scholarly journals Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Zonghai Sheng ◽  
Yaxi Li ◽  
Dehong Hu ◽  
Tianliang Min ◽  
Duyang Gao ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Nonhuman Primates ◽  
Histological Analysis ◽  
Blood Chemistry ◽  
Intravenous Dose ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Fluorescence probes with aggregation-induced emission (AIE) characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution. However, the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation. Here, we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green (ICG) during a study period of 36 days to demonstrate AIE probes are nontoxic. Furthermore, through bright and nontoxic AIE probes and fluorescence imaging in the second window (NIR-II, 1,000–1,700 nm), we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates, breaking the current limitation of millimeter-deep NIR-II fluorescence imaging. Our important findings, i.e., nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates, may facilitate successful translation of AIE probes in clinical trials.

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

Lysosome-targetable polythiophene nanoparticles for two-photon excitation photodynamic therapy and deep tissue imaging

2017 ◽  
Vol 5 (20) ◽  
pp. 3651-3657 ◽  
Author(s):  
Shaojing Zhao ◽  
Guangle Niu ◽  
Feng Wu ◽  
Li Yan ◽  
Hongyan Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Tissue Imaging ◽  
Two Photon Excitation

Polythiophene nanoparticles with large TPA cross section and high1O2generation quantum yield have been developed for simultaneous lysosome-targetable fluorescence imaging and photodynamic therapy.

A near-infrared I emissive dye: toward the application of saturable absorber and multiphoton fluorescence microscopy in the deep-tissue imaging window

2019 ◽  
Vol 55 (35) ◽  
pp. 5111-5114 ◽  
Author(s):  
Can Ren ◽  
Xiangquan Deng ◽  
Wenbo Hu ◽  
Junzi Li ◽  
Xiaofei Miao ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Saturable Absorber ◽  
Near Infrared ◽  
Tissue Imaging ◽  
Saturable Absorption ◽  
Deep Tissue ◽  
Boron Dipyrromethene ◽  
Deep Tissue Imaging ◽  
Photon Fluorescence

A boron-dipyrromethene (BODIPY) dye emitting in the near-infrared (NIR) I region (723 nm) exhibits strong saturable absorption at 680 nm and excellent three-photon fluorescence imaging in the NIR II (1665 nm) window.

scholarly journals Improvement in Tracing Quantum Dot-Conjugated Nanospheres forIn VivoImaging by Eliminating Food Autofluorescence

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Chul-Kyu Park ◽  
Hoonsung Cho
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Signal To Noise Ratio ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Infrared Range ◽  
Long Term Stability ◽  
Magnetic Nanospheres ◽  
Deep Tissue Imaging

Fluorescence imaging using fluorescent probes has demonstrated long-term stability and brightness suitable forin vivodeep-tissue imaging, but it also allows intense background fluorescence associated with food in the near-infrared (IR) range. We investigated effects of changing rodent diet on food autofluorescence, in the presence of quantum dots-conjugated magnetic nanospheres (QD-MNSs). Replacement of a regular rodent diet with a purified diet has great improvement in removing autofluorescence in the near-infrared range ideal forin vivofluorescence imaging. By feeding a purified diet for eliminating ingredients impairing desirable fluorescence signals in the near-IR range, food autofluorescence was clearly eliminated and fluorescence probes, QD-MNSs, introduced by i.v. injection were effectively traced in a mouse by a distinctive signal-to-noise ratio.

Responsive optical probes for deep-tissue imaging: Photoacoustics and second near-infrared fluorescence

2021 ◽  
Vol 173 ◽  
pp. 141-163
Author(s):  
Fei Ding ◽  
Jing Feng ◽  
Xueli Zhang ◽  
Jielin Sun ◽  
Chunhai Fan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Optical Probes ◽  
Near Infrared Fluorescence ◽  
Deep Tissue Imaging

scholarly journals Slow light for deep tissue imaging with ultrasound modulation

2012 ◽  
Vol 100 (13) ◽  
pp. 131102 ◽  
Author(s):  
Huiliang Zhang ◽  
Mahmood Sabooni ◽  
Lars Rippe ◽  
Chulhong Kim ◽  
Stefan Kröll ◽  
...  
Keyword(s):  
Slow Light ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Ultrasound Modulation

scholarly journals Transgenic mice: beyond the knockout

2011 ◽  
Vol 300 (2) ◽  
pp. F291-F300 ◽  
Author(s):  
R. Lance Miller
Keyword(s):  
Transgenic Mice ◽  
Fluorescent Proteins ◽  
Primary Cultures ◽  
Cell Lineage ◽  
Tissue Imaging ◽  
Cell Type ◽  
Deep Tissue ◽  
Pure Cell ◽  
Deep Tissue Imaging ◽  
Tremendous Impact

Transgenic mice have had a tremendous impact on biomedical research. Most researchers are familiar with transgenic mice that carry Cre recombinase (Cre) and how they are used to create conditional knockouts. However, some researchers are less familiar with many of the other types of transgenic mice and their applications. For example, transgenic mice can be used to study biochemical and molecular pathways in primary cultures and cell suspensions derived from transgenic mice, cell-cell interactions using multiple fluorescent proteins in the same mouse, and the cell cycle in real time and in the whole animal, and they can be used to perform deep tissue imaging in the whole animal, follow cell lineage during development and disease, and isolate large quantities of a pure cell type directly from organs. These novel transgenic mice and their applications provide the means for studying of molecular and biochemical events in the whole animal that was previously limited to cell cultures. In conclusion, transgenic mice are not just for generating knockouts.

Designing Upconversion Nanocrystals Capable of 745 nm Sensitization and 803 nm Emission for Deep-Tissue Imaging

2016 ◽  
Vol 22 (31) ◽  
pp. 10801-10807 ◽  
Author(s):  
Liangliang Liang ◽  
Xiaoji Xie ◽  
Daniel Teh Boon Loong ◽  
Angelo Homayoun All ◽  
Ling Huang ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Upconversion Nanocrystals ◽  
Deep Tissue Imaging
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