scholarly journals BIO-FLOCCULATION AND ANTIMICROBIAL ACTIVITIES OF POWDERED MORINGA OLEIFERA (LAM) SEEDS AND ALUM ON DOMESTIC WASTEWATER MICROBIAL CONSORTIA

2021 ◽  
pp. e267
Author(s):  
Olubukola Olusola-makinde ◽  
Michael Bayode ◽  
Eniola Dawodu
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Moringa Oleifera ◽  
Domestic Wastewater ◽  
Antimicrobial Activities ◽  
Microbial Consortia ◽  
16S Rna ◽  
Enterobacter Ludwigii ◽  
Wastewater Samples

Introduction: Moringa oleifera seed is a bio-flocculant liable to purify water and verified to be one of the generally efficient prime coagulants for water treatment. M. oleifera seeds also have the potentials to eliminate a broad variety of bacteria, including Escherichia coli, Bacillus subtilis, B. cereus, Pseudomonas aeruginosa and Enterobacter ludwigii, from domestic wastewater. Objective: The comparative bio-flocculating ability and antimicrobial activities of powdered Moringa oleifera seeds and alum for the treatment of domestic wastewater from a university student’ hostels were explored. Methods: Collection of wastewater samples, physicochemical analysis of wastewater samples and treatment of the wastewater samples with powdered M. oleifera seeds and alum were conducted using standard techniques. Enumeration and identification of bacteria using biochemical depiction and (16S RNA) with fungi after treatment were employed via standard protocols. Results: The optimum pH obtained using powdered M. oleifera seeds was 6.00 – 7.38 and in line with the recommended WHO standard. This study revealed that the bacterial count in wastewater samples of Jibowu and Abiola hostels after treatment with 2g of powdered M. oleifera seeds and 6g of alum was high (199.67±0.89 CFU/ml); low (26.00±0.57 CFU/ml) for powdered M. oleifera seeds and high (87.00±0.57 CFU/ml); low (6.33±0.57 CFU/ml) for alum respectively. The fungal count of the wastewater samples for Akindeko and Jibowu hostels after treatment with 2g of powdered M. oleifera seeds and 6g of alum was high (26.00±0.57 Sfu/ml); low (5.00±0.57 Sfu/ml) for powdered M. oleifera seeds and high (19.00±0.58 Sfu/ml); low (2.00±0.57 Sfu/ml) for alum respectively. Escherichia coli, Bacillus subtilis with NCBI-certified B. cereus mkbk1, Pseudomonas aeruginosa mkbk 2 and Enterobacter ludwigii mkbk 3 were isolated from the wastewater samples. Conclusion: The findings of this study suggest that the bio-flocculating ability of powdered M. oleifera seeds accentuated better antimicrobial efficacy of M. oleifera over alum as a proviso to the blend of powdered M. oleifera seeds and alum for the treatment of domestic wastewaters.

scholarly journals Evaluation of antimicrobial potential and phytochemical screening of Moringa oleifera leave extract: An Emerging folkloric medicinal plant in Maiduguri, Borno state, Nigeria

2020 ◽  
Vol 4 (3) ◽  
pp. 043-047
Author(s):  
Fatima Adamu Lawan ◽  
Binta Baba Shehu ◽  
Ali Abba Gana Benisheikh ◽  
Abba Mohammad Dige ◽  
Hassan Arabi Jidda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Medicinal Plant ◽  
Moringa Oleifera ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Antimicrobial Potential ◽  
Agar Disc

The aim of this study was to evaluate the antimicrobial potential and phytochemical screening of Moringa oleifera leave extract as folkloric medicinal alternative. The phytochemical screening result revealed the presence of Alkanoids, Saponins, Fats & oil, Terpenoids and Tanin in all the solvents used for the study. Agar-disc diffusion method were used to determine the antimicrobial activities of the Moringa oleifera extracts on microorganisms. Maximum activities of inhibition were recorded on Ethyl acetate (50mg/ml) against Pseudomonas auroginusa, moderarate activities against Escherichia coli. Likewise 50mg/ml of Haxane extract show weak activities on Pseudomonas aeruginosa, moderate in both Staphalococcus aureus and Escherichia coli. Whereas 50mg/ml of Methanol show moderate activity on Pseudomonas aeruginosa, Staphalococcus aureus and weak activities on Escherichia coli respectively. The research revealed that Moringa oleifera bioactive components would be a safer alternative for curative and preventive emerging folkloric medicinal plant for treatment of bacterial ailments in Maiduguri, Borno state, Nigeria.

scholarly journals Synthesis and Antimicrobial Activities of 1-((5-Chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide

2021 ◽  
Vol 47 (3) ◽  
pp. 1296-1302
Author(s):  
Oluwaseyi B. Ovonramwen ◽  
Bodunde J. Owolabi ◽  
Abiodun Falodun
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Activities ◽  
Microbiological Method ◽  
Gram Negative ◽  
Methyl Imidazole

A new 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide was synthesized from methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate and ethylamine. The compound methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate was synthesized from methyl pyrrolidine-2-carboxylate and 5-chloro-4-chlorosulfonyl-1-ethyl-2-methyl-imidazole. The compounds were characterized based on FTIR, 1H, 13C NMR, and DEPT 135 analysis. Antimicrobial activities of the 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide against Gram-positive (methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Bacillus subtilis), Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae), and Candida albicans were carried out using the standard microbiological method. The newly synthesized 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide had no activities against the tested organisms. Keywords:    1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide; methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate; L-proline; ethylamine.

Determination of Usnic Acid in Some Rhizoplaca Species from Middle Anatolia and their Antimicrobial Activities

2006 ◽  
Vol 61 (1-2) ◽  
pp. 47-51 ◽  
Author(s):  
Demet Cansaran ◽  
Demet Çetin ◽  
M. Gökhan Halıcı ◽  
Orhan Atakol
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Enterococcus Faecalis ◽  
Usnic Acid ◽  
Dry Weight ◽  
Antimicrobial Activities ◽  
Acetone Extracts

Abstract Three species of lichens, Rhizoplaca chrysoleuca (Sm.) Zopf, Rhizoplaca melanopthalma (DC.) Leuckert & Poelt and Rhizoplaca peltata Ramonds Leuckert, were collected from middle Anatolia, Erciyes Mountain (Kayseri) in Turkey. Their usnic acid amounts were determined by HPLC in acetone extracts. In addition, antimicrobial activities of these extracts were determined against Escherichia coli (ATCC 35218), Enterococcus faecalis (RSKK 508), Proteus mirabilis (Pasteur Ens. 235), Staphylococcus aureus, Bacillus subtilis, Bacillus megaterium and Pseudomonas aeruginosa. It was shown that, as the usnic acid amount increased, the antimicrobial activities increased too. The usnic acid contents of Rhizoplaca species varied between 0.19-4.0% dry weight

scholarly journals Comparative evaluation of hydrophilic bases for improved delivery of Benzoic acid and Salicylic acid in antimicrobial ointment

2021 ◽  
Vol 22 (1) ◽  
pp. 74-87
Author(s):  
A.O. Oyedele ◽  
E.O. Akinkunmi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salicylic Acid ◽  
Benzoic Acid ◽  
Antimicrobial Activities ◽  
Activité Antimicrobienne ◽  
Candida Pseudotropicalis

