Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

10.3791/55989 ◽  
2019 ◽  
Author(s):  
Zackary N. Scholl ◽  
Qing Li ◽  
Eric Josephs ◽  
Dimitra Apostolidou ◽  
Piotr E. Marszalek
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Atomic Force ◽  
Protein Molecules ◽  
Single Protein

Probing the mechanical stability of proteins using the atomic force microscope

2007 ◽  
Vol 35 (6) ◽  
pp. 1564-1568 ◽  
Author(s):  
D.J. Brockwell
Keyword(s):  
Mechanical Strength ◽  
Atomic Force Microscope ◽  
Mechanical Stability ◽  
Mechanical Deformation ◽  
Mechanical Resistance ◽  
Atomic Force ◽  
Wide Range ◽  
Protein Molecules ◽  
Single Protein

The mechanical strength of single protein molecules can be investigated by using the atomic force microscope. By applying this technique to a wide range of proteins, it appears that the type of secondary structure and its orientation relative to the extension points are important determinants of mechanical strength. Unlike chemical denaturants, force acts locally and the mechanical strength of a protein may thus appear to be mechanically weak or strong by simply varying the region of the landscape through which the protein is unfolded. Similarly, the effect of ligand binding on the mechanical resistance of a protein may also depend on the relative locations of the binding site and force application. Mechanical deformation may thus facilitate the degradation or remodelling of thermodynamically stable proteins and their complexes in vivo.

Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding

2011 ◽  
Vol 414 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Hans A. Heus ◽  
Elias M. Puchner ◽  
Aafke J. van Vugt-Jonker ◽  
Julia L. Zimmermann ◽  
Hermann E. Gaub
Keyword(s):  
Atomic Force Microscope ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Atomic Force

Midas: A Nanotechnological Exploration of Touch

Leonardo ◽  
2009 ◽  
Vol 42 (3) ◽  
pp. 186-192 ◽  
Author(s):  
Paul Thomas
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Atomic Force ◽  
Atomic Vibrations ◽  
Surface Vibrations

The Midas project investigates the trans-mediational space between skin and gold. Research for the project was conducted through the analysis of data recorded with an Atomic Force Microscope (AFM). The AFM, in its force spectroscopy mode, gathers data by picking up the surface vibrations as the cantilever touches the cell. The Midas project culminated in an installation that included data projection and audio work utilizing subsonic speakers to make the data from the atomic vibrations audible and palpable.

Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers

1999 ◽  
Vol 70 (11) ◽  
pp. 4300-4303 ◽  
Author(s):  
M. B. Viani ◽  
T. E. Schäffer ◽  
G. T. Paloczi ◽  
L. I. Pietrasanta ◽  
B. L. Smith ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Fast Imaging ◽  
Atomic Force

scholarly journals A Tiny Toolbox

1997 ◽  
Vol 5 (7) ◽  
pp. 3-7
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Atomic Force Microscope ◽  
Single Molecule ◽  
Recent Article ◽  
Single Molecules ◽  
Force Spectroscopy ◽  
Atomic Level ◽  
Single Molecule Force Spectroscopy ◽  
Binding Force ◽  
Atomic Force

A recent article by Matthias Rief, Filipp Oesterhelt. Berthold Heymann, and Hermann Gaub concluded with this sentence: "Single molecule force spectroscopy by AFM has proven to be a powerful addition to the nanoscopic piconewton toolbox," Everything about that conclusion is tiny. Clearly, the atomic force microscope (AFM) has given us a tool to examine structure at or near the atomic level. Earlier work from Gaub's laboratory, reviewed in this column, demonstrated that the AFM could directly measure the binding force between single molecules of biotin and avidin. This established that the AFM could be used as a tool to measure forces, not just observe structure. Their most recent experiments has added to this tiny toolbox.

scholarly journals Investigating pore-forming peptides using the atomic force microscope

2019 ◽  
Author(s):  
◽  
Anna Elizabeth Pittman
Keyword(s):  
Atomic Force Microscope ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Temporal Stability ◽  
Force Spectroscopy ◽  
Hydrodynamic Drag ◽  
Gold Coating ◽  
Cross Sectional ◽  
Lipid Interactions ◽  
Atomic Force

In my projects, I used the Atomic Force Microscope (AFM) to image the effects of pore-forming peptides and I used the Focused Ion Beam (FIB) to modify AFM cantilevers to increase the precision of force spectroscopy measurements. ... Another way to investigate peptide-lipid interactions is to perform force spectroscopy experiments using the AFM. In order to lower drift and increase force precision, I used a FIB to modify commercially available AFM cantilevers. By reducing the cross sectional area of the cantilever, the hydrodynamic drag was reduced, thus increasing the force precision. Removing most of the gold coating on the cantilever increased the temporal stability. These modified cantilevers have already been put to use in the lab to measure peptide-lipid interactions.

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