scholarly journals Biocompatibility and Mineralization Activity of Three Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Dental Pulp Stem Cells

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2482 ◽  
Author(s):  
Deog-Gyu Seo ◽  
Donghee Lee ◽  
Yong-Min Kim ◽  
Dani Song ◽  
Sin-Young Kim
Keyword(s):  
Cell Migration ◽  
Root Canal ◽  
Calcium Silicate ◽  
Dental Pulp ◽  
Experimental Period ◽  
Control Groups ◽  
Human Dental Pulp ◽  
Ah Plus ◽  
And Control ◽  
Root Canal Sealers

The purpose of this study was to compare the cytotoxic effects and mineralization activity of three calcium silicate-based root canal sealers to those of a conventional resin-based sealer. Experiments were performed using human dental pulp stem cells grown in a monolayer culture. The root canal sealers tested in this study were EndoSequence BC Sealer (Brasseler), BioRoot RCS (Septodont), Endoseal MTA (Maruchi), and AH Plus (Dentsply DeTrey). Experimental disks 6 mm in diameter and 3 mm in height were made and stored in a 100% humidity chamber at 37 °C for 72 h to achieve setting. The cytotoxicity of various root canal sealers was evaluated using a methyl-thiazoldiphenyl-tetrazolium (MTT) assay. To evaluate cell migration ability, a scratch wound healing method was used, and images of the scratch area were taken using a phase-contrast microscope. Cell morphology was evaluated by a scanning electron microscope after direct exposure for 72 h to each sealer disk. In the cell viability assay, there were no significant differences between the EndoSequence BC, BioRoot RCS, Endoseal MTA, and control groups in any experimental period (p > 0.05). In the cell migration assay, there were no significant differences between the EndoSequence BC, Endoseal MTA, and control groups in any experimental period (p > 0.05). BioRoot RCS exhibited slower cell migration relative to EndoSequence BC and Endoseal MTA for up to 72 h (p < 0.05). Conversely, it showed a similar wound healing percentage at 96 h (p > 0.05). In an evaluation of cell morphology, cells in direct contact with EndoSequence BC, BioRoot RCS, and Endoseal MTA disks showed superior spreading compared to those in contact with the AH Plus disk. In an Alizarin red staining assay, EndoSequence BC, BioRoot RCS, and Endoseal MTA showed a significant increase in mineralized nodule formation compared to the AH Plus group (p < 0.05). In conclusion, all calcium silicate-based root canal sealers tested in this study showed good biological properties and mineralization activity compared to conventional resin-based sealer.

An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers

2008 ◽  
Vol 9 (5) ◽  
pp. 60-67
Author(s):  
Leena Smadi ◽  
Azmi Mahafzah ◽  
Ameen Khraisat
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Bacterial Strains ◽  
A Value ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Microbial Strains ◽  
And Control ◽  
Root Canal Sealers

Abstract Aim The purpose of this in vitro study was to analyze the antimicrobial activity of root canal sealers by using the agar diffusion test (ADT). Methods and Materials Three categories of root canal sealers were included in the study: resin-based sealers (4), zinc oxide-based sealers (3), and calcium hydroxide-based sealers (2). The microbial strains used were: S. aureus (2 strains), C. albicans (2 strains), and E. faecalis (1 strain). Statistical analysis was conducted using a one-way analysis of variance (ANOVA). Tests of differences were analyzed using the Tukey's test with a value of p < 0.05 considered statistically significant. Results The antimicrobial activity of root canal sealers was ranked in descending order as follows: Sealite Regular, Cortisemol, Dentalis KEZ, AH26, Sealapex, Acroseal/Topseal, and Endorez/AH plus. Conclusion Root canal sealers showed different inhibitory effects depending on their types and the bacterial strains tested. Root canal sealers containing formaldehyde and eugenol proved to be effective against the microorganisms studied. Clinical Significance The incorporation of antimicrobial components into root canal sealers may become an essential factor in preventing the re-growth of residual bacteria and control of bacteria re-entry into the root canal system. Citation Smadi L, Mahafzah A, Khraisat A. An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers. J Contemp Dent Pract 2008 July; (9)5:060-067.

