Antioxidant activities and RP-HPLC identification of polyphenols in the ethyl acetate extract of Tunisian Juglans regia L treated barks.

2012 ◽  
Vol 6 (8) ◽  
Author(s):  
Emira Noumi
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Juglans Regia ◽  
Rp Hplc

ANTIOXIDANT ACTIVITIES OF EXTRACTS FROM THE LEAVES AND STEM BARKS OF Artocarpus scortechinii KING

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Shajarahtunnur Jamil ◽  
Norazah Basar ◽  
Norzafneza Mohd Arriffin
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Calibration Graph ◽  
Total Phenolic ◽  
Scavenging Capacity

The antioxidant activities of extracts (n-hexane, dichloromethane, ethyl acetate and methanol) from the leaves and stem barks of Artocarpus scortechinii were evaluated using various biochemical assays. The quantification of the Total Antioxidant Capacity was measured using ferric reducing antioxidant potential (FRAP) and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) assays. While, the qualitative of The Total Phenolic Content (TPC) was determined via standard gallic acid calibration graph which was expressed as mg gallic acid equivalent (GAE)/g of dry weight (dw) using Folin Ciocalteau’s reagent. Among all the extracts tested, the methanolic extract of the stem barks showed the highest phenolic content with TPC value of 136.84 mg GAE/g dry weight (dw). FRAP results were expressed as mM equivalent to FeSO4.7H2O by calculating from the standard FeSO4.7H2O calibration graph. The ethyl acetate extract of the stem barks showed the most significant reducing potential in the range between 0.27-2.47 mM FRAP. ABTS+˙ radical scavenging capacity showed that the ethyl acetate extract of the stem barks had the highest scavenging capacity at concentration 1.0 mM with percentage of 90.9%.

scholarly journals Phenolic content and antioxidant activity of Phymatopteris hastata

2011 ◽  
Vol 76 (11) ◽  
pp. 1485-1496 ◽  
Author(s):  
Wei Su ◽  
Peiyuan Li ◽  
Lini Huo ◽  
Caiying Wu ◽  
Nana Guo ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Alcohol Extract

Various solvent extracts of Phymatopteris hastata, a traditional Chinese medicinal material, were screened for their antioxidant activities. Four systems of in vitro testing were employed to investigate the antiradical and antioxidant effect, i.e., the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) systems, the hydroxyl radical scavenging assay and the reducing power. In addition, butylated hydroxytoluene (BHT), a widely used synthetic antioxidant, was also studied for comparison. The results revealed that the ethyl acetate extract exhibited outstanding antioxidant activities, which was close or even superior to BHT. Furthermore, the total phenolic (TP) and total flavonoid (TF) contents of different extracts were measured, expressed as gallic acid and rutin equivalent, respectively. The antioxidant activities and the TP/TF content of different extracts followed the same order: ethyl acetate extract > butyl alcohol extract > petroleum ether extract, showing a good correlation between the antioxidant activities and the TP/TF content. The results showed that these extracts, especially the ethyl acetate extract, were rich in phenolics and flavonoids and could be considered as natural antioxidants.

scholarly journals Antioxidant and Protective Effect of Ethyl Acetate Extract ofPodophyllum hexandrumRhizome on Carbon Tetrachloride Induced Rat Liver Injury

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Showkat Ahmad Ganie ◽  
Ehtishamul Haq ◽  
Akbar Masood ◽  
Abid Hamid ◽  
Mohmmad Afzal Zargar
Keyword(s):  
Carbon Tetrachloride ◽  
Protective Effect ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Peroxide Radical ◽  
Scavenging Activity

The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, superoxide radical scavenging activity, hydrogen peroxide radical scavenging activity, and its reducing power ability. All thesein vitroantioxidant activities were concentration dependent, which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential ofPodophyllum hexandrumextract was also evaluated in male Wistar rats against carbon tetrachloride- (CCl4-) induced liver damage. Pretreated rats were given ethyl acetate extract at 20, 30, and 50 mg/kg dose prior to CCl4administration (1 mL/kg, 1:1 in olive oil). Rats pretreated withP. hexandrumextract remarkably prevented the elevation of serum AST, ALT, LDH, and liver lipid peroxides in CCl4-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extract in all the experimental groups. The extract at the tested doses also restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase, and glutathione-S-transferase) significantly. This study suggests that ethyl acetate extract ofP. hexandrumhas a liver-protective effect against CCl4-induced hepatotoxicity and possessin vitroantioxidant activities.

