scholarly journals Antibacterial and Antifungal Activity of Novel Freshwater Bacterium Tabrizicola aquatica as a Prominent Natural Antibiotic Available in Qurugol Lake

2020 ◽  
Vol 26 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Vahideh Tarhriz ◽  
Shirin Eyvazi ◽  
Elia Shakeri ◽  
Mohammad Saeid Hejazi ◽  
Azita Dilmaghani
Keyword(s):  
Neurospora Crassa ◽  
Pseudomonas Syringae ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Growth Zone ◽  
Antagonistic Effect ◽  
Klebsiella Pneumonia ◽  
Tabrizicola Aquatica ◽  
Promising Source

Background : Recently, resistant pathogenic microorganisms have become increasingly wide spread. The search for new natural antibiotics is a viable solution to this problem. For this aim we investigated the antimicrobial ability of Tabrizicola aquatica, the novel bacterium isolated from Qurugol Lake located nearby Tabriz city, Iran. Methods: The antimicrobial properties of Tabrizacola aquatica was investigated using well diffusion test. Tabtizicola aquatica was incubated at 40℃ in shaking incubator at 150 rpm for 14 days. The culture was centrifuged to obtain cell free supernatant, which was sterilized using 0.2 μm filter paper and lyophilized. Microorganisms were lawn and then wells were prepared over the agar plates. About 100 ml of the diluted lyophilized supernatant was added to the wells. The plates then were incubated at 37℃. After 48 hours, antimicrobial activity was defined by measuring the inhibition zone diameter. Results: The bacterial filtrates had considerable antagonistic effect against Escherichia coli, Rhizobium radiobacter, Pseudomonas syringae, Erwinia amylovora, Botrytis cinerea, Neurospora crassa and Fusarium oxysporum. However, the filtrates did not show any inhibitory action on the Aspergillus flavus and Klebsiella pneumonia. The supernatant decreased the growth zone on Streptococcus aureus, Pseudomonas aeruginosa, Shigella flexneri, Xanthomonas camoestris and Bassilus cereos. The result of MIC against pathogens was found for Neurospora crassa in the 50 µg/mL. Conclusion: The results, suggested that Tabrizicola aquatica and similar bacteria can be helpful to control freshwater natural water sources from pathogenic microorganism. Moreover, microbial natural products are still the most promising source of new antibiotics. Our results point out a scope for characterization of the metabolites and could be a candidate in the identification of novel antibiotics.

scholarly journals Polydopamine-Inspired Surface Modification of Polypropylene Hernia Mesh Devices via Cold Oxygen Plasma: Antibacterial and Drug Release Properties

Coatings ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 164 ◽  
Author(s):  
Xiakeer Saitaer ◽  
Noor Sanbhal ◽  
Yansha Qiao ◽  
Yan Li ◽  
Jing Gao ◽  
...  
Keyword(s):  
Drug Release ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Antimicrobial Property ◽  
Antimicrobial Properties ◽  
Major Complication ◽  
Inhibition Zone ◽  
Repair Process ◽  
Hernia Surgery ◽  
Maximum Inhibition

Mesh infection is a major complication of hernia surgery after polypropylene (PP) mesh implantation. Modifying the PP mesh with antibacterial drugs is an effective way to reduce the chance of infection, but the hydrophobic characteristic of PP fibers has obstructed the drug adhesion. Therefore, to prepare antimicrobial PP mesh with a stable drug coating layer and to slow the drug release property during the hernia repair process has a great practical meaning. In this work, PP meshes were coated by bio-inspired polydopamine (PDA), which can load and release levofloxacin. PP meshes were activated with cold oxygen plasma and then plasma activated PP fibers were coated with PDA. The PDA coated meshes were further soaked in levofloxacin. The levofloxacin loaded PP meshes demonstrate excellent antimicrobial properties for 6 days and the drug release has lasted for at least 24 h. Moreover, a control PP mesh sample without plasma treatment was also prepared, after coating with PDA and loading levofloxacin. The antimicrobial property was sustained only for two days. The maximum inhibition zone of PDA coated meshes with and without plasma treatment was 12.5 and 9 mm, respectively. On all accounts, the modification strategy can facilely lead to long-term property of infection prevention.

PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL PROPERTIES OF DIOSCOREA BULBIFERA TUBER

2017 ◽  
Vol 10 (12) ◽  
pp. 317
Author(s):  
Dahiya P
Keyword(s):  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Investigation ◽  
Dioscorea Bulbifera ◽  
Acinetobacter Sp ◽  
Almost All

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals PG and Research Department of Zoology, Kongunadu Arts and Science College, Coimbatore

2015 ◽  
Vol 2 (2) ◽  
pp. 139-142
Author(s):  
Lekeshmanaswamy M ◽  
Anusiya devi K ◽  
Manish Kumar B ◽  
Vasuki C.A
Keyword(s):  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Maximum Activity ◽  
Klebsiella Pneumonia ◽  
Moderate Activity ◽  
Candida Sp ◽  
Antibacterial Assay ◽  
Padina Gymnospora ◽  
Science College

In this study Gracilaria edilis, sargassam and Padina gymnospora from Raameshwaram sea, Tamil Nadu were collected, identified and tested against various pathogenic bacteria. In Antibacterial assay Acetone shows the maximum number of activity against the Salmonella typi (32mm) length of inhibtions occurred andAcetone shows the minimum activity against Klebsiella pneumonia (18mm) of the inhibition level. Under the Antifungal assay Acetone shows the maximum activity against the penicillium.sp, (10mm) of the inhibition. Acetone shows the moderate activity against the Aspergillus niger (5mm) of inhibition and the minimumactivity in candida sp,. (8mm) of inhibition zone level.

scholarly journals Thymol Chemotype Origanum vulgare L. Essential Oil as a Potential Selective Bio-Based Herbicide on Monocot Plant Species

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 595 ◽  
Author(s):  
Daniela Gruľová ◽  
Lucia Caputo ◽  
Hazem S. Elshafie ◽  
Beáta Baranová ◽  
Laura De Martino ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Plant Species ◽  
Pseudomonas Syringae ◽  
Penicillium Expansum ◽  
Chemical Pollution ◽  
Origanum Vulgare ◽  
Promising Source ◽  
Dicot Species

Searching for new bio-based herbicides is crucial for decreasing chemical pollution, protecting the environment, and sustaining biodiversity. Origanum vulgare is considered a promising source of essential oil with herbicidal effect. The mode of action is not known. The present study focused on (1) comparison of phytotoxic activity of Origanum vulgare EO on monocot (Triticum aestivum and Hordeum vulgare) and dicot species (Lepidium sativum and Sinapis alba); (2) and evaluating other antimicrobial biological activities against phytopatogen bacteria (Clavibacter michiganensis, Pseudomonas syringae pv. phaseolicola, Pseudomonas savastanoi, and Xanthomonas campestris); antifungal activity against Monilinia fructicola, Aspergillus niger, Penicillium expansum, and Botrytis cinerea; cytotoxic activity and antioxidant activity. According to the GC/MS analyses, the EO belongs to the thymol chemotype O. vulgare with its high content of thymol (76%). Germination of all four species was not influenced by EO. The phytotoxic effect was statistically significant in the monocot species, while in the dicot species the opposite was observed—a stimulation effect, which was also statistically significant. Strong biological activity of O. vulgare EO was noted on all phytopatogen bacteria and fungi in the highest dose. Cytotoxic activity showed an IC50 = 50.5 μg/mL. Antioxidant activity showed an IC50 = 106.6 μg/mL after 45 min experimental time. Based on the presented results, it is possible to conclude that thymol chemotype O. vulgare essential oil could be potentially used as a herbicide with selective effects on monocot plant species.

Uji Ekstrak Bawang Bombay sebagai Anti Bakteri Gram Positif Staphylococcus aureus dengan Metode Difusi Cakram

2012 ◽  
Vol 12 (2) ◽  
pp. 68
Author(s):  
Wuryanti Wuryanti ◽  
Nies Suci Mulyani ◽  
Mukhammad Asy'ari ◽  
Purbowatiningrum Ria Sarjono
Keyword(s):  
Allium Cepa ◽  
Human Life ◽  
Antimicrobial Property ◽  
Antimicrobial Properties ◽  
Living Organism ◽  
Inhibition Zone ◽  
Antimicrobial Test ◽  
Allium Cepa L ◽  
Paper Disc

Microbe is a microscopic living organism which is closely linked to human life. One of pathogenic microorganisms such as Staphylococcus aureu,. may cause diseases. Sulfure of several plants are bioactive as antimicrobial. The properties of sulfure in Allium cepa L leads to an assumption that it has antimicrobial properties. Therefore, this study involved the antimicrobial test. The determination steps of antimicrobial Allium cepa L extract consisted of the Allium cepa L extract preparation, microbial regeneration and antimicrobial test. The antibacterial determination were performed by paper disc method. Based on the research data, determination of antimicrobial Allium cepa L extract resulted in inhibition zone area showed that the extract owned antimicrobial property.

