scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Rizka Matoka ◽  
Olivia Waworuntu ◽  
Fredine Rares

Abstract: Nosocomial infection or Health-care Associated Infection (HAIs) according to the WHO in 2011 are infection to patient acquired during a hospital, after ≥48 hours and ≤30 days after out of the hospital. Factor of infection can be differentiated between patients, nurses, doctors, hospital employed, hospital environment and patient visitor or family. The purpose to know the pattern of aerobic bacteria that could potentially to involve nosocomial infection in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado. Type a descriptive observational research with cross sectional study approach. The sampling on the walls, floors, medical equipment, room furniture and the air in the IRDO Prof. Dr. R. D. Kandou Hospital Manado. The results of the 27 samples found bacteria Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%).Conclusion: The most aerobic bacteria in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado is Bacillus sp.(46,55%).Keywords: Emergency Obstetric and Gynecology, nosocomial infection, aerobic bacteria. Abstrak: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) adalah suatu instalasi penanggulangan awal penderita rawat darurat untuk mencegah kematian, dalam menangani kesehatan reproduksi wanita saat tidak hamil ataupun pada saat hamil, persalinan, dan nifas. Infeksi nosokomial atau Health-care Associated Infection (HAIs) menurut WHO 2011 merupakan infeksi yang didapat pasien selama di rumah sakit, setelah ≥ 48 jam dan ≤ 30 hari setelah keluar dari rumah sakit. Faktor terjadinya infeksi bisa dibebakan antar pasien, perawat, dokter, karyawan rumah sakit, lingkungan rumah sakit, dan pengunjung atau keluarga pasien. Tujuan untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian deskriptif observational dengan pendekatan studi cross sectional. Pengambilan sampel pada dinding, lantai, peralatan medis, perabotan ruangan, udara di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 27 sampel ditemukan bakteri Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%). Simpulan: Bakteri aerob di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus sp.(46,55%).Kata kunci: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO), Infeksi nosokomial, bakteri aerob

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Yulya Margaretha Longadi ◽  
Olivia Waworuntu ◽  
Standy Soeliongan

Abstract: Nosocomial infection or Hospital Acquired Infection (HAI) is an infection caused by bacteria, parasite, or virus in the hospital, infection occur at least 72 hours since hospitalized. This infection occurs due to lack of hygiene of the environment causing microorganism infection from environment to human, infection can also occur due to transmission of microorganism from one patient to other patients. Inpatients potentially have very high risk of nosocomial infection occur due to continuous requiring treatment for more than 24 hours. Purpose: To determine the existence of aerobic bacteria that could potentially be the source of transmission of nosocomial infection in Irina A RSUP Prof. Dr. R. D. Kandou Manado. Method: This research was descriptive with cross sectional approach. Fourteen samples were taken from the surface of medical equipment, bed, floor, and wall of the treatment room and eight samples were taken from the air. Identification of bacteria was performed by culture on agar medium, staining gram, and biochemical test. Result: Bacillus subtilis found in nine samples (41%), Serratia liquefaciens found in five samples (22,7%), Lactobacillus found in two samples (9,1%), Staphylococcus found in two samples (9,1%), Coccus Gram negative found in two samples (9,1%), Enterobacter aerogenes found in one sample (4,5%), and Enterobacter agglomerans found in one sample (4,5%). Conclusion: Bacillus subtilis is the most bacteria which had been found in this research.Keywords: Aerobic bacteria, Nosocomial infection, Irina A.Abstrak: Infeksi nosokomial atau Hospital Acquired Infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit, atau virus di rumah sakit, infeksi ini timbul sekurang-kurangnya 72 jam sejak masuk rumah sakit. Infeksi ini terjadi akibat kurang bersihnya lingkungan perawatan menyebabkan terjadinya infeksi mikroorganisme dari lingkungan ke manusia, infeksi juga dapat terjadi akibat berpindahnya mikroorganisme dari pasien yang satu ke pasien yang lain. Pasien ruang rawat inap mempunyai risiko yang sangat tinggi mendapatkan infeksi nosokomial, hal ini dikarenakan pasien pada ruang rawat inap memerlukan asuhan, perawatan dan pengobatan secara berkesinambungan lebih dari 24 jam. Tujuan: Untuk mengetahui adanya bakteri aerob yang berpotensi menjadi sumber penularan infeksi nosokomial di IRINA A RSUP Prof. Dr. R. D. kandou Manado. Metode: Metode penelitian yang digunakan adalah deskriptif dengan pendekatan cross sectional study. Sampel diambil dari 14 usapan permukaan peralatan medis, tempat tidur, lantai, dan dinding ruang perawatan dan delapan sampel dari udara ruang. Identifikasi bakteri dilakukan kultur pada media agar, pewarnaan gram, dan uji biokimia. Hasil: Bacillus subtilis ditemukan sebanyak sembilan sampel (41%), Serratia liquefaciens sebanyak lima sampel (22,7%), Lactobacillus sebanyak dua sampel (9,1%), Staphylococcus sebanyak dua sampel (9,1%), Kokus Gram negatif sebanyak dua sampel (9,1%), Enterobacter aerogenes sebanyak satu sampel (4,5%), dan Enterobacter agglomerans sebanyak satu sampel (4,5%). Kesimpulan: Bacillus subtilis merupakan bakteri yang paling banyak ditemukan.Kata Kunci: Bakteri aerob, Infeksi nosokomial, Irina A.


