scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di kamar bersalin RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Gabriella F.A. Kambey ◽  
Heriyannis Homenta ◽  
John Porutu'o
Keyword(s):  
Nosocomial Infection ◽  
Nosocomial Infections ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
The Body ◽  
Delivery Room ◽  
Hospital Environment ◽  
Aerobic Bacteria ◽  
Hospital Workers ◽  
Serratia Liquefaciens

Abstract: Infection acquired in the hospital (nosocomial infection) is defined as an infection that does not exist or is in the incubation period at the time of hospital admission and is associated with health care. Nosocomial infection occurs as a result of interaction of several factors, among others, the types of microbes in the hospital environment, nursing actions that allow microbial intervention into the body through the skin and mucous membranes, interactions among patients, hospital workers, equipment and the environment as well as an increase of opportunistic microbial resistance. This study aimed to determine the pattern of aerobic bacteria that were potential to cause nosocomial infections in the delivery room of Prof. Dr. R. D. Kandou Hospital Manado. This was a prospective descriptive study. Samples were obtained from walls, floors, medical equipment, and the air in the delivery room at Prof. Dr. R. D. Kandou Manado. The results showed that of 22 samples, there were several types of bacteria as follows: Bacillus subtillis (40.1%), Enterobacter aerogenes (18.2%), Gram-negative cocci (18.2%), Serratia liquefaciens (13.6%), and Enterobacter cloacae (9.9%). Conclusion: The most common type of aerobic bacteria in the delivery room of Prof. Dr. R. D. Kandou Hospital Manado was Bacillus subtillis. Keywords: delivery room, nosocomial infections, aerobic bacteria Abstrak: Infeksi yang didapat di rumah sakit (nosokomial) didefinisikan sebagai infeksi yang belum ada atau sedang dalam masa inkubasi pada saat masuk rumah sakit dan infeksi yang berkaitan dengan perawatan kesehatan. Infeksi nosokomial terjadi sebagai akibat interaksi oleh beberapa faktor yaitu antara lain: adanya jenis-jenis mikroba di lingkungan rumah sakit, tindakan keperawatan yang memungkinkan mikroba intervensi ke dalam tubuh melalui kulit dan membran mukosa, interaksi antara pasien, pekerja rumah sakit, peralatan dan lingkungan, serta peningkatan resistensi mikroba oportunistik. Penelitian ini bertujuan untuk mengetahui pola bakteri aerob di dinding, lantai, peralatan medis, dan udara di kamar bersalin RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ini deskriptif dengan pendekatan prospektif. Sampel penelitian diambil dari dinding, lantai, peralatan medis dan udara di kamar bersalin RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 22 sampel mendapatkan bakteri yang tumbuh ialah Basillus subtillis (40,1%), Enterobacter aerogenes (18,2%), kokus Gram negatif (18,2%), Serratia liquefaciens (13,6%), dan Enterobacter cloacae (9,9%). Simpulan: Bakteri aerob di kamar bersalin Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus subtillis. Kata kunci: kamar bersalin, infeksi nosokomial, bakteri aerob

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENJADI SUMBER PENULARAN INFEKSI NOSOKOMIAL DI IRINA A RSUP PROF. DR. R. D. KANDOU MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Yulya Margaretha Longadi ◽  
Olivia Waworuntu ◽  
Standy Soeliongan
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Enterobacter Aerogenes ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Cross Sectional ◽  
Hospital Acquired Infection ◽  
Serratia Liquefaciens ◽  
Two Samples ◽  
Hospital Acquired

