Quantitative detection method for Roundup Ready soybean in food using duplex real-time PCR MGB chemistry

2010 ◽  
Vol 90 (9) ◽  
pp. 1437-1444 ◽  
Author(s):  
Maria Cristina Samson ◽  
Mariolina Gullì ◽  
Nelson Marmiroli
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Method ◽  
Quantitative Detection ◽  
Roundup Ready ◽  
Roundup Ready Soybean

scholarly journals Quantitative Detection of Transgenic Roundup Ready Soybean Seeds Using Real-time PCR Method

2015 ◽  
Vol 1 (2) ◽  
pp. 71-78
Author(s):  
Elham Ghazizadeh ◽  
Amir Mousavi ◽  
Faranak Hadi ◽  
◽  
◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Detection ◽  
Soybean Seeds ◽  
Roundup Ready ◽  
Roundup Ready Soybean ◽  
Pcr Method

Quantitative detection method of Enterocytozoon hepatopenaei using TaqMan probe real-time PCR

2018 ◽  
Vol 151 ◽  
pp. 191-196 ◽  
Author(s):  
Ya-Mei Liu ◽  
Liang Qiu ◽  
An-Zhi Sheng ◽  
Xiao-Yuan Wan ◽  
Dong-Yuan Cheng ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Method ◽  
Taqman Probe ◽  
Quantitative Detection ◽  
Enterocytozoon Hepatopenaei

Real-time PCR multiplex method for the quantification of Roundup Ready soybean in raw material and processed food

2005 ◽  
Vol 222 (1-2) ◽  
pp. 209-216 ◽  
Author(s):  
Nicoletta Foti ◽  
Roberta Onori ◽  
Erica Donnarumma ◽  
Barbara De Santis ◽  
Marina Miraglia
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Raw Material ◽  
Processed Food ◽  
Roundup Ready ◽  
Roundup Ready Soybean ◽  
Pcr Multiplex

scholarly journals Quantitative Detection of Clostridium perfringens in the Broiler Fowl Gastrointestinal Tract by Real-Time PCR

2005 ◽  
Vol 71 (7) ◽  
pp. 3911-3916 ◽  
Author(s):  
Mark G. Wise ◽  
Gregory R. Siragusa
Keyword(s):  
Gastrointestinal Tract ◽  
Real Time ◽  
Clostridium Perfringens ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Quantitative Detection ◽  
Necrotic Enteritis ◽  
Analytical Sensitivity ◽  
Quantitative Real Time Pcr ◽  
Pcr Inhibitor

ABSTRACT Strains of Clostridium perfringens are a frequent cause of food-borne disease and gas gangrene and are also associated with necrotic enteritis in chickens. To detect and quantify the levels of C. perfringens in the chicken gastrointestinal tract, a quantitative real-time PCR assay utilizing a fluorogenic, hydrolysis-type probe was developed and utilized to assay material retrieved from the broiler chicken cecum and ileum. Primers and probe were selected following an alignment of 16S rDNA sequences from members of cluster I of the genus Clostridium, and proved to be specific for C. perfringens. The assay could detect approximately 50 fg of C. perfringens genomic DNA and approximately 20 cells in pure culture. Measurements of the analytical sensitivity determined with spiked intestinal contents indicated that the consistent limit of detection with ileal samples was approximately 102 CFU/g of ileal material, but only about 104 CFU/g of cecal samples. The decreased sensitivity with the cecal samples was due to the presence of an unidentified chemical PCR inhibitor(s) in the cecal DNA purifications. The assay was utilized to rapidly detect and quantify C. perfringens levels in the gut tract of broiler chickens reared without supplementary growth-promoting antibiotics that manifested symptoms of necrotic enteritis. The results illustrated that quantitative real-time PCR correlates well with quantification via standard plate counts in samples taken from the ileal region of the gastrointestinal tract.

Quantitative Detection of Species-Specific DNA in Feedstuffs and Fish Meals

2005 ◽  
Vol 68 (6) ◽  
pp. 1217-1221 ◽  
Author(s):  
PAVEL KRCMAR ◽  
EVA RENCOVA
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Single Species ◽  
Absolute Quantification ◽  
Quantitative Detection ◽  
Vertebrate Species ◽  
Target Species ◽  
Species Specific

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species–derived material in concentrate feed mixtures and fish meals.

scholarly journals Application of real-time PCR for quantitative detection ofCampylobacter jejuniin poultry, milk and environmental water

2003 ◽  
Vol 38 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Chengbo Yang ◽  
Yuan Jiang ◽  
Kehe Huang ◽  
Changqing Zhu ◽  
Yulong Yin
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Environmental Water ◽  
Quantitative Detection

Quantitative detection of Escherichia coli from urine of patients with bacteriuria by real-time PCR

2004 ◽  
Vol 8 (3) ◽  
pp. 179-184 ◽  
Author(s):  
Nobuyuki Hinata ◽  
Toshiro Shirakawa ◽  
Hiroshi Okada ◽  
Katsumi Shigemura ◽  
Sadao Kamidono ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Detection
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