scholarly journals PG and Research Department of Zoology, Kongunadu Arts and Science College, Coimbatore

2015 ◽  
Vol 2 (2) ◽  
pp. 139-142
Author(s):  
Lekeshmanaswamy M ◽  
Anusiya devi K ◽  
Manish Kumar B ◽  
Vasuki C.A
Keyword(s):  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Maximum Activity ◽  
Klebsiella Pneumonia ◽  
Moderate Activity ◽  
Candida Sp ◽  
Antibacterial Assay ◽  
Padina Gymnospora ◽  
Science College

In this study Gracilaria edilis, sargassam and Padina gymnospora from Raameshwaram sea, Tamil Nadu were collected, identified and tested against various pathogenic bacteria. In Antibacterial assay Acetone shows the maximum number of activity against the Salmonella typi (32mm) length of inhibtions occurred andAcetone shows the minimum activity against Klebsiella pneumonia (18mm) of the inhibition level. Under the Antifungal assay Acetone shows the maximum activity against the penicillium.sp, (10mm) of the inhibition. Acetone shows the moderate activity against the Aspergillus niger (5mm) of inhibition and the minimumactivity in candida sp,. (8mm) of inhibition zone level.

scholarly journals TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

2019 ◽  
pp. 69-73
Author(s):  
ANNAMALAI MADURAM ◽  
RAJU KAMARAJ
Keyword(s):  
Antibacterial Activity ◽  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Klebsiella Pneumonia ◽  
Human Pathogens ◽  
Human Pathogenic Bacteria

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce. Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin. Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild. Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

Antibacterial Activity of Lime Peel and Lemongrass Extract as Active Ingredients for Spray Hand Sanitizer

SCISCITATIO ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 16-21
Author(s):  
Elza Gitaa Eirene Djima ◽  
Aniek Prasetyaningsih ◽  
Kukuh Madyaningrana
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Skin Irritation ◽  
Inhibition Zone ◽  
Active Ingredients ◽  
Phytochemical Content ◽  
Hand Sanitizer ◽  
Antibacterial Assay ◽  
Lime Peel ◽  
Peel Extract

Hand sanitizers are widely used as an alternative way to maintain hand hygiene from the presence of pathogenic bacteria, such as Staphylococcus aureus. In general, excessive use of alcohol as the main ingredient in hand sanitizers might cause skin irritation. Utilizing antibacterial activity of plants as a component in hand sanitizer could be used as a substitution for alcohol. The aim of this study was to determine the potential antibacterial activity of lime peel and lemongrass extracts as active ingredients for hand sanitizer. Method used in this research included extraction using maceration, qualitative phytochemical test, antibacterial assay, and formulation of spray hand sanitizer and quality test of spray hand sanitizer. In this study alkaloid, flavonoid, saponin, tannin and terpenoid were found as phytochemical content of lime peel, while lemongrass extract contain fla vonoid, tanin, saponin and steroid. Compared to lemongrass extract, lime peel extract with concentration 40%showed the optimum inhibition zone of S. aureus and chosen as active ingredient in spray hand sanitizer formulation. Formulated spray hand sanitizer with lime peel extract was able to inhibit the growth of S. aureus bacteria on hand palm.  

scholarly journals Antibacterial Activity of Floral Petals of Some Angiosperms

2019 ◽  
pp. 1-5 ◽  
Author(s):  
L. Rajanna ◽  
N. Santhosh Kumar ◽  
N. S. Suresha ◽  
S. Lavanya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Assay

The in vitro antibacterial assay was carried out against both Gram positive (B. cerus and S. aureus) and Gram negative (E. coli and K. pneumoniae) bacteria. Floral petals of 20 different species of plants were collected and tested for antibacterial activity. The result showed that the petals were active against both Gram positive and Gram negative. Out of 20 floral petals tested, 19 floral petals exhibited antibacterial activity against selected bacterial strains. The minimal inhibitory zone of floral petal discs against human pathogenic bacteria varies from 2 – 6 mm. Rosa carolina and Ruellia tuberosa showed significance inhibition zone for all the bacterial strains while Lantana camara does not show inhibition zone for any of these pathogenic bacteria.

scholarly journals Antibacterial and Wound Healing Activity of Some Agrimonia eupatoria Extracts.

