PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculata) TERHADAP KADAR GLUKOSA DARAH MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN (Effect of Long Bean (Vigna unguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan)

Intan Fitri Aprila; M. Nur Salim; Razali Daud; T. Armansyah; Nuzul Asmilia; Faisal Jamin

doi:10.21157/j.med.vet..v9i2.3983

Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3490 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

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Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

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Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

Nusantara Bioscience ◽

10.13057/nusbiosci/n090312 ◽

2017 ◽

Vol 9 (3) ◽

pp. 312-317

Author(s):

KARTIAWATI ALIPIN ◽

EMA PURNAMA SARI ◽

MADIHAH MADIHAH ◽

TIA SETIAWATI ◽

NINING RATNINGSIH ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Positive Control ◽

Negative Control ◽

Kidney Damage ◽

Diabetic Rat ◽

Control Group ◽

Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level â‰¥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

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Efektifitas Pemberian Minyak Zaitun Ekstra Virgin Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Dengan Induksi Hiperglikemia

Interest : Jurnal Ilmu Kesehatan ◽

10.37341/interest.v7i2.25 ◽

2018 ◽

Vol 7 (2) ◽

Author(s):

Sri Lestari Dwi Astuti ◽

Yeni Tutu Rohimah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Olive Oil ◽

Glucose Level ◽

Virgin Olive Oil ◽

Extra Virgin Olive Oil ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Standard Diet

Background: The global population of diabetes mellitus tends to increase every year. This is related to the increased of population numbers, lifestyle, increased prevalence of obesity and less physical activity (Smeltzer & Bare, 2002). Olive oil is a functional diet because it is rich of monounsaturated fatty acids (MUFA), especially oleic acid (70% - 80%), contains antioxidants and polyphenol compounds. Polyphenols are α-amylase enzyme inhibitors that have function in the breakdown of carbohydrates, so that the level of blood glucose in hyperglycemia can be lowered (Astrawan made, Wresdiyati Tutik, Nasution Nurlayla Amas, 2015). The goal of this study is to examine the effectiveness of extra virgin olive oil to blood glucose level. Method:This study used experimental laboratory study with post test only control group design. The independent variable was the extra virgin olive oil, while the dependent variable was the blood glucose level. The samples were 32 healthy 8 weeks old white male wistar strain rats with the weigh between 180 mg - 200 mg. It divided into 3 groups, the negative control group was fed with standard diet, the positive control group was fed with standard diet and 1 ml fructose for 4 weeks, the treatment group was fed with standard diet, 1 ml fructose and 0.5 gr extra virgin olive oil for 4 weeks, on the fifth week the fructose was increased into 4 ml/day for the next 4 weeks, on the 52nd day the blood glucose level was checked after it was fasted for 6 hours. The data analysis used t-test and Mann Withney test with α = 0.05. Result: The blood glucose level average of the positive control group was 309.57 ± 120.12, the blood glucose level average of the negative control group was 111.85 ± 10.57, the blood glucose level average of the treatment group was 115.12 ± 12.25. Conclusion: Giving the extra virgin olive oil can effectively lower the blood glucose level on the male wistar rats.

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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UJI PERBANDINGAN EFEKTIVITAS ANTIDIABETES EKSTRAK POLISAKARIDA DAN SENYAWA POLIFENOL ALGA COKLAT (Sargassum sp.) PADA MENCIT YANG DIINDUKSI ALOKSAN

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v4i1.157 ◽

2018 ◽

Vol 4 (1) ◽

pp. 48

Author(s):

Agung Giri Samudra ◽

Fathur Sani K ◽

Moniq Chintama

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Brown Algae ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Group Iii ◽

Percentage Decrease ◽

Polyphenolic Extract

Diabetes is one of the most common diseases in the world. So there is an effort to prevent or treat the disease. As brown algae (Sargassum sp.) Contains polysaccharides that regulate sugar intake in the body, and polyphenols act as antioxidants that can stimulate insulin secretion in pancreatic β cells. This study aims to determine the effectiveness of Antidiabetes Polysaccharide Extract and Brown Algae Polifenol Compounds (Sargassum sp) On Alloxaninduced Mice. The test animals were grouped into 4 groups I (CMC 1%), Group II (Glibenclamide 0.52mg / 20grBB), Group III (5mg / 20grBB polyphenol extract), Group IV (5mg / 20grBB polysaccharide extract). Blood glucose was measured using Easy touch glucometer on days 0, 3, 7, 14, 21, and 28. Analyze data using one-way ANOVA, followed by LSD test. The percentage decrease in blood glucose level in positive control, polyphenol extract and polysaccharide were 42,86%, 58,12%, and 54,20%. Statistically, the polyphenolic extract and the brown algae polysaccharide lower the blood glucose level significantly compared with the negative control (p≤0,05). Decreased blood glucose in polyphenol extracts and polysaccharides showed statistically significant differences

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Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6628 ◽

2021 ◽

Vol 9 (A) ◽

pp. 760-765

Author(s):

Yos Banne ◽

Olfie Sahelangi ◽

Steven Soenjono ◽

Elisabeth Natalia Barung ◽

Selfie Ulaen ◽

...

