically Modified (GM) food in the Omani market using detection methods for the presence of Roundup Ready Soybean, Bt176 and MON810 maize in food products and to quantify it in positive samples using real time Polymerase Chain Reaction (PCR). A total of 100 food samples were collected randomly from markets in Oman. Out of 59 samples, 8  (13.5%) were successfully amplified with the maize plant specific PCR. GM screening showed negative for all samples, which indicated low or no GM maize in the samples tested. Out of 57 soy containing samples, 40 (70%) were successfully amplified by the soybean plant specific PCR. Six samples out of the 40 (15%) were found positive for GM using P35S-cf3/P35S-cr4 and HA-nos118-f/HA-nos118-r, primer pairs and using GMO5/GMO9 and GMO7/GMO8 primer pairs for specific detection of Roundup Ready Soybean. Real time PCR (TaqMan™ system) was carried out for the positive Roundup Ready Soybean samples and results showed that 2 out of the positive GM soy samples contained more than 5%; a Soy Formula for Infants (imported) sample contained 21% GM soybean and raw soybean seeds (imported in bulk amounts and packed in Oman)  contained 88% GM soybean. The results demonstrate for the first time the presence of GM-soy in food products in the Omani market, reinforcing the need for the use of qualitative and quantitative methods for GM detection in food products.  

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