scholarly journals Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

10.3791/55002 ◽  
2016 ◽  
Author(s):  
Jing Men ◽  
Jason Jerwick ◽  
Penghe Wu ◽  
Mingming Chen ◽  
Aneesh Alex ◽  
...  
Keyword(s):  
Heart Function ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Longitudinal Imaging

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

scholarly journals In vivo imaging of corneal nerves and cellular structures in mice with Gabor-domain optical coherence microscopy

2020 ◽  
Vol 11 (2) ◽  
pp. 711 ◽  
Author(s):  
Cristina Canavesi ◽  
Andrea Cogliati ◽  
Amanda Mietus ◽  
Yue Qi ◽  
Jesse Schallek ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Cellular Structures ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Corneal Nerves ◽  
Gabor Domain

scholarly journals Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo

2018 ◽  
Vol 43 (2) ◽  
pp. 198 ◽  
Author(s):  
Conrad William Merkle ◽  
Shau Poh Chong ◽  
Aaron Michael Kho ◽  
Jun Zhu ◽  
Alfredo Dubra ◽  
...  
Keyword(s):  
Visible Light ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
The Brain

In vivo three-dimensional imaging of plants with optical coherence microscopy

2002 ◽  
Vol 208 (3) ◽  
pp. 177-189 ◽  
Author(s):  
A. Reeves ◽  
R. L. Parsons ◽  
J. W. Hettinger ◽  
J. I. Medford
Keyword(s):  
Three Dimensional ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

scholarly journals Micro-Heterogeneity of Flow in a Mouse Model of Chronic Cerebral Hypoperfusion Revealed by Longitudinal Doppler Optical Coherence Tomography and Angiography

2015 ◽  
Vol 35 (10) ◽  
pp. 1552-1560 ◽  
Author(s):  
Vivek J Srinivasan ◽  
Esther Yu ◽  
Harsha Radhakrishnan ◽  
Anil Can ◽  
Mihail Climov ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Capillary Density ◽  
Chronic Cerebral Hypoperfusion ◽  
Cerebral Hypoperfusion ◽  
Oct Angiography ◽  
Imaging Method ◽  
Optical Coherence ◽  
Doppler Optical Coherence Tomography ◽  
Longitudinal Imaging

Although microvascular dysfunction accompanies cognitive decline in aging, vascular dementia, and Alzheimer's disease, tools to study microvasculature longitudinally in vivo are lacking. Here, we use Doppler optical coherence tomography (OCT) and angiography for noninvasive, longitudinal imaging of mice with chronic cerebral hypoperfusion for up to 1 month. In particular, we optimized the OCT angiography method to selectively image red blood cell (RBC)-perfused capillaries, leading to a novel way of assessing capillary supply heterogeneity in vivo. After bilateral common carotid artery stenosis (BCAS), cortical blood flow measured by Doppler OCT dropped to half of baseline throughout the imaged tissue acutely. Microscopic imaging of the capillary bed with OCT angiography further revealed local heterogeneities in cortical flow supply during hypoperfusion. The number of RBC-perfused capillaries decreased, leading to increased oxygen diffusion distances in the days immediately after BCAS. Linear regression showed that RBC-perfused capillary density declined by 0.3% for a drop in flow of 1 mL/100 g per minute, and decreases in RBC-perfused capillary density as high as 25% were observed. Taken together, these results demonstrate the existence of local supply heterogeneity at the capillary level even at nonischemic global flow levels, and demonstrate a novel imaging method to assess this heterogeneity.

Sign in / Sign up

Export Citation Format

Share Document