Antioxidant Activities of Mirabilis jalapa L. Seed Epicarp Extract

2012 ◽  
Vol 550-553 ◽  
pp. 1768-1772
Author(s):  
Xin Hong Wang ◽  
Jing Tao Dai
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mirabilis Jalapa

Mirabilis jalapa L. (Nyctaginaceae) is a traditional specie and widely distributed in most parts of China. Its leaves, roots, seed and flowers are used as drugs pharmacological activity. This is the first report on the biological activity against activities of scavenging free radical and ferric reducing antioxidant power (FRAP). Mirabilis jalapa L. seed epicarp (MJSE) was immersed with ethanol (or methanol, ethyl acetate, water) in the ultrasonic instrument, then determination of total phenolic content (TPC) and total flavonoid content (TFC) of these extracts were performed. Furthermore, antioxidant activities of these extracts were evaluated by using DPPH radical scavenging, hydroxyl radical scavenging and FRAP. The TPC, TFC, FRAP and scavenging activities against DPPH and OH radical were in the following order ethyl acetate >ethanol>methanol>water. The TPC of ethyl acetate extract is 4.24 mg GAE /g of dry power weight (DPW), and TFC 0.39 mg RE/ g DPW. ethyl acetate extract exhibit high free radical scavenging rate, IC50 of DPPH and OH assay were 6.62 mg DPW/ml and 3.49 mg DPW/ml, respectively. The TPC values, IC50 obtained from DPPH assay and FRAP assay were highly correlated with correlation of determination (R2 = 0.9878, R2 = 0.9419). The present study confirmed that MJSE has a strong antioxidant activity and can be considered to be safe and an inexpensive source of natural antioxidants and drugs.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

scholarly journals Antibacterial and Antioxidant Activities of Leaf Extracts from Juglans sinensis, and its Phenolic Compositions

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Kook-Il Han ◽  
Eui-Gil Jung ◽  
Bharat Bhusan Patnaik ◽  
Chang-Il Hong ◽  
Young-Jun Kim ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Farming Practices ◽  
Leaf Extracts ◽  
Concentration Method

The beneficial properties of walnut depend on climate, genotype, and farming practices. We investigated the antibacterial and antioxidant activities of Juglans sinensis Dode cultivars grown in South Korea. Antibacterial activities were assessed using the minimum inhibitory concentration method and antioxidant activities using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Ethyl acetate and chloroform extracts of J. sinensis leaves showed the highest antibacterial activity against Staphylococcus aureus SG511, Klebsiella aerogenes 1522E, and/or Enterobacter cloacae P99. DPPH and ABTS radical scavenging activities were found to be highest in the ethyl acetate extract. The total flavonoid and polyphenolic contents in the extract were 219.27 mg/g quercetin equivalents and 245.11 mg/g of gallic acid equivalents, respectively. Phenolic acids (gallic and vanillic) and flavonoids (catechin, rutin, and juglone) were detected by high performance liquid chromatography. We conclude that J. sinensis cultivars provide accessible sources of natural antibacterials and antioxidants.

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF A SPECIES OF ASPERGILLUS: AN ENDOPHYTIC FUNGUS OF SCHIMA WALLICHII (DC.) KORTH. LEAVES

2017 ◽  
Vol 10 (9) ◽  
pp. 361
Author(s):  
Sunrit Basu Sarbadhikary ◽  
Narayan Chandra Mandal
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Mode Of Action ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Pathogenic Fungi ◽  
Structural Features ◽  
Fungal Endophyte ◽  
Schima Wallichii

