scholarly journals Effects of nicotinic receptor agonists on bladder afferent nerve activity in an in vitro bladder–pelvic nerve preparation

2016 ◽  
Vol 1637 ◽  
pp. 91-101 ◽  
Author(s):  
Yongbei Yu ◽  
Stephanie L. Daugherty ◽  
William C. de Groat
Keyword(s):  
Nicotinic Receptor ◽  
Afferent Nerve ◽  
Pelvic Nerve ◽  
Nerve Activity ◽  
Receptor Agonists

scholarly journals In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

10.3791/54576 ◽  
2016 ◽  
Author(s):  
Sara Nullens ◽  
Annemie Deiteren ◽  
Wen Jiang ◽  
Christopher Keating ◽  
Hannah Ceuleers ◽  
...  
Keyword(s):  
Afferent Nerve ◽  
Nerve Activity

scholarly journals A Novel Single Compartment In Vitro Model for Electrophysiological Research Using the Perfluorocarbon FC-770

2016 ◽  
pp. 341-348 ◽  
Author(s):  
M. CHOUDHARY ◽  
F. CLAVICA ◽  
R. VAN MASTRIGT ◽  
E. VAN ASSELT
Keyword(s):  
Afferent Nerve ◽  
Nerve Activity ◽  
Bladder Tissue ◽  
Single Compartment ◽  
Organ Systems ◽  
Electrophysiological Studies ◽  
Spontaneous Contractions ◽  
Stimulation Of

Electrophysiological studies of whole organ systems in vitro often require measurement of nerve activity and/or stimulation of the organ via the associated nerves. Currently two-compartment setups are used for such studies. These setups are complicated and require two fluids in two separate compartments and stretching the nerve across one chamber to the other, which may damage the nerves. We aimed at developing a simple single compartment setup by testing the electrophysiological properties of FC-770 (a perfluorocarbon) for in vitro recording of bladder afferent nerve activity and electrical stimulation of the bladder. Perflurocarbons are especially suitable for such a setup because of their high oxygen carrying capacity and insulating properties. In male Wistar rats, afferent nerve activity was recorded from postganglionic branches of the pelvic nerve in vitro, in situ and in vivo. The bladder was stimulated electrically via the efferent nerves. Organ viability was monitored by recording spontaneous contractions of the bladder. Additionally, histological examinations were done to test the effect of FC-770 on the bladder tissue. Afferent nerve activity was successfully recorded in a total of 11 rats. The bladders were stimulated electrically and high amplitude contractions were evoked. Histological examinations and monitoring of spontaneous contractions showed that FC-770 maintained organ viability and did not cause damage to the tissue. We have shown that FC-770 enables a simple, one compartment in vitro alternative for the generally used two compartment setups for whole organ electrophysiological studies.

Role of peripheral NMDA receptors in colorectal distension mediated afferent nerve activity: An In vitro study

2001 ◽  
Vol 120 (5) ◽  
pp. A329
Author(s):  
Jen Yu Wei ◽  
Yu Hua Wang ◽  
Emeran A. Mayer ◽  
James A. McRoberts
Keyword(s):  
Nmda Receptors ◽  
In Vitro Study ◽  
Afferent Nerve ◽  
Vitro Study ◽  
Nerve Activity ◽  
Colorectal Distension

scholarly journals Modulation of human T-type calcium channels by synthetic cannabinoid receptor agonists in vitro

2021 ◽  
Vol 187 ◽  
pp. 108478
Author(s):  
Chris Bladen ◽  
Somayeh Mirlohi ◽  
Marina Santiago ◽  
Mitchell Longworth ◽  
Michael Kassiou ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Cannabinoid Receptor ◽  
Synthetic Cannabinoid ◽  
Receptor Agonists ◽  
Synthetic Cannabinoid Receptor Agonists

scholarly journals Engineered Stem Cells Improve Neurogenic Bladder by Overexpressing SDF-1 in a Pelvic Nerve Injury Rat Model

2020 ◽  
Vol 29 ◽  
pp. 096368972090246 ◽  
Author(s):  
Guan Qun Zhu ◽  
Seung Hwan Jeon ◽  
Kyu Won Lee ◽  
Hyuk Jin Cho ◽  
U-Syn Ha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Neurogenic Bladder ◽  
Rat Model ◽  
Bladder Wall ◽  
Bladder Function ◽  
Pelvic Nerve ◽  
Injured Nerve

There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marrow mesenchymal stem cells (BM-MSCs) were transfected into immortalized upregulated SDF-1-engineered BM-MSCs (imMSCs/eSDF-1+) or immortalized normal SDF-1-engineered BM-MSCs (imMSCs/eSDF-1−). NB rats induced by bilateral pelvic nerve (PN) transection were treated with imMSCs/eSDF-1+, imMSCs/eSDF-1−, or sham. After a 4-week treatment, the bladder function was assessed by cystometry and voiding pattern analysis. The PN and bladder tissues were evaluated via immunostaining and western blotting analysis. We found that imMSCs/eSDF-1+ expressed higher levels of SDF-1 in vitro and in vivo. The treatment of imMSCs/eSDF-1+ improved NB and evidently stimulated the recovery of bladder wall in NB rats. The recovery of injured nerve was more effective in the NB+imMSCs/eSDF-1+ group than in other groups. High SDF-1 expression improved the levels of vascular endothelial growth factor and basic fibroblast growth factor. Apoptosis was decreased after imMSCs injection, and was detected rarely in the NB+imMSCs/eSDF-1+ group. Injection of imMSCs boosted the expression of neuronal nitric oxide synthase, p-AKT, and p-ERK in the NB+imMSCs/eSDF-1+ group than in other groups. Our findings demonstrated that overexpression of SDF-1 induced additional MSC homing to the injured tissue, which improved the NB by accelerating the restoration of injured nerve in a rat model.

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