scholarly journals Efektifitas Pemberian Minyak Zaitun Ekstra Virgin Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Dengan Induksi Hiperglikemia

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Sri Lestari Dwi Astuti ◽  
Yeni Tutu Rohimah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Olive Oil ◽  
Glucose Level ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Standard Diet

Background: The global population of diabetes mellitus tends to increase every year. This is related to the increased of population numbers, lifestyle, increased prevalence of obesity and less physical activity (Smeltzer & Bare, 2002). Olive oil is a functional diet because it is rich of monounsaturated fatty acids (MUFA), especially oleic acid (70% - 80%), contains antioxidants and polyphenol compounds. Polyphenols are α-amylase enzyme inhibitors that have function in the breakdown of carbohydrates, so that the level of blood glucose in hyperglycemia can be lowered (Astrawan made, Wresdiyati Tutik, Nasution Nurlayla Amas, 2015). The goal of this study is to examine the effectiveness of extra virgin olive oil to blood glucose level. Method:This study used experimental laboratory study with post test only control group design. The independent variable was the extra virgin olive oil, while the dependent variable was the blood glucose level. The samples were 32 healthy 8 weeks old white male wistar strain rats with the weigh between 180 mg - 200 mg. It divided into 3 groups, the negative control group was fed with standard diet, the positive control group was fed with standard diet and 1 ml fructose for 4 weeks, the treatment group was fed with standard diet, 1 ml fructose and 0.5 gr extra virgin olive oil for 4 weeks, on the fifth week the fructose was increased into 4 ml/day for the next 4 weeks, on the 52nd day the blood glucose level was checked after it was fasted for 6 hours. The data analysis used t-test and Mann Withney test with α = 0.05. Result: The blood glucose level average of the positive control group was 309.57 ± 120.12, the blood glucose level average of the negative control group was 111.85 ± 10.57, the blood glucose level average of the treatment group was 115.12 ± 12.25. Conclusion: Giving the extra virgin olive oil can effectively lower the blood glucose level on the male wistar rats.

scholarly journals Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Intan Fitri Aprila ◽  
M. Nur Salim ◽  
Razali Daud ◽  
T. Armansyah ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Mus Musculus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

scholarly journals Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

2018 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Eldaa Putik Bunga Melati ◽  
Budiyanti Wiboworini ◽  
Briandani Subariyanti
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Rattus Norvegicus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Rhizopus Sp

<p><strong><em>Introductions : </em></strong><em>Control</em><em>l</em><em>ing of blood glucose would be important </em><em>to prevent </em><em>many complications</em><em> of </em><em>DM.</em><em> Consuming fiber is one</em><em> way to maintain blood glucose </em><em>level</em><em>. These amount of fiber could be obtained in tofu fermented by <span style="text-decoration: underline;">Rhizopus sp</span>. This research aimed to know the effect of tofu fermented by <span style="text-decoration: underline;">Rhizopus sp.</span></em><em> in controlling </em><em> blood glucose level of diabetic induced white rats (<span style="text-decoration: underline;">Rattus norvegicus</span>).</em></p><p><strong><em>Methods : </em></strong><em>This research was a laboratoric-experimental study. The subjects were <span style="text-decoration: underline;">Rattus norvegicus</span>grouped randomly. The total 30 <span style="text-decoration: underline;">Rattus norvegicus</span> were injected by Alloxan 15mg/100gramBW, then</em><em> randomly allocated</em><em> into 5 group, positive control group (glibenclamide 0</em><em>.</em><em>05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (</em><em>0.025gram/100 gramB</em><em>W</em><em>/</em><em>day; </em><em>0.050gram/100 gramBW/</em><em>day; </em><em>0.100gram/100gramBW/</em><em>day). The</em><em> research</em><em> design was </em><em>Pretest and Posttest Control Group Design</em><em>. The</em><em> fasting blood glucose were measured</em><em>before Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).</em></p><p><strong><em>Result</em></strong><strong><em>s</em></strong><strong><em> : </em></strong><em>there was</em><em> a </em><em>significant decreas</em><em>e</em><em> of blood glucose level in all treatment groups and positive control (p = 0</em><em>.</em><em>001). Post Hoc Test showed </em><em>a</em><em> significant difference between all treatment groups and positive control group compared to negative control group (p = 0</em><em>.</em><em>001).</em></p><p><strong><em>Conclusion :</em></strong><em>Tofu fermented by <span style="text-decoration: underline;">Rhizopus sp</span>. was able to decrease blood glucose level in diabetic rats (<span style="text-decoration: underline;">Rattus norvegicus</span>).</em></p><p><em> </em></p><p><strong><em>Keyword </em></strong><em>: Tofu fermented</em><em>, </em><em><span style="text-decoration: underline;">Rhizopus sp</span></em><em><span style="text-decoration: underline;">.</span></em><em>, blood glucose level, alloxan, glibenclamide</em><strong></strong></p>

scholarly journals PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculata) TERHADAP KADAR GLUKOSA DARAH MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN (Effect of Long Bean (Vigna unguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Intan Fitri Aprila ◽  
M. Nur Salim ◽  
Razali Daud ◽  
T. Armansyah ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Vigna Unguiculata ◽  
Mus Musculus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

scholarly journals Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

2020 ◽  
Vol 8 (02) ◽  
pp. 35-39
Author(s):  
Laxmi Shrestha ◽  
Amit Shrivastava ◽  
Bishal Joshi ◽  
Buddhi Raj Pokhrel ◽  
Shanti Gurung ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Aqueous Extract ◽  
Nigella Sativa ◽  
Negative Control ◽  
Hypoglycemic Effect ◽  
Control Group ◽  
Oral Glucose ◽  
Standard Group