scholarly journals Właściwości alkalizujące wybranych krzemianowo-wapniowych uszczelniaczy kanałowych. Badanie in vitro

2018 ◽  
Vol 64 (4) ◽  
Author(s):  
Przemysław Reszka ◽  
Łukasz Kucharski ◽  
Adam Klimowicz ◽  
Mariusz Lipski
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Ph Values ◽  
Water Ph ◽  
Ah Plus ◽  
Ph Level ◽  
Root Canal Sealers

ABSTRACTIntroduction: The aim of this in vitro study was to assess the pH values of selected calcium-silicate root canal sealers in comparison with AH Plus resin-based root canal sealer.Materials and methods: Six root canal sealers were included in this study: BioRoot RCS (Septodont, France), GuttaFlow bioseal (Coltėne/Whaledent, Switzerland), MTA Fillapex (Angelus, Brazil), Total Fill BC Sealer (FKG, Switzerland), Well-Root ST (Vericom, Korea) and AH Plus (Dentsply, DeTrey, Germany). Materials samples (n = 6) were placed in dishes and immersed in 10 mL of distilled water (pH = 6.8). After 1, 3, 24, 48, 72, 168, 336 and 504 hrs, water pH was determined with a pH meter calibrated with a solution of known pH. Data were statistically analyzed using Student-t test (p < 0.05).Results: There were differences in the water pH between the materials tested. The highest water pHs were exhibited by Total Fill BC Sealer, Well-Root ST and BioRoot RCS (pH ~12), followed by GuttaFlow bioseal (pH ~10.5) and MTA Fillapex (pH ~9). The lowest pH was shown by AH Plus (pH ~8).Conclusions: All calcium-silicate root canal sealers studied are able to elevate the pH level, which could have biological and antimicrobial effects. The AH Plus root canal sealer has poor alkalizing properties.

scholarly journals Biocompatibility of a new epoxy resin-based root canal sealer in subcutaneous tissue of rat

2015 ◽  
Vol 09 (01) ◽  
pp. 031-035 ◽  
Author(s):  
Neslihan Simsek ◽  
Levent Akinci ◽  
Orhan Gecor ◽  
Hilal Alan ◽  
Fuat Ahmetoglu ◽  
...  
Keyword(s):  
Root Canal ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Tissue Reaction ◽  
Female Rats ◽  
Root Canal Sealer ◽  
Histologic Evaluation ◽  
Ah Plus ◽  
Rat Tissue ◽  
Root Canal Sealers

ABSTRACT Objective: The objective was to evaluate the subcutaneous biocompatibility of two root canal sealers. Materials and Methods: Thirty female rats were distributed into two groups of 15 animals. Each received subcutaneous dorsal implants: Silicone tubes filled with AH Plus or Obtuseal. After the 11th, 14th, and 45th days, tissues were collected for biopsy and fixed and processed for histologic evaluation. Observations of the cellular inflammatory components, such as lymphocytes and macrophages, were made. The data were analyzed using Kruskal–Wallis and Connover tests (P < 0.05). Results: By the 7th day, lymphocyte infiltration increased in both groups (P = 0.0104). However, macrophage infiltration increased only in the AH Plus group (P = 0.0011). By the 14th and 45th days, lymphocyte and macrophage infiltrations were reduced. At the end of the experimental period, no statistically significant differences were found between the AH Plus group and the Obtuseal group (P > 0.05). Conclusion: After 45 days, Obtuseal yielded a satisfactory tissue reaction; it was biocompatible when tested in subcutaneous rat tissue. On the basis of the obtained results, it was concluded that Obtuseal root canal sealer caused a satisfactory tissue reaction as AH plus, because it was biocompatible when tested in the subcutaneous tissue of rats.

scholarly journals Evaluation of the radiopacity of selected calcium-silicate root canal sealers

2019 ◽  
Vol 65 (4) ◽  
Author(s):  
Przemysław Reszka ◽  
Katarzyna Grocholewicz ◽  
Agnieszka Droździk ◽  
Mariusz Lipski
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
International Standards ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Ah Plus ◽  
Root Canal Sealers