2013 ◽  
Vol 10 (1) ◽  
pp. 152-160 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Wound Healing ◽  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Resistant Bacteria ◽  
Phytochemical Analysis ◽  
Moderate Activity ◽  
Active Compounds ◽  
Wound Healing Activity

The antibacterial activity of some extracts of A. eupatoria (aqueous and ethanolic) against some pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli ) and their activity on wound healing in rats , also the presence of some active compounds in both extracts were detected . The results showed that the ethanolic extract was more effective on inhibiting tested bacteria than the aqueous extract . P.aeruginosa was the most resistant bacteria, while highest inhibition zone appeared on E.coli (20 mm) .There was a moderate activity against S.aureus with inhibition zone 15 mm. by using ethanolic extract (10 mg/ml) . The phytochemical analysis for detection of active compounds revealed the presence of Carbohydrates, Glycosides and Tannins in both extracts, while some of compounds such as Terpenoids and Phenolic compounds (flavonoids) were detected in the ethanolic but not in the aqueous extracts. Prepared ethanolic extract ointment presented obvious activity on wound healing activity in rats in contrast with fucidin ointment and aqueous extract ointment, hence the wound healing was completed in l0 days by using the ethanolic extract ointment, while it was 12 days and 14 days for the aqueous extract ointment and fucidin ointment respectively, in comparison with the untreated wound which needed more than 16 days for healing completion.

scholarly journals Isolation and characterization of bioactive compounds from Euphorbia cotinifolia

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
B. Jayalakshmi ◽  
K. A. Raveesha ◽  
K. N. Amruthesh
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Bioactive Molecules ◽  
Klebsiella Pneumonia ◽  
Human Pathogenic Bacteria ◽  
Isolation And Characterization ◽  
Study Results

Abstract Background Green plants are found to be an effective reservoir for bioactive molecules and can provide appreciable sources of antimicrobial agents. Antibacterial activity of solvent extracts of Euphorbia cotinifolia leaves was tested by agar cup diffusion and broth microdilution methods against some common human pathogenic bacteria viz., Bacillus cereus, Klebsiella pneumonia, Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella typhi. The methanol extract of Euphorbia cotinifolia was subjected to a silica gel column, leading to the isolation of a bioactive compound 1. The structure of compounds was elucidated by spectroscopic techniques and assessed for their antibacterial activity against several human pathogenic bacteria. Results The inhibition zone ranged against some common human pathogenic bacteria was 15.25–19.50 mm, 13.50–19.25 mm, 12–18.50 mm, 15–20 mm, and 13–19 mm for ECMF1, ECMF2, ECMF3, compounds 1, respectively. The MIC was found to be in the range 91–729 μg/ml for the fractions. The inhibition range was recorded between 12–19 and 10–14 mm for methanol and ethyl acetate extracts, respectively. K. pneumoniae, E. aerogenes, and B. subtilis were highly susceptible to methanol extract with the maximum inhibition zone of 19 mm. The MIC of the compound 1 against human pathogens was 78–833 μg/ml. Conclusion The present study results suggest that tested plant extracts have moderate to potent antibacterial activity due to the occurrence of phenols and flavonoids in the extracts. The defensive property of natural antibacterials is mainly due to the presence of these major groups, vitamins, phenols, flavonoids, and carotenoids. In the present study, biologically active diterpene was isolated and the structures of the new diterpenoids isolated from E. cotinifolia were closely related to an ingenol ester.

