COMPARISON OF APICAL MICROLEAKAGE FOLLOWING CANAL OBTURATION WITH SINGLE-CONE AND WARM VERTICAL CONDENSATION TECHNIQUES USING DIFFERENT ROOT CANAL SEALERS

2019 ◽  
pp. 101-105
Author(s):  
Thi Ngoc Giang Huynh ◽  
Anh Chi Phan

Microleakage can result in failure of endodontic treatment. An important characteristic of endodontic sealer is sealing ability. The aim of this experimental study was to compare the apical leakage of teeth obturated with single-cone and warm vertical condensation techniques using three different sealers (Cortisomol, AH Plus, Apexit Plus). Materials and Methods: Sixty extracted human lower premolar teeth were selected and randomly assigned to six groups (n = 10), according to the root canal sealer and technique used for obturation: (A) Single-cone technique with Cortisomol; (B) Single-cone technique with AH Plus; (C) Single-cone technique with Apexit Plus; (D) Warm vertical condensation technique with Cortisomol; (E) Warm vertical condensation technique with AH Plus; (F) Warm vertical condensation technique with Apexit Plus. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. Results: The mean penetration length of dye in Cortisomol, AH Plus and Apexit Plus samples were 0.85, 0.98 and 0.58 mm, respectively. The differences between three groups were not significant (p > 0.05); also, the mean dye penetration in obturating with single-cone and warm vertical condensation techniques using Cortisomol, AH Plus, Apexit Plus was not significantly different. Conclusion: Cortisomol, AH Plus, Apexit Plus were similarly effective in prevention of apical microleakage. Differences in the mean dye penetration between the groups which were obturated with Single-cone and warm vertical condensation were not statistically significant. Key words: Microleakage, single-cone, warm vertical condensation, Cortisomol, AH Plus, Apexit Plus

2006 ◽  
Vol 14 (5) ◽  
pp. 341-345 ◽  
Author(s):  
Fábio Dultra ◽  
Juliana Machado Barroso ◽  
Laise Daniela Carrasco ◽  
Alexandre Capelli ◽  
Danilo M. Zanello Guerisoli ◽  
...  

OBJECTIVES: to compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10), according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon) were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm ± 0.07), Epiphany (0.00 mm ± 0.00) and EndoREZ (0.32 mm ± 0.62) did not differ statistically to each other (p>0.01). EndoFill presented the highest dye penetration mean (0.83 mm ± 0.73) and was statistically different from the other sealers (p<0.01). CONCLUSION: the resin-based root canal sealers presented lesser apical microleakage than the zinc oxide and eugenol based sealer. No statistical differences were observed among resin based sealers.


Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 531 ◽  
Author(s):  
Yemi Kim ◽  
Ban-Suk Kim ◽  
Yong-Min Kim ◽  
Donghee Lee ◽  
Sin-Young Kim

The purpose of this study was to compare the penetration ability of calcium silicate root canal sealers and conventional resin-based sealer using confocal laser scanning microscopy (CLSM). A total of 60 recently extracted single-rooted human premolars were used in this study. The root canals were prepared to a size 40/0.06 taper with ProFile rotary instruments and irrigated with NaOCl and EDTA. After drying all canals, the specimens were randomly divided into three experimental groups (n = 20): Group 1, gutta-percha (GP)/AH Plus with continuous wave compaction; group 2, GP/BioRoot RCS with a single-cone technique; and group 3, GP/Endoseal MTA with a single-cone technique. All experimental samples were sectioned perpendicular to their long axis using a low-speed diamond wheel at the apical, middle, and coronal third levels. The penetration abilities of all samples were evaluated using CLSM. A Kruskal–Wallis analysis and a series of Mann–Whitney U post hoc tests were performed. A higher intensity level was found in the coronal area and a lower intensity level in the apical area in all the experimental groups. The AH Plus group showed higher sum fluorescence intensity in the apical and coronal thirds compared with the BioRoot RCS and Endoseal MTA groups, whereas the BioRoot RCS group showed a higher intensity level in the middle third, similar to the AH Plus group. The maximum sealer penetration depth was low in the apical area and high in the coronal area in the AH Plus and Endoseal MTA groups. In the BioRoot RCS group, maximum sealer penetration was observed in the middle third. In conclusion, there were significant differences in sealer penetration pattern and distance according to the root level and sealer type.


