scholarly journals ASSOCIATION OF IL5, CCL26 AND CCL5 GENE POLYMORPHISMSWITH ATOPIC BRONCHIAL ASTHMA

2010 ◽  
Vol 7 (6) ◽  
pp. 20-24
Author(s):  
O E Voronko ◽  
E V Dmitrieva-Zdorova ◽  
E A Latysheva ◽  
M G Aksenova ◽  
G I Storozhakov ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Single Nucleotide Polymorphisms ◽  
Bronchial Asthma ◽  
Asthma Severity ◽  
Moscow Region ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Atopic Bronchial Asthma ◽  
Healthy Donors

The purpose of the work. In order to investigate whether single nucleotide polymorphisms С(-703)Т in IL5 gene, T(+2497)G in CCL26 and A(-403)G in CCL5 gene contribute to atopic bronchial asthma development we performed a comparative analysis of alleles and genotypes frequencies of these polymorphisms in Russian patients from Moscow region. Materials and methods. DNA samples from 283 patients with atopic bronchial asthma and 227 healthy donors were genotyped. Results. There were associations neither of С(-703)Т in IL5 gene, T(+2497)G in CCL26 and A(-403)G in CCL5 gene with asthma nor with asthma severity. Conclusion. Therefore, we may conclude that genes responsible for activation and chemotaxis of eosinophils play an insignificant role in susceptibility to development of atopic bronchial asthma, and probably may influence indirectly specific processes that occur in asthma.-

Nuclear factor erythroid 2-related factor gene variants and susceptibility of arsenic-related skin lesions

2013 ◽  
Vol 33 (6) ◽  
pp. 582-589 ◽  
Author(s):  
EJ Cordova ◽  
OL Valenzuela ◽  
LC Sánchez-Peña ◽  
G Escamilla-Guerrero ◽  
A Hernández-Zavala ◽  
...  
Keyword(s):  
Skin Lesion ◽  
Single Nucleotide Polymorphisms ◽  
Regulatory Region ◽  
Inorganic Arsenic ◽  
Skin Lesions ◽  
Related Factor ◽  
Nuclear Factor ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Healthy Donors

Inorganic arsenic (iAs) is an important pollutant associated with various chronic-degenerative diseases. The cytoprotective protein nuclear factor erythroid 2-related factor (NRF2) has been proposed as an important responsive mechanism against iAs exposure. The aim of this study was to determine whether the risk of skin lesions in people exposed to iAs-contaminated water could be modified by the presence of single nucleotide polymorphisms in the NRF2 coding gene. We studied 117 individuals with long-term iAs exposure and 120 nonexposed individuals. Total As was determined in water, meanwhile iAs and its metabolites were measured in urine. The iAs-induced skin lesion status was evaluated by expert dermatologists. We sequenced the promoter region of NRF2 in a sample of 120 healthy donors. We found four polymorphisms previously reported and one novel polymorphism in the 5′ regulatory region of the NRF2. In this study, we did not find allelic and genotype association of NRF2 polymorphisms with iAs-related skin lesion. However, the analysis of haplotypes composed by –653GA, and –617CA NRF2 single nucleotide polymorphisms showed a significant association with protection against skin lesions in the low-As exposure group. This is the first report studying the association between NRF2 polymorphisms and susceptibility of As-related skin lesions. Increasing the sample size will allow us to confirm this data.

scholarly journals Comparative analysis of microsatellite and SNP markers for parentage testing in the golden snub-nosed monkey (Rhinopithecus roxellana)

2020 ◽  
Vol 12 (4) ◽  
pp. 611-620
Author(s):  
Chen Ling ◽  
Wu Lixia ◽  
Hou Rong ◽  
Shen Fujun ◽  
Zhang Wenping ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Single Nucleotide Polymorphisms ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Snp Markers ◽  
Rhinopithecus Roxellana ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Parentage Testing ◽  
International Human

Abstract Microsatellite markers are popular for assigning parentage, but single-nucleotide polymorphisms (SNPs) have only been applied in this area recently. To evaluate these two markers which have been previously studied in golden snub-nosed monkeys, we genotyped 12 individuals using 37 microsatellite loci and 37 SNP markers. The data showed that 32 of 37 microsatellite loci were polymorphic, and most microsatellite loci were high informative (mean PIC = 0.599). Meanwhile, 24 of 37 SNP markers were polymorphic and most were low informative (mean PIC = 0.244). For microsatellites, the combined exclusion probability with one-parent-unknown/known (CE-1P/CE-2P) nearly reached 1, while for the SNP markers, CE-2P only reached 0.9582. Under the condition of one parent known/unknown, the CE-2P and CE-1P could meet the international human parental standard (0.9973) by using five or nine microsatellite loci respectively. For SNP markers, we doubled the loci (n = 48) and simulated parentage testing, and the data showed that the CE-2P was 0.998 while the CE-1P was still low. This result indicated that the SNP loci which we used here had low polymorphism and that more loci need to be developed in the future. In addition, we corrected one case of failed identification by excluding siblings and reducing the range of candidate paternities.

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