Background: Benzoic acid (BA) and salicylic acid (SA) combined are used as choice topical treatment for fungal and bacterial infections but their delivery is affected by ointment vehicle, among other factors. With aim to achieve improved release and antimicrobial activity in ointment formulation of these medicaments through selection of more efficient vehicle(s), this study comparatively evaluated prospective alternatives to thecompendium-specified base for delivery potential and relevant physical properties.Methodology: Water-sorption capacity, rheological characteristics and heat-tolerance limits of six hydrophilic bases [hydrous ointment (HO), hydrous sheabutter (HS), neat sheabutter (NS), lanolin anhydrous (LA), lanolin hydrous (LH), and emulsifying ointment (EO)] and their ointment products containing 1, 2, 3, or 6% w/w of BA or SA were determined. Drug delivery propensity of the products was evaluated by agar diffusion colorimetric assay, while their antimicrobial activities were determined by hole-in-plate agar diffusion assay against selected type organisms (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida pseudotropicalis).Results: BA was released in relatively larger quantity and demonstrated greater antimicrobial activity than SA in most bases. The released quantities of each medicament correlated directly with concentration and antimicrobial activities. The rates and extents of drug release followed a similar trend in different vehicles namely, HO > HS > NS ≥ EO > LA ≈ LH. Water number of base, ointment preparation method, viscosity, or heat-tolerance showed no influence on drug release or antimicrobial activity.Conclusion: HO and HS are better vehicles for delivery of BA and SA in ointment than EO which is the prototypical base. Keywords: Benzoic acid, Salicylic acid, Antimicrobial activity, Ointment base, Delivery factors French Title: Évaluation comparative des bases hydrophiles pour une meilleure administration d'acide benzoïque et d'acide salicylique dans une pommade antimicrobienne Contexte: L'acide benzoïque (BA) et l'acide salicylique (SA) combinés sont utilisés comme traitement topique de choix pour les infections fongiques et bactériennes, mais leur administration est affectée par le véhicule de la pommade, entre autres facteurs. Dans le but d'améliorer la libération et l'activité antimicrobienne dans la  formulation de pommade de ces médicaments grâce à la sélection de véhicules plus efficaces, cette étude a évalué comparativement des alternatives potentielles à la base spécifiée dans le compendium pour le potentiel d'administration et les propriétés physiques pertinentes.Méthodologie: Capacité de sorption de l'eau, caractéristiques rhéologiques et limites de tolérance à la chaleur de six bases hydrophiles [pommade hydratée (HO), beurre de karité hydraté (HS), beurre de karité pur (NS), lanoline anhydre (LA), lanoline hydratée (LH), et onguent émulsifiant (EO)] et leurs produits de pommade contenant 1, 2, 3 ou 6% p/p de BA ou SA ont été déterminés. La propension à l'administration de médicaments des produits a été évaluée par un essai colorimétrique de diffusion sur gélose, tandis que leurs activités antimicrobiennes ont été déterminées par un essai de diffusion sur gélose en plaque contre des organismes de type sélectionné (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans et Candida pseudotropicalis).Résultats: BA a été libéré en quantité relativement plus grande et a démontré une plus grande activité antimicrobienne que SA dans la plupart des bases. Les quantités libérées de chaque médicament étaient directement liées à la concentration et aux activités antimicrobiennes. Les taux et l'étendue de la libération de médicaments ont suivi une tendance similaire dans différents véhicules, à savoir HO> HS> NS ≥ EO> LA ≈ LH. Le nombre d'eau de base, la méthode de préparation de la pommade, la viscosité ou la tolérance à la chaleur n'ont montré aucune influence sur la libération du médicament ou l'activité antimicrobienne.Conclusion: HO et HS sont de meilleurs véhicules pour la livraison de BA et SA en onguent que EO qui est la base prototypique. Mots-clés: acide benzoïque, acide salicylique, activité antimicrobienne, base de pommade, facteurs de délivrance  

scholarly journals Antimicrobial Activities of Novel Bis-Piperidinium Compounds

2012 ◽  
Vol 61 (3) ◽  
pp. 223-225
Author(s):  
MUHAMMAD JAWWAD SAIF ◽  
MOHAMMED ZUBER ◽  
JAMIL ANWAR ◽  
MUNAWAR ALI MUNAWAR
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Alkyl Chains ◽  
Dodecyltrimethylammonium Chloride

The antimicrobial activity of two new series of bis-piperidinium compounds with alkyl chains of different lengths against bacterial (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis) and fungal strains (Aspergillus flavus, Aspergillus niger, Rhodolorula rubera, Lipomyces lopofera and Candida albicans), are described. Antimicrobial activities of the synthesized compounds were compared to that of dodecyltrimethylammonium chloride. Bis-piperidinium salts possessing 12-16 carbon side chains showed better antimicrobial properties as compared to the standard dodecyltrimethylammonium chloride.

XÁC ĐỊNH THÀNH PHẦN HÓA HỌC VÀ KHẢ NĂNG KHÁNG VI SINH VẬT CỦA CAO CHIẾT ETHANOL LY TRÍCH TỪ THÂN RỄ LOÀI THIÊN NIÊN KIỆN PI-E (HOMALOMENA PIERREANA)

2020 ◽  
Vol 39 (03) ◽  
Author(s):  
VĂN HỒNG THIỆN ◽  
LÊ BÍCH TRÂM ◽  
NGUYỄN THANH LAN ◽  
HỒ NGUYỄN HOÀNG YẾN ◽  
LƯU HỒNG TRƯỜNG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis

Nghiên cứu này đã xác định được mẫu nghiên cứu thu tại Vườn quốc gia Phú Quốc là loài Homalomena pierreana. Thông qua kỹ thuật sắc ký ghép khối phổ, 10 hợp chất thuộc nhóm sesquiterpene có trong cao chiết ethanol của thân rễ loài H. pierreana đã được xác định. Ngoài ra, cao chiết ethanol từ mẫu nghiên cứu cũng cho thấy khả năng kháng lại 6 chủng vi khuẩn kiểm định là Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis và Salmonella typhimurium.

scholarly journals ANTIMICROBIAL ACTIVITY OF SELECTED INDIAN FOLK MEDICINAL PLANTS: MYRISTICA FATUA, ALSTONIA BOONEI, HELICTERES ISORA, VITEX ALTISSIMA AND ATALANTIA RACEMOSA