  Objective: The main goals of this study were to check the antimicrobial and antioxidant potentials of an endophytic fungal strain isolated from the leaves of Schima wallichii (DC.) Korth.Methods: The antibacterial and antifungal activities of the isolated fungal endophyte Visva-Bharati endophyte fungal (VBEF2) were checked by disc diffusion and agar well diffusion methods, respectively, against six pathogenic bacteria and four pathogenic fungi. The minimum inhibitory concentration (MIC) values and the mode of action of VBEF2 against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were determined following colony-forming units (CFU) counting method. Antioxidant activity of the isolate was studied following 2, 2-diphenyl-2- picrilhydrazyl (DPPH) reduction assay.Results: The cell-free supernatants (CFS) of VBEF2 exhibited excellent antibacterial activity against all the bacteria used. The ethyl acetate extract of the endophyte was found to have the MIC of 50 μg/ml and 150 μg/ml against S. aureus and E. coli, respectively. It showed bactericidal mode of action against both of them. The CFS of the strain VBEF2 also showed excellent activities against two animal and two plant pathogenic fungi by producing zones of inhibition in the range of 10-20 mm. In the DPPH scavenging antioxidant assay, the ethyl acetate extract of VBEF2 was found with a low IC50 value of 19.01 μg/ml. The strain VBEF2 was identified as a species of Aspergillus based on its colony morphology and structural features observed under a compound light microscope.Conclusion: The strain VBEF2 can be implemented in various fields of pharmaceutical industry as it showed multidimensional beneficial attributes such as excellent antimicrobial and antioxidant activity.

scholarly journals Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in Lavandulapubescens

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1027
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Ye Eun Park ◽  
Ye Jin Kim ◽  
Chanung Park ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Information ◽  
Integrated Analysis ◽  
Cdna Clones ◽  
Dependent Manner ◽  
Strong Activity

Lavandula pubescens, belonging to the Labiatae family, is a newly discovered strongly aromatic species of lavender that is potentially beneficial for human health. Given the economic importance of lavender species, we sought in this study to characterize the terpenoid biosynthesis of L. pubescens by obtaining transcriptomic and metabolic datasets. Transcriptome analysis of L. pubescens grown aseptically in tissue culture medium yielded 124,233 unigenes with an average length of 470 bp and N50 value of 522 bp from 9,476,122,928 raw reads. In order to provide relevant biological information, the unigenes were annotated using the following public databases: National Center for Biotechnology Information (NCBI) nucleotide (NT) and non-redundant protein (NR), Brassica (BRAD), Arabidopsis Information Resource (TAIR), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). NR annotation results revealed that L. pubescens is genetically closely related to Sesamum indicum. On the basis of the transcriptome data, a total of 14 cDNA clones encoding the terpene biosynthetic genes LpDXS, LpMCT, LpMCS, LpHDR, LpIDI, LpAACT, LpHMGS, LpHMGR, LpMVK, LpPMK, LpMVD, LpGPPS, LpSQS, and LpGGPPS were identified in L. pubescens. These were quantified in the roots, stems, and leaves of L. pubescens using quantitative real-time polymerase chain reaction (qRT-PCR), which revealed that the gene expression levels were higher in the leaves and stems than in the roots, which was found to be consistent with the levels of ursolic and oleanolic acids in the different organs using high-performance liquid chromatography (HPLC). A total of 48 hydrophilic metabolites were identified and quantified in the organs using gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Furthermore, the antioxidant activity of an ethyl acetate extract of L. pubescens leaves was examined using different methods to determine the potential therapeutic properties. A reducing power assay revealed that the absorbance values increased in a concentration-dependent manner, whereas a 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay indicated the strong activity (60.4 ± 0.9%) of the ethyl acetate extract at a concentration of 100 µg/mL, which also showed strong hydrogen peroxide (57.4 ± 2.7%), superoxide radical (62.1 ± 0.7%), and hydroxyl radical (58.6 ± 0.4%) scavenging activities.

scholarly journals Antimicrobial and Antioxidant Activities of Streptomyces sps Isolated from Muthupettai Mangrove Soil

2021 ◽  
pp. 210-234
Author(s):  
C. Nivetha ◽  
T. Deepika ◽  
A. Arjunan ◽  
P. Sivalingam ◽  
N. Revathi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Rrna Gene ◽  
Mangrove Soil ◽  
Soil Sediment