Abstract: Nosocomial infection or Hospital Acquired Infection (HAI) is an infection caused by bacteria, parasite, or virus in the hospital, infection occur at least 72 hours since hospitalized. This infection occurs due to lack of hygiene of the environment causing microorganism infection from environment to human, infection can also occur due to transmission of microorganism from one patient to other patients. Inpatients potentially have very high risk of nosocomial infection occur due to continuous requiring treatment for more than 24 hours. Purpose: To determine the existence of aerobic bacteria that could potentially be the source of transmission of nosocomial infection in Irina A RSUP Prof. Dr. R. D. Kandou Manado. Method: This research was descriptive with cross sectional approach. Fourteen samples were taken from the surface of medical equipment, bed, floor, and wall of the treatment room and eight samples were taken from the air. Identification of bacteria was performed by culture on agar medium, staining gram, and biochemical test. Result: Bacillus subtilis found in nine samples (41%), Serratia liquefaciens found in five samples (22,7%), Lactobacillus found in two samples (9,1%), Staphylococcus found in two samples (9,1%), Coccus Gram negative found in two samples (9,1%), Enterobacter aerogenes found in one sample (4,5%), and Enterobacter agglomerans found in one sample (4,5%). Conclusion: Bacillus subtilis is the most bacteria which had been found in this research.Keywords: Aerobic bacteria, Nosocomial infection, Irina A.Abstrak: Infeksi nosokomial atau Hospital Acquired Infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit, atau virus di rumah sakit, infeksi ini timbul sekurang-kurangnya 72 jam sejak masuk rumah sakit. Infeksi ini terjadi akibat kurang bersihnya lingkungan perawatan menyebabkan terjadinya infeksi mikroorganisme dari lingkungan ke manusia, infeksi juga dapat terjadi akibat berpindahnya mikroorganisme dari pasien yang satu ke pasien yang lain. Pasien ruang rawat inap mempunyai risiko yang sangat tinggi mendapatkan infeksi nosokomial, hal ini dikarenakan pasien pada ruang rawat inap memerlukan asuhan, perawatan dan pengobatan secara berkesinambungan lebih dari 24 jam. Tujuan: Untuk mengetahui adanya bakteri aerob yang berpotensi menjadi sumber penularan infeksi nosokomial di IRINA A RSUP Prof. Dr. R. D. kandou Manado. Metode: Metode penelitian yang digunakan adalah deskriptif dengan pendekatan cross sectional study. Sampel diambil dari 14 usapan permukaan peralatan medis, tempat tidur, lantai, dan dinding ruang perawatan dan delapan sampel dari udara ruang. Identifikasi bakteri dilakukan kultur pada media agar, pewarnaan gram, dan uji biokimia. Hasil: Bacillus subtilis ditemukan sebanyak sembilan sampel (41%), Serratia liquefaciens sebanyak lima sampel (22,7%), Lactobacillus sebanyak dua sampel (9,1%), Staphylococcus sebanyak dua sampel (9,1%), Kokus Gram negatif sebanyak dua sampel (9,1%), Enterobacter aerogenes sebanyak satu sampel (4,5%), dan Enterobacter agglomerans sebanyak satu sampel (4,5%). Kesimpulan: Bacillus subtilis merupakan bakteri yang paling banyak ditemukan.Kata Kunci: Bakteri aerob, Infeksi nosokomial, Irina A.

scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Rizka Matoka ◽  
Olivia Waworuntu ◽  
Fredine Rares
Keyword(s):  
Health Care ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Emergency Room ◽  
Enterobacter Aerogenes ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Health Care Associated Infection

Abstract: Nosocomial infection or Health-care Associated Infection (HAIs) according to the WHO in 2011 are infection to patient acquired during a hospital, after ≥48 hours and ≤30 days after out of the hospital. Factor of infection can be differentiated between patients, nurses, doctors, hospital employed, hospital environment and patient visitor or family. The purpose to know the pattern of aerobic bacteria that could potentially to involve nosocomial infection in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado. Type a descriptive observational research with cross sectional study approach. The sampling on the walls, floors, medical equipment, room furniture and the air in the IRDO Prof. Dr. R. D. Kandou Hospital Manado. The results of the 27 samples found bacteria Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%).Conclusion: The most aerobic bacteria in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado is Bacillus sp.(46,55%).Keywords: Emergency Obstetric and Gynecology, nosocomial infection, aerobic bacteria. Abstrak: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) adalah suatu instalasi penanggulangan awal penderita rawat darurat untuk mencegah kematian, dalam menangani kesehatan reproduksi wanita saat tidak hamil ataupun pada saat hamil, persalinan, dan nifas. Infeksi nosokomial atau Health-care Associated Infection (HAIs) menurut WHO 2011 merupakan infeksi yang didapat pasien selama di rumah sakit, setelah ≥ 48 jam dan ≤ 30 hari setelah keluar dari rumah sakit. Faktor terjadinya infeksi bisa dibebakan antar pasien, perawat, dokter, karyawan rumah sakit, lingkungan rumah sakit, dan pengunjung atau keluarga pasien. Tujuan untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian deskriptif observational dengan pendekatan studi cross sectional. Pengambilan sampel pada dinding, lantai, peralatan medis, perabotan ruangan, udara di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 27 sampel ditemukan bakteri Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%). Simpulan: Bakteri aerob di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus sp.(46,55%).Kata kunci: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO), Infeksi nosokomial, bakteri aerob