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Hilary G. Sarlin ◽  
Heriyannis Homenta ◽  
John Porotu'o

Abstract: Nosocomial infections are infections that arise during the patient was hospitalized and starting to show symptoms for a person being treated or after completion of treatment. Patients in the ICU may have a tendency to nosocomial infections. The purpose of the study is to identify and knowing the most common aerobic bacteria that cause nosocomial infections in the ICU RSAD Robert Wolter Monginsidi Teling Manado.This research is a descriptive cross sectional study. The study samplesare bacteria on the floor, walls, air, bed, and ECG in the ICU RSAD Teling Robert Wolter Monginsidi Manado. The results showed 26 samples out of 26 samples contained bacteria, Bacillus sp. eight samples (30.7%), Enterobacter aerogens six samples (23%), Staphylococcus sp. six samples (23%) and Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. each found as many as one sample (3.8%). Conclusion: The most common bacteria is Bacillus sp.Keywords: nosocomial infection, ICU, aerobic bacteria Abstrak: Infeksi nosokomial adalah Infeksi yang muncul selama penderita tersebut dirawat dirumah sakit dan mulai menunjukkan suatu gejala selama seseorang itu dirawat atau setelah selesai dirawat. Pasien yang berada di ruang ICU dapat mempunyai kecenderungan terkena infeksi nosokomial. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui bakteri aerob yang terbanyak penyebab infeksi nosokomial di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Desain penelitian ini bersifat deskriptif dengan studi cross sectional. Sampel penelitian ialah bakteri pada lantai, dinding, udara, tempat tidur, dan EKG di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Hasil penelitian memperlihatkan dari 26 sampel terdapat 26 sampel yang tumbuh yaitu, Bacillus sp. sebanyak delapan sampel (30,7%), Enterobacter aerogens ditemukan sebanyak enam sampel (23%), Staphylococcus sp. Ditemukan sebanyak enam sampel (23%) sedangkan Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. masing-masing ditemukan sebanyak satu sampel (3,8%). Simpulan: bakteri yang paling banyak ditemukan adalah Bacillus sp. Kata kunci: infeksi nosokomial, ICU, bakteri aerob


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Gabriella F.A. Kambey ◽  
Heriyannis Homenta ◽  
John Porutu'o

