scholarly journals Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 44
Author(s):  
Valentina Verrel Purnomo ◽  
Agustinus Sareh Arjono Tjandra ◽  
Risma Risma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
White Male ◽  
Test Method ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Standard Diet ◽  
Malus Sylvestris

<p>Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to control the increase of glicemic index. Apple has antioxidant substance such as quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.</p><p>            This research aims to reveal the effect of Manalagi apple vinegar (<em>Malus sylvestris </em>Mill) towards blood glucose level of alloxan induced Wistar white male rats (<em>Rattus norvegicus</em>).</p><p>            This research is experimental laboratory using <em>Experimental </em>methods. The samples are 30 white rats divided into three groups for 31 days, 1) group which only given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for 14 days. The measurement of blood serum glucose level is done on day twenty four using homogenous colorimetric enzymatic test method for all groups.</p><p>            Blood glucose average in group with only given standard diet as negative control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of <em>One-Way Anova </em>significance<em> </em>is 0.229.</p><p>            The conclusion of this research is that Manalagi apple vinegar treatment doesn’t able to decrease the blood glucose level of white Wistar male rats significantly.</p><p> </p><strong>Keywords </strong>: <em>Malus sylvestris </em>Mill, Blood glucose, Alloxan.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Pengaruh Pemberian Sagu (Metroxylon spp) terhadap Kadar Glukosa Darah Mencit Putih Jantan (Mus musculus) Diabetes Melitus

2018 ◽  
Vol 8 (2) ◽  
pp. 193
Author(s):  
Wilda Laila
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Mus Musculus ◽  
Rice Flour ◽  
Control Group ◽  
P Value ◽  
Standard Diet ◽  
Significant Difference

Diabetes Mellitus (DM) causes various complications if it is not controlled properly. International Diabetes Federation (IDF, 2015) stated that the prevalence DM in 2014 were 8, 3% or equals to 387 million in the world. The objective of this research was to identify the influence of Sago (Metroxylon spp) on blood glucose levels of male white mouse (Mus musculus) with Diabetes Mellitus. This research was an experimental research with pre-test and post-test only group design, conducted from October to December 2016. The samples were twentyfive male white mice (Mus musculus) what were divided into five groups such as : negative control group was given standard diet, the positive control group was given induction of alloxan, three treatment groups (P1) 65mg/20g BW/day was given rice flour, groups (P2) 65mg/20g BW/day was given sago and P3 group was given sago 130mg/20g BW/day. The data were analyzed by Anova testing and Post Host Test testing with trust degree of 95%. The result was the blood glucose level of the mice showed that there was a significant difference with p value 0,012 on group (P2) with treatment, while in group treatment (P3), there was also significant difference with p value 0,019. It could be concluded that there was a significant difference in the average blood glucose level after the rice flour and sago treatment towards various groups.  Keyword : Sago, Rice Flour, Blood Glucose Level, and Diabetes Mellitus

scholarly journals PENGARUH EKSTRAK ETANOLIK BUAH PARIJOTO (Medinilla speciosa Blume) TERHADAP KADAR GULA DARAH DAN FUNGSI SEKSUAL TIKUS JANTAN GALUR WISTAR MODEL DIABETES MELLITUS KRONIK

2018 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Rina Wijayanti ◽  
Asih Puji Lestari
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Sexual Function ◽  
Glucose Level ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Negative Group ◽  
One Way Anova