INTRODUCTION Diabetes mellitus is the most common metabolic disease worldwide. Nigella sativa also known as black cumin, is an emerging miracle herb with a rich historical and religious background. In the present study, we have attempted to study the hypoglycemic effect of aqueous extract of Nigella sativa on diabetes-induced rats. MATERIAL AND METHODS This preclinical study was conducted in Department of Pharmacology from August to October 2019. In this study, hyperglycemia was induced by a single intraperitoneal dose of 60 mg/kg streptozotocin in 0.1 M citrate buffer (pH 4.5). Animals were divided into five groups containing normal control, negative control, standard, test-1, and test-2. The aqueous extract of Nigella sativa was administered 400 and 800 mg/kg per oral in both the test groups for 18 days. Blood glucose level was measured at three-days interval by glucometer. Oral glucose tolerance test was done by administrating 2 gm/kg per oral glucose and the blood glucose was recorded every two-hours interval for eight hours. RESULTS An oral glucose tolerance test showed a decrease in blood glucose level at the dose of 400 and 800 mg/kg compared to the negative control group. The administration of aqueous extract of Nigella sativa for 18 days showed significant decrease in blood glucose level (p=0.001) compared to negative control group. The blood glucose level in test 1 (400 mg/kg) was significantly reduced compared to the standard group (p***<0.001). The blood glucose level in test 2 at 18th day was less significant (p*< 0.05) compared with standard group and test 1 (p**<0.01, p***<0.001). CONCLUSION The findings of the present study indicate that the aqueous extract of Nigella sativa has significant hypoglycemic effect in Wistar rats.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 44
Author(s):  
Valentina Verrel Purnomo ◽  
Agustinus Sareh Arjono Tjandra ◽  
Risma Risma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
White Male ◽  
Test Method ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Standard Diet ◽  
Malus Sylvestris

<p>Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to control the increase of glicemic index. Apple has antioxidant substance such as quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.</p><p>            This research aims to reveal the effect of Manalagi apple vinegar (<em>Malus sylvestris </em>Mill) towards blood glucose level of alloxan induced Wistar white male rats (<em>Rattus norvegicus</em>).</p><p>            This research is experimental laboratory using <em>Experimental </em>methods. The samples are 30 white rats divided into three groups for 31 days, 1) group which only given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for 14 days. The measurement of blood serum glucose level is done on day twenty four using homogenous colorimetric enzymatic test method for all groups.</p><p>            Blood glucose average in group with only given standard diet as negative control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of <em>One-Way Anova </em>significance<em> </em>is 0.229.</p><p>            The conclusion of this research is that Manalagi apple vinegar treatment doesn’t able to decrease the blood glucose level of white Wistar male rats significantly.</p><p> </p><strong>Keywords </strong>: <em>Malus sylvestris </em>Mill, Blood glucose, Alloxan.

scholarly journals Pengaruh Pemberian Sagu (Metroxylon spp) terhadap Kadar Glukosa Darah Mencit Putih Jantan (Mus musculus) Diabetes Melitus

2018 ◽  
Vol 8 (2) ◽  
pp. 193
Author(s):  
Wilda Laila
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Mus Musculus ◽  
Rice Flour ◽  
Control Group ◽  
P Value ◽  
Standard Diet ◽  
Significant Difference

Diabetes Mellitus (DM) causes various complications if it is not controlled properly. International Diabetes Federation (IDF, 2015) stated that the prevalence DM in 2014 were 8, 3% or equals to 387 million in the world. The objective of this research was to identify the influence of Sago (Metroxylon spp) on blood glucose levels of male white mouse (Mus musculus) with Diabetes Mellitus. This research was an experimental research with pre-test and post-test only group design, conducted from October to December 2016. The samples were twentyfive male white mice (Mus musculus) what were divided into five groups such as : negative control group was given standard diet, the positive control group was given induction of alloxan, three treatment groups (P1) 65mg/20g BW/day was given rice flour, groups (P2) 65mg/20g BW/day was given sago and P3 group was given sago 130mg/20g BW/day. The data were analyzed by Anova testing and Post Host Test testing with trust degree of 95%. The result was the blood glucose level of the mice showed that there was a significant difference with p value 0,012 on group (P2) with treatment, while in group treatment (P3), there was also significant difference with p value 0,019. It could be concluded that there was a significant difference in the average blood glucose level after the rice flour and sago treatment towards various groups.  Keyword : Sago, Rice Flour, Blood Glucose Level, and Diabetes Mellitus

scholarly journals PENGARUH EKSTRAK ETANOLIK BUAH PARIJOTO (Medinilla speciosa Blume) TERHADAP KADAR GULA DARAH DAN FUNGSI SEKSUAL TIKUS JANTAN GALUR WISTAR MODEL DIABETES MELLITUS KRONIK