2011 ◽  
Vol 58 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Radmila Arbutina ◽  
Aleksandra Djeri ◽  
Natasa Gajic ◽  
Zorica Trnic ◽  
Natasa Trtic

Introduction. Obturation of the root canal is the final phase of endodontic therapy and it is usually used to assess the quality of endodontic treatment. The objective of this study was to examine the quality of apical sealing of the root canal after application of chemically softened gutta-percha. Material and Methods. Thirty extracted single rooted and multi rooted teeth, divided into two groups according to the material for obturation, were used in the study. After root canal instrumentation using step-back technique, obturation was completed using chemically softened gutta-percha and AH Plus and Apexit paste. The effect of apical sealing was assessed regarding the type of material used for the obturation. After obturation, teeth were coated with two coats of varnish and immersed in 50% solution of silver nitrate for twelve hours, and then longitudinally cut using diamond disc. Linear dye penetration was observed using light microscope and twenty five times magnification. The apical leakage was calculated using the measuring scale, previously mounted on microscope lens. Results. The results showed that the average penetration of silver nitrate in single rooted teeth obturated with the AH Plus paste was 132.8 ?m, while in teeth obturated with Apexit, it was 148.6 ?m. The difference was not statistically significant. Apical leakage in multi rooted teeth obturated with softened gutta-percha and AH Plus paste was 117.3 ?m while in teeth obturated with Apexit, it was 158.5 ?m. The difference was statistically significant. Conclusion. The lowest dye penetration in the apical portion of the canal was observed in multi rooted teeth obturated with AH Plus paste and the highest penetration of silver nitrate was in multi rooted teeth obturated with Apexit paste.


2015 ◽  
Vol 16 (2) ◽  
pp. 96-100 ◽  
Author(s):  
Emre Bayram ◽  
Dilek Dalat ◽  
Melike Bayram

ABSTRACT Aim To evaluate the solubility of five different root canal sealers (AH Plus Jet, RealSeal SE, MTA Fillapex, Tubli-Seal, and Acroseal) in chloroform, eucalyptol and Endosolv-E solvents. Materials and methods Ninety root canal sealer samples were prepared and then divided into three groups for immersion in a solvent for 2, 5 or 10 minutes. The mean values of the root canal sealers’ dissolution in the solvents were obtained by the difference between the preimmersion original weight and the post-immersion weight on a digital analytical scale. Data were statistically analyzed by a Kruskal-Wallis test with a Bonferroni correction. Results Chloroform was a more effective solvent than eucalyptol or Endosolv E for all root canal sealers, except for RealSeal SE, at all time points (p < 0.003). RealSeal SE was the least soluble sealer in all solvents at all time points. Conclusion Chloroform demonstrated a superior ability over other solvents in dissolving canal sealing materials, and eucalyptol was found to be the least effective solvent in this study. Clinical significance This study can help to the clinicians about which solvent should be preferred for solving the filling materials in retreatment cases. How to cite this article Bayram E, Dalat D, Bayram M. Solubility Evaluation of Different Root Canal Sealing Materials. J Contemp Dent Pract 2015;16(2):96-100.


2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Soo-Hyuk Lee ◽  
Soram Oh ◽  
Adel Saeed Al-Ghamdi ◽  
Ayman Omar Mandorah ◽  
Kee-Yeon Kum ◽  
...  

The objective of root canal obturation is to achieve a fluid-tight seal. Recently, GuttaFlow bioseal (GB), a root canal sealer composed of polydimethylsiloxane, gutta-percha particles, and bioactive glass ceramics, has been developed, to enhance the sealing ability of root canal filling material. The objective of this study was to assess the sealing ability of GB using a subnanoliter-scaled fluid-flow measuring device and to compare with that of AH Plus (AH). The fluid flow in root canal-filled teeth using either gutta-percha cone (GP) with AH (GAR; n = 10) or GP with GB (GBR; n = 10) and in GP inserted in AH blocks (GA; n = 10) or GP inserted in GB blocks (GB; n = 10) was measured. In addition, fluid flow in sealer blocks of AH (A; n = 10) and GB (B; n = 10), which served as negative controls, was measured. Root canal-filled teeth using GP without any sealer (GR) acted as positive controls (n = 10). The leakage was obtained by calculating the volume of moved water by time (s), after stabilization of the fluid flow was achieved. Statistical analysis was performed using the Kruskal–Wallis test and Mann–Whitney U-test with Bonferroni correction. A p value less than 0.00238 (0.05/21) was considered significantly different. The mean leakages (nL/s) in the groups are as follows: GAR, 0.0958 ± 0.0543; GBR, 0.0223 ± 0.0246; GA, 0.0644 ± 0.0803; GB, 0.0267 ± 0.0182; A, 0.0055 ± 0.0057; B, 0.0052 ± 0.005; and GR, 0.2892 ± 0.3018. The mean leakage in the GBR group was lower than that in the GAR group (p = 0.001), while the mean leakages in the GA and GB groups were not significantly different. GuttaFlow bioseal can be useful in single-cone obturation technique.


2010 ◽  
Vol 04 (02) ◽  
pp. 101-109 ◽  
Author(s):  
Melek Akman ◽  
Serhan Akman ◽  
Oznur Derinbay ◽  
Sema Belli