Introduction: International standards require adequate radiopacity of root canal filling materials to distinguish them from natural structures, such as tooth tissues, and to allow evaluation of the quality of the root canal filling.The aim of this in vitro study was to assess the radiopacity of selected calcium-silicate root canal sealers compared with the resin-based AH Plus root canal sealer.Materials and methods: The study included 6 root canal sealers: BioRoot RCS, GuttaFlow bioseal, MTA Fillapex, Total Fill BC Sealer, Well-Root ST and AH Plus. Six disc-shaped samples were prepared from all analysed materials (4 mm in diameter and 1 mm thick) and analysed using a digital radiography technique and aluminium step wedges (1.5–15 mm thick) as a standard reference. The radiopacity was determined using computer image analysis. The data was statistically analysed using a Tukey’s range test.Results: All root canal sealers showed radiopacity at least corresponding to the 3 mm aluminium step wedge. AH Plus (13.23 mm Al) showed the highest radiopacity, followed by Total Fill BC Sealer (7.93 mm Al), Well-Root ST (7.12 mm Al), BioRoot RCS (5.93 mm Al) and GuttaFlow bioseal (5.08 mm Al ). MTA Fillapex (3.02 mm Al) showed the lowest radiopacity.Conclusions: Although the evaluated root canal sealers show different radiopacities, all the materials met the criteria laid down by the International Organization of Standardization.

scholarly journals Evaluation of the Biocompatibility of a Recent Bioceramic Root Canal Sealer (BioRoot™ RCS): In-vivo Study

2020 ◽  
Vol 8 (D) ◽  
pp. 100-106
Author(s):  
Laila Hussein El-Mansy ◽  
Magdy Mohamed Ali ◽  
Reham EL Sayed Hassan
Keyword(s):  
Root Canal ◽  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Physical Parameters ◽  
Ah Plus ◽  
Negative Controls ◽  
And Control ◽  
Root Canal Sealers ◽  
Selection Of

BACKGROUND: Recently, new calcium silicate bioceramic sealers were introduced to the market. The selection of root canal sealers should not only be based on the different physical parameters but also on local biocompatibility and tissue tolerance. AIM: This study aimed to evaluate and compare the in-vivo biocompatibility of a BioRoot RCS in parallel to MTA Fillapex and AH Plus sealers. METHODS: Polyethylene tubes containing the freshly mixed test materials were implanted in the subcutaneous tissue of 32 Wistar rats. Empty tubes served as negative controls. After 7, 14, 30, and 60 days, the animals were sacrificed, and the implants with surrounding tissues were processed for routine histological analysis. Histological sections were analyzed under light microscopy. The tissue response was determined by the inflammatory cell infiltration intensity and the fibrous capsule thickness. RESULTS: Results revealed a statistically significant decrease of the inflammation intensity by time within each group for all tested sealers and control. A well-defined thin capsule was observed for all tested sealers at 60 days. CONCLUSION: BioRoot RCS exhibited rapid recovery of inflammation similar to controls. Thus, within the limitations of this study, it can be considered a biocompatible sealer with acceptable tissue tolerance.

scholarly journals Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 3925
Author(s):  
Yemi Kim ◽  
Donghee Lee ◽  
Dani Song ◽  
Hye-Min Kim ◽  
Sin-Young Kim
Keyword(s):  
Stem Cells ◽  
Cell Migration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Nodule Formation ◽  
Alizarin Red ◽  
Pulp Capping ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
And Control

In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials—ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)—on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2–4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.

An in vitro Comparison of the Effect of Three Different Endodontic Medicaments on the Bond Strength of a Resin-based Endodontic Sealer to the Root Canal Dentin

2016 ◽  
Vol 8 (1) ◽  
pp. 28-32
Author(s):  
Vasundhara Shivanna ◽  
Ravi Bhargavi
Keyword(s):  
Bond Strength ◽  
Root Canal ◽  
Control Groups ◽  
Group I ◽  
Ah Plus ◽  
Bond Strengths ◽  
Root Canal Dentin ◽  
And Control ◽  
Push Out

