scholarly journals New tridecapeptides of the theonellapeptolide family from the Indonesian sponge Theonella swinhoei

2013 ◽  
Vol 9 ◽  
pp. 1643-1651 ◽  
Author(s):  
Annamaria Sinisi ◽  
Barbara Calcinai ◽  
Carlo Cerrano ◽  
Henny A Dien ◽  
Angela Zampella ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
Hepg2 Cells ◽  
Carcinoma Cell ◽  
2D Nmr ◽  
Carcinoma Cell Line ◽  
Hepatic Carcinoma ◽  
Organic Extract ◽  
Theonella Swinhoei

Chemical analysis of the organic extract of Theonella swinhoei yielded two new tridecadepsipeptides of the theonellapeptolide family, namely sulfinyltheonellapeptolide, characterized by a methylsulfinylacetyl group at the N-terminus, and theonellapeptolide If, the first member of this class of compounds to show four valine residues. The structures of the compounds, isolated along with the known theonellapeptolide Id, were determined by extensive 2D NMR and MS/MS analyses followed by application of Marfey’s method. The isolated peptides exhibited moderate antiproliferative activity against HepG2 cells, a hepatic carcinoma cell line.

scholarly journals The 3-D Culture andIn VivoGrowth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Min Wu ◽  
Zehong Yang ◽  
Yanfei Liu ◽  
Bo Liu ◽  
Xiaojun Zhao
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cells ◽  
Carcinoma Cell ◽  
Human Hepatocellular Carcinoma ◽  
Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell Line ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Carcinoma Cell Line Hepg2

We report the use of the RADA16-I scaffold to mimic the ECM microenvironment and support tumor cell adherence and survival. Cellular morphology, proliferation, adhesion ability, andin vivotumor formation were studied in the human hepatocellular carcinoma cell line HepG2 in the 3-D RADA16-I scaffold. No significant differences in HepG2 cell proliferation, adhesion, and albumin secretion were observed in the peptide scaffold compared to collagen I. Furthermore, the HepG2 cells precultured in the peptide scaffold showed a higher proliferation rate and formed significantly bigger tumors when compared to cells grown on a traditional 2D monolayer, suggesting that the 3-D RADA16-I scaffold can mimic the tumor microenvironment and promote a malignant phenotype in HepG2 cells. Our results indicate that the RADA16-I scaffold can serve as an ideal model for tumorigenesis, growth, local invasion, and metastasis.

Antiproliferative Activity, Cell Cycle, and Apoptosis Studies of a Series of 6-Substituted 9H-Purin-9-yl-pyridinium Derivatives on a Human Cervical Carcinoma Cell Line

ChemMedChem ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. 1266-1269 ◽  
Author(s):  
Belén Rubio-Ruiz ◽  
Javier Ramos-Torrecillas ◽  
Fermín Capitán-Cañadas ◽  
Rosario Sánchez-Martín ◽  
Miguel Ángel Gallo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Cervical Carcinoma ◽  
Antiproliferative Activity ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Human Cervical Carcinoma ◽  
Cervical Carcinoma Cell Line

scholarly journals Cytotoxic Effects of Hydro-Alcoholic Extract of the Leaf of Elaeagnus angustifolia in Hepatocellular Carcinoma Cell Line (HepG2)

2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Manijeh Ghanghareh ◽  
Maryam Zare
Keyword(s):  
Side Effects ◽  
Redox Potential ◽  
Cell Line ◽  
Hepg2 Cells ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
New Drugs ◽  
Liver Carcinoma ◽  
Alcoholic Extract ◽  
Carcinoma Cell Line Hepg2

Background: Cancer is one of the most complicated diseases with various treatments, which each has its special side effects. So, pharmaceutical companies are intended to develop new drugs with minimum side effects. Objectives: The current study aimed to investigate the cytotoxicity effects and the redox potential of alcoholic extract of Oleaster leaf on liver carcinoma cell line (HepG2). Methods: Oleaster leaves were collected from Qazvin (Iran), and the alcoholic extract of the plant leaves was prepared. HepG2 cells were cultured in DMEM medium and treated with 50, 100, 200, 400, and 600 μg/mL of the extract. The cytotoxicity effect of the extract was evaluated using the MTT and the Neutral Red assays. Redox potential in HepG2 cells was assessed using NO, catalase, and GSH tests. The expression of Bax and bcl-2 genes in HepG2 cells was evaluated for apoptosis analysis. Results: The results showed that the extract could significantly (P < 0.001) reduce the viability of HepG2 cells. Also, the extract significantly increased the amount of released NO, catalase activity, and GSH concentration. RT-PCR results showed that Oleaster leaf extract significantly change the expression of bax and bcl-2. Conclusions: The results showed that the leaves of the Oleaster plant contain compounds with cytotoxicity properties, so it can be considered as a potent candidate for liver cancer treatment.

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