Abstract: Infection acquired in the hospital (nosocomial infection) is defined as an infection that does not exist or is in the incubation period at the time of hospital admission and is associated with health care. Nosocomial infection occurs as a result of interaction of several factors, among others, the types of microbes in the hospital environment, nursing actions that allow microbial intervention into the body through the skin and mucous membranes, interactions among patients, hospital workers, equipment and the environment as well as an increase of opportunistic microbial resistance. This study aimed to determine the pattern of aerobic bacteria that were potential to cause nosocomial infections in the delivery room of Prof. Dr. R. D. Kandou Hospital Manado. This was a prospective descriptive study. Samples were obtained from walls, floors, medical equipment, and the air in the delivery room at Prof. Dr. R. D. Kandou Manado. The results showed that of 22 samples, there were several types of bacteria as follows: Bacillus subtillis (40.1%), Enterobacter aerogenes (18.2%), Gram-negative cocci (18.2%), Serratia liquefaciens (13.6%), and Enterobacter cloacae (9.9%). Conclusion: The most common type of aerobic bacteria in the delivery room of Prof. Dr. R. D. Kandou Hospital Manado was Bacillus subtillis. Keywords: delivery room, nosocomial infections, aerobic bacteria Abstrak: Infeksi yang didapat di rumah sakit (nosokomial) didefinisikan sebagai infeksi yang belum ada atau sedang dalam masa inkubasi pada saat masuk rumah sakit dan infeksi yang berkaitan dengan perawatan kesehatan. Infeksi nosokomial terjadi sebagai akibat interaksi oleh beberapa faktor yaitu antara lain: adanya jenis-jenis mikroba di lingkungan rumah sakit, tindakan keperawatan yang memungkinkan mikroba intervensi ke dalam tubuh melalui kulit dan membran mukosa, interaksi antara pasien, pekerja rumah sakit, peralatan dan lingkungan, serta peningkatan resistensi mikroba oportunistik. Penelitian ini bertujuan untuk mengetahui pola bakteri aerob di dinding, lantai, peralatan medis, dan udara di kamar bersalin RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ini deskriptif dengan pendekatan prospektif. Sampel penelitian diambil dari dinding, lantai, peralatan medis dan udara di kamar bersalin RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 22 sampel mendapatkan bakteri yang tumbuh ialah Basillus subtillis (40,1%), Enterobacter aerogenes (18,2%), kokus Gram negatif (18,2%), Serratia liquefaciens (13,6%), dan Enterobacter cloacae (9,9%). Simpulan: Bakteri aerob di kamar bersalin Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus subtillis. Kata kunci: kamar bersalin, infeksi nosokomial, bakteri aerob


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Sitti N. Gobel ◽  
Fredine E.S. Rares ◽  
Heriyannis Homenta

Abstract: Nosocomial infection or Hospital Acquired infection (HAI) is caused by bacteria, parasites or viruses in hospitals and occurs after ≥48 hours of hospital admission. Emergency room is te starting place procedures and or medical measures so that the room IGD became one of the places that produces a lot of waste of medical procedures performed and as one of the places most likely to transmit the infection. This study was aimed to determine the sources and patterns of aerobic bacteria that could potentially cause nosocomial infections: in the walls, floors, medical equipment, and ambient air of Emergency Room Robert Wolter Mongisidi Hospital, Manado. This was a descriptive prospective study with a cross sectional design. Samples were bacteria in the walls, floors, medical equipment, and the ambient air of the room IGD in the emergency room, Robert Wolter Mongisidi Hospital, Manado. The results showed that of the 29 samples taken, one sample did not contain any bacteria. There were eight types of bacteria found: Bacillus sp (33%), Staphylococcus sp (27%) Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) and Klebsiella pneumoniae (2%). Conclusion: The most common bacteria was Bacillus sp.Keywords: IGD, nosocomial infections, patterns of aerobic bacteria Abstrak: Infeksi nosokomial atau Hospital Acquired infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit atau virus di rumah sakit, terjadi ≥ 48 jam sejak masuk rumah sakit. Ruangan IGD merupakan tempat awal pasien mendapatkan prosedur dan atau tindakan medis sehingga ruangan IGD menjadi salah satu tempat yang banyak menghasilkan limbah hasil prosedur dan tindakan medis yang dilakukan serta salah satu tempat yang paling mungkin menularkan infeksi. Penelitian ini bertujuan untuk mengetahui sumber dan pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial: di dinding, lantai, peralatan medis, dan udara di ruang IGD pada ruang gawat darurat medik RSAD Robert Wolter Mongisidi Manado. Jenis penelitian ialah deskriptif prospektif dengan desain otong lintang. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di ruang gawat darura tmedik RSAD Robert Wolter Mongisidi Manado. Hasil penelitian memperlihatkan dari 29 sampel yang diambil 1 sampel tidak terdapat bakteri. Terdapat 8 jenis bakteri yang ditemukan yaitu: Bacillus sp (33%), Staphylococcus sp (27%), Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) dan Klebsiella Pneumoniae (2%). Simpulan: Bakteri yang paling banyak ditemukan ialah Bacillus sp. Kata kunci: IGD, infeksi nosokomial, pola bakteri aerob