2018 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Rina Wijayanti ◽  
Asih Puji Lestari
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Sexual Function ◽  
Glucose Level ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Negative Group ◽  
One Way Anova

ABSTRACTChronic diabetes mellitus is a disease of increased blood glucose levels in a long time so that it can affect sexual function such as damage spermatogenesis and aphrodisiac function. Parijoto has flavonoid content. This study aimed to determine the effect of ethanolic extract of parijoto (Medinilla speciosa Blume) fruit in blood glucose level and the sexual function in alloxan-induced diabetic male rats. The design of the study was post test only control group design, 25 male wistar rats aged 2 months with BB 100-200 gram divided into 5 groups. Group 1 (normal control), group 2 (negative control) induced alloxan 100mg / kgBB, groups 3, 4, and 5 were alloxan induced and administered EEBP at doses of 100 mg / kgBW, 250 mg / kgBW, and 500 mg / kgBW. Alloxan-induced rats were left for 4 weeks to achieve chronic DM. Data analysis of blood glucose level and spermatozoa quality using one way anova followed by LSD post hoc. While aphrodisiac of introducing and climbing using one way anova continued by LSD and coitus using kruskal wallis. The results showed that there were significant differences between negative control with normal group, III, IV, and V. The effect of ethanolic extract of parijoto fruit on blood glucose level and sexual function in alloxan-induced diabetic male rats showed no difference between treatment group of 100 mg / kgBW with negative group, and there were significant differences between treatment group of 250 mg / kgBW and 500 mg / kgBW with negative group. Ethanolic extract of parijoto fruit at dose 250 mg / kgBB and 500 mg / kgBB decrease blood glucose level, improve and increase sexual function in alloxan-induced diabetic male rats.

scholarly journals Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

2017 ◽  
Vol 9 (3) ◽  
pp. 312-317
Author(s):  
KARTIAWATI ALIPIN ◽  
EMA PURNAMA SARI ◽  
MADIHAH MADIHAH ◽  
TIA SETIAWATI ◽  
NINING RATNINGSIH ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Kidney Damage ◽  
Diabetic Rat ◽  
Control Group ◽  
Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level ≥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

scholarly journals PENGARUH SINBIOTIK KEFIR TEPUNG PISANG BATU (Musa balbisiana) TERHADAP KADAR GLUKOSA DARAH TIKUS SINDROM METABOLIK

2020 ◽  
Vol 7 (1) ◽  
pp. 18-27
Author(s):  
Dhea Marliana Salsabila ◽  
Taufik Maryusman ◽  
Iin Fatmawati
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Negative Control ◽  
Standard Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Musa Balbisiana ◽  
Significant Difference

Metabolic syndrome begins with insulin resistance characterized by hyperglycemia. Synbiotic kefir banana (Musa balbisiana Colla) flour reduces blood glucose level. This study was conducted to analyze the effects of synbiotic kefir banana (M. balbisiana) flour on blood glucose level of metabolic syndrome rats. This study used 24 Sprague Dawley.rats which were divided into four groups, i.e. negative control was given standard diet, positive control was given high fat fructose diet (HFFD), treatment I (PI) and treatment II (PII) were given HFFD and synbiotic kefir banana (M. balbisiana) flour 1.8 mL 200 g־¹ rat BW per day (PI) and 3.6 mL 200 g־¹ rat BW per day (PII), respectively, for three weeks. The result showed a significant difference (p=0.000) in blood glucose after giving synbiotic kefir banana (M. balbisiana) flour. Synbiotic kefir banana (M. balbisiana) flour reduced blood glucose level in metabolic syndrome rats.Keywords: banana flour, blood glucose level, kefir, metabolic syndrome, synbiotic ABSTRAKSindrom metabolik diawali resistensi insulin yang ditandai hiperglikemia. Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah. Penelitian ini dilakukan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (M. balbisiana) terhadap kadar glukosa darah tikus sindrom metabolik. Penelitian ini menggunakan 24 tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif yang diberi pakan standar, kontrol positif yang diberi high fat fructose diet (HFFD), dan perlakuan I (PI) serta perlakuan II (PII) yang masing-masing diberi HFFD dan sinbiotik kefir tepung pisang batu (M. balbisiana) 1,8 mL 200 g־¹ BB tikus per hari (PI) dan 3,6 mL 200 g־¹ BB tikus per hari (PII) selama tiga minggu. Hasil menunjukkan perbedaan kadar glukosa darah setelah pemberian sinbiotik kefir tepung pisang batu (M. balbisiana) secara signifikan (p=0,000). Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah tikus sindrom metabolik.

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