Objectives: The purpose of this in vitro study was to evaluate gaps or voids occurring in roots filled with three different sealers.Methods: Thirty extracted human single-rooted teeth were decoronated, instrumented using NiTi rotary instruments, divided into three groups (n=10 per group) and obturated with one of the following: Epiphany with Resilon, MetaSEAL with gutta-percha or AH Plus with gutta-percha using the match-taper single-cone technique. After storage for one week in 100% humidity at 37°C, the teeth were horizontally sectioned (n=10). Photographs were taken from the coronal, median and apical parts of the roots using a stereomicroscope at 10X magnification, and the images were then transferred to a computer. The mathematical method known as the ‘Affine Transformation’ was used for the transformation of pixel coordinates to ground coordinates in the Netcad Software program. The mean areas (μm2) of the gaps between the sealer and root dentin or gutta percha/resilon and the gaps between the sealer and/or voids inside the sealer mass were measured, scored on a 0-3 scale and statistically analyzed with the Kruskal-Wallis test.Results: The mean total area of gaps or voids for each sealer was 4631.80 μm2 for the Epiphany- Resilon, 3826.80 μm2 for the MetaSEAL-gutta-percha and 31334 μm2 for the AH Plus-gutta-percha. The MetaSEAL-gutta-percha group showed more gap or void-free interfaces. No significant differences were found among the sealers in the scores for the gap areas (P<.05), and the MetaSEAL showed similar interfaces with Epiphany.Conclusions: No significant differences in the mean areas of gaps or voids were found among the tested resin-based sealers. (Eur J Dent 2010;4:101-109)


Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2482 ◽  
Author(s):  
Deog-Gyu Seo ◽  
Donghee Lee ◽  
Yong-Min Kim ◽  
Dani Song ◽  
Sin-Young Kim

The purpose of this study was to compare the cytotoxic effects and mineralization activity of three calcium silicate-based root canal sealers to those of a conventional resin-based sealer. Experiments were performed using human dental pulp stem cells grown in a monolayer culture. The root canal sealers tested in this study were EndoSequence BC Sealer (Brasseler), BioRoot RCS (Septodont), Endoseal MTA (Maruchi), and AH Plus (Dentsply DeTrey). Experimental disks 6 mm in diameter and 3 mm in height were made and stored in a 100% humidity chamber at 37 °C for 72 h to achieve setting. The cytotoxicity of various root canal sealers was evaluated using a methyl-thiazoldiphenyl-tetrazolium (MTT) assay. To evaluate cell migration ability, a scratch wound healing method was used, and images of the scratch area were taken using a phase-contrast microscope. Cell morphology was evaluated by a scanning electron microscope after direct exposure for 72 h to each sealer disk. In the cell viability assay, there were no significant differences between the EndoSequence BC, BioRoot RCS, Endoseal MTA, and control groups in any experimental period (p > 0.05). In the cell migration assay, there were no significant differences between the EndoSequence BC, Endoseal MTA, and control groups in any experimental period (p > 0.05). BioRoot RCS exhibited slower cell migration relative to EndoSequence BC and Endoseal MTA for up to 72 h (p < 0.05). Conversely, it showed a similar wound healing percentage at 96 h (p > 0.05). In an evaluation of cell morphology, cells in direct contact with EndoSequence BC, BioRoot RCS, and Endoseal MTA disks showed superior spreading compared to those in contact with the AH Plus disk. In an Alizarin red staining assay, EndoSequence BC, BioRoot RCS, and Endoseal MTA showed a significant increase in mineralized nodule formation compared to the AH Plus group (p < 0.05). In conclusion, all calcium silicate-based root canal sealers tested in this study showed good biological properties and mineralization activity compared to conventional resin-based sealer.


2008 ◽  
Vol 34 (7) ◽  
pp. 822-825 ◽  
Author(s):  
M LEONARDO ◽  
D FLORES ◽  
F DEPAULAESILVA ◽  
R DETOLEDOLEONARDO ◽  
L DASILVA

2008 ◽  
Vol 9 (5) ◽  
pp. 60-67
Author(s):  
Leena Smadi ◽  
Azmi Mahafzah ◽  
Ameen Khraisat

Abstract Aim The purpose of this in vitro study was to analyze the antimicrobial activity of root canal sealers by using the agar diffusion test (ADT). Methods and Materials Three categories of root canal sealers were included in the study: resin-based sealers (4), zinc oxide-based sealers (3), and calcium hydroxide-based sealers (2). The microbial strains used were: S. aureus (2 strains), C. albicans (2 strains), and E. faecalis (1 strain). Statistical analysis was conducted using a one-way analysis of variance (ANOVA). Tests of differences were analyzed using the Tukey's test with a value of p < 0.05 considered statistically significant. Results The antimicrobial activity of root canal sealers was ranked in descending order as follows: Sealite Regular, Cortisemol, Dentalis KEZ, AH26, Sealapex, Acroseal/Topseal, and Endorez/AH plus. Conclusion Root canal sealers showed different inhibitory effects depending on their types and the bacterial strains tested. Root canal sealers containing formaldehyde and eugenol proved to be effective against the microorganisms studied. Clinical Significance The incorporation of antimicrobial components into root canal sealers may become an essential factor in preventing the re-growth of residual bacteria and control of bacteria re-entry into the root canal system. Citation Smadi L, Mahafzah A, Khraisat A. An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers. J Contemp Dent Pract 2008 July; (9)5:060-067.


2005 ◽  
Vol 31 (12) ◽  
pp. 886-890 ◽  
Author(s):  
Hasan Oruçoğlu ◽  
Abdulkadir Sengun ◽  
Nihat Yilmaz

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