Determination of In vitro Anti-diabetic and Antioxidant Enzyme Potentials of leaf Extract of Acanthus ilicifolius -Mangrove plant

2020 ◽  
Vol 7 (10) ◽  
Keyword(s):  
Antioxidant Enzyme ◽  
Leaf Extract ◽  
Mangrove Plant ◽  
Acanthus Ilicifolius

scholarly journals Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant)

2020 ◽  
Vol 25 (2) ◽  
pp. 94
Author(s):  
Sesilia Rante Pakadang ◽  
Santi Sinala ◽  
Alfrida Monica Salasa ◽  
St Ratnah ◽  
Sisilia Teresia Rosmala Dewi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Effective Dose ◽  
Leaf Extract ◽  
Sputum Sample ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Enterobacter Agglomerans ◽  
Dpph Method

Research has been conducted on the treatment of phlegm cough with miana leaf extract in vitro (effective dose of miana leaf extract as an expectorant and antibacterial agent causing cough with phlegm). The study aims to compare the antioxidant activity of miana leaves from 3 locations where it grows, determine the types of contaminant bacteria in the sputum of cough sufferers, determine the minimum value of inhibitor concentration (MIC) and MKC of miana leaves against the test bacteria causing cough with phlegm, determine the effective dose of miana leaves that can used as a reference for coughing up phlegm and proving the potential of miana leaves as a sputum thinner. Miana leaf extraction is done by the juicer method. Antioxidant activity testing uses the DPPH method. Determination of test bacteria is done by isolating and identifying contaminant bacteria in the sputum sample of cough with phlegm. Testing the effectiveness of miana leaves against test bacteria is determined by the liquid dilution method. Expectorant activity testing was determined by measuring the viscosity of mucus viscosity of cow intestine treated with miana leaf extract. The results found that antioxidant activity was influenced by the location where miana leaves grew with an antioxidant potential of IC72 0.072 mg/ml - 0.76 mg/ml. Contaminant bacteria from sputum samples of cough patients are Streptococcus pneumonia, Klebsiella pneumonia, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter agglomerans. MIC values for contaminant bacteria are 0.1% - 0.75% and MKC values are found between 0.25% - 1.75%. miana leaf extract has the potential as a sputum thinner at a concentration of 0.01% - 0.1%. The recommended dose of miana leaf extract as a cough with phlegm is 1.75% w/v.

scholarly journals Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)

2021 ◽  
Vol 9 (4) ◽  
pp. 814-817
Author(s):  
İlter Demirhan ◽  
Büşra Çitil ◽  
Mehmet Özyurt ◽  
Meltem Güngör ◽  
Erkan Öner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Quercus Infectoria

South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.

scholarly journals In Vitro Determination of SunProtective Factor (SPF) of Dadap Serep (Erythrina Subumbrans (Haks.) Merr.) Leaf Extract Using Spectrophotometric Method

2019 ◽  
Vol 1 (1) ◽  
pp. 64-67
Author(s):  
Indri Maharini
Keyword(s):  
Bioactive Compounds ◽  
Spectrophotometric Method ◽  
Leaf Extract ◽  
Protective Factor ◽  
Ethanol Extract ◽  
Flavonoid Compounds

Dadap serep (Erythrina Subumbrans Haks Merr) contains bioactive compounds of flavonoids, saponins, isoflavones, alkaloids, and lectins. Flavonoid compounds have a sunscreen effect. This studyaimed to determinethe value of Sun Protective Factor (SPF) using spectrophotometry method with variations in the extract concentrations, such as500 ppm,750 ppm, and 1000 ppm.The results showed that the SPF values of each concentration were11.47 ±0.583335, 17.05 ± 0.898838,21.93 ± 0.791557, respectively. In conclusion, the ethanol extract of dadap serep hastheability to become sunscreen in the maximal and ultra category.

In vitro antioxidant potential and in vivo effects of Schinus terebinthifolia Raddi leaf extract in diabetic rats and determination of chemical composition by HPLC-ESI-MS/MS

2018 ◽  
Vol 33 (11) ◽  
pp. 1655-1658 ◽  
Author(s):  
Camila Cristina Iwanaga ◽  
Lilian dos Anjos Oliveira Ferreira ◽  
Karine Zanoli Bernuci ◽  
Carla Maria Mariano Fernandez ◽  
Fabiana Brusco Lorenzetti ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Antioxidant Potential ◽  
Esi Ms ◽  
In Vivo Effects

scholarly journals In vitro Determination of Fungicide Inhibitory Concentration for Phakopsora pachyrhizi isolates

2016 ◽  
Vol 42 (2) ◽  
pp. 170-171 ◽  
Author(s):  
Bianca Moura ◽  
Walter Boller ◽  
Carolina Cardoso Deuner
Keyword(s):  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Agar Medium ◽  
In Vitro Assays ◽  
Phakopsora Pachyrhizi ◽  
Ic50 Values ◽  
Soybean Leaf ◽  
Petri Dishes

ABSTRACT In vitro assays were preformed to obtain the IC50 of eight fungicides against Phakopsora pachyrhizi isolates from Passo Fundo, RS, Ponta Grossa, PR, and Primavera do Leste, MT. Different concentrations of the fungicides were added to Petri dishes containing soybean leaf extract agar medium. One milliliter of P. pachyrhizi uredospore suspension at the concentration of 3.0 x 104 uredospores/mL was added to each dish for subsequent viability quantification. Only pyraclostrobin and the mixture trifloxystrobin + prothioconazole showed IC50 values inferior to 1.0 mg/L for all tested isolates, demonstrating high fungitoxicity. There was not loss of sensitivity to any of the tested fungicides.

Sign in / Sign up

Export Citation Format

Share Document