2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Tuhatelu N. Siti ◽  
Waworuntu O. ◽  
Porotu’o J.

Abstract: Diarrhea is defecate more often, more, with greater consistency mushy or watery than usual. Diarrhea is still a major health problem in children, especially in developing countries such as Indonesia. Sanitation and poor environmental hygiene are factors that result in high levels of diarrhea in children in Indonesia. Generally diarrhea caused by infection (± 90%), such as bacteria, viruses, parasites or fungi. The purpose of this study was to determine the pattern of aerobic bacteria that cause diarrhea in children in Hospital Robert Wolter Monginsidi Teling. Methods research study conducted by using a cross sectional study with prospective descriptive approach. Samples were taken from 20 faecal samples from children with diarrhea in Inpatient Hospital Robert Wolter Monginsidi Teling. Identification of bacteria carried by stool culture, gram staining and biochemical tests. In the results of the study showed Enterobacter aerogenes 4 samples (20%), Lactobacillus sp 3 samples (15%), Proteus vulgaris, Shigella and Staphylococcus sp sp 2 each sample (10%), Proteus mirabilis, Serratia liquefaciens, Serratia rubidae, Salmonella arisona, Escherechia coli, Streptococcus sp, Candida each 1 sample (5%). Conclusions on research most types of bacteria found were Enterobacter aerogenes.Keywords: aerobic bacteria, diarrhea, Enterobacter aerogeneAbstrak: Diare adalah buang air besar yang lebih sering, lebih banyak, dengan konsistensi yang lebih lembek atau encer dari biasanya. Diare masih merupakan masalah kesehatan utama pada anak, terutama di negara berkembang seperti Indonesia. Sanitasi dan kebersihan lingkungan yang buruk merupakan faktor yang menyebabkan masih tingginya tingkat kejadian diare pada anak Indonesia. Umumnya diare disebabkan oleh infeksi (± 90 %), seperti bakteri, virus, parasit atau jamur. Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri aerob penyebab diare pada anak di RSU Robert Wolter Monginsidi Teling. Metode penelitian penelitian yang dilakukan dengan menggunakan studi cross sectional dengan pendekatan deskriptif prospektif. Sampel diambil dari 20 sampel feses dari anak diare di Instalasi Rawat Inap RSU Robert Wolter Monginsidi Teling. Identifikasi bakteri dilakukan melalui kultur feses, pewarnaan gram dan uji biokimia. Pada hasil penelitian menunjukan Enterobacter aerogenes sebesar 4 sampel(20%), Lactobacillus sp 3 sampel (15%), Proteus vulgaris, Shigella sp dan Staphylococcus sp masing-masing 2 sampel( 10%), Proteus mirabilis ,Serratia Liquefaciens, Serratia rubidae, Salmonella arisona, Escherechia coli, , Streptococcus sp, Candida masing-masing 1 sampel (5%). Kesimpulan pada penelitian jenis bakteri terbanyak ditemukan adalah Enterobacter aerogenes.Kata Kunci: bakteri aerob, diare, enterobacter aerogenes


2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Malindra Saleh ◽  
Fredine E. S . Rares ◽  
Standy Soeliongan