Muthupet mangrove forest soil sediment was the abundant resource of the actinomycetes with distinct nature of bioactive compounds. The soil sediment was collected at 1-3meter away from bank. The present study was focused on isolation, identification and antimicrobial activity of the actinomycetes from Muthupet mangrove soil samples. Totally 32 actinomycetes strains was isolated and screened for antimicrobial activity against bacterial and fungal pathogens. Among 32 isolates 16 have antibacterial activity and 10 have antifungal activity but MG-3 and MG-4 showed maximum activity against both all the test pathogens. These two strains are gram-positive, rod-shaped, MG-3 possessing an earthy characteristic odour and MG-4 produce purple color pigment. The isolates were confirmed as Streptomyces sp. based on morphological, cultural, biochemical and physiological observations, as well as identification using the 16S rRNA gene sequence, it showed 98% similarity with Streptomyces parvus for MG-3 and Streptomyces californicus for MG-4. Bioactive compounds were extracted from Streptomyces using different solvents such as ethyl acetate, methanol, chloroform, hexane and antibacterial activities were assayed against test pathogens, ethyl acetate extract showed maximum zone of inhibition when compared with other solvents. The Minimum inhibitory concentration of ethyl acetate extract was found ranged between 1.96-3.9 μg/ml. The invitro antioxidant capacity of the crude extract was estimated by DPPH, ferric reducing power assay, H202 radical scavenging assay, phosphomolybdenum assay and total antioxidant activities. The characterization of crude extracts was analyzed by FTIR and GC-MS. From the results, it is clear that the ethyl acetate crude extract of S.parvus MG-3 and S.californicus MG-4 possesses high antimicrobial and antioxidant activity and suggested that the isolated strains could be a potential for the nature resource of pharmaceutical.

scholarly journals NEPHROPROTECTIVE ACTIVITY OF THE ETHNOMEDICINAL PLANTS CAESALPINIA BONDUC AND MOMORDICA DIOICA FROM NE INDIA AGAINST CISPLATIN INDUCED CHEMOTHERAPEUTIC TOXICITY

2018 ◽  
Vol 10 (6) ◽  
pp. 96
Author(s):  
Anindita Talukdar ◽  
Ranjan Dutta Kalita ◽  
Nilakshi Gohain ◽  
Kandarpa Kr. Saikia ◽  
Mohan Chandra Kalita
Keyword(s):  
Ethyl Acetate ◽  
Plant Extracts ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Control Group ◽  
Nephroprotective Activity

Objective: Cisplatin a potent anticancer drug is used for treatment against different forms of cancer. However a major limitation of cisplatin is its nephrotoxicity and severe side effects. The main objective of the present study was to investigate the nephroprotective activity of the medicinal plants Caesalpinia bonduc and Momordica dioica against cisplatin-induced nephrotoxicity. Methods: In the present study wistar rat models were intraperitoneally injected with cisplatin for eight consecutive days Plant extracts of different compositions were administered for eight days after injection of cisplatin. The polyphenolic content of the crude extracts was investigated using the Folin Ciocalteau method while the flavonoid estimation was carried out using the aluminium chloride method. The antioxidant activities of the extracts were assayed using the DPPH radical scavenging method. Plant extracts were tested for their nephroprotective ability using modified Berthelot method, uricase method, alkaline picrate method.Results: In the present study it was found that the level of creatinine, urea, uric acid and blood urea nitrogen was lowered by the crude hexane extract of Momordica dioica and ethyl acetate extract of Caesalpinia bonduc as compared to the control group. The total phenolic content and the total flavonoid content was found to be highest in the hexane extract of Momordica dioica with 110.51±1.0 μg/GAE and 1.92±0.15 μg/QE. The methanol extract of Caesalpinia bonduc was found to be the highest in inhibition of the DPPH free radicals with nearly 80% inhibition.Conclusion: The present study revealed the nephroprotective ability of the hexane extract of Momordica dioica and ethyl acetate extract Caeselpinia bonduc. The extracts were also found to have higher antioxidant activity and therefore their nephroprotective activity might be due to attenuation of oxidative stress.

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