Enzyme-linked immunosorbent assay to measure apolipoproteins Al and B secreted by a human hepatic carcinoma cell line (Hep G2)

1992 ◽  
Vol 6 (4) ◽  
pp. 182-189 ◽  
Author(s):  
Susan E. Hahn ◽  
Joel G. Parkes ◽  
David M. Goldberg
Keyword(s):  
Cell Line ◽  
Immunosorbent Assay ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hep G2 ◽  
Hepatic Carcinoma

scholarly journals New tridecapeptides of the theonellapeptolide family from the Indonesian sponge Theonella swinhoei

2013 ◽  
Vol 9 ◽  
pp. 1643-1651 ◽  
Author(s):  
Annamaria Sinisi ◽  
Barbara Calcinai ◽  
Carlo Cerrano ◽  
Henny A Dien ◽  
Angela Zampella ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
Hepg2 Cells ◽  
Carcinoma Cell ◽  
2D Nmr ◽  
Carcinoma Cell Line ◽  
Hepatic Carcinoma ◽  
Organic Extract ◽  
Theonella Swinhoei

Chemical analysis of the organic extract of Theonella swinhoei yielded two new tridecadepsipeptides of the theonellapeptolide family, namely sulfinyltheonellapeptolide, characterized by a methylsulfinylacetyl group at the N-terminus, and theonellapeptolide If, the first member of this class of compounds to show four valine residues. The structures of the compounds, isolated along with the known theonellapeptolide Id, were determined by extensive 2D NMR and MS/MS analyses followed by application of Marfey’s method. The isolated peptides exhibited moderate antiproliferative activity against HepG2 cells, a hepatic carcinoma cell line.

QUANTIFICATION OF ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) ANTIGEN BY AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)

1987 ◽  
Author(s):  
I R MacGregor ◽  
N A Booth ◽  
N R Hunter ◽  
B Bennet
Keyword(s):  
Cell Line ◽  
Carcinoma Cell ◽  
Specific Activity ◽  
Carcinoma Cell Line ◽  
Rabbit Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Breast Carcinoma Cell Line ◽  
Standard Samples ◽  
Wide Range ◽  
Pai 1

An ELISA to measure PAI-1 antigen has been developed using PAI-1 purified from human endothelial cell conditioned medium and a monospecific antiserum raised against it in the rabbit. Test and standard samples diluted in assay buffer containing non-immune rabbit serum were incubated in microplate wells coated with anti-PAI-1 IgG. Then biotinylated anti-PAI-1 IgG was added to the wells followed by a streptavidin-biotinylated horseradish peroxidase complex. Tetramethylbenzidine was used as substrate and the optimised ELISA had a detection limit of 0.2 ng PAI-1 ml-1 sample, using purified PAI-1 of known concentration as a standard for the assay.PAI-1 antigen was readily detectable in human plasmas and higher concentrations were invariably detected in the corresponding whole blood sera.α2antiplasmin and antithrombin III which have been shown to have high degrees of sequence homology with PAI-J were undetectable in the ELISA at concentrations of 10 ug ml-1 . Sera from baboon and Rhesus and Cynomologus monkeys exhibited partial cross reactivity in the ELISA while no crossreactivities were observed with a wide range of non-primate sera that included dog, goat, cow, horse, pig and rat.A variety of human cell cultures were assayed for PAI-1 antigen. Endothelial cells from umbilical cord veins and arteries and from adult sapgenous veins secreted, typically,1-2 ug PAI-1 24-1 hr per 10 cells. This represented approximately 2-4% of the total secreted protein. Detectable but considerably lower levels of PAI-1 were secreted by keratinocytes and fibroblasts as well as a lung carcinoma cell line A549.No PAI-1 was detected in media conditioned by two melanoma cell lines, a breast carcinoma cell line MCF7 or a monocytic leukaemia JIII. The ELISA proved suitable for monitoring altered cellular PAI-1 metabolism, and in conjunction with functional assays, for investigating changes in PAI-1 specific activity.

520: Establishment of an Adrenocortical Carcinoma Cell Line (SO-RY), and its Characterization Including Adrenal Cortical Steroidogenesis

2005 ◽  
Vol 173 (4S) ◽  
pp. 141-142
Author(s):  
Yutaka Kamiyama ◽  
Hiroshi Okada ◽  
Koujiro Nishio ◽  
Takashi Yoshii ◽  
Keisuke Saito ◽  
...  
Keyword(s):  
Cell Line ◽  
Adrenocortical Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line
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