Abstract: Nosocomial infection is an infection which occurs in the hospital caused by microbacteria from the hospital. Infection in neonatal is a serious issue in each hospital until today. Neonatal Intensive Care Unit (NICU) is a care unit for newborn who needs special care. Nosocomial infection is often happening to babies in NICU. The objective is to determine the pattern of aerob bacteria causes nosocomial infection in Neonatal Intensive Care Unit (NICU) BLU RSUP Prof. Dr. R. D. Kandou Manado. The research used descriptive method with prospective study. The amount of samples are 30 and was taken according to category of care room, room instruments, medical equipments and air. Based on the results, there are 11 species of bacterias included Bacillus subtilis 13 samples (43,3%), Serratia liquefaciens 4 samples (13,3%), Lactobacillus 3 samples (10%), Enterobacter agglomerans 2 samples (6,7%) and Klebsiella pneumoniae 2 samples (6,7%), Proteus mirabilis 1 sample (3,3%), Proteus vulgaris 1 sample (3,3%), Streptococcus non hemoliticus 1 sample (3,3%), Diplococcus 1 sample (3,3%), gram-positive Coccus 1 sample (3,3%) and gram-negative Coccus 1 sample (3,3%).Keywords: nosocomial infection, NICU, aerob bacteriaAbstrak: Infeksi nosokomial adalah infeksi yang terjadi di rumah sakit oleh kuman yang berasal dari rumah sakit. Infeksi nosokomial yang terjadi pada bayi baru lahir sampai saat ini merupakan masalah yang serius disetiap rumah sakit. Neonatal Intensive Care Unit (NICU) merupakan unit perawatan untuk bayi baru lahir yang memerlukan perawatan khusus. Infeksi nosokomial sering terjadi terutama pada bayi yang mendapat perawatan di NICU. Tujuan dari penelitian ini yaitu untuk mengetahui pola bakteri aerob penyebab infeksi nosokomial pada ruangan Neonatal Intensive Care Unit (NICU) BLU RSUP Prof. Dr. R. D Kandou Manado. Penelitian ini menggunakan metode deskriptif dengan pendekatan studi prospektif. Sampel yang diteliti berjumlah 30 sampel dan di ambil berdasarkan kategori ruang perawatan, perabotan ruangan, peralatan medis dan udara. Berdasarkan hasil penelitian ditemukan 11 spesies bakteri yaitu Bacillus subtilis 13 sampel (43,3%), Serratia liquefaciens 4 sampel (13,3%), Lactobacillus 3 sampel (10%), Enterobacter agglomerans 2 sampel (6,7%) dan Klebsiella pneumoniae 2 sampel (6,7%), Proteus mirabilis 1 sampel (3,3%), Proteus vulgaris 1 sampel (3,3%), Streptococcus non hemolitikus 1 sampel (3,3%), Diplokokus 1 sampel (3,3%), Kokus gram positif 1 sampel (3,3%) dan Kokus gram negatif 1 sampel (3,3%).Kata kunci: infeksi nosokomial, NICU, bakteri aerob


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Marshall C. Waworuntu ◽  
Fredine E.S. Rares ◽  
Heriyannis Homenta