ABSTRACTChronic diabetes mellitus is a disease of increased blood glucose levels in a long time so that it can affect sexual function such as damage spermatogenesis and aphrodisiac function. Parijoto has flavonoid content. This study aimed to determine the effect of ethanolic extract of parijoto (Medinilla speciosa Blume) fruit in blood glucose level and the sexual function in alloxan-induced diabetic male rats. The design of the study was post test only control group design, 25 male wistar rats aged 2 months with BB 100-200 gram divided into 5 groups. Group 1 (normal control), group 2 (negative control) induced alloxan 100mg / kgBB, groups 3, 4, and 5 were alloxan induced and administered EEBP at doses of 100 mg / kgBW, 250 mg / kgBW, and 500 mg / kgBW. Alloxan-induced rats were left for 4 weeks to achieve chronic DM. Data analysis of blood glucose level and spermatozoa quality using one way anova followed by LSD post hoc. While aphrodisiac of introducing and climbing using one way anova continued by LSD and coitus using kruskal wallis. The results showed that there were significant differences between negative control with normal group, III, IV, and V. The effect of ethanolic extract of parijoto fruit on blood glucose level and sexual function in alloxan-induced diabetic male rats showed no difference between treatment group of 100 mg / kgBW with negative group, and there were significant differences between treatment group of 250 mg / kgBW and 500 mg / kgBW with negative group. Ethanolic extract of parijoto fruit at dose 250 mg / kgBB and 500 mg / kgBB decrease blood glucose level, improve and increase sexual function in alloxan-induced diabetic male rats.

scholarly journals PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN

2019 ◽  
Vol 20 (2) ◽  
pp. 70-75
Author(s):  
Yuliyanti Yuliyanti ◽  
A. Sareh Arjono Tjandra ◽  
Herin Setianingsih
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Malus Sylvestris ◽  
Wistar Strain ◽  
Significant Difference

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects.  Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties

scholarly journals Efektifitas Pemberian Minyak Zaitun Ekstra Virgin Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Dengan Induksi Hiperglikemia

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Sri Lestari Dwi Astuti ◽  
Yeni Tutu Rohimah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Olive Oil ◽  
Glucose Level ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Standard Diet

Background: The global population of diabetes mellitus tends to increase every year. This is related to the increased of population numbers, lifestyle, increased prevalence of obesity and less physical activity (Smeltzer & Bare, 2002). Olive oil is a functional diet because it is rich of monounsaturated fatty acids (MUFA), especially oleic acid (70% - 80%), contains antioxidants and polyphenol compounds. Polyphenols are α-amylase enzyme inhibitors that have function in the breakdown of carbohydrates, so that the level of blood glucose in hyperglycemia can be lowered (Astrawan made, Wresdiyati Tutik, Nasution Nurlayla Amas, 2015). The goal of this study is to examine the effectiveness of extra virgin olive oil to blood glucose level. Method:This study used experimental laboratory study with post test only control group design. The independent variable was the extra virgin olive oil, while the dependent variable was the blood glucose level. The samples were 32 healthy 8 weeks old white male wistar strain rats with the weigh between 180 mg - 200 mg. It divided into 3 groups, the negative control group was fed with standard diet, the positive control group was fed with standard diet and 1 ml fructose for 4 weeks, the treatment group was fed with standard diet, 1 ml fructose and 0.5 gr extra virgin olive oil for 4 weeks, on the fifth week the fructose was increased into 4 ml/day for the next 4 weeks, on the 52nd day the blood glucose level was checked after it was fasted for 6 hours. The data analysis used t-test and Mann Withney test with α = 0.05. Result: The blood glucose level average of the positive control group was 309.57 ± 120.12, the blood glucose level average of the negative control group was 111.85 ± 10.57, the blood glucose level average of the treatment group was 115.12 ± 12.25. Conclusion: Giving the extra virgin olive oil can effectively lower the blood glucose level on the male wistar rats.

Metrological support of individual blood glucose meters in telemedicine

2019 ◽  
pp. 52-56
Author(s):  
Yu.F. Glukhov ◽  
N.V. Krutikov ◽  
A.V. Ivanov ◽  
N.P. Muravskaya
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Measurement Data ◽  
Test Method ◽  
Level Measurement ◽  
Measurement Devices ◽  
Real Practice ◽  
Health Care Centers ◽  
Medical Measurement