Users Opinion about the Library Collection and Services in the Arts and Science Colleges in Coimbatore, Tamil Nadu

2019 ◽  
Vol 9 (2) ◽  
pp. 129-133
Author(s):  
A. Bindhu ◽  
R. Jayabal
Keyword(s):  
Tamil Nadu ◽  
Sampling Method ◽  
Library Collection ◽  
The Arts ◽  
Science College ◽  
Library Collections

The aim of the present study is to assess the user opinion about the library collections and services in the arts and Science College in Coimbatore. The researcher has circulated three hundred questionnaires to selected respondents on the basis of the convent sampling method. Out of the three hundred questionnaires, two hundred and seventy questionnaires were received with the required information. The study found that the majority of the respondents gave a very good opinion about the library collection, services and facilities available in the selected arts and science colleges in Coimbatore.

scholarly journals STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media

2016 ◽  
Vol 11 (2) ◽  
pp. 111
Author(s):  
Alfabetian Harjuno Condro Haditomo ◽  
Angela Mariana Lusiastuti ◽  
Widanarni Widanarni
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Inhibition Zone ◽  
Probiotic Bacteria ◽  
Bacterial Disease ◽  
Second Phase ◽  
Bacillus Firmus ◽  
Culture Methods

ABSTRAK   Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau  bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten  terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar.  Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada  uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik  B. firmus sebanyak 108 cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%.   Kata kunci: Bacillus firmus, probiotik, Aeromonas hydrophila, media budidaya   ABSTRACT  Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers’ health  also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%. Keywords: Bacillus FIRMUS, probiotics, Aeromonas hydrophila, media cultivation

scholarly journals Antibacterial effects of the methanolic extract of Glycine Max (Soybean)

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Seyede Asma Hosseini Chaleshtori ◽  
Mehrdad Ataie Kachoie ◽  
Seyed Mojtaba Hashemi Jazi
Keyword(s):  
Glycine Max ◽  
Infectious Diseases ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Penicillin G ◽  
Antibacterial Effects ◽  
Antimicrobial Effects ◽  
Antibiotic Agents

High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural medicinal plants to eliminate risk of infectious diseases. Glycine max is on the most popular and nutritious foods with high antimicrobial effects. The present research was done to study the antibacterial effects of the methanolic extract of G. max against Gram-negative and positive bacteria. G. max seeds of M7 and M9 varieties were purchased and their methanolic extracts were collected. Diameter of the zone of growth inhibition of extracts were measured for pathogenic bacteria using the CLSI guidelines. Antimicrobial effects of G. max extracts were also compared with several antibiotics. Diameter of the inhibition zone of tested bacteria against different treatments of G. max had a ranges of 5.93±0.18 to 22.61±1.80 mm. M7 variety had a higher antimicrobial effects on tested bacteria (P0.05). The highest diameter of the zone of growth inhibition was seen for L. monocytogenes (22.61±1.80 mm) and S. aureus (19.33±1.56 mm). Antimicrobial effects of the methanolic extract of the G. max was also dose-dependent (P0.05). Diameter of the inhibition zone of tested bacteria against different antibiotic agents had a ranges of 5.94±0.38 to 24.95±2.11 mm. Ciprofloxacin, penicillin G tetracycline, gentamicin and azithromycin antibiotic agents exhibited the highest diameter of zone of the growth inhibition for K. pneumoniae (15.71±1.46 mm), L. monocytogenes (23.94±2.03 mm), L. monocytogenes (20.55±1.95 mm), S. aureus (14.27±1.36 mm) and L. monocytogenes (24.95±2.11 mm), respectively. Formulation of antibiotic agent using the 100 mg/mL concentration of the M7 variety of G. max has been recommended for treatment of the cases of infectious diseases

scholarly journals Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa Wallich) as Antibacterial Agent

2018 ◽  
Vol 10 (3) ◽  
pp. 546-552
Author(s):  
Maria Yasinta Moi ◽  
Endang Kusdiyantini ◽  
Sri Pujiyanto
Keyword(s):  
Antibacterial Agent ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Plant Seed ◽  
E Coli ◽  
Antibiotic Compounds ◽  
Screening Result

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

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