Abstract: Nosocomial infection is an infection which occurs in the hospital caused by microbateria from the hospital. Nosocomial infections that occur in the newborn is a serious problem in every hospital. Neonatal Intensive Care Unit (NICU) is a care unit for newborn who needs special care. Nosocomial infections often occur, especially in infants hospitalized in NICU. The purpose of this research is to determine the pattern of aerobic bacteria that could potentially cause nosocomial infections in NICU RSAD Robert Wolter Monginsidi Manado. The research used descriptive cross sectional method. The amount of the samples are 29 and was taken according to category of the treatment room, room instruments, medical equipments and air. Based on the results, there are 9 species of bacterias included Enterobacter cloacae (27%), Staphylococcus sp (17%), Bacillus subtilis (13%), Klebsiella pneumoniae (13%), Serratia liquefaciens (10%), Serratia marcescens (7%), Enterobacter aerogenes (7%), Neisseria sp (3%) and Escherichia coli (3%). The most bacteria that could potentially cause nosocomial infections were Enterobacter cloacae.Keywords: nosocomial infections, NICU, aerob bacteria Abstrak: Infeksi nosokomial adalah suatu infeksi yang didapat atau terjadi saat pasien dirawat di rumah sakit oleh kuman yang berasal dari rumah sakit. Infeksi nosokomial yang terjadi pada bayi baru lahir merupakan masalah yang serius disetiap rumah sakit. Neonatal Intensive Care Unit (NICU) merupakan unit perawatan untuk bayi baru lahir yang memerlukan perawatan khusus. Infeksi nosokomial sering terjadi terutama pada bayi yang dirawat di NICU. Tujuan penelitian ini yaitu untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan NICU RSAD Robert Wolter Mongisidi Manado. Penelitian ini menggunakan metode deskriptif dengan pendekatan potong lintang (cross sectional). Sampel yang diteliti berjumlah 29 sampel dan di ambil berdasarkan kategori ruang perawatan, perabotan ruangan, peralatan medis dan udara. Berdasarkan hasil penelitian ditemukan 9 spesies bakteri yaitu Enterobacter cloacae (27%), Staphylococcus sp (17%), Bacillus subtilis (13%), Klebsiella pneumoniae (13%), Serratia liquefaciens (10%), Serratia marcescens (7%), Enterobacter aerogenes (7%), Neisseria sp (3%) dan Escherichia coli (3%). Bakteri yang paling banyak ditemukan adalah Enterobacter cloacae. Kata kunci: infeksi nosokomial, NICU, bakteri aerob


2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lisa E. Ritto ◽  
Standy Soeliongan ◽  
Fredine E. S. Rares

Abstract: The situation in countries with limited resources, are radically different in almost every aspect, making pregnant women in developing countries prone to a higher risk of getting nosocomial infections after childbirth than their counterparts in developed countries. One of the most common cause of nosocomial infection is bacterial. The aim of this study was to determine the pattern of aerobic bacteria that could potentially cause nosocomial infection in delivery room RSAD Robert Wolter Monginsidi Manado and followed by isolation and identification of bacteria. This research uses descriptive method with prospective approach. Samples were taken by acquiring swabs from the walls, floor, room furniture, medical equipment, and air as much as 25 samples. As a result, there are Lactobacillus sp. 7 samples (28%), Enterobacter agglomerans 7 samples (28%), Bacillus subtilis 6 samples (24%), Staphylococcus epidermidis 3 samples (12%), Serratia liquefaciens 1 samples (4%) and Enterobacter cloacae 1 samples (4%). This study found the most bacteria are Lactobacillus sp. and Enterobacter agglomerans.Keywords: aerobic bacteria, nosocomial infection, delivery room.Abstrak: Situasi di negara-negara dengan sumber-sumber yang terbatas, secara radikal berbeda hampir dalam setiap aspek membuat perempuan hamil di negara-negara sedang berkembang dihadapkan kepada resiko lebih tinggi untuk mendapatkan infeksi nosokomial sesudah persalinan daripada mitranya di negara-negara maju. Salah satu penyebab tersering infeksi nosokomial adalah bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada kamar bersalin RSAD Robert Wolter Mongisidi Manado yang kemudian dilakukan isolasi dan identifikasi bakteri. Penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan prospektif. Sampel diambil secara usapan dari dinding, lantai, perabotan ruangan, peralatan medis, dan udara sebanyak 25 sampel. Hasil penelitian ditemukan Lactobacillus sp. 7 sampel (28%), Enterobacter agglomerans 7 sampel (28%), Bacillus subtilis 6 sampel (24%), Staphylococcus epidermidis 3 sampel (12%), Serratia liquefaciens 1 sampel (4%) dan Enterobacter cloacae 1 sampel (4%). Kesimpulan dari penelitian ini bakteri terbanyak yang ditemukan adalah Lactobacillus sp. dan Enterobacter agglomerans.Kata kunci: bakteri aerob, infeksi nosokomial, kamar bersalin


2019 ◽  
Vol 2 ◽  
Author(s):  
Anggi Reza Pramesti ◽  
Sella Mustika ◽  
Nurul Habibah ◽  
Sofia Puspitarini ◽  
Meichica Serlie ◽  
...  

