scholarly journals Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros sp. (strain TG07-C28)

2012 ◽  
Vol 7 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Kensuke Toyoda ◽  
Kei Kimura ◽  
Naotsugu Hata ◽  
Natsuko Nakayama ◽  
Keizo Nagasaki ◽  
...  
Keyword(s):  
Dna Virus ◽  
Single Stranded Dna ◽  
Isolation And Characterization ◽  
Planktonic Diatom

Characterization of a complete genome of a circular single-stranded DNA virus from porcine stools in Korea

Virus Genes ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 81-88 ◽  
Author(s):  
A Reum Kim ◽  
Hee Chun Chung ◽  
Hye Kwon Kim ◽  
Eun Ok Kim ◽  
Van Giap Nguyen ◽  
...  
Keyword(s):  
Complete Genome ◽  
Dna Virus ◽  
Single Stranded Dna

ISOLATION AND CHARACTERIZATION OF A DNA VIRUS INFECTING FELDMANNIA SIMPLEX (PHAEOPHYCEAE)1

1994 ◽  
Vol 30 (4) ◽  
pp. 653-658 ◽  
Author(s):  
Anne-K. Friess-Klebl ◽  
Rolf Knippers ◽  
Dieter G. Muller
Keyword(s):  
Dna Virus ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Single-Stranded DNA Virus Infecting Chaetoceros lorenzianus Grunow

2011 ◽  
Vol 77 (15) ◽  
pp. 5285-5293 ◽  
Author(s):  
Yuji Tomaru ◽  
Yoshitake Takao ◽  
Hidekazu Suzuki ◽  
Tamotsu Nagumo ◽  
Kanae Koike ◽  
...  
Keyword(s):  
Burst Size ◽  
Open Reading Frames ◽  
Dna Virus ◽  
Single Stranded Dna ◽  
Content Type ◽  
Virus Particles ◽  
Isolation And Characterization ◽  
Double Stranded Dna ◽  
Circular Ssdna ◽  
Related Proteins

ABSTRACTDiatoms are one of the most significant primary producers in the ocean, and the importance of viruses as a potential source of mortality for diatoms has recently been recognized. Thus far, eight different diatom viruses infecting the generaRhizosoleniaandChaetoceroshave been isolated and characterized to different extents. We report the isolation of a novel diatom virus (ClorDNAV), which causes the lysis of the bloom-forming speciesChaetoceros lorenzianus, and show its physiological, morphological, and genomic characteristics. The free virion was estimated to be ∼34 nm in diameter. The arrangement of virus particles appearing in cross-section was basically a random aggregation in the nucleus. Occasionally, distinctive formations such as a ring-like array composed of 9 or 10 spherical virions or a centipede-like array composed of rod-shaped particles were also observed. The latent period and the burst size were estimated to be <48 h and 2.2 × 104infectious units per host cell, respectively. ClorDNAV harbors a covalently closed circular single-stranded DNA (ssDNA) genome (5,813 nucleotides [nt]) that includes a partially double-stranded DNA region (979 nt). At least three major open reading frames were identified; one showed a high similarity to putative replicase-related proteins of the other ssDNA diatom viruses,Chaetoceros salsugineumDNA virus (previously reported as CsNIV) andChaetoceros tenuissimusDNA virus. ClorDNAV is the third member of the closed circular ssDNA diatom virus group, the genusBacilladnavirus.

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