Force spectroscopy based on temperature controlled atomic force microscope head using piezoresistive cantilevers

2005 ◽  
Vol 76 (4) ◽  
pp. 043701 ◽  
Author(s):  
I. Muševič ◽  
K. Kočevar ◽  
U. Kržič ◽  
G. Carbone
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Atomic Force ◽  
Piezoresistive Cantilevers ◽  
Temperature Controlled

Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding

2011 ◽  
Vol 414 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Hans A. Heus ◽  
Elias M. Puchner ◽  
Aafke J. van Vugt-Jonker ◽  
Julia L. Zimmermann ◽  
Hermann E. Gaub
Keyword(s):  
Atomic Force Microscope ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Atomic Force

scholarly journals Atomic force microscope with an adjustable probe direction and piezoresistive cantilevers operated in tapping-mode / Im Tapping-Modus betriebenes Rasterkraftmikroskop mit einstellbarer Antastrichtung und piezoresistiven Cantilevern

2019 ◽  
Vol 86 (s1) ◽  
pp. 12-16
Author(s):  
Janik Schaude ◽  
Julius Albrecht ◽  
Ute Klöpzig ◽  
Andreas C. Gröschl ◽  
Tino Hausotte
Keyword(s):  
Atomic Force Microscope ◽  
Metrological Traceability ◽  
Step Height ◽  
Tapping Mode ◽  
Atomic Force ◽  
In Situ Calibration ◽  
Measuring Machine ◽  
Piezoresistive Cantilevers

AbstractThis article presents a new tilting atomic force microscope (AFM) with an adjustable probe direction and piezoresistive cantilever operated in tapping-mode. The AFM is based on two rotational axes, which enable the adjustment of the probe direction to cover a complete hemisphere. The whole setup is integrated into a nano measuring machine (NMM-1) and the metrological traceability of the piezoresistive cantilever is warranted by in situ calibration on the NMM-1. To demonstrate the capabilities of the tilting AFM, measurements were conducted on a step height standard.

Midas: A Nanotechnological Exploration of Touch

Leonardo ◽  
2009 ◽  
Vol 42 (3) ◽  
pp. 186-192 ◽  
Author(s):  
Paul Thomas
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Atomic Force ◽  
Atomic Vibrations ◽  
Surface Vibrations

The Midas project investigates the trans-mediational space between skin and gold. Research for the project was conducted through the analysis of data recorded with an Atomic Force Microscope (AFM). The AFM, in its force spectroscopy mode, gathers data by picking up the surface vibrations as the cantilever touches the cell. The Midas project culminated in an installation that included data projection and audio work utilizing subsonic speakers to make the data from the atomic vibrations audible and palpable.

Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers

1999 ◽  
Vol 70 (11) ◽  
pp. 4300-4303 ◽  
Author(s):  
M. B. Viani ◽  
T. E. Schäffer ◽  
G. T. Paloczi ◽  
L. I. Pietrasanta ◽  
B. L. Smith ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Force Spectroscopy ◽  
Fast Imaging ◽  
Atomic Force

scholarly journals A Tiny Toolbox

1997 ◽  
Vol 5 (7) ◽  
pp. 3-7
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Atomic Force Microscope ◽  
Single Molecule ◽  
Recent Article ◽  
Single Molecules ◽  
Force Spectroscopy ◽  
Atomic Level ◽  
Single Molecule Force Spectroscopy ◽  
Binding Force ◽  
Atomic Force

A recent article by Matthias Rief, Filipp Oesterhelt. Berthold Heymann, and Hermann Gaub concluded with this sentence: "Single molecule force spectroscopy by AFM has proven to be a powerful addition to the nanoscopic piconewton toolbox," Everything about that conclusion is tiny. Clearly, the atomic force microscope (AFM) has given us a tool to examine structure at or near the atomic level. Earlier work from Gaub's laboratory, reviewed in this column, demonstrated that the AFM could directly measure the binding force between single molecules of biotin and avidin. This established that the AFM could be used as a tool to measure forces, not just observe structure. Their most recent experiments has added to this tiny toolbox.

scholarly journals Investigating pore-forming peptides using the atomic force microscope

2019 ◽  
Author(s):  
◽  
Anna Elizabeth Pittman
Keyword(s):  
Atomic Force Microscope ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Temporal Stability ◽  
Force Spectroscopy ◽  
Hydrodynamic Drag ◽  
Gold Coating ◽  
Cross Sectional ◽  
Lipid Interactions ◽  
Atomic Force

In my projects, I used the Atomic Force Microscope (AFM) to image the effects of pore-forming peptides and I used the Focused Ion Beam (FIB) to modify AFM cantilevers to increase the precision of force spectroscopy measurements. ... Another way to investigate peptide-lipid interactions is to perform force spectroscopy experiments using the AFM. In order to lower drift and increase force precision, I used a FIB to modify commercially available AFM cantilevers. By reducing the cross sectional area of the cantilever, the hydrodynamic drag was reduced, thus increasing the force precision. Removing most of the gold coating on the cantilever increased the temporal stability. These modified cantilevers have already been put to use in the lab to measure peptide-lipid interactions.

ForSDAT: an automated platform for analyzing force spectroscopy measurements

2019 ◽  
Vol 11 (37) ◽  
pp. 4709-4718 ◽  
Author(s):  
Tal Duanis-Assaf ◽  
Yair Razvag ◽  
Meital Reches
Keyword(s):  
Protein Folding ◽  
Data Analysis ◽  
Atomic Force Microscope ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Atomic Force ◽  
Receptor Interactions ◽  
Automated Data Analysis ◽  
Automated Platform

Single molecule force spectroscopy (SMFS) using an atomic force microscope is a common method in the study of ligand–receptor interactions, adhesion events and protein folding. ForSDAT is a platform for automated data analysis of SMFS data.

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