scholarly journals Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties

2005 ◽  
Vol 33 (1) ◽  
pp. 135-142 ◽  
Author(s):  
T. Blondal
Keyword(s):  
Single Stranded Dna ◽  
Rna Ligase ◽  
Isolation And Characterization ◽  
Dna Ligation

scholarly journals Isolation and characterization of an RNA ligase from HeLa cells.

1985 ◽  
Vol 82 (3) ◽  
pp. 684-688 ◽  
Author(s):  
K. K. Perkins ◽  
H. Furneaux ◽  
J. Hurwitz
Keyword(s):  
Hela Cells ◽  
Rna Ligase ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of RNA ligase from wheat germ.

1983 ◽  
Vol 80 (13) ◽  
pp. 3933-3937 ◽  
Author(s):  
H. Furneaux ◽  
L. Pick ◽  
J. Hurwitz
Keyword(s):  
Wheat Germ ◽  
Rna Ligase ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros sp. (strain TG07-C28)

2012 ◽  
Vol 7 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Kensuke Toyoda ◽  
Kei Kimura ◽  
Naotsugu Hata ◽  
Natsuko Nakayama ◽  
Keizo Nagasaki ◽  
...  
Keyword(s):  
Dna Virus ◽  
Single Stranded Dna ◽  
Isolation And Characterization ◽  
Planktonic Diatom

scholarly journals Isolation and characterization of a processive DNA helicase from the fission yeast Schizosaccharomyces pombe that translocates in a 5′-to-3′ direction

1998 ◽  
Vol 334 (2) ◽  
pp. 377-386 ◽  
Author(s):  
Changwoo LEE ◽  
Yeon-Soo SEO
Keyword(s):  
Atpase Activity ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Atp Hydrolysis ◽  
Sedimentation Coefficient ◽  
Dna Helicase ◽  
Single Stranded Dna ◽  
Dna Helicase Ii ◽  
Isolation And Characterization

We report here the isolation and characterization of a novel DNA helicase from extracts of the fission yeast Schizosaccharomyces pombe. The enzyme, called DNA helicase II, also contains an intrinsic DNA-dependent ATPase activity. Both the helicase and ATPase activities co-purified with a 63 kDa polypeptide on an SDS/polyacrylamide gel. The protein has a sedimentation coefficient of 4.8 S and a Stokes radius of 36 Å (3.6 nm); from these data the native molecular mass was calculated to be 65 kDa. The enzyme translocates in a 5´-to-3´ direction with respect to the substrate strand to which it is bound. Unwinding reactions carried out in the presence of increasing enzyme showed a sigmoidal curve, suggesting either co-operative interactions between monomers or multimerization of DNA helicase II in the presence of single-stranded DNA and/or ATP. This enzyme favoured adenosine nucleotides (ATP and dATP) as its energy source, but utilized to limited extents GTP, CTP, dGTP and dCTP. Non-hydrolysable ATP analogues did not support helicase activity. Kinetic analyses showed that the unwinding reaction was rapid, being complete after 50–100 s of incubation. Addition of unlabelled substrates to the helicase reaction after preincubation of the enzyme with substrate did not significantly diminish unwinding. The ATPase activity of DNA helicase II increased proportionally with increasing lengths of single-stranded DNA cofactor. In the presence of circular DNA, ATP hydrolysis continued to increase up to the longest time tested (3 h), whereas it ceased to increase after 5–10 min in the presence of shorter oligonucleotides. The initial rate of ATP hydrolysis during the first 5 min of incubation time was not affected by DNA species used. These data indicate that the enzyme does not dissociate from the single-stranded DNA once it is bound and is therefore highly processive.

Sign in / Sign up

Export Citation Format

Share Document