We have studied and analyzed status and metrological supervision of blood glucose monitors, individual devices for a person’s blood glucose level measurement. It has been indicated that nowadays blood glucose monitors like other individual devices for medical measurement are not allowed to be involved in telemedicine public service. This accounts for absence of metrological supervision with these measurement devices in telemedicine. In addition, the key problem is absence of safe methods and means of remote verificaition, calibration and transmission of measurement data to health care centers. The article offers a remote test method for blood glucose monitors using a number of resistors with values correlating with measured blood glucose level. The available method has been successfully trialed in real practice.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

scholarly journals EFFECTIVENESS OF AMLA JUICE IN REDUCING BLOOD GLUCOSE LEVEL AMONG PATIENTS WITH TYPE II DIABETES IN A SELECTED AREA OF KANYAKUMARI DISTRICT

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mrs. Vanitha. S s ◽  
Dr. Pramjit kaur
Keyword(s):  
Blood Glucose ◽  
Standard Deviation ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Type Ii Diabetes ◽  
Control Group ◽  
Type Ii ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Experimental Group

Challenges in lifestyle, such as increasesin energy intake and decreasesin physical activity are causing overweight and obesity leading to epidemic increases in type II Diabetes Mellitus. The research approach used for this study was evaluative approach and the research design was true experimental design. 60 patients with type II diabetes, 30 in experimental group and 30 in control group were selected for this study by using purposive sampling technique. Data was collected with the help of self-structured interview schedule. Descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (chi-square, paired ‘t’ test) were used to analyse the data and to test the hypotheses. In the experimental group,the pre-test mean score was 2.966, mean percentage was 59% and standard deviation was 1.129 and in post-testmean score was 2.533, mean percentage was 50.66% and standard deviation was 1.074 with effectiveness of 8.34% and paired‘t’ test value of t=3.971,which was statistically significant (p<0.05) which is an evidence ofthe effectiveness of Amla juice in reducing blood glucose level. Comparison of blood glucose levels in experimental and control groups, shows that the value is statistically highly significant, as was observed from the unpaired ‘t’ test value of 13.39 with P value of <0.05, which is an evidence indicatingthe effect of Amla juice in reducing postprandial blood glucose levels. The resultsfound that the administration of Amla juice did have aneffect in reducing blood glucose level in the experimental group. By comparing the findings of pre-test and post test between the experimental group and the control group,the effect was identified (assessed). The study concluded that the Amlajuice is effective in reducing blood glucose level.

scholarly journals ANTI-HYPERGLYCEMIC EFFECT OF DIFFERENT FRACTIONS OF ANNONA RETICULATA LEAF

2016 ◽  
pp. 256
Author(s):  
Soumya Prakash Rout ◽  
Durga Madhab Kar ◽  
Laxmidhar Maharana
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Ethyl Acetate Fraction ◽  
Diabetic Control ◽  
Control Group ◽  
Alcoholic Extract ◽  
Diabetic Control Group ◽  
Annona Reticulata

<p>ABSTRACT<br />Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae)<br />an interesting plant for investigating its anti-hyperglycemic potential.<br />Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on<br />Streptozotocin (STZ) induced hyperglycemic rats.<br />Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of<br />A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential.<br />Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level.<br />Results: The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is<br />significant (p&lt;0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from<br />417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug<br />metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL.<br />Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate<br />certain secondary parameters associated with STZ induced hyperglycemia.<br />Conclusion: This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic<br />property<br />in the tested<br />experimental<br />models and should be investigated<br />further.<br />Keywords: Streptozotocin, Diabetes, Dyslipidemia.</p>

scholarly journals Pengaruh suplementasi β-carotene terhadap kadar glukosa darah dan kadar malondialdehida pada tikus sprague dawley yang diinduksi Streptozotocin

2014 ◽  
Vol 2 (2) ◽  
pp. 41-46
Author(s):  
Elida Soviana ◽  
Banundari Rachmawati ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Negative Control ◽  
Male Rats ◽  
Sprague Dawley ◽  
Significant Difference ◽  
Decrease Blood Glucose ◽  
Β Carotene

Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). β-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses β-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). β-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation β-carotene on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses β-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. β-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and β-carotene 20 mg/kg BW to decrease MDA level.

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