scholarly journals Isolasi dan identifikasi bakteri aerob yang berpotensi menjadi sumber penularan infeksi nosokomial di ruang ICU Rsad Robert Wolter Mongisidi Teling Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Hilary G. Sarlin ◽  
Heriyannis Homenta ◽  
John Porotu'o
Keyword(s):  
Klebsiella Pneumoniae ◽  
Serratia Marcescens ◽  
Nosocomial Infections ◽  
Enterobacter Cloacae ◽  
Cross Sectional Study ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Serratia Rubidaea

Abstract: Nosocomial infections are infections that arise during the patient was hospitalized and starting to show symptoms for a person being treated or after completion of treatment. Patients in the ICU may have a tendency to nosocomial infections. The purpose of the study is to identify and knowing the most common aerobic bacteria that cause nosocomial infections in the ICU RSAD Robert Wolter Monginsidi Teling Manado.This research is a descriptive cross sectional study. The study samplesare bacteria on the floor, walls, air, bed, and ECG in the ICU RSAD Teling Robert Wolter Monginsidi Manado. The results showed 26 samples out of 26 samples contained bacteria, Bacillus sp. eight samples (30.7%), Enterobacter aerogens six samples (23%), Staphylococcus sp. six samples (23%) and Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. each found as many as one sample (3.8%). Conclusion: The most common bacteria is Bacillus sp.Keywords: nosocomial infection, ICU, aerobic bacteria Abstrak: Infeksi nosokomial adalah Infeksi yang muncul selama penderita tersebut dirawat dirumah sakit dan mulai menunjukkan suatu gejala selama seseorang itu dirawat atau setelah selesai dirawat. Pasien yang berada di ruang ICU dapat mempunyai kecenderungan terkena infeksi nosokomial. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui bakteri aerob yang terbanyak penyebab infeksi nosokomial di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Desain penelitian ini bersifat deskriptif dengan studi cross sectional. Sampel penelitian ialah bakteri pada lantai, dinding, udara, tempat tidur, dan EKG di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Hasil penelitian memperlihatkan dari 26 sampel terdapat 26 sampel yang tumbuh yaitu, Bacillus sp. sebanyak delapan sampel (30,7%), Enterobacter aerogens ditemukan sebanyak enam sampel (23%), Staphylococcus sp. Ditemukan sebanyak enam sampel (23%) sedangkan Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. masing-masing ditemukan sebanyak satu sampel (3,8%). Simpulan: bakteri yang paling banyak ditemukan adalah Bacillus sp. Kata kunci: infeksi nosokomial, ICU, bakteri aerob

scholarly journals POLA BAKTERI PENYEBAB INFEKSI NOSOKOMIAL PADA RUANG PERAWATAN INTENSIF ANAK DI BLU RSUP PROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Anastashia Baharutan ◽  
Fredine E. S. Rares ◽  
Standy Soeliongan
Keyword(s):  
Intensive Care ◽  
Nosocomial Infections ◽  
Enterobacter Aerogenes ◽  
Pediatric Intensive Care ◽  
Enterobacter Cloacae ◽  
Aerobic Bacteria ◽  
Pseudomonas Sp ◽  
Enterobacter Agglomerans ◽  
Gram Negative ◽  
Candida Sp

