scholarly journals In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

10.3791/54576 ◽  
2016 ◽  
Author(s):  
Sara Nullens ◽  
Annemie Deiteren ◽  
Wen Jiang ◽  
Christopher Keating ◽  
Hannah Ceuleers ◽  
...  
Keyword(s):  
Afferent Nerve ◽  
Nerve Activity

scholarly journals A Novel Single Compartment In Vitro Model for Electrophysiological Research Using the Perfluorocarbon FC-770

2016 ◽  
pp. 341-348 ◽  
Author(s):  
M. CHOUDHARY ◽  
F. CLAVICA ◽  
R. VAN MASTRIGT ◽  
E. VAN ASSELT
Keyword(s):  
Afferent Nerve ◽  
Nerve Activity ◽  
Bladder Tissue ◽  
Single Compartment ◽  
Organ Systems ◽  
Electrophysiological Studies ◽  
Spontaneous Contractions ◽  
Stimulation Of

Electrophysiological studies of whole organ systems in vitro often require measurement of nerve activity and/or stimulation of the organ via the associated nerves. Currently two-compartment setups are used for such studies. These setups are complicated and require two fluids in two separate compartments and stretching the nerve across one chamber to the other, which may damage the nerves. We aimed at developing a simple single compartment setup by testing the electrophysiological properties of FC-770 (a perfluorocarbon) for in vitro recording of bladder afferent nerve activity and electrical stimulation of the bladder. Perflurocarbons are especially suitable for such a setup because of their high oxygen carrying capacity and insulating properties. In male Wistar rats, afferent nerve activity was recorded from postganglionic branches of the pelvic nerve in vitro, in situ and in vivo. The bladder was stimulated electrically via the efferent nerves. Organ viability was monitored by recording spontaneous contractions of the bladder. Additionally, histological examinations were done to test the effect of FC-770 on the bladder tissue. Afferent nerve activity was successfully recorded in a total of 11 rats. The bladders were stimulated electrically and high amplitude contractions were evoked. Histological examinations and monitoring of spontaneous contractions showed that FC-770 maintained organ viability and did not cause damage to the tissue. We have shown that FC-770 enables a simple, one compartment in vitro alternative for the generally used two compartment setups for whole organ electrophysiological studies.

Role of peripheral NMDA receptors in colorectal distension mediated afferent nerve activity: An In vitro study

2001 ◽  
Vol 120 (5) ◽  
pp. A329
Author(s):  
Jen Yu Wei ◽  
Yu Hua Wang ◽  
Emeran A. Mayer ◽  
James A. McRoberts
Keyword(s):  
Nmda Receptors ◽  
In Vitro Study ◽  
Afferent Nerve ◽  
Vitro Study ◽  
Nerve Activity ◽  
Colorectal Distension

Atrial ectopic pacemaker escape mediated by phasic vagal nerve activity

1991 ◽  
Vol 260 (5) ◽  
pp. H1507-H1514 ◽  
Author(s):  
G. J. Rozanski
Keyword(s):  
Cycle Length ◽  
Vagal Nerve ◽  
Vagal Activity ◽  
Drive Cycle ◽  
Nerve Activity ◽  
Vagal Nerve Activity ◽  
Ectopic Pacemaker ◽  
Premature Beats ◽  
Spontaneous Discharges

Effects of vagal nerve activity on atrial ectopic pacemaker foci were studied in vitro in strips of rabbit tricuspid valve. Transmembrane potentials were recorded from pacemaker and working atrial fibers superfused with Tyrode solution containing propranolol. Tissues were paced from the atrial muscle end at cycle lengths of 90, 70, or 50% of the intrinsic pacemaker cycle, and postganglionic vagal nerve endings were stimulated with brief trains of pulses (200 Hz; 100-200 microseconds) through a second electrode near the pacemaker. Vagal trains scanning diastole hyperpolarized pacemaker and surrounding fibers to a maximum membrane potential of -74.7 +/- 1.8 mV (normal maximum diastolic potential = -75.5 +/- 1.6 mV) and elicited a period of inexcitability lasting 217.9 +/- 27.3 ms (drive cycle = 90% of pacemaker cycle). Inexcitability was evident at critical diastolic intervals where vagal input prevented atrial impulses from activating the pacemaker allowing spontaneous discharges to occur, i.e., escape, late in diastole. Besides inexcitability, incidence and timing of escape impulses were determined by cumulative effects of drive cycle length, vagal stimulus, and subthreshold electrotonic input on intrinsic pacemaker cycle. These data suggest that phasic vagal stimuli may transiently protect atrial ectopic pacemaker foci from conducted sinus impulses by rendering pacemaker and surrounding fibers inexcitable. In the setting of a long sinus (drive) cycle length, phasic vagal activity may result in spontaneous discharges manifest as late atrial premature beats.

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