scholarly journals Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program

10.3791/62260 ◽  
2021 ◽  
Author(s):  
Bianca J. Moreno ◽  
Robert L. Aldridge ◽  
Seth C. Britch ◽  
Barbara E. Bayer ◽  
Jedidiah Kline ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sterile Insect Technique ◽  
Sterile Insect Technique Program

scholarly journals Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0212520 ◽  
Author(s):  
J. Guillermo Bond ◽  
Adriana R. Osorio ◽  
Nancy Avila ◽  
Yeudiel Gómez-Simuta ◽  
Carlos F. Marina ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sterile Insect Technique ◽  
Irradiation Dose ◽  
Sterile Insect Technique Program

Investigation of Developmental Stage/Age, Gamma Irradiation Dose, and Temperature in Sterilization of Male Aedes aegypti (Diptera: Culicidae) in a Sterile Insect Technique Program

2021 ◽  
Author(s):  
Beni Ernawan ◽  
Tjandra Anggraeni ◽  
Sri Yusmalinar ◽  
Intan Ahmad
Keyword(s):  
Aedes Aegypti ◽  
Gamma Irradiation ◽  
Sterile Insect Technique ◽  
Irradiation Dose ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Standard Protocol ◽  
Mating Competitiveness ◽  
Sterile Insect Technique Program ◽  
High Level

Abstract The sterilization process using gamma irradiation is a crucial component in a program using sterile insect technique (SIT) to control Aedes aegypti. Unfortunately, there is no efficient standard protocol for sterilizing mosquitoes that can produce a high level of sterility while maintaining mating ability and longevity. Therefore, we conducted a study of the critical factors necessary to develop such a standard protocol. In this study, male Ae. aegypti pupae, as well as adults aged 1 d and 3 d, were irradiated using a Gamma-cell 220 irradiator doses of 0, 20, 40, 60, 70, 80, and 100 Gray (Gy). In addition, male Ae. aegypti in the pupal and adult stage aged 1 d were irradiated at a dose of 70 Gy at various temperatures. Changes in emergence rates, longevity, sterility, and mating competitiveness were recorded for each combination of parameters. Results showed that an increase of irradiation dose leads to a rise of induced sterility at all developmental stages, while simultaneously reducing emergence rate, survival, and mating competitiveness. Higher temperatures resulted in increased levels of sterility, reduced longevity, and did not affect the ability to mate. This study found that an irradiation dose of 70 Gy at a temperature between 20.00 and 22.30°C administered in the pupal stage induced a high level of sterility (around 98%), while maintaining mating competitiveness and longevity.

scholarly journals Aedes aegypti lines for combined sterile insect technique and incompatible insect technique applications: the importance of host genomic background

2020 ◽  
Vol 168 (6-7) ◽  
pp. 560-572 ◽  
Author(s):  
Danilo O. Carvalho ◽  
Jorge A. Torres‐Monzon ◽  
Panagiota Koskinioti ◽  
N.D. Asha Dilrukshi Wijegunawardana ◽  
Xiao Liang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sterile Insect Technique ◽  
Incompatible Insect Technique

scholarly journals Suppressing mosquito populations with precision guided sterile males

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ming Li ◽  
Ting Yang ◽  
Michelle Bui ◽  
Stephanie Gamez ◽  
Tyler Wise ◽  
...  
Keyword(s):  
Control System ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Sterile Insect Technique ◽  
Life Stage ◽  
Molecular Genetic ◽  
Control Methods ◽  
Wild Populations ◽  
Principal Vector ◽  
Platform Technology

AbstractThe mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.