Abstract: Nosocomial infections is an infection acquired in the hospital in patients treated for at least 72 hours (3x24 hours). Environmental infection caused by bacteria from an existing object within the hospital. Microorganisms are often potentially causing nosocomial infections are Proteus sp., Escherichia colii, Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa. Objective: to know the most aerobic bacteria and the pattern of aerobic bacteria in Pediatric Intensive Care Unit at BLU Dr Prof. Dr R. D. Kandou. Methods: 24 swab samples were taken on the surface of the tool, treatment rooms and 6 samples of air space. Identification of bacterial cultures taken on an agar medium, gram staining and biochemical tests. Results: identified bacteria obtained 11 types of microorganisms, such as Bacillus subtilis, Staphylococcus sp., Enterobacter agglomerans, Serratia rubidaea, Enterobacter aerogenes, Enterobacter cloacae, Coccus Gram-negative, Klebsiella pneumoniae, Candida sp., Pseudomonas sp., Streptococcus sp. Conclusion: Gram negative bacteria are bacteria that are found in the Pediatric Intensive Care Unit. Staphylococcus sp., and Pseudomonas sp., a common bacteria that cause nosocomial infections with Staphylococcus sp., a bacterium that is most found.Keywords: bacteria, germs pattern, nosocomial infections, pediatric intensive care unit.Abstrak: Infeksi nosokomial merupakan infeksi yang didapat di rumah sakit pada pasien yang dirawat paling tidak selama 72 jam (3x24 jam). Infeksi lingkungan yang disebabkan oleh bakteri dari benda yang ada dilingkungan rumah sakit. Mikroorganisme yang sering berpotensi menyebabkan infeksi nosokomial adalah Proteus sp., Escherichia colii, Staphylococcus aureus, Candida albicans, dan Pseudomonas aeruginosa. Tujuan: mengetahui bakteri aerob terbanyak dan pola dari bakteri aerob tersebut pada Ruang Perawatan Intensif anak di BLU RSUP Prof. Dr. R. D. Kandou. Metode: sampel diambil pada 24 usapan permukaan alat, ruangan perawatan dan 6 sampel udara ruang. Identifikasi bakteri dilakukan kultur pada media agar, pewarnaan gram dan uji biokimia. Hasil: bakteri yang teridentifikasi didapatkan 11 jenis mikroorganisme, yaitu Bacillus subtilis, Staphylococcus sp., Enterobacter agglomerans, Serratia rubidaea, Enterobacter aerogenes, Enterobacter cloacae, Coccus Gram negatif , Klebsiella pneumoniae, Candida sp., Pseudomonas sp., Streptococcus sp. Kesimpulan: bakteri gram negatif merupakan bakteri yang banyak ditemukan pada Ruang Perawatan Intensif anak. Staphylococcus sp., dan Pseudomonas sp., merupakan bakteri tersering yang menyebabkan infeksi nosokomial dengan Staphylococcus sp., merupakan bakteri yang terbanyak ditemukan.Kata kunci: bakteri, pola kuman, infeksi nosokomial, ruang perawatan intensif anak.

scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruang neonatal intensive care unit (NICU) RSAD Robert Wolter Mongisidi Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Marshall C. Waworuntu ◽  
Fredine E.S. Rares ◽  
Heriyannis Homenta
Keyword(s):  
Escherichia Coli ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Nosocomial Infections ◽  
Neonatal Intensive Care ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Cross Sectional ◽  
Serratia Liquefaciens

Abstract: Nosocomial infection is an infection which occurs in the hospital caused by microbateria from the hospital. Nosocomial infections that occur in the newborn is a serious problem in every hospital. Neonatal Intensive Care Unit (NICU) is a care unit for newborn who needs special care. Nosocomial infections often occur, especially in infants hospitalized in NICU. The purpose of this research is to determine the pattern of aerobic bacteria that could potentially cause nosocomial infections in NICU RSAD Robert Wolter Monginsidi Manado. The research used descriptive cross sectional method. The amount of the samples are 29 and was taken according to category of the treatment room, room instruments, medical equipments and air. Based on the results, there are 9 species of bacterias included Enterobacter cloacae (27%), Staphylococcus sp (17%), Bacillus subtilis (13%), Klebsiella pneumoniae (13%), Serratia liquefaciens (10%), Serratia marcescens (7%), Enterobacter aerogenes (7%), Neisseria sp (3%) and Escherichia coli (3%). The most bacteria that could potentially cause nosocomial infections were Enterobacter cloacae.Keywords: nosocomial infections, NICU, aerob bacteria Abstrak: Infeksi nosokomial adalah suatu infeksi yang didapat atau terjadi saat pasien dirawat di rumah sakit oleh kuman yang berasal dari rumah sakit. Infeksi nosokomial yang terjadi pada bayi baru lahir merupakan masalah yang serius disetiap rumah sakit. Neonatal Intensive Care Unit (NICU) merupakan unit perawatan untuk bayi baru lahir yang memerlukan perawatan khusus. Infeksi nosokomial sering terjadi terutama pada bayi yang dirawat di NICU. Tujuan penelitian ini yaitu untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan NICU RSAD Robert Wolter Mongisidi Manado. Penelitian ini menggunakan metode deskriptif dengan pendekatan potong lintang (cross sectional). Sampel yang diteliti berjumlah 29 sampel dan di ambil berdasarkan kategori ruang perawatan, perabotan ruangan, peralatan medis dan udara. Berdasarkan hasil penelitian ditemukan 9 spesies bakteri yaitu Enterobacter cloacae (27%), Staphylococcus sp (17%), Bacillus subtilis (13%), Klebsiella pneumoniae (13%), Serratia liquefaciens (10%), Serratia marcescens (7%), Enterobacter aerogenes (7%), Neisseria sp (3%) dan Escherichia coli (3%). Bakteri yang paling banyak ditemukan adalah Enterobacter cloacae. Kata kunci: infeksi nosokomial, NICU, bakteri aerob

scholarly journals Isolasi dan identifikasi bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di Irina F ruangan intermediate care (IMC) Bagian Neurologi RSUP Prof. Dr. R. D. Kandou Manado

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Fransiska E.A. Yobee ◽  
Fredine E.S. Rares ◽  
Heriyannis Homenta
Keyword(s):  
Indoor Air ◽  
Nosocomial Infections ◽  
Healthcare Workers ◽  
Agar Medium ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Aerobic Bacteria ◽  
Intermediate Care ◽  
Biochemical Tests ◽  
Gram Staining

Abstract: Nosocomial infections are infections that occur in the hospital by bacteria originating from the hospital itself This infection can occur within 48 hours after hospitalization, or three days after hospitalization due to the usage of tools, or 30 days after surgery. Nosocomial infections can occur in patients, healthcare workers, and everyone who comes to the hospital. This study was aimed to obtain the microorganisms that could potentially lead to nosocomial infections in the Irina F Intermediate Care (IMC) of Neurology Department at Prof. Dr. R. D. Kandou Hospital Manado. This was a descriptive prospective study. The samples were taken from 14 swabs of furniture at the room, the treatment room, and 8 samples of indoor air. Identification of the bacteria was performed by isolation the bacteria in an agar medium, Gram staining, and biochemical tests. The results showed 5 strains of bacteria, as follows: Enterobacter cloacae (55%), Neisseria sp. (20%), Staphylococcus sp. (15%), Lactobacillius sp. (5%), and Enterobacter aerogenes (5%). Conclusion: Enterobacter cloacae is the most commonly found bacteria in Irina F Intermediate Care (IMC) room of Neurology Department at Prof. Dr. R. D. Kandou Hospital Manado.Keywords: nosocomial infections, patterns of aerobic bacteria Abstrak: Infeksi nosokomial adalah infeksi yang terjadi di rumah sakit oleh kuman yang berasal dari rumah sakit. Infeksi ini dapat terjadi dalam waktu 48 jam setelah rawat inap, atau tiga hari setelah pemakaian alat rumah sakit, atau 30 hari setelah operasi. Infeksi nosokomial dapat terjadi pada penderita, tenaga kesehatan, dan setiap orang yang datang ke rumah sakit. Penelitian ini bertujuan untuk mengetahui mikroorganisme yang berpotensi menyebabkan infeksi nosokomial di Irina F ruangan Intermediate Care (IMC) Bagian Neurologi RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif prospektif. Sampel diambil dari 14 usapan perabotan ruangan dan ruangan perawatan serta 8 sampel dari udara ruangan. Identifikasi bakteri dilakukan melalui isolasi pada media agar, pewarnaan Gram dan uji biokimia. Hasil penelitian memperlihatkan 5 jenis bakteri yang teridentifikasi, yaitu: Enterobacter cloacae (55%), Neisseria sp. (20%), Staphylococcus sp. (15%), Lactobacillius sp. (5%), dan Enterobacter aerogenes (5%). Simpulan: Bakteri Enterobacter Cloacae merupakan bakteri yang paling banyak ditemukan di Irina F ruangan Intermediate Care (IMC) Bagian Neurologi RSUP Prof. Dr. R. D. Kandou Manado Kata kunci: infeksi nosokomial, pola bakteri aerob

scholarly journals POLA BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL PADA KAMAR BERSALIN RSAD ROBERT WOLTER MONGISIDI MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lisa E. Ritto ◽  
Standy Soeliongan ◽  
Fredine E. S. Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Developed Countries ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Isolation And Identification ◽  
Serratia Liquefaciens ◽  
Identification Of Bacteria

Abstract: The situation in countries with limited resources, are radically different in almost every aspect, making pregnant women in developing countries prone to a higher risk of getting nosocomial infections after childbirth than their counterparts in developed countries. One of the most common cause of nosocomial infection is bacterial. The aim of this study was to determine the pattern of aerobic bacteria that could potentially cause nosocomial infection in delivery room RSAD Robert Wolter Monginsidi Manado and followed by isolation and identification of bacteria. This research uses descriptive method with prospective approach. Samples were taken by acquiring swabs from the walls, floor, room furniture, medical equipment, and air as much as 25 samples. As a result, there are Lactobacillus sp. 7 samples (28%), Enterobacter agglomerans 7 samples (28%), Bacillus subtilis 6 samples (24%), Staphylococcus epidermidis 3 samples (12%), Serratia liquefaciens 1 samples (4%) and Enterobacter cloacae 1 samples (4%). This study found the most bacteria are Lactobacillus sp. and Enterobacter agglomerans.Keywords: aerobic bacteria, nosocomial infection, delivery room.Abstrak: Situasi di negara-negara dengan sumber-sumber yang terbatas, secara radikal berbeda hampir dalam setiap aspek membuat perempuan hamil di negara-negara sedang berkembang dihadapkan kepada resiko lebih tinggi untuk mendapatkan infeksi nosokomial sesudah persalinan daripada mitranya di negara-negara maju. Salah satu penyebab tersering infeksi nosokomial adalah bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada kamar bersalin RSAD Robert Wolter Mongisidi Manado yang kemudian dilakukan isolasi dan identifikasi bakteri. Penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan prospektif. Sampel diambil secara usapan dari dinding, lantai, perabotan ruangan, peralatan medis, dan udara sebanyak 25 sampel. Hasil penelitian ditemukan Lactobacillus sp. 7 sampel (28%), Enterobacter agglomerans 7 sampel (28%), Bacillus subtilis 6 sampel (24%), Staphylococcus epidermidis 3 sampel (12%), Serratia liquefaciens 1 sampel (4%) dan Enterobacter cloacae 1 sampel (4%). Kesimpulan dari penelitian ini bakteri terbanyak yang ditemukan adalah Lactobacillus sp. dan Enterobacter agglomerans.Kata kunci: bakteri aerob, infeksi nosokomial, kamar bersalin

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANG RAWAT INAP MATA IRINA F RSUP PROF. DR. R.D. KANDOU MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ameliya S. Japanto ◽  
Standy Soeliongan ◽  
Fredine E. S. Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Agar Medium ◽  
Enterobacter Cloacae ◽  
Hospital Environment ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Identification Of Bacteria

Abstract: Nosocomial infections are infections acquired or occurring while patients were hospitalized. Nosocomial infections can be caused by various agents of disease, like bacteria. The bacteria are found in the hospital environment, including the inpatient unit. Objective: to know the pattern of aerobic bacteria that could potentially cause nosocomial infections in inpatient eyes unit IRINA F Prof. Dr. R. D. Kandou Manado. Methods: 14 swabs were taken at the surface of patient’s beds, treatment rooms and 8 samples of air space. Identification of bacteria carried on an agar medium isolation, Gram staining and biochemical tests. Results: The obtained bacteria identified six types of bacteria, such as Bacillus subtilis, Lactobacillus sp., Coccus negative Gram, Enterobacter agglomerans, Enterobacter cloacae, and Staphylocccus epidermidis. Conclusion: Bacillus subtilis is the bacteria that most commonly found in inpatient eyes unit IRINA FKeywords: nosocomial infections, patterns of aerobic bacteria, inpatient eye unitAbstrak: Infeksi nosokomial adalah infeksi yang didapat atau terjadi saat pasien dirawat di rumah sakit. Infeksi nosokomial dapat disebabkan oleh berbagai agen penyakit, salah satunya ialah bakteri. Bakteri penyebab infeksi sering ditemukan di lingkungan rumah sakit, termasuk di ruang rawat inap. Tujuan : mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruang rawat inap mata IRINA F RSUP Prof. Dr. R. D. Kandou Manado. Metode: sampel diambil pada 14 usapan perabotan ruangan, ruangan perawatan dan 8 sampel udara ruang. Identifikasi bakteri dilakukan isolasi pada media agar, pewarnaan Gram dan uji biokimia. Hasil: bakteri yang teridentifikasi didapatkan enam jenis bakteri, yaitu Bacillus subtilis, Lactobacillus sp., Coccus Gram negatif, Enterobacter agglomerans, Enterobacter cloacae, dan Staphylocccus epidermidis. Kesimpulan: Bakteri Bacillus subtilis merupakan bakteri yang paling banyak ditemukan di ruang rawat inap mata IRINA FKata kunci: infeksi nosokomial, pola bakteri aerob, ruang rawat inap mata

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG DAPAT MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANGAN INSTALASI GIZI BLU RSUP PROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Priska N. Riga ◽  
Velma Buntuan ◽  
Fredine Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Enterobacter Agglomerans ◽  
Vibrio Cholera ◽  
Providencia Rettgeri ◽  
Serratia Liquefaciens ◽  
Providencia Stuartii ◽  
Serratia Rubidaea

Abstract: Kitchen of hospital to be a part or unit of a hospital that is not less important as other services in the hospital , because it provides direct services to patients through the food served by nutritional care . Nosocomial infections are infections that occur during hospitalization where the infection does not exist at the time of admission to the hospital . Infections that occur more than 48 hours after hospital admission . Most infections occur in hospitals is caused by external factors , ie diseases which spread from food and cutlery . Implementation of the food was less qualified health care, in addition to extending the treatment process can also cause cross-infections or nosocomial infections which can be through the cutlery and food . Purpose: To determine the presence of aerobic bacteria that can cause nosocomial infections in the kitchen of BLU Prof. Dr R. D. Kandou Manado .Method: This study was a prospective descriptive study that analyzed 24 samples on cutlery and 6 samples of food in kitchen of BLU Nutrition Dr Prof. Dr R. D. Kandou Manado. Result: From the results of this study were found 11 species of bacteria such as Bacillus subtilis (33,3%), a gram-negative cocci (10%) , Lactobacillus (10%), Enterobacter agglomerans (6,7%), Serratia rubidaea (6,7%), Providencia stuartii (3,3%), Serratia liquefaciens (3,3%), Providencia rettgeri (3,3%), Vibrio cholera (3,3%), Enterobacter cloacae (3,3%), Enterobacter aerogenes (3,3%).Conclusion: The conclusion of this study showed that the most commonly found bacteria was Bacillus subtilis (33,3%).Keywords: nosocomial infection, kitchen of hospital, bacteria.Abstrak: Instalasi gizi menjadi bagian atau unit kerja di rumah sakit yang tidak kalah pentingnya dengan pelayanan lain di rumah sakit, karena memberikan pelayanan langsung kepada pasien melalui makanan yang disajikan oleh asuhan gizi. Infeksi nosokomial adalah infeksi yang terjadi selama perawatan di rumah sakit dimana infeksi tersebut tidak ada pada saat pasien masuk ke rumah sakit. Infeksi yang timbul lebih dari 48 jam setelah masuk rumah sakit. Kebanyakan infeksi yang terjadi di rumah sakit lebih disebabkan oleh faktor eksternal, yaitu penyakit yang penyebarannya ke makanan dan alat makan. Penyelenggaraan makanan yang kurang memenuhi syarat kesehatan, selain memperpanjang proses perawatan juga dapat menyebabkan timbulnya infeksi silang atau infeksi nosokomial yang diantaranya dapat melalui alat makan dan makanan. Tujuan: Untuk mengetahui adanya bakteri aerob yang dapat menyebabkan infeksi nosokomial di ruangan Instalasi Gizi BLU RSUP Prof. Dr. R. D. Kandou Manado. Metode penelitian: Penelitian ini merupakan penelitian deskriptif prospektif yang dianalisis secara desktriptif pada 24 sampel alat makan dan 6 sampel makanan di ruangan Instalasi Gizi BLU RSUP Prof. Dr. R. D. Kandou Manado. Hasil: Dari hasil penelitian ditemukan 11 spesies bakteri yaitu Bacillus subtilis (33,3%), Kokus gram negatif (10%), Lactobacillus (10%), Enterobacter agglomerans (6,7%), Serratia rubidaea (6,7%), Providencia stuartii (3,3%), Serratia liquefaciens (3,3%), Providencia rettgeri (3,3%), Vibrio cholera (3,3%), Enterobacter cloacae (3,3%), Enterobacter aerogenes (3,3%). Kesimpulan: Kesimpulan dari penelitian ini menunjukkan bahwa bakteri yang paling banyak ditemukan dalam penelitian ini adalah Bacillus subtilis (33,3%).Kata Kunci: infeksi nosokomial, instalasi gizi, bakteri.

