scholarly journals The K V 7 channel activator retigabine suppresses mouse urinary bladder afferent nerve activity without affecting detrusor smooth muscle K + channel currents

2018 ◽  
Vol 597 (3) ◽  
pp. 935-950 ◽  
Author(s):  
Nathan R. Tykocki ◽  
Thomas J. Heppner ◽  
Thomas Dalsgaard ◽  
Adrian D. Bonev ◽  
Mark T. Nelson
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Afferent Nerve ◽  
K Channel ◽  
Detrusor Smooth Muscle ◽  
Nerve Activity ◽  
Channel Currents ◽  
Channel Activator

scholarly journals Transient contractions of urinary bladder smooth muscle are drivers of afferent nerve activity during filling

2016 ◽  
Vol 147 (4) ◽  
pp. 323-335 ◽  
Author(s):  
Thomas J. Heppner ◽  
Nathan R. Tykocki ◽  
David Hill-Eubanks ◽  
Mark T. Nelson
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Sensory Nerve ◽  
Bk Channels ◽  
Filling Pressure ◽  
Afferent Nerve ◽  
Detrusor Muscle ◽  
Afferent Activity ◽  
Intravesical Pressure ◽  
Nerve Activity

Activation of afferent nerves during urinary bladder (UB) filling conveys the sensation of UB fullness to the central nervous system (CNS). Although this sensory outflow is presumed to reflect graded increases in pressure associated with filling, UBs also exhibit nonvoiding, transient contractions (TCs) that cause small, rapid increases in intravesical pressure. Here, using an ex vivo mouse bladder preparation, we explored the relative contributions of filling pressure and TC-induced pressure transients to sensory nerve stimulation. Continuous UB filling caused an increase in afferent nerve activity composed of a graded increase in baseline activity and activity associated with increases in intravesical pressure produced by TCs. For each ∼4-mmHg pressure increase, filling pressure increased baseline afferent activity by ∼60 action potentials per second. In contrast, a similar pressure elevation induced by a TC evoked an ∼10-fold greater increase in afferent activity. Filling pressure did not affect TC frequency but did increase the TC rate of rise, reflecting a change in the length-tension relationship of detrusor smooth muscle. The frequency of afferent bursts depended on the TC rate of rise and peaked before maximum pressure. Inhibition of small- and large-conductance Ca2+-activated K+ (SK and BK) channels increased TC amplitude and afferent nerve activity. After inhibiting detrusor muscle contractility, simulating the waveform of a TC by gently compressing the bladder evoked similar increases in afferent activity. Notably, afferent activity elicited by simulated TCs was augmented by SK channel inhibition. Our results show that afferent nerve activity evoked by TCs represents the majority of afferent outflow conveyed to the CNS during UB filling and suggest that the maximum TC rate of rise corresponds to an optimal length-tension relationship for efficient UB contraction. Furthermore, our findings implicate SK channels in controlling the gain of sensory outflow independent of UB contractility.

scholarly journals Dystrophin is expressed in smooth muscle and afferent nerve fibers in the rat urinary bladder

2019 ◽  
Vol 60 (2) ◽  
pp. 202-210 ◽  
Author(s):  
Judith M. Lionarons ◽  
Govert Hoogland ◽  
Ruben G. F. Hendriksen ◽  
Catharina G. Faber ◽  
Danique M. J. Hellebrekers ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Nerve Fibers ◽  
Afferent Nerve ◽  
Rat Urinary Bladder ◽  
Afferent Nerve Fibers
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