Optimal Control of Aedes Aegypti Mosquitoes by Sterile Insect Technique, Insecticide and Larvicide

2015 ◽  
Author(s):  
Roberto Carlos Antunes Thomé ◽  
Claudia Mazza Dias ◽  
Edilson Fernandes Arruda ◽  
Dayse Haime Pastore ◽  
Hyun Mo Yang
Keyword(s):  
Optimal Control ◽  
Aedes Aegypti ◽  
Sterile Insect Technique

ASSESSING THE SUITABILITY OF STERILE INSECT TECHNIQUE APPLIED TOAEDES AEGYPTI

2008 ◽  
Vol 16 (04) ◽  
pp. 565-577 ◽  
Author(s):  
CLAUDIA PIO FERREIRA ◽  
HYUN MO YANG ◽  
LOURDES ESTEVA
Keyword(s):  
Biological Control ◽  
Cellular Automata ◽  
Aedes Aegypti ◽  
Control Strategy ◽  
Sterile Insect Technique ◽  
Alternative Control ◽  
Heterogeneous Distribution ◽  
Insecticide Application ◽  
Mosquito Behavior

The efficacy of biological control of Aedes aegypti mosquitoes using Sterile Insect Technique (SIT) is analyzed. This approach has shown to be very efficient on agricultural plagues and has become an alternative control strategy to the usual technique of insecticide application, which promotes resistance against chemical controls and is harmful to other species that live in the same mosquito habitat. By using a discrete cellular automata approach we have shown that in the case of Aedes aegypti, the spatially heterogeneous distribution of oviposition containers and the mosquito behavior, especially with respect to mating, make the application of STI difficult or impracticable.

scholarly journals Dampak Iradiasi Sinar Gamma pada Produktivitas Aedes aegypti Jantan

2019 ◽  
Vol 13 (1) ◽  
pp. 1-6
Author(s):  
MIRNA WATI DEWI ◽  
Susi Soviana ◽  
Umi Cahyaningsih ◽  
Ali Rahayu
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Sterile Insect Technique ◽  
Second Generation ◽  
Gamma Ray ◽  
Vector Borne Disease ◽  
Egg Hatchability ◽  
Negative Effect ◽  
Gamma Ray Irradiation ◽  
Vector Borne

Abstract Dengue fever is a vector-borne disease with Aedes aegypti as the main vector. Vector controls currently depended on insecticide. Considering the negative effect of insecticide, Sterile Insect Technique (SIT) was developed. This study was conducted to evaluate the effect of gamma-ray irradiation on the productivity of male Ae. aegypti. Male pupae age less than 15 hours were irradiated with 60 Gy and 70 Gy gamma-ray. When the pupae became adult, the sterile males mated with the same age females Aedes aegypti. Observation on fecundity, hatchability, and age was carried out until the second generation. Gamma-ray irradiation with the dose of 60 Gy and 70 Gy showed different effects on fecundity, egg hatchability, the emergence of the adult, and age of Aedes aegypti compared to control. Abstrak Demam berdarah merupakan penyakit tular vektor  yang sampai saat ini masih menjadi masalah kesehatan secara global. Vektor utama yang berperan pada penyebaran penyakit DBD yaitu nyamuk Aedes aegytpi. Pengendalian vektor saat ini sangat bergantung pada penggunaan insektisida. Dampak negatif penggunaan insektisida menyebabkan pengembangan pengendalian vektor yang lain diantaranya yaitu Teknik Serangga Mandul (TSM). Penelitian ini dilakukan untuk menganalisis produktivitas Ae. aegypti jantan iradiasi hingga generasi kedua. Pupa jantan umur <15 jam diradiasi sinargamma dosis 60 Gy dan 70 Gy. Setelah menjadi nyamuk jantan dewasa segeradikawinkan dengan nyamuk betina tidak iradiasi dengan umur yang sama. Pengamatan dilakukan terutama terhadap fekunditas, daya tetas, kemunculan nyamuk, dan umurnyamuk hingga generasi kedua. Iradiasi sinar gamma dosis 60 Gy dan 70 Gymenghasilkan dampak yang berbeda terhadap fekunditas, daya tetas telur, kemunculan nyamuk dan umur nyamuk bila dibandingkan dengan kontrol.

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