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Exploiting the Potential of Moringa oleifera Oil/Polyvinyl Chloride Polymeric Bionanocomposite Film Enriched with Silver Nanoparticles for Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Musarat Amina ◽  
Nawal M. Al Musayeib ◽  
Nawal A. Alarfaj ◽  
Maha F. El-Tohamy ◽  
Hisham E. Orabi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Polyvinyl Chloride ◽  
Moringa Oleifera ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
X Ray ◽  
Different Strains

The present study focused on the prospect of fabricating a polymeric naturally extracted Moringa oleifera oil bionanocomposite film enriched with silver nanoparticles for antimicrobial activity. In this study, a standard concentration of Moringa oleifera oil (5-10 wt%) was used to fabricate a polymeric bionanocomposite film using polyvinyl chloride (PVC) enriched with silver nanoparticles. The active constituents of the extracted Moringa oleifera oil were verified using gas chromatography-mass spectrometry. Spectroscopic and microscopic techniques, including scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray analysis, were employed to characterize and study the surface morphology of the fabricated bionanocomposite film. The antimicrobial activity of the fabricated bionanocomposite film was investigated using different strains of bacteria and fungus. The results revealed well-oriented and excellently dispersed silver nanoparticles in the PVC-Moringa oleifera oil matrix. The bionanocomposite was able to inhibit the growth of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri, and Candida albicans. The combination of nanoparticles with polymers is opening new routes for engineering fixable composites, which showed antimicrobial properties.

Evidence on antimicrobial properties and mode of action of a chitosan obtained from crustacean exoskeletons on Pseudomonas syringae pv. tomato DC3000

2013 ◽  
Vol 97 (15) ◽  
pp. 6957-6966 ◽  
Author(s):  
A. Y. Mansilla ◽  
L. Albertengo ◽  
M. S. Rodríguez ◽  
A. Debbaudt ◽  
A. Zúñiga ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Mode Of Action ◽  
Antimicrobial Properties ◽  
Tomato Dc3000

ANTIMICROBIAL ACTIVITY OF THE DRUG FUMIOD IN RESPECT TO ISOLATES OF CON-DITIONALLY PATHOGENIC MICRO-FLORA FROM ANIMALS WITH SIGNS OF RESPIRATORY DISEASES.

2020 ◽  
Vol 2 ◽  
pp. 26-30
Author(s):  
V.A. Kuzmin ◽  
◽  
L.S. Vogel ◽  
A.A. Sukhinin ◽  
S.A. Makavchik ◽  
...  
Keyword(s):  
Respiratory Diseases ◽  
Inhibition Zone ◽  
Growth Suppression ◽  
Klebsiella Pneumonia ◽  
Leningrad Region ◽  
Growth Inhibition Zone ◽  
Wide Range ◽  
Glutaraldehyde Solution ◽  
Microbial Growth Inhibition

The purpose of the work is to study the anti-microbial activity of fumigation aerosols of the Fumiyod preparation using bactericidal blocks against isolates of opportunistic mi-croflora from calves and piglets with signs of infectious respiratory diseases. The work was carried out on livestock and pig farms of Volosovsky district of the Leningrad region. Samples of biomaterial (nasal swabs and nasopharyngeal swabs) were taken from dis-eased animals to determine the sensitivity of the microflora to the studied iodine-containing Fumiyod preparation (experiment) and 1% glutaraldehyde solution (control). The biomaterial was plated on elective nutrient media (milk-yolk-salt agar, blood agar, Endo medium, yolk-salt agar). Cultures of microorganisms (Klebsiella pneumonia, Str.pneumoniae, St. aureus, Pro-teus vulgaris, Pseudomonas aeruginosa, E. coli), yeast were isolated from washouts from the nasal cavity and nasopharyngial washings from calves and piglets with symp-toms of bronchopneumonia, pneumonia, bronchitis, rhinitis albicans) and fungi (Mucor sp., Aspergillus fumigatus). Using paper discs, it was found that the Fumiyod preparation in the form of fumigation aero-sols, used in the form of bactericidal check-ers, has a high bactericidal activity in vitro (microbial growth inhibition zone of more than 15 mm) in relation to all microflora isolates from calves and pigs with signs of respiratory diseases, which manifested by a wide range of antimicrobial and antimycotic effects. The final concentration of the Fumiyod preparation was revealed for the active active substance - 0.20 g / m3, in which a wider spectrum of its antimicrobial (growth suppression zone within 26-32 mm) and antimycotic (growth suppression zone within 27-31 mm ) actions com-pared with 1% glutaraldehyde (control), respective-ly, in the range of 20-25 mm and 21-24 mm.

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