2017 ◽  
Vol 10 (2) ◽  
pp. 277
Author(s):  
Kokkaiah Irulandi ◽  
Sethupandian Geetha ◽  
Palanichamy Mehalingam
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Alternative Source ◽  
Helicteres Isora ◽  
Acetone Extracts

Objective: To determine antimicrobial activity of methanol, ethyl acetate and acetone extracts of Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa  against different species of pathogens, Streptococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphyllococcus aureus, and Candida albicans.Methods: Antimicrobial activity of plant extracts was measured by agar well diffusion method.Results: Acetone extracts of Alstonia boonei showed the highest inhibitory effect against Escherichia coli (21.00 ± 1.00 mm) and Streptococcus faecalis (19.00 ± 1.00 mm). All the extracts of Heliteres isora leaves showed different zone of inhibition observed in all the tested pathogens ranges between (8.13 ± 1.53 – 15.25 ± 1.23 mm). Ethyl acetate extract of Vitex altissima showed highest activity against Bacillus sublilis (19.67 ± 1.53 mm). Methanol and acetone leaves extracts of Atalantia racemosa have good fungal activity against the Candida albicans (19.33 ± 1.26 mm - 16.00 ± 1.00 mm). Methanol extract of Myristica fatua showed high antimicrobial activity against Pseudomonas aeruginosa (15.10 ± 0.17 mm) and Bacillus subtilis (14.23 ± 0.21 mm).Conclusion: The results from the study suggest that the leaves Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa showed good antimicrobial activity against the different pathogens. They are used as the alternative source for the control and treatment of microbial infections.Keywords: Antimicrobial activity, Leaves extracts,  Well diffusion method, Pathogenic strains.

scholarly journals ANÁLISE DA COR E DA ATIVIDADE ANTIMICROBIANA DE MICROCÁPSULAS CONTENDO LACTOFERRINA BOVINA

2014 ◽  
Vol 32 (2) ◽  
Author(s):  
LUCIANA HELENA MAIA PORTE ◽  
MARIA HELENA MIGUEZ ROCHA LEÃO ◽  
ALEXANDRE PORTE
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Spray Drying ◽  
Enterococcus Faecium ◽  
Micrococcus Luteus ◽  
Rhodococcus Equi ◽  
Streptococcus Faecium

Lactoferrina bovina (bLF), proveniente do soro de leite, foimicroencapsulada pela técnica de spray drying. Microcápsulascontendo 20 % de bLF foram produzidas, utilizando-se comomaterial de parede dextrina: amido octenilsuccinato (OSA) emdiferentes proporções: 100:00, 75:25, 50:50, 25:75 e 0:100 %.Foram avaliadas a cor e a estabilidade de cor das microcápsulassob armazenamento em ambientes com diferentes umidadesrelativas e a atividade antimicrobiana da lactoferrina liberadadas microcápsulas. As microcápsulas apresentaram cor clara etenderam a escurecer sob armazenamento em ambiente com altaumidade relativa. Verifi cou-se atividade inibitória das microcápsulasde bLF produzidas para diferentes bactérias Gram positivas(Bacillus subtilis CCT 2576, Staphylococcus aureus CCT 2740,Micrococcus luteus CCT 2692, Enterococcus faecium CCT 5079,Streptococcus faecium ATCC 10541, Rhodococcus equi CCT0541), Gram negativas (Pseudomonas aeruginosa ATCC 13388,Salmonella choleraesius CCT 4296, Escherichia coli CCT 0547) elevedura (Candida albicans ATCC 10231). A concentração inibitóriamínima (MIC) das microcápsulas variou de acordo com o microorganismotestado (MIC entre 2,5-100 mg.mL-1). Com exceção deB. subtilis (MIC entre 50-100 mg.mL-1 para as microcápsulas), aconcentração de bLF contida nas microcápsulas necessária parainibir o crescimento dos micro-organismos foi menor do que a bLFnativa. Esses resultados sugerem efeito de potencialização daatividade antimicrobiana da bLF após o processamento por spraydrying.

