Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods

1999 ◽  
Vol 47 (12) ◽  
pp. 5261-5266 ◽  
Author(s):  
Marc Vaïtilingom ◽  
Hans Pijnenburg ◽  
François Gendre ◽  
Pierre Brignon
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Pcr Detection ◽  
Roundup Ready ◽  
Real Time Quantitative Pcr ◽  
Roundup Ready Soybean

Analysis of Caecal Microbiota in Rats Fed with Genetically Modified Rice by Real-Time Quantitative PCR

2011 ◽  
Vol 76 (1) ◽  
pp. M88-M93 ◽  
Author(s):  
Wentao Xu ◽  
Liting Li ◽  
Jiao Lu ◽  
YunBo Luo ◽  
Ying Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Real Time Quantitative Pcr ◽  
Caecal Microbiota

scholarly journals Effects of DNA Extraction and Purification Methods on Real-Time Quantitative PCR Analysis of Roundup Ready Soybean

2009 ◽  
Vol 92 (4) ◽  
pp. 1136-1144 ◽  
Author(s):  
Tigst Demeke ◽  
Indira Ratnayaka ◽  
Anh Phan
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Roundup Ready ◽  
Dna Recovery ◽  
Real Time Quantitative Pcr ◽  
Extraction And Purification ◽  
Purification Methods ◽  
Accuracy And Repeatability

Abstract The quality of DNA affects the accuracy and repeatability of quantitative PCR results. Different DNA extraction and purification methods were compared for quantification of Roundup Ready (RR) soybean (event 40-3-2) by real-time PCR. DNA was extracted using cetylmethylammonium bromide (CTAB), DNeasy Plant Mini Kit, and Wizard Magnetic DNA purification system for food. CTAB-extracted DNA was also purified using the Zymo (DNA Clean & Concentrator 25 kit), Qtip 100 (Qiagen Genomic-Tip 100/G), and QIAEX II Gel Extraction Kit. The CTAB extraction method provided the largest amount of DNA, and the Zymo purification kit resulted in the highest percentage of DNA recovery. The Abs260/280 and Abs260/230 ratios were less than the expected values for some of the DNA extraction and purification methods used, indicating the presence of substances that could inhibit PCR reactions. Real-time quantitative PCR results were affected by the DNA extraction and purification methods used. Further purification or dilution of the CTAB DNA was required for successful quantification of RR soybean. Less variability of quantitative PCR results was observed among experiments and replications for DNA extracted and/or purified by CTAB, CTAB+Zymo, CTAB+Qtip 100, and DNeasy methods. Correct and repeatable results for real-time PCR quantification of RR soybean were achieved using CTAB DNA purified with Zymo and Qtip 100 methods.

A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods

2007 ◽  
Vol 71 (12) ◽  
pp. 3131-3135 ◽  
Author(s):  
Soichi TANABE ◽  
Makiko HASE ◽  
Takeo YANO ◽  
Masahiko SATO ◽  
Tatsuya FUJIMURA ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Detection Method ◽  
Pcr Detection ◽  
Real Time Quantitative Pcr
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