scholarly journals Aedes aegypti lines for combined sterile insect technique and incompatible insect technique applications: the importance of host genomic background

2020 ◽  
Vol 168 (6-7) ◽  
pp. 560-572 ◽  
Author(s):  
Danilo O. Carvalho ◽  
Jorge A. Torres‐Monzon ◽  
Panagiota Koskinioti ◽  
N.D. Asha Dilrukshi Wijegunawardana ◽  
Xiao Liang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sterile Insect Technique ◽  
Incompatible Insect Technique

scholarly journals Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand

2019 ◽  
Vol 13 (10) ◽  
pp. e0007771 ◽  
Author(s):  
Pattamaporn Kittayapong ◽  
Suwannapa Ninphanomchai ◽  
Wanitch Limohpasmanee ◽  
Chitti Chansang ◽  
Uruyakorn Chansang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sterile Insect Technique ◽  
Proof Of Concept ◽  
Incompatible Insect Technique ◽  
Rural Settings

scholarly journals Use of mechanical and behavioural methods to eliminate female Aedes aegypti and Aedes albopictus for sterile insect technique and incompatible insect technique applications

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Nayana Gunathilaka ◽  
Tharaka Ranathunge ◽  
Lahiru Udayanga ◽  
Asha Wijegunawardena ◽  
Jeremie Roger Lionel Gilles ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Sterile Insect Technique ◽  
Incompatible Insect Technique

scholarly journals Suppressing mosquito populations with precision guided sterile males

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ming Li ◽  
Ting Yang ◽  
Michelle Bui ◽  
Stephanie Gamez ◽  
Tyler Wise ◽  
...  
Keyword(s):  
Control System ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Sterile Insect Technique ◽  
Life Stage ◽  
Molecular Genetic ◽  
Control Methods ◽  
Wild Populations ◽  
Principal Vector ◽  
Platform Technology

AbstractThe mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.

Optimal Control of Aedes Aegypti Mosquitoes by Sterile Insect Technique, Insecticide and Larvicide

2015 ◽  
Author(s):  
Roberto Carlos Antunes Thomé ◽  
Claudia Mazza Dias ◽  
Edilson Fernandes Arruda ◽  
Dayse Haime Pastore ◽  
Hyun Mo Yang
Keyword(s):  
Optimal Control ◽  
Aedes Aegypti ◽  
Sterile Insect Technique

ASSESSING THE SUITABILITY OF STERILE INSECT TECHNIQUE APPLIED TOAEDES AEGYPTI

2008 ◽  
Vol 16 (04) ◽  
pp. 565-577 ◽  
Author(s):  
CLAUDIA PIO FERREIRA ◽  
HYUN MO YANG ◽  
LOURDES ESTEVA
Keyword(s):  
Biological Control ◽  
Cellular Automata ◽  
Aedes Aegypti ◽  
Control Strategy ◽  
Sterile Insect Technique ◽  
Alternative Control ◽  
Heterogeneous Distribution ◽  
Insecticide Application ◽  
Mosquito Behavior

The efficacy of biological control of Aedes aegypti mosquitoes using Sterile Insect Technique (SIT) is analyzed. This approach has shown to be very efficient on agricultural plagues and has become an alternative control strategy to the usual technique of insecticide application, which promotes resistance against chemical controls and is harmful to other species that live in the same mosquito habitat. By using a discrete cellular automata approach we have shown that in the case of Aedes aegypti, the spatially heterogeneous distribution of oviposition containers and the mosquito behavior, especially with respect to mating, make the application of STI difficult or impracticable.

scholarly journals Wolbachia-mediated sterility suppresses Aedes aegypti populations in the urban tropics

2021 ◽  
Author(s):  
◽  
Lee Ching Ng
Keyword(s):  
Aedes Aegypti ◽  
Vertical Distribution ◽  
High Rise ◽  
Promising Tool ◽  
Incompatible Insect Technique ◽  
Tropical Cities ◽  
Dengue Control ◽  
Housing Estates

Incompatible insect technique (IIT) via releases of male Wolbachia-infected mosquitoes is a promising tool for dengue control. In a three-year trial in Singaporean high-rise housing estates, we demonstrated that Wolbachia-based IIT dramatically reduces both wildtype Aedes aegypti populations [reductions of 92.7% (95% CI: 84.7%-95.8%) and 98.3% (97.7%-99.8%)] and dengue incidence [reductions of 71% (43%-87%) to 88% (57%-99%)] in the targeted areas. The study highlights the need to ensure adequate vertical distribution of released males in high-rise buildings, address immigration of wildtype females from neighboring areas, and prevent and mitigate stable establishment of Wolbachia in field mosquito populations. Our results demonstrate the potential of Wolbachia-based IIT (supplemented with irradiation, in Singapore's context) for strengthening dengue control in tropical cities, where dengue burden is the greatest.