Banyaknya kegiatan Penambangan Emas Tanpa Ijin (PETI) di Indonesia menjadi sumber utama terjadinya pencemaran limbah merkuri. Merkuri merupakan salah satu jenis polutan logam berat yang bersifat toksik. Merkuri menimbulkan masalah serius pada kesehatan manusia karena dapat terakumulasi pada tubuh dan bersifat neurotoksin. Beberapa bakteri memiliki kemampuan resisten terhadap merkuri. Sejumlah bakteri yang resisten terhadap limbah merkuri telah diisolasi dari berbagai lokasi pertambangan emas di Indonesia. Beberapa bakteri lokal tersebut dirangkum dalam penelitian ini. Bakteri tersebut diantaranya yaitu : Bacillus sp., Escherichia coli, Enterobacter cloacea, Enterobacter aerogenes, Klebsiella pneumoniae, Alcaligenes sp., Pseudomonas sp., Enterobacter agglomerans, Proteus vugaris, Proteus mirabilis, Enterobacter hafniae, Kliebsella sp., Lactobacillus sp., Morganella morganii, Brevundimonas vesicularis, Nitrococcus mobilis, Fusobacterium aquatile, dan Fusobacterium necrogenes. Bakteri yang resisten terhadap merkuri tersebut dapat dimanfaatkan sebagai agen untuk remediasi wilayah yang tercemar limbah merkuri


2008 ◽  
Vol 52 (8) ◽  
pp. 2818-2824 ◽  
Author(s):  
Pattarachai Kiratisin ◽  
Anucha Apisarnthanarak ◽  
Chaitat Laesripa ◽  
Piyawan Saifon

ABSTRACT Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae have rapidly spread worldwide and pose a serious threat for health care-associated (HA) infection. We conducted molecular detection and characterization of ESBL-related bla genes, including bla TEM, bla SHV, bla CTX-M, bla VEB, bla OXA, bla PER, and bla GES, among 362 isolates of ESBL-producing E. coli (n = 235) and ESBL-producing K. pneumoniae (n = 127) collected from patients who met the definition of HA infection at two major university hospitals in Thailand from December 2004 to May 2005. The prevalence of ESBL-producing E. coli and ESBL-producing K. pneumoniae, patient demographics and the susceptibilities of these bacteria to various antimicrobial agents were described. A total of 87.3% of isolates carried several bla genes. The prevalence of bla CTX-M was strikingly high: 99.6% for ESBL-producing E. coli (CTX-M-14, -15, -27, -40, and -55) and 99.2% for ESBL-producing K. pneumoniae (CTX-M-3, -14, -15, -27, and -55). ISEcp1 was found in the upstream region of bla CTX-M in most isolates. Up to 77.0% and 71.7% of ESBL-producing E. coli and ESBL-producing K. pneumoniae, respectively, carried bla TEM; all of them encoded TEM-1. ESBL-producing K. pneumoniae carried bla SHV at 87.4% (SHV-1, -2a, -11, -12, -27, -71, and -75) but only at 3.8% for ESBL-producing E. coli (SHV-11 and -12). bla genes encoding VEB-1 and OXA-10 were found in both ESBL-producing E. coli (8.5% and 8.1%, respectively) and ESBL-producing K. pneumoniae (10.2% and 11.8%, respectively). None of the isolates were positive for bla PER and bla GES. Pulsed-field gel electrophoresis analysis demonstrated that there was no major clonal relationship among these ESBL producers. This is the first study to report CTX-M-3, CTX-M-27, CTX-M-40, SHV-27, SHV-71, and SHV-75 in Thailand and to show that CTX-M ESBL is highly endemic in the country.


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