SALIVARY CORTISOL LEVELS TO ESTABLISH A CAUSE AND EFFECT RELATIONSHIP IN PROGRESSION OF ORAL POTENTIALLY MALIGNANT DISORDERS

2021 ◽  
pp. 4-7
Author(s):  
Shefali Shefali ◽  
Saurabh Juneja ◽  
Anshi Jain ◽  
Devi Charan Shetty ◽  
Nikita Gulati
Keyword(s):  
Salivary Cortisol ◽  
Study Groups ◽  
Oral Potentially Malignant Disorders ◽  
Group Iii ◽  
Complex Interactions ◽  
Group I ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Elisa Technique ◽  
Cortisol Levels

The progression and development of OPMDs is inuenced by a multitude of factors which include complex interactions between physiological, psychological, behavioral and social factors. The persistent activation of HPA axis through tobacco usage probably impairs immune response and has a role in progression of OPMDs. The quantication of salivary cortisol facilitates the assessment of nicotine impact on the oral mucosa and in the progression of OPMDs. This study was undertaken to estimate the salivary cortisol levels in the OPMDs with and without habits thereby signifying the importance of salivary cortisol in the causation of disease or as an effective biomarker for disease progression during the pathogenetic process of the disease. Salivary cortisol levels were estimated by ELISA technique in 29 cases of differing grades of oral potentially malignant disorders consisting of individuals with habit and lesions (Group I), 32 cases of individuals having habit without lesions (Group II) and 3 cases of individuals having lesion without habit (Group III) and 8 cases with neither habit nor lesions (Group IV). Salivary cortisol levels were correlated within the different study groups and were analyzed using SPSS (version 20). Salivary cortisol levels were raised in group I as compared to all other groups. Clarity in the present study has been achieved that salivary cortisol levels can be researched to the causation of the disease as an important step forward. This study could open up newer avenues in understanding the pathogenetic mechanisms in Oral Potentially malignant disorders.

ASSESSMENT OF VIRULENCE FACTORS OF CANDIDIAL SPECIES AND ITS BIOTYPING IN ORAL POTENTIALLY MALIGNANT DISORDERS

2021 ◽  
pp. 51-54
Author(s):  
Afreen Jan ◽  
Anshi Jain ◽  
Rajvir Singh ◽  
Devi Charan Shetty ◽  
Saurabh Juneja
Keyword(s):  
Virulence Factors ◽  
Germ Tube ◽  
Lipolytic Activity ◽  
Study Groups ◽  
Control Group ◽  
Study Group ◽  
Oral Potentially Malignant Disorders ◽  
Tube Test ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

The aim of the study was to evaluate the virulence factors of candidal species and its biotypes associated with Oral Potentially Malignant Disorders. Materials and methods included that the present study comprised of red and white lesions (40) and normal healthy control group (5) individuals. Oral swabs were taken from representative area of the lesion to be used for both cytological, microbiological and biochemical tests. Samples were inoculated for fungal growth in Sabouraud Dextrose Agar and culture-positive samples had undergone for the germ tube test, chlymadospore formation and chrome agar test. The virulence factors were conrmed by Lipolytic, proteolytic, protinease and phospholipase tests. Anova test, Kruskall Wallis test and Post Hoc test were the statistical analysis used. The result showed that in this study group no candidial growth was found in control group, study group showed positive germ tube test formation, chlymadospore formation and chrom agar test. All positive isolates produced the four virulence factors with higher levels in candida albican isolates. The hemolytic activity in leukoplakia was found to be more virulent followed by Protienase activity amongst the group and within the study groups. Phospholipase activity amongst the study groups was found more virulent in Oral squamous cell carcinoma. Lipolytic activity was found more aggressive in Denture induced stomatitis amongst all the study group. It can be concluded that in this study there is a close association of Candida species with Oral potentially malignant disorders. The correlation of virulence properties of Candida with potentially malignant disorders has led to its role in its invasive potential.

scholarly journals Expression of Interleukin-1ß and Interleukin-8 in Oral Potentially Malignant Disorders and Carcinomas

2021 ◽  
Vol 2 ◽  
Author(s):  
Jeaneth Lopez-Labady ◽  
Ronell Bologna-Molina ◽  
Mariana Villarroel-Dorrego
Keyword(s):  
Oral Mucosa ◽  
Study Groups ◽  
Normal Mucosa ◽  
Interleukin 8 ◽  
Oral Potentially Malignant Disorders ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Oral Epithelial ◽  
Oral Lichen