ABSTRACT Objectives The aim of this study was to compare and evaluate the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine (CHX) gel, and propolis when used as intracanal medicaments on the bond strength of a resin-based sealer (AH Plus Jet; Dentsply DeTrey, Konstanz, Germany) to the root dentin. Materials and methods Sixty freshly extracted single-rooted human teeth were utilized for this study and the crowns were decoronated. The root canals were instrumented and randomized into four groups according to the medicament used: Group I: Control; group II: Ca(OH)2; group III: CHX; group IV: Propolis. The specimens were stored for 10 days in 100% humidity at 37°C, and the intracanal dressings were removed by rinsing with 10 mL of 17% ethylenediaminetetraacetic acid followed by 10 mL of 2.5% sodium hypochlorite, then obturated with gutta-percha and AH Plus Jet sealer. A push-out test to measure the bond strength between the root canal dentin and the sealer was done. Two-way analysis of variance and Tukey’s post hoc pairwise tests were used for statistical analysis. Results The use of intracanal medicaments had shown to significantly influence the push-out bond strengths of the resin sealer used (p < 0.05). At coronal and middle thirds, there was no significant difference in bond strengths among all the four groups (p > 0.05). At apical third, the mean bond strength value for propolis group was significantly superior when compared with the other three groups (p < 0.05). The CHX group showed higher bond strength values at apical third compared with Ca(OH)2 and control groups, but it was not significant (p > 0.05). Conclusion Propolis showed superior push-out bond strength than CHX, Ca(OH)2, and control groups at apical third, while no significant differences were observed among all the groups at coronal and middle thirds. How to cite this article Shivanna V, Bhargavi R. An in vitro Comparison of the Effect of Three Different Endodontic Medicaments on the Bond Strength of a Resin-based Endodontic Sealer to the Root Canal Dentin. CODS J Dent 2016;8(1):28-32.

scholarly journals Improved sealing ability promoted by calcium silicate-based root canal sealers

2018 ◽  
Vol 17 ◽  
pp. 1-8
Author(s):  
Tatiana Vieira de Melo ◽  
Marina Carvalho Prado ◽  
Raphael Hirata Junior ◽  
Sandra Rivera Fidel ◽  
Emmanuel João Nogueira Leal da Silva ◽  
...  
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
Rank Test ◽  
Survival Curves ◽  
Sealing Ability ◽  
Significant Difference ◽  
Ah Plus ◽  
Negative Controls ◽  
Root Canal Sealers ◽  
Positive Controls

Aim: This study investigated the sealing ability produced by AH Plus (Dentsply DeTrey, Konstaz, Germany), EndoSequence BC sealer (Brasseler USA, Savannah, GA, USA), GuttaFlow (Coltène/Whaledent, Altstätten, France) and MTA Fillapex (Angelus, Londrina, Brazil). Methdos: A total of forty-six single-root human canines were prepared and randomly divided into four experimental groups (n=10): MTA Fillapex, EndoSequence BC Sealer, AH Plus or GuttaFlow. Teeth with intact crowns served as negative controls (n=3) and teeth filled with only gutta-percha served as positive controls (n=3). Teeth were mounted in a two-chamber apparatus and exposed to Enterococcus faecalis. The number of days over a 60-days period was recorded for the appearance of turbidity in the lower chamber. Kaplan-Meier method was used to estimate the survival curves. The nonparametric log-rank test was used to compare the survival curves using a significance level of 0.05. Results: The results at the end of the observation time were further analyzed by chi-square testing. All positive controls leaked within 24 h, whereas none of the negative controls leaked after 60 days. EndoSequence BC Sealer and MTA Fillapex had significant less bacterial leakage (P<0.05) than the other tested sealers. No significant difference between AH Plus and GuttaFlow was observed (P>0.05). Conclusion: In conclusion, calcium-silicate based root canal sealers promoted improved sealing ability when compared to other endodontic sealers.

Evaluation of the biological and physicochemical properties of calcium silicate‐based and epoxy resin‐based root canal sealers

2021 ◽  
Author(s):  
Gabriela Cardoso Ferreira ◽  
Lucas Siqueira Pinheiro ◽  
Júlia Silveira Nunes ◽  
Roberta Almeida Mendes ◽  
Cláudia Daniela Schuster ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Epoxy Resin ◽  
Root Canal ◽  
Calcium Silicate ◽  
Root Canal Sealers
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