ASSESSMENT OF VIRULENCE FACTORS OF CANDIDIAL SPECIES AND ITS BIOTYPING IN ORAL POTENTIALLY MALIGNANT DISORDERS

2021 ◽  
pp. 51-54
Author(s):  
Afreen Jan ◽  
Anshi Jain ◽  
Rajvir Singh ◽  
Devi Charan Shetty ◽  
Saurabh Juneja
Keyword(s):  
Virulence Factors ◽  
Germ Tube ◽  
Lipolytic Activity ◽  
Study Groups ◽  
Control Group ◽  
Study Group ◽  
Oral Potentially Malignant Disorders ◽  
Tube Test ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

The aim of the study was to evaluate the virulence factors of candidal species and its biotypes associated with Oral Potentially Malignant Disorders. Materials and methods included that the present study comprised of red and white lesions (40) and normal healthy control group (5) individuals. Oral swabs were taken from representative area of the lesion to be used for both cytological, microbiological and biochemical tests. Samples were inoculated for fungal growth in Sabouraud Dextrose Agar and culture-positive samples had undergone for the germ tube test, chlymadospore formation and chrome agar test. The virulence factors were conrmed by Lipolytic, proteolytic, protinease and phospholipase tests. Anova test, Kruskall Wallis test and Post Hoc test were the statistical analysis used. The result showed that in this study group no candidial growth was found in control group, study group showed positive germ tube test formation, chlymadospore formation and chrom agar test. All positive isolates produced the four virulence factors with higher levels in candida albican isolates. The hemolytic activity in leukoplakia was found to be more virulent followed by Protienase activity amongst the group and within the study groups. Phospholipase activity amongst the study groups was found more virulent in Oral squamous cell carcinoma. Lipolytic activity was found more aggressive in Denture induced stomatitis amongst all the study group. It can be concluded that in this study there is a close association of Candida species with Oral potentially malignant disorders. The correlation of virulence properties of Candida with potentially malignant disorders has led to its role in its invasive potential.

SALIVARY CORTISOL LEVELS TO ESTABLISH A CAUSE AND EFFECT RELATIONSHIP IN PROGRESSION OF ORAL POTENTIALLY MALIGNANT DISORDERS

2021 ◽  
pp. 4-7
Author(s):  
Shefali Shefali ◽  
Saurabh Juneja ◽  
Anshi Jain ◽  
Devi Charan Shetty ◽  
Nikita Gulati
Keyword(s):  
Salivary Cortisol ◽  
Study Groups ◽  
Oral Potentially Malignant Disorders ◽  
Group Iii ◽  
Complex Interactions ◽  
Group I ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Elisa Technique ◽  
Cortisol Levels

The progression and development of OPMDs is inuenced by a multitude of factors which include complex interactions between physiological, psychological, behavioral and social factors. The persistent activation of HPA axis through tobacco usage probably impairs immune response and has a role in progression of OPMDs. The quantication of salivary cortisol facilitates the assessment of nicotine impact on the oral mucosa and in the progression of OPMDs. This study was undertaken to estimate the salivary cortisol levels in the OPMDs with and without habits thereby signifying the importance of salivary cortisol in the causation of disease or as an effective biomarker for disease progression during the pathogenetic process of the disease. Salivary cortisol levels were estimated by ELISA technique in 29 cases of differing grades of oral potentially malignant disorders consisting of individuals with habit and lesions (Group I), 32 cases of individuals having habit without lesions (Group II) and 3 cases of individuals having lesion without habit (Group III) and 8 cases with neither habit nor lesions (Group IV). Salivary cortisol levels were correlated within the different study groups and were analyzed using SPSS (version 20). Salivary cortisol levels were raised in group I as compared to all other groups. Clarity in the present study has been achieved that salivary cortisol levels can be researched to the causation of the disease as an important step forward. This study could open up newer avenues in understanding the pathogenetic mechanisms in Oral Potentially malignant disorders.

scholarly journals ORAL POTENTIALLY MALIGNANT DISORDERS ASSOCIATED WITH BETEL QUID CHEWING HABIT IN SAMOSIR ISLAND, NORTH SUMATRA INDONESIA

2017 ◽  
Vol 20 (2) ◽  
pp. 72-79
Author(s):  
Indri Lubis ◽  
Ameta Primasari ◽  
Sayuti Hasibuan
Keyword(s):  
Health Centre ◽  
Betel Quid ◽  
Control Group ◽  
Oral Potentially Malignant Disorders ◽  
Public Health Centre ◽  
Increased Risk ◽  
Betel Quid Chewing ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
North Sumatra

Oral potentially malignant disorders have been associated with a betel quid chewing habit. To date, betel quid chewing has a major social and cultural role in the society of Samosir Island, North Sumatra, Indonesia. The purpose of the present study was to evaluate the occurrence of oral potentially malignant disorders associated with the habit of betel quid chewing in the society of Samosir Island, North Sumatra, Indonesia. This case-control study was conducted on all betel quid chewers in the working area of the Ambarita Public Health Centre in Samosir Regency, North Sumatra, Indonesia. All subjects were examined clinically for the presence of any oral lesions and interviewed for their betel quid chewing habit. Chi-square and Fisher’s exact tests were used to analyze the relationship between variables. Among the 51 subjects recruited in the study, 28 subjects suffered from oral potentially malignant disorders who had oral potentially malignant disorders such as submucous fibrosis and/or leukoplakia (the case group) whereas 23 subjects showed no clinically detectable oral potentially malignant disorders (the control group). An increased risk of oral potentially malignant disorders was associated with the habit of chewing areca nut and tobacco (OR=1.600; p=0.542), the duration of betel quid chewing more than 25 years (OR=4.379; p=0.023), and the frequency of betel quid chewing more than 6 times/day (OR=4.800; p=0.021). In conclusion, oral potentially malignant disorders were associated with chewing betel quid habit in the society of Samosir Island, North Sumatra, Indonesia.

scholarly journals Expression of Interleukin-1ß and Interleukin-8 in Oral Potentially Malignant Disorders and Carcinomas

2021 ◽  
Vol 2 ◽  
Author(s):  
Jeaneth Lopez-Labady ◽  
Ronell Bologna-Molina ◽  
Mariana Villarroel-Dorrego
Keyword(s):  
Oral Mucosa ◽  
Study Groups ◽  
Normal Mucosa ◽  
Interleukin 8 ◽  
Oral Potentially Malignant Disorders ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Oral Epithelial ◽  
Oral Lichen

Objective: To evaluate interleukin-1ß (IL-1ß) and interleukin-8 (IL-8) epithelial expressions in potentially malignant disorders of the oral mucosa as malignant predictive markers.Study design: About 55 tissues embedded in paraffin, comprising 15 oral lichen planus (OLP) lesions, 15 leukoplakias, 15 oral squamous cell carcinomas (OSCC), and 10 samples of normal oral mucosa were included in the study. IL-1ß and 8 expressions were assessed by immunohistochemistry using antibodies antihuman IL-1ß human (sc-7884, Santa Cruz® H-153) and antihuman IL-8 (ab7747, abcam®). The number of positive cells was compared using Student's t-test. Any p-value < 0.05 was considered statistically significant.Results: Nuclear and cytoplasmatic keratinocyte staining were positive for both cytokines in all study groups. However, a statistically significant decrease was observed within all cases compared to normal mucosa, both staining for IL-1β and 8. Moreover, IL-8 showed significant differences between OLP and leukoplakia, and when compared to OSCC.Conclusions: Oral epithelial expression of IL-1β and 8 seems to decrease when the malignant transformation of the oral mucosa increases.

scholarly journals Study of the TP53 codon 72 polymorphism in oral cancer and oral potentially malignant disorders in Argentine patients

Tumor Biology ◽  
2017 ◽  
Vol 39 (5) ◽  
pp. 101042831769911 ◽  
Author(s):  
Ana Maria Zarate ◽  
Julieta Don ◽  
Dante Secchi ◽  
Andres Carrica ◽  
Fernanda Galindez Costa ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Low Frequency ◽  
Cross Sectional Study ◽  
Control Group ◽  
Oral Potentially Malignant Disorders ◽  
Cross Sectional ◽  
Codon 72 Polymorphism ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Tp53 Codon 72 Polymorphism

The aim of this work was to evaluate the prevalence of TP53Arg72Pro mutations and their possible relationship with oral carcinoma and oral potentially malignant disorders in Argentine patients. A cross-sectional study was performed on 111 exfoliated cytologies from patients with oral cancer (OC), oral potentially malignant disorders (OPMD) and controls. The TP53Arg72Pro mutations were determined using conventional PCR. We evaluated univariate and multivariate study variables, setting p < 0.05. We found: (a) a low frequency of Pro72 variant in control group and a high frequency in OC and OPMD, as well in OC and oral leukoplakia (OL) diagnosis; (b) multivariate association among the TP53CC genotype and females over 45 years with no tobacco nor alcohol habits with oral lichen planus pathology; (c) multivariate association between the TP53GC genotype and males with alcohol and tobacco habits and OC and OL pathologies. Our results showed that the wild-type Arg72variant was related to control patients and Pro72variant was related to OC and OPMD, in Argentine patients.

Usefulness of protein-based salivary markers in the diagnosis of oral potentially malignant disorders: A systematic review and meta-analysis

2021 ◽  
pp. 1-13
Author(s):  
Esteban Alexis Arroyo ◽  
Sergio Piñeiro Donís ◽  
Cintia M. Chamorro Petronacci ◽  
Monica G. Oliveira Alves ◽  
Xabier Marichalar Mendía ◽  
...  
Keyword(s):  
Meta Analysis ◽  
Control Group ◽  
Cytokeratin 19 ◽  
Histopathological Diagnosis ◽  
Oral Potentially Malignant Disorders ◽  
Quantitative Expression ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Diagnostic Capacity ◽  
Meta Analyses

By using a meta-analytical approach, this study aimed to analyse the diagnostic capacity of protein-based biomarkers in saliva for the differential diagnosis of oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) from healthy individuals as control group (HCG). Articles on protein-based biomarkers in saliva, which provided quantitative expression in individuals with clinical and histopathological diagnosis of OPMD or oral leukoplakia (OL) were considered eligible. Searches were conducted in eight electronic databases. The methodological quality was assessed using the Quality Assessment of Diagnostic Studies tool (QUADAS-2). Functional analysis was also performed. Meta-analyses were performed using the OpenMeta tool (Analyst). Meta-analysis was possible for 4 of the 11 biomarkers studied. Only the carcinoembryonic antigen (CEA) and the soluble fragment of cytokeratin 19 (CYFRA21) were significant for the OSCC/OPMD subgroup, both with a very low heterogeneity. CEA had an OE = 25.854 (CI95%: 13.215–38.492, p< 0.001, I2 = 0) and CYFRA21 had an OE = 9.317 (CI95%: 9.014–9.619, p< 0.001, I2 = 0). For the OPMD/HCG subgroup, only CYFRA21 was significant, with an OE = 3.679 (CI95%: 0.663–6.696, p= 0.017) although with high heterogeneity (I2 = 91.24). The CEA and CYFRA21 markers proved very useful when differentiating OSCC from OPMD. The CYFRA21 was the only protein that was capable of distinguishing between OPMD and healthy controls.

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