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

scholarly journals Green synthesis of Ag, Zn and Cu nanoparticles from aqueous extract of Spondias mombin leaves and evaluation of their antibacterial activity

2020 ◽  
Vol 21 (2) ◽  
pp. 106-113
Author(s):  
D.K. Adeyemi ◽  
A.O. Adeluola ◽  
M.J. Akinbile ◽  
O.O. Johnson ◽  
G.A. Ayoola
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Green Synthesis ◽  
Antimicrobial Activities ◽  
Cu Nanoparticles ◽  
E Coli ◽  
Spondias Mombin ◽  
Ft Ir ◽  
Uv Visible

Background: Nanotechnology offers an advantage as a green route for synthesis of metal nanoparticles (NPs) with plant extracts as capping agent. Spondias mombin is a fruit-bearing tree and its leaf extracts have been reported to possess anxiolytic, hypoglycaemic, antiepileptic, antipsychotic, sedative, antioxidant, and antimicrobial properties. The objective of the study is to determine the antibacterial potential of a simple non-toxic product of green synthesis of metallic (Ag, Zn and Cu) nanoparticles using the leaf of Spondias mombin aqueous extracts (SMAE) as a reducing and capping agents of the metal ions.Methodology: Nanoparticles were characterized by UV visible spectrophotometeric analysis, Fourier Transform Infra-Red (FT-IR) spectrophotometer and scanning electron microscope (SEM). Antimicrobial activities of synthesized NPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were determined by agar well diffusion technique.Results: The synthesized NPs varied in colour from dark brown to green and appears dominantly spherical, occasionally rod or triangular shaped with size ranging from 65-90 nm. UV spectroscopy absorption spectra of Ag, Zn and Cu NPs had absorbance peak at 267, 262 and 765 nm respectively. FT-IR spectrometry of Zn NP, Cu NP, and SMAE gave wave number ranging from 895.71-3320.67, 747.02-3225.45 and 658.25-3674.49 respectively. FT-IR analysis showed that SMAE acted as reducing and stabilizing agent while the NPs exhibited lower energy absorption band when compared to the plant extract. The NPs demonstrated higher antimicrobial activities against S. aureus than Ps. aeruginosa and E. coli. The antimicrobial activity was higher with copper NP than Ag and Zn NPs, and also higher than SMAE.Conclusion: The result from this study presents an indication for an alternative means for development of novel antimicrobial agents for clinical and biotechnological applications.Keywords: synthesis, nanoparticles, FT-IR, UV-visible spectrophotometry, antibacterial activitiesFrench Title: Synthèse verte de nanoparticules d'Ag, de Zn et de Cu à partir d'extrait aqueux de feuilles de Spondias mombin et évaluation de leur activité antibactérienneContexte: La nanotechnologie offre un avantage en tant que voie verte pour la synthèse de nanoparticules métalliques (NP) avec des extraits de plantes comme agent de coiffage. Spondias mombin est un arbre fruitier et ses extraits de feuilles possèdent des propriétés anxiolytiques,  hypoglycémiques, antiépileptiques, antipsychotiques, sédatives,  antioxydantes et antimicrobiennes. L’objectif de l’étude est de déterminer le potentiel antibactérien d’un simple produit non toxique de synthèse verte de nanoparticules métalliques (Ag, Zn et Cu) à l’aide de la feuille d’extraits aqueux de Spondias mombin (SMAE) en tant qu’agent réducteur et coiffant de la ions métalliques.Méthodologie: Les nanoparticules ont été caractérisées par analyse  spectrophotométrique UV visible, spectrophotomètre à transformée de Fourier infrarouge (FT-IR) et microscope électronique à balayage (MEB). Lesactivités antimicrobiennes de NP synthétisées contre Escherichia coli, Staphylococcus aureus et Pseudomonas aeruginosa ont été déterminées par une technique de diffusion sur puits d’agar.Résultats: Les NP synthétisés ont une couleur allant du brun foncé au vert et apparaissent principalement sphériques, parfois en bâtonnets ou en triangles, avec des tailles allant de 65 à 90 nm. Les spectres  d'absorption par spectroscopie UV des NP Ag, Zn et Cu présentaient des pics  d'absorbance à 267, 262 et 765 nm respectivement. La spectrométrie FT-IR de Zn NP, Cu NP et SMAE a donné un nombre d'onde allant de 895,71 à 3320,67, 747,02 à 3225,45 et 658,25 à 3674,49 respectivement. L'analyse FT-IR a montré que le SMAE agissait en tant qu'agent réducteur et  stabilisant, alors que les NP présentaient une bande d'absorption d'énergie inférieure à celle de l'extrait de plante. Les NP ont démontré des activités antimicrobiennes plus élevées contre S. aureus que Ps. aeruginosa et E. coli. L'activité antimicrobienne était plus élevée avec les NP en cuivre que dans les NP Ag et Zn, et également supérieure à celle du SMAE.Conclusion: le résultat de cette étude présente une indication d'un autre moyen de développement de nouveaux agents antimicrobiens pour des applications cliniques et biotechnologiques.Mots-clés: synthèse, nanoparticules, FT-IR, spectrophotométrie UV-visible, activités antibactériennes 

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