Objective: To evaluate interleukin-1ß (IL-1ß) and interleukin-8 (IL-8) epithelial expressions in potentially malignant disorders of the oral mucosa as malignant predictive markers.Study design: About 55 tissues embedded in paraffin, comprising 15 oral lichen planus (OLP) lesions, 15 leukoplakias, 15 oral squamous cell carcinomas (OSCC), and 10 samples of normal oral mucosa were included in the study. IL-1ß and 8 expressions were assessed by immunohistochemistry using antibodies antihuman IL-1ß human (sc-7884, Santa Cruz® H-153) and antihuman IL-8 (ab7747, abcam®). The number of positive cells was compared using Student's t-test. Any p-value < 0.05 was considered statistically significant.Results: Nuclear and cytoplasmatic keratinocyte staining were positive for both cytokines in all study groups. However, a statistically significant decrease was observed within all cases compared to normal mucosa, both staining for IL-1β and 8. Moreover, IL-8 showed significant differences between OLP and leukoplakia, and when compared to OSCC.Conclusions: Oral epithelial expression of IL-1β and 8 seems to decrease when the malignant transformation of the oral mucosa increases.

scholarly journals Genetic and Proteinic Linkage of MAO and COMT with Oral Potentially Malignant Disorders and Cancers of the Oral Cavity and Pharynx

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3268
Author(s):  
Ping-Ho Chen ◽  
Yen-Yun Wang ◽  
Ting-Hsun Lan ◽  
Leong-Perng Chan ◽  
Shyng-Shiou Yuan
Keyword(s):  
Oral Cavity ◽  
Potential Role ◽  
Nucleotide Polymorphisms ◽  
Oral Potentially Malignant Disorders ◽  
Clinical Biomarkers ◽  
Group I ◽  
Increased Risk ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Betel quid (BQ), a group I human carcinogen, strongly contributes to an increased risk of oral potentially malignant disorders (OPMD) and cancers of the oral cavity and pharynx. This study was conducted to discover whether monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT) variants play a potential role in the risk assessment of oral cavity and pharynx cancers and OPMD, particularly among BQ users. We applied a case–control study to confirm the polymorphism of MAO and COMT using single-nucleotide polymorphisms. We used qRT-PCR, Western blotting, and immunohistochemistry (IHC) to determine MAO and COMT expression. Carriers of the MAOA rs6323 G-allele, MAOB rs6324 G-allele, and COMT rs4633 C/C-genotype had a prominently increased risk of oral cavity and pharynx cancers (AOR = 56.99; p < 0.001). Compared to adjacent noncancerous tissues, a significant downregulation of MAO and COMT expression was exhibited in cancerous tissues (p < 0.01). Furthermore, in different cell models, MAO and COMT expression was significantly downregulated with an increased dose of arecoline (p < 0.01). In personalized preventive medicine for oral and pharyngeal cancers, our findings are the first to demonstrate the potential role of lower MAO and COMT expression levels, with the risk polymorphisms utilized as clinical biomarkers.

Identification and Quantification of “Cancer Stem Cells” in Potentially Malignant and Malignant Disorders of Head and Neck Region: A Flow-Cytometric Study

2021 ◽  
Vol 13 (3) ◽  
pp. 189-197
Author(s):  
Vathsala Naik ◽  
Manjunatha M Venkataswamy ◽  
Ruthu Nagraj ◽  
Ganga GK ◽  
Gaurav .
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Clinical Stage ◽  
P Value ◽  
Surface Markers ◽  
Group Iii ◽  
Group I ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Group Ii

Background: Cancer stem cells (CSCs) are subpopulation of cells existent in a cancerous mass of cells. These CSCs hijack the properties of stem cells like self-renewal as well as being resistant to any conventional cancer therapies. The objective of this study was to identify and quantify the presence of these CSCs by using surface markers CD44, CD133, and ALDH1 among three groups of subjects who were age and gender matched (Normal controls, Oral cancers, Potentially malignant disorders). Methodology: This study was conducted in a sample of 108 subjects who were divided into three groups: Group I- Controls (C), Group II- Oral cancer (OC), Group III- Potentially Malignant Disorders (PMDs). Among them, 40 subjects each were present in Group I & II and 28 subjects were included in group III, and they were respectively diagnosed histopathologically as OC and PMDs. The identification of the sub-population of CSCs by means of above mentioned surface markers was done using Flow cytometry. Results interpretation: Non-parametric tests were applied. Median age limit was 59 years in group II, which was higher than Group I or Group III subjects. p-value was 0.002* which was significant. Group I included 19 females (47.5%) and 21 males (52.5%). In Group II, 18 subjects were females (45%) and 22 were males (55%), and in Group III, 17 subjects were females (60.7%) and 11 were males (39.3%). p-value was 0.409, which was not very significant. In group II, 23 subjects (57.5%) were in clinical stage 2, 11 (27,5%) were in clinical stage 1 and six (15%) were in clinical stage 3. Histopathologically in group II, 15 (37.5%) were in grade I, 20 (50%) were in grade II and five (12.5%) were in grade III. Results of the three groups were compared and correlated regarding the presence of cancer stem cells based on the surface markers CD44, CD133 and ALDH1. Unlike other similar studies, our study showed no statistically significant presence of CD44, ALDH1 positive cells, but only CD133 was slightly significant. Inference: The results of our study showed no statistically significant evidence in identification of the presence of cancer stem cells in the oral cancers as well as potentially malignant disorders based on the presence of surface markers.

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