Microbiological examination for dairy purposes. Methods for detection and/or enumeration of specific groups of microorganisms Staphylococcus aureus

1989 ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Microbiological Examination

STAPHYLOCOCCUS AUREUS SENSITIVITY TO ANTIBIOTICS IN CHILDREN WITH ATOPIC DERMATITIS

2017 ◽  
Vol 14 (6) ◽  
pp. 21-25
Author(s):  
E E Varlamov ◽  
E S Fedenko ◽  
M C Treneva ◽  
V R Voronina ◽  
Z V Zaporozhtseva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Antibiotic Resistance ◽  
Secondary Infection ◽  
Skin Scraping ◽  
Microbiological Examination ◽  
Antibiotics Sensitivity

Background. The aim of this study was to assess the antibiotic resistance of S. aureus isolated from the skin of atopic dermatitis (AD) children. Materials and methods. 256 AD children were included in the study in 2014-2016. Microbiological examination of skin scraping and determination of antibiotics sensitivity were performed. Results. Staphylococcus aureus was isolated in 173 (67,6%) of 256 patients. Resistance to erythromycin was established in 64,6 and 74,6%, respectively. Retrospective analyses showed that S. aureus became more sensitive to ampicillin, ceftriaxone, ciprofloxacin but more resistant to erythromycin. Conclusion. Ceftriaxone and cefoxitin but not erythromycin and ampicillin are the drugs of choice for the treatment of atopic dermatitis complicated by secondary infection in children.

PCR RESEARCH AS AN AUXILIARY METHOD FOR DIAGNOSTICS OF PERIPHRESITICAL INFECTION AFTER ENDOSTREDITISION OF JOINTS (CLINICAL CASE)

2020 ◽  
Vol 65 (5) ◽  
pp. 332-336
Author(s):  
N. Yu. Dobrovol’skaya ◽  
N. P. Prishchepa ◽  
E. V. Preobrazhenskaya ◽  
N. N. Pchelova
Keyword(s):  
Staphylococcus Aureus ◽  
Surgical Treatment ◽  
Antibiotic Therapy ◽  
Clinical Case ◽  
Periprosthetic Infection ◽  
Meca Gene ◽  
Resistance Marker ◽  
Two Stage ◽  
Microbiological Examination ◽  
Culture Negative

Periprosthetic infection (PPI) after arthroplasty of large joints is the third (among the main causes of unsatisfactory results of surgical treatment) a serious threat to the health of patients. The «gold standard» for the diagnosis of PPI is the bacteriological examination of samples of periprosthetic tissues and synovial fluid. In 10-30% of cases, it is impossible to isolate microorganisms, which is explained by the difficulty of cultivation and taking antibiotics before sampling. The purpose of study is to demonstrate the diagnostic value of PCR diagnostics for identifying the genetic material of an infectious pathogen of a culture-negative periprosthetic infection. Material of the study is a description of a clinical case of a culture-negative periprosthetic infection that caused a second two-stage revision of the hip joint prosthesis In the first episode of PPI that occurred 3 years after hip replacement, a microbiological examination of the puncture of the trochanteric zone of the operated joint revealed a massive increase in methicillin-resistant Staphylococcus epidermidis (MRSE). A two-stage revision joint replacement was performed. 5 years after the revision, the patient was hospitalized with clinical and radiological signs of PPI, while examining the puncture of the joint revealed characteristic PPI cytosis. Microbiological examination of punctate and intraoperative aspirate at the first stage of the repeated two-stage revision endoprosthesis replacement did not reveal aerobic and anaerobic microorganisms. In PCR studies, the DNA of methicillin-sensitive Staphylococcus aureus (MSSA) was detected in washouts from the removed components of the endoprosthesis; no resistance marker (mecA gene) was found. Given the concomitant oncological disease, this result determined the appointment of pathogenetic antibiotic therapy, the effectiveness of which was confirmed after 8 weeks at the II stage of revision. The PCR study of joint and trochanteric punctures (before surgery), flushing from the removed spacer components (after ultrasound treatment) and intraoperative aspirate from the joint did not reveal Staphylococcus aureus DNA and resistance marker (mecA gene). In some cases of periprosthetic infection, traumatologists and orthopedists deal with culturally negative results of a microbiological study of the patient’s biomaterial and swabs from the components of endoprostheses in the presence of clinical manifestations of PPI, confirmed by laboratory diagnostics and X-ray examination. According to the literature, such clinical situations are observed in 10-30% of cases and are caused by previous antibiotic therapy in the early stages of an infectious complication. After surgical treatment of PPI for the selection of adequate antibiotic therapy, such patients need to at least indirectly determine the type of infection pathogen, which is achieved by the use of additional diagnostic methods, such as a PRC study. In the case described by us, after a course of antibiotic therapy, prescribed according to the results of the first PCR study, the patient’s body does not contain DNA traces of the desired infectious agent. Thus, the repeated PCR not only confirmed the accuracy of the initial diagnosis of the source of infection, but also further illustrated the success of the rehabilitation of the periprosthetic infection using a correctly selected antibacterial drug at the previous stage of the study. The use of the PCR method made it possible to diagnose the pathogen and prescribe adequate antibiotic therapy for culture-negative periprosthetic infection.

