scholarly journals Staging mouse preimplantation development in vivo using optical coherence microscopy

2019 ◽  
Vol 12 (5) ◽  
Author(s):  
Emma L. Moore ◽  
Shang Wang ◽  
Irina V. Larina
Keyword(s):  
Preimplantation Development ◽  
Optical Coherence ◽  
Optical Coherence Microscopy

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

scholarly journals In vivo imaging of corneal nerves and cellular structures in mice with Gabor-domain optical coherence microscopy

2020 ◽  
Vol 11 (2) ◽  
pp. 711 ◽  
Author(s):  
Cristina Canavesi ◽  
Andrea Cogliati ◽  
Amanda Mietus ◽  
Yue Qi ◽  
Jesse Schallek ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Cellular Structures ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Corneal Nerves ◽  
Gabor Domain

scholarly journals Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo

2018 ◽  
Vol 43 (2) ◽  
pp. 198 ◽  
Author(s):  
Conrad William Merkle ◽  
Shau Poh Chong ◽  
Aaron Michael Kho ◽  
Jun Zhu ◽  
Alfredo Dubra ◽  
...  
Keyword(s):  
Visible Light ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
The Brain

In vivo three-dimensional imaging of plants with optical coherence microscopy

2002 ◽  
Vol 208 (3) ◽  
pp. 177-189 ◽  
Author(s):  
A. Reeves ◽  
R. L. Parsons ◽  
J. W. Hettinger ◽  
J. I. Medford
Keyword(s):  
Three Dimensional ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging
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