scholarly journals Biofilm Formation and Quorum-Sensing-Molecule Production by Clinical Isolates of Serratia liquefaciens

2015 ◽  
Vol 81 (10) ◽  
pp. 3306-3315 ◽  
Author(s):  
Sara Remuzgo-Martínez ◽  
María Lázaro-Díez ◽  
Celia Mayer ◽  
Maitane Aranzamendi-Zaldumbide ◽  
Daniel Padilla ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quorum Sensing ◽  
Nosocomial Infections ◽  
Biofilm Formation ◽  
Acyl Chain ◽  
Homoserine Lactone ◽  
Hospital Environment ◽  
Term Survival ◽  
Content Type ◽  
Serratia Liquefaciens

ABSTRACTSerratiaspp. are opportunistic human pathogens responsible for an increasing number of nosocomial infections. However, little is known about the virulence factors and regulatory circuits that may enhance the establishment and long-term survival ofSerratia liquefaciensin the hospital environment. In this study, two reporter strains,Chromobacterium violaceumCV026 and VIR24, and high-resolution triple-quadrupole liquid chromatography–mass spectrometry (LC-MS) were used to detect and to quantifyN-acyl-homoserine lactone (AHL) quorum-sensing signals in 20S. liquefaciensstrains isolated from clinical samples. Only four of the strains produced sufficient amounts of AHLs to activate the sensors. Investigation of two of the positive strains by high-performance liquid chromatography (HPLC)-MS confirmed the presence of significant amounts of short-acyl-chain AHLs (N-butyryl-l-homoserine lactone [C4-HSL] andN-hexanoyl-l-homoserine lactone [C6-HSL]) in both strains, which exhibited a complex and strain-specific signal profile that included minor amounts of other short-acyl-chain AHLs (N-octanoyl-l-homoserine lactone [C8-HSL] andN-3-oxohexanoyl-l-homoserine lactone [OC6-HSL]) and long-acyl-chain (C10, C12, and C14) AHLs. No correlation between biofilm formation and the production of large amounts of AHLs could be established. Fimbria-like structures were observed by transmission electron microscopy, and the presence of the type 1 fimbrial adhesin genefimHin all strains was confirmed by PCR. The ability ofS. liquefaciensto adhere to abiotic surfaces and to form biofilms likely contributes to its persistence in the hospital environment, increasing the probability of causing nosocomial infections. Therefore, a better understanding of the adherence properties of this species will provide greater insights into the diseases it causes.

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