scholarly journals Intravitreal Therapy of Endogenous Endophthalmitis due to Urosepsis – A Case Report

2019 ◽  
Vol 75 (5) ◽  
pp. 277-282
Author(s):  
Jela Valášková ◽  
Jana Sitárová ◽  
Vladimír Krásnik
Keyword(s):  
Internal Medicine ◽  
Staphylococcus Aureus ◽  
Visual Acuity ◽  
Case Report ◽  
Antibiotic Treatment ◽  
Hand Movement ◽  
Endogenous Endophthalmitis ◽  
Intravitreal Therapy ◽  
Microbiological Examination ◽  
Antimycotic Agent

This case report describes a case of endogenous endophthalmitis in a 58 year old man upon a background of urosepsis and bronchitis. The patient was hospitalised at a department of internal medicine in another hospital. The day after admittance he was sent to our clinic for a consultation examination due to worsening of vision and pain in the left eye. The condition was diagnosed as endogenous endophthalmitis. Visual acuity at the first examination was hand movement in front of the eye. Intravitreal therapy was commenced with a combination of two antibiotics (ceftazidime 2 mg/ 0.1 ml and vancomycin 1 mg/0.1 ml) and an antimycotic agent (amphotericin B 10 μg/0.1 ml). Over the course of two weeks the patient received this antibiotic treatment intravitreally 3x in total. During the first application of antibiotics, material was sampled from the anterior chamber of the eye for a microbiological examination – the result was negative. The blood culture tested positive for staphylococcus aureus. Intravenous application of antibiotics (gentamicin 240 mg i.v. every 24 hours and amoxicillin with clavulanic acid 1.2 g i.v. every 8 hours) was set for two weeks, and then antibiotic treatment continued (ciprofloxacin 500 mg every 12 hours) and antimycotic therapy (itraconazole 100 mg every 12 hours) orally for 2 months. Four weeks after the beginning of therapy, visual acuity in the left eye was 20/100 and after two months 20/40. The study emphasises the advantage of multi-disciplinary co-operation.

Dairy cow characteristics related to Staphylococcus aureus isolation from quarter samples

1995 ◽  
Vol 62 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Carsten Enevoldsen ◽  
Yrjö T. Gröhn ◽  
Iver Thysen
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Herds ◽  
Test Reaction ◽  
Cross Sectional ◽  
Staph Aureus ◽  
California Mastitis Test ◽  
Microbiological Examination ◽  
Potential Risk Factors ◽  
Sole Ulcers ◽  
Clinical Measures

SummaryA comprehensive description of the health status of 2406 cows in 28 herds was used to study complex interrelationships between Staphylococcus aureus occurrence and potential risk factors at the cow level over a broad spectrum of production conditions. The objective of the study was to characterize those cows shedding Staph, aureus in milk and to provide indications of possible causal relations that should be further explored. The estimates are expected to aid in providing valid and precise evaluations of results from cross sectional microbiological examination of bovine (quarter) milk samples. Staph, aureus were isolated at microbiological examination of quarter milk from 5·2, 13·5, 17·9 and 22·0% of 1552, 490, 296 and 277 cows in lactations 1, 2, 3 and 4–9 respectively. Simultaneous isolation of other mastitis pathogens was not significantly associated with occurrence of Staph, aureus. Of the positive samples, 95% had a positive California mastitis test reaction. Among the clinical measures of udder health, only visibly abnormal milk combined with normal udder tissue was associated with increased occurrence of Staph, aureus, although not consistently. Prior antibiotic treatment for udder disorders was not significantly associated with Staph, aureus isolation. The occurrence of sole ulcers in multiple digits was consistently associated with Staph, aureus in the first lactation. Milk yield was involved in interactions with other pathogens isolated, prior veterinary disease treatment, body weight and season of calving. Strong herd-year effects were revealed. This, combined with the interactions, indicated that some herd-specific factors were major determinants of Staph, aureus occurrence. The study indicated which cow characteristics should be taken into account in epidemiological analyses and causal interpretations of data from cross sectional microbiological examinations of dairy herds.

scholarly journals ANÁLISE DA QUALIDADE MICROBIOLÓGICA DE PASTÉIS FRITOS COMERCIALIZADOS POR LANCHONETES E AMBULANTES NO CENTRO DE ITABUNA, BAHIA

2018 ◽  
Vol 9 (3) ◽  
pp. 49
Author(s):  
Ingrid Silva SANTOS ◽  
Marcio Amorim Tolentino LIMA ◽  
Lucas Ribeiro CARVALHO
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
American Public Health Association ◽  
Microbiological Examination ◽  
Health Association

<p>Considerando-se que o acesso à alimentação segura constitui condição básica para a saúde humana, este trabalho teve como objetivo analisar a qualidade microbