scholarly journals LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

2021 ◽  
Author(s):  
Zeynep AKAR ◽  
Çağlar DEMİR ◽  
Ozan ALKAN ◽  
Zehra CAN ◽  
Bülent AKAR
Keyword(s):  
Antioxidant Activities ◽  
Rp Hplc ◽  
Arum Italicum

Isolation and Structural Characterization of Antioxidant Peptides from Degreased Apricot Seed Kernels

2018 ◽  
Vol 101 (5) ◽  
pp. 1661-1663 ◽  
Author(s):  
Haisheng Zhang ◽  
Jing Xue ◽  
Huanxia Zhao ◽  
Xinshuai Zhao ◽  
Huanhuan Xue ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antioxidant Activities ◽  
Gel Filtration ◽  
Reversed Phase ◽  
Amino Acid Sequences ◽  
Seed Kernel ◽  
Antioxidant Peptides ◽  
Food Preservatives ◽  
Isolation And Purification ◽  
Rp Hplc

Abstract Background: The composition and sequence of amino acids have a prominent influence on the antioxidant activities of peptides. Objective: A series of isolation and purification experiments was conducted to explore the amino acid sequence of antioxidant peptides, which led to its antioxidation causes. Methods: The degreased apricot seed kernels were hydrolyzed by compound proteases of alkaline protease and flavor protease (3:2, u/u) to prepare apricot seed kernel hydrolysates (ASKH). ASKH were separated into ASKH-A and ASKH-B by dialysis bag. ASKH-B (MW < 3.5 kDa) was further separated into fractions by Sephadex G-25 and G-15 gel-filtration chromatography. Reversed-phase HPLC (RP-HPLC) was performed to separate fraction B4b into two antioxidant peptides (peptide B4b-4 and B4b-6). Results: The amino acid sequences were Val-Leu-Tyr-Ile-Trp and Ser-Val-Pro-Tyr-Glu, respectively. Conclusions: The results suggested that ASKH antioxidant peptides may have potential utility as healthy ingredients and as food preservatives due to their antioxidant activity. Highlights: Materials with regional characteristics were selected to explore, and hydrolysates were identified by RP-HPLC and matrix-assisted laser desorption ionization-time-of-flight-MS to obtain amino acid sequences.

scholarly journals Methylated Flavonols from Amomum koenigii J.F.Gmel. and Their Antimicrobial and Antioxidant Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Minh Giang Phan ◽  
Thi Viet Huong Do ◽  
Quoc Binh Nguyen
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Spectroscopic Techniques ◽  
Special Group ◽  
X Ray ◽  
Rp Hplc ◽  
Dpph Scavenging ◽  
First Time ◽  
Antimicrobial Assay

Methylated flavonols form a special group with modulating biological activities in comparison with kaempferol and quercetin. The present study isolated ten compounds including two kaempferol methyl ethers: 5-hydroxy-3,7,4′-trimethoxyflavone (1), 3-hydroxy-5,7,4′-trimethoxyflavone (6); four quercetin methyl ethers: retusin (5-hydroxy-3,7,3′,4′-tetramethoxyflavone) (4), 3,5-dihydroxy-7,3′,4′-trimethoxyflavone (5), 3,4′-dihydroxy-5,7,3′-trimethoxyflavone (7), and 3,5,7,3′,4′-pentamethoxyflavone (9); β-sitosterol (2); 5-hydroxy-1-(4′-hydroxyphenyl)eicosan-3-one (3); p-hydroquinone (8); and vanillic acid (10) from the rhizomes and fruit of Amomum koenigii J.F.Gmel. (Zingiberaceae). Their structures were determined by MS, NMR, and X-ray spectroscopic techniques. Among the methylated flavonols, 1, 4–7, and 9 were isolated for the first time from the rhizomes, while 1, 4, and 5 were isolated from the fruit. Compounds 2, 3, 7, 8, and 10 were reported for the first time from the species. Three main methylated flavonols 1, 4, and 5 were quantitatively analyzed in the rhizomes of A. koenigii by RP-HPLC-DAD; their contents were determined to be 1.81% (1), 1.38% (4), and 1.76% (5). The antimicrobial assay against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Aspergillus niger, Fusarium oxysporum, Candida albicans, and Saccharomyces cerevisiae and antioxidant DPPH scavenging test were performed for the isolated methylated flavonols.

Extraction, quantification and antioxidant activities of flavonoids, polyphenols and pinitol from wild and cultivated Saraca asoca bark using RP-HPLC-PDA-RI method

2017 ◽  
Vol 103 ◽  
pp. 73-80 ◽  
Author(s):  
Rashi Tewari ◽  
Madhuri Gupta ◽  
Furkan Ahmad ◽  
Prasant Kumar Rout ◽  
Laxminarain Misra ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Saraca Asoca ◽  
Rp Hplc

scholarly journals Dromedary Milk Protein Hydrolysates Show Enhanced Antioxidant and Functional Properties

2020 ◽  
Vol 58 (2) ◽  
pp. 147-158
Author(s):  
Olfa Oussaief ◽  
Zeineb Jrad ◽  
Isabelle Adt ◽  
Touhami Khorchani ◽  
Halima El-Hatmi
Keyword(s):  
Molecular Mass ◽  
Functional Properties ◽  
Milk Protein ◽  
Molecular Mass Distribution ◽  
Antioxidant Activities ◽  
Milk Proteins ◽  
Protein Hydrolysates ◽  
Rp Hplc ◽  
Dromedary Milk ◽  
Hydrolysis Of

Research background. Milk protein hydrolysates have received particular attention due to their health-promoting effects. Dromedary milk differs from the milk of other dairy animals in the composition and structure of its protein components, which give it unique properties. The bioactivity and functionality of whole dromedary milk proteins and their enzymatic hydrolysates have not received much attention, hence this study aims to investigate the effect of enzymatic hydrolysis of dromedary milk proteins on their antioxidant activities and functional properties. Experimental approach. Dromedary milk proteins were treated using four proteolytic enzymes (pepsin, trypsin, α-chymotrypsin and papain) and two mixtures of enzymes (pancreatin and pronase). The degree of hydrolysis was measured to verify the hydrolysis of the proteins. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography served to determine the molecular mass distribution of the hydrolysates while reversed phase-high performance liquid chromatography (RP-HPLC) was conducted to explore their hydrophobicity. The antioxidant activities were evaluated using various in vitro tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacities, iron(III) reducing ability and chelating activity. Besides, functional properties such as solubility, foaming and emulsification were assessed. Results and conclusions. Dromedary milk protein hydrolysates exhibited different degrees of hydrolysis ranging from 17.69 to 41.86 %. Apart from that, the hydrolysates showed different electrophoretic patterns, molecular mass distribution and RP-HPLC profiles demonstrating the heterogeneity of the resulting peptides in terms of molecular mass and polarity. The hydrolysates displayed significantly higher antioxidant capacities than the undigested proteins at all the tested concentrations. Iron(II) chelating activity was the most improved assay after proteolysis and the hydrolysate generated with pancreatin had the highest chelating power. Dromedary milk protein hydrolysates possessed good solubility (>89 %). Further, foaming and emulsifying properties of dromedary milk proteins were enhanced after their proteolysis. These interfacial properties were influenced by the enzymes employed during proteolysis. Novelty and scientific contribution. Enzymatic hydrolysis of dromedary milk proteins is an effective tool to obtain protein hydrolysates with great antioxidant and functional properties. These results suggest that dromedary milk protein hydrolysates could be used as a natural source of antioxidant peptides to formulate functional foods and nutraceuticals.

scholarly journals Proximate composition, antioxidant activities and phenolic composition of Cucumis sativus forma hardwickii (Royle) W. J. de Wilde & Duyfjes

2017 ◽  
Vol 9 (1) ◽  
pp. 101 ◽  
Author(s):  
Usmangani A Attar ◽  
Savaliram G Ghane
Keyword(s):  
Cucumis Sativus ◽  
Functional Food ◽  
Antioxidant Activities ◽  
Antinutritional Factors ◽  
Antioxidant Potential ◽  
Hydroxybenzoic Acid ◽  
Radical Scavengers ◽  
Rp Hplc ◽  
Bioactive Properties ◽  
First Time

In the present investigation, nutritional, anti-nutritional and antioxidant potential of Cucumis sativus forma hardwickii was evaluated for the first time. The results revealed that leaf and fruit of Cucumis sativus f. hardwickii were rich source of ash, carbohydrate, fat, fibre and proteins. In addition, appreciable amounts of Vitamin C and E were also observed. Considerable antinutritional factors in leaf and fruit were noted which can be reduced by different processing methods. In sequential solvent extraction, methanol found to be the best in the extraction of antioxidants, which was supported with the highest antioxidant activities. Among all ¬¬the extracts, it was noted that leaves extracted with methanol had higher amount of phenols and flavonoids than fruit. In addition, the potent radical scavengers mainly phenolics were detected using RP-HPLC where tannic acid, gallic acid and hydroxybenzoic acid were also quantified in leaf and fruit. Superior antioxidant potential may be due to the presence of other bioactive compounds present in fruit. Additional research on bioactive properties of this plant is needed to explore this neglected wild cucumber for nutraceutical purpose and as a functional food.

Structural characterization, antioxidant, and antibiofilm activities of Coffea canephora green seeds

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohammed Aissaoui ◽  
Mohammed Nadjib Rahmoun ◽  
Hassan Latrache ◽  
Said Barek ◽  
Ayoub Elassri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Adhesion ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Coffea Canephora ◽  
Coumaric Acid ◽  
New Approach ◽  
Butanol Extract ◽  
Microdilution Method ◽  
Rp Hplc

AbstractObjectivesIn order to explore Coffea canephora green seeds as natural extract for application in the functional-food industry, we focused this study to the evaluation of the antioxidant and the antiadhesion effect of C. canephora green seeds extracts.MethodsThe analysis of C. canephora green seeds extracts was carried out by RP-HPLC-PDA. These extracts were screened for antioxidant activities by ABTS and phenanthroline assays. The antibacterial activity was determined by microdilution method against three reference bacteria. The inhibition of bacterial adhesion at 1/8 MIC was carried out against three reference bacteria.ResultsThe RP-HPLC-PDA revealed the presence of gallic acid, vanillin, quercetin, chlorogenic acid, and P-coumaric acid. The n-buatnol extract have the highest activity ABTS assays (3.96 ± 0.08 μg/mL). For this extract, the A0.5 was 1.90 ± 0.05 μg/mL for phenanthroline assay. The n-butanol extract and the methanolic extract have the higher antibacterial activity against Staphylococcus aureus ATCC 25923 (40 µg/mL).At MIC/8, the extracts of C. canephora showed 70% higher antidhesive activity against S. aureus ATCC 25923.ConclusionsOur finding provides an effective and specific new approach to the search of antioxidant and antiadhesive compounds for different uses.

Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

2017 ◽  
Vol 87 (3-4) ◽  
pp. 191-200 ◽  
Author(s):  
Nidhal Soualeh ◽  
Aliçia Stiévenard ◽  
Elie Baudelaire ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Good Alternative ◽  
Activity Levels ◽  
Salix Alba ◽  
Rosa Canina ◽  
Free Process ◽  
Plant Powders ◽  
Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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