scholarly journals Dampak Iradiasi Sinar Gamma pada Produktivitas Aedes aegypti Jantan

2019 ◽  
Vol 13 (1) ◽  
pp. 1-6
Author(s):  
MIRNA WATI DEWI ◽  
Susi Soviana ◽  
Umi Cahyaningsih ◽  
Ali Rahayu
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Sterile Insect Technique ◽  
Second Generation ◽  
Gamma Ray ◽  
Vector Borne Disease ◽  
Egg Hatchability ◽  
Negative Effect ◽  
Gamma Ray Irradiation ◽  
Vector Borne

Abstract Dengue fever is a vector-borne disease with Aedes aegypti as the main vector. Vector controls currently depended on insecticide. Considering the negative effect of insecticide, Sterile Insect Technique (SIT) was developed. This study was conducted to evaluate the effect of gamma-ray irradiation on the productivity of male Ae. aegypti. Male pupae age less than 15 hours were irradiated with 60 Gy and 70 Gy gamma-ray. When the pupae became adult, the sterile males mated with the same age females Aedes aegypti. Observation on fecundity, hatchability, and age was carried out until the second generation. Gamma-ray irradiation with the dose of 60 Gy and 70 Gy showed different effects on fecundity, egg hatchability, the emergence of the adult, and age of Aedes aegypti compared to control. Abstrak Demam berdarah merupakan penyakit tular vektor  yang sampai saat ini masih menjadi masalah kesehatan secara global. Vektor utama yang berperan pada penyebaran penyakit DBD yaitu nyamuk Aedes aegytpi. Pengendalian vektor saat ini sangat bergantung pada penggunaan insektisida. Dampak negatif penggunaan insektisida menyebabkan pengembangan pengendalian vektor yang lain diantaranya yaitu Teknik Serangga Mandul (TSM). Penelitian ini dilakukan untuk menganalisis produktivitas Ae. aegypti jantan iradiasi hingga generasi kedua. Pupa jantan umur <15 jam diradiasi sinargamma dosis 60 Gy dan 70 Gy. Setelah menjadi nyamuk jantan dewasa segeradikawinkan dengan nyamuk betina tidak iradiasi dengan umur yang sama. Pengamatan dilakukan terutama terhadap fekunditas, daya tetas, kemunculan nyamuk, dan umurnyamuk hingga generasi kedua. Iradiasi sinar gamma dosis 60 Gy dan 70 Gymenghasilkan dampak yang berbeda terhadap fekunditas, daya tetas telur, kemunculan nyamuk dan umur nyamuk bila dibandingkan dengan kontrol.

scholarly journals Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia

2021 ◽  
Vol 118 (41) ◽  
pp. e2106828118
Author(s):  
Nigel W. Beebe ◽  
Dan Pagendam ◽  
Brendan J. Trewin ◽  
Andrew Boomer ◽  
Matt Bradford ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Local Strain ◽  
Mosquito Vector ◽  
Population Declines ◽  
Wild Type ◽  
Strong Suppression ◽  
Incompatible Insect Technique ◽  
Community Consent ◽  
Proven Method

Releasing sterile or incompatible male insects is a proven method of population management in agricultural systems with the potential to revolutionize mosquito control. Through a collaborative venture with the “Debug” Verily Life Sciences team, we assessed the incompatible insect technique (IIT) with the mosquito vector Aedes aegypti in northern Australia in a replicated treatment control field trial. Backcrossing a US strain of Ae. aegypti carrying Wolbachia wAlbB from Aedes albopictus with a local strain, we generated a wAlbB2-F4 strain incompatible with both the wild-type (no Wolbachia) and wMel-Wolbachia Ae. aegypti now extant in North Queensland. The wAlbB2-F4 strain was manually mass reared with males separated from females using Verily sex-sorting technologies to obtain no detectable female contamination in the field. With community consent, we delivered a total of three million IIT males into three isolated landscapes of over 200 houses each, releasing ∼50 males per house three times a week over 20 wk. Detecting initial overflooding ratios of between 5:1 and 10:1, strong population declines well beyond 80% were detected across all treatment landscapes when compared to controls. Monitoring through the following season to observe the ongoing effect saw one treatment landscape devoid of adult Ae. aegypti early in the season. A second landscape showed reduced adults, and the third recovered fully. These encouraging results in suppressing both wild-type and wMel-Ae. aegypti confirms the utility of bidirectional incompatibility in the field setting, show the IIT to be robust, and indicate that the removal of this arbovirus vector from human-occupied landscapes may be achievable.

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