Keyword(s):

Silver Nanoparticles ◽

Silver Nanoparticle ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Probit Analysis ◽

Control Group ◽

Distilled Water ◽

Lc50 Value ◽

Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

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Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v8i02.34284 ◽

2020 ◽

Vol 8 (02) ◽

pp. 35-39

Author(s):

Laxmi Shrestha ◽

Amit Shrivastava ◽

Bishal Joshi ◽

Buddhi Raj Pokhrel ◽

Shanti Gurung ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Aqueous Extract ◽

Nigella Sativa ◽

Negative Control ◽

Hypoglycemic Effect ◽

Control Group ◽

Oral Glucose ◽

Standard Group

INTRODUCTION Diabetes mellitus is the most common metabolic disease worldwide. Nigella sativa also known as black cumin, is an emerging miracle herb with a rich historical and religious background. In the present study, we have attempted to study the hypoglycemic effect of aqueous extract of Nigella sativa on diabetes-induced rats. MATERIAL AND METHODS This preclinical study was conducted in Department of Pharmacology from August to October 2019. In this study, hyperglycemia was induced by a single intraperitoneal dose of 60 mg/kg streptozotocin in 0.1 M citrate buffer (pH 4.5). Animals were divided into five groups containing normal control, negative control, standard, test-1, and test-2. The aqueous extract of Nigella sativa was administered 400 and 800 mg/kg per oral in both the test groups for 18 days. Blood glucose level was measured at three-days interval by glucometer. Oral glucose tolerance test was done by administrating 2 gm/kg per oral glucose and the blood glucose was recorded every two-hours interval for eight hours. RESULTS An oral glucose tolerance test showed a decrease in blood glucose level at the dose of 400 and 800 mg/kg compared to the negative control group. The administration of aqueous extract of Nigella sativa for 18 days showed significant decrease in blood glucose level (p=0.001) compared to negative control group. The blood glucose level in test 1 (400 mg/kg) was significantly reduced compared to the standard group (p***<0.001). The blood glucose level in test 2 at 18th day was less significant (p*< 0.05) compared with standard group and test 1 (p**<0.01, p***<0.001). CONCLUSION The findings of the present study indicate that the aqueous extract of Nigella sativa has significant hypoglycemic effect in Wistar rats.

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Potensi Minyak Sawit Merah Dan Karotenoid Sebagai Suplemen Antioksidan Dalam Pengujian Toleransi Glukosa Pada Tikus Putih (Preliminary Study)

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i1.84 ◽

2018 ◽

Vol 1 (1) ◽

pp. 251-256 ◽

Cited By ~ 1

Author(s):

Ahmad Gazali Sofwan Sinaga ◽

Donald Siahaan ◽

Kasmirul Ramlan Sinaga

Keyword(s):

Blood Glucose ◽

Palm Oil ◽

Preliminary Test ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Red Palm Oil ◽

Dpph Method ◽

Anti Oxidant

Minyak sawit merah (MSM) adalah salah satu produk olahan dari minyak kelapa sawit, dan merupakan sumber makanan yang kaya karotenoid sekitar 500 - 800 ppm. Konsentrat karotenoid (KK) juga merupakan produk yang diperoleh dari proses penghilangan minyak sehingga kadar karotenoid meningkat hingga 6000 - 7000 ppm. Penelitian ini bertujuan untuk mengetahui potensi penggunaan minyak sawit merah dan konsentrat karotenoid sebagai uji pendahuluan suplemen antioksidan dalam pengujian toleransi glukosa pada tikus putih. Pengujian aktivitas antioksidan dari minyak sawit merah dan konsentrat karotenoid diuji dengan metode DPPH dengan masa inkubasi selama 60 menit. Pengujian efektivitas antihiperglikemia dilakukan menggunakan hewan percobaan (n=40) yang dibagi menjadi 8 grup: grup kontrol negatif (akuades), kontrol positif (glibenklamid 10 mg), tiga grup diberi minyak sawit merah dengan dosis 1, 2, dan 3 ml, dan tiga grup diberi konsentrat karotenoid dengan dosis 1, 2, dan 3 ml. Penurunan kadar glukosa darah selama 180 menit pada uji antihiperglikemia sebagai berikut glibenklamid 10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml dan akuades. Pemberian minyak sawit merah dapat menurunkan kadar glukosa darah lebih cepat dibandingkan konsentrat karotenoid. Sifat antioksidatif komponen minor minyak sawit merah dapat menghambat oksidasi glukosa di dalam darah, sehingga pankreas dapat aktif memproduksi insulin secara normal. Oleh karena itu, minyak sawit merah dapat digunakan secara sinergis untuk membantu kerja obat hiperglikemia oral (OHO). Red Palm Oil is one of processed products from palm oil and also a food source rich in carotenoid,around500-800 ppm. Carotenoid concentrate is also a product obtained from the oil removal process and the carotenoid level increases to 6000-7000 ppm. This research aimed to determinethe potential of the red palm oil and the carotenoid concentrate as a preliminary test of anti-oxidant supplement Glucose Tolerance Test on Rat. The anti-oxidant activity of red palm oil and carotenoid concentrate was tested using DPPH method with 60 minutes incubation period. The anti-hyperglycemia activity evaluationwas conducted using animals(n=40) which divided into 8 groups: Negative control group (distilled water), positive control (glibenclamide 10 mg), three groups were given red palm oil with dose of1, 2, and 3 ml , and three groups were given carotenoid concentrate with dose of 1, 2, and 3 ml . The decrease of blood glucose for 180 minutes in anti-hyperglycemia test were in order ofglibenclamide10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml and distilled water. The red palm oil induction could lower blood glucose level faster than carotenoid concentrate, The anti-oxidative properties of minor componentsin red palm oil prevent the blood glucose oxidation and the pancreas could be actively produce insulin normally. In conclusion, red palm oil could be used synergistically toenhance the effectiveness oforal hyperglycemic agent.

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Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell)

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v13i1.384 ◽

2014 ◽

Vol 13 (1) ◽

pp. 33

Author(s):

Maharani Laillyza Apriasari ◽

Amy Nindia Carabelly ◽

Gusti Febby Aprilia

Keyword(s):

Mus Musculus ◽

Healing Process ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Banana Plant ◽

Significant Difference ◽

Stem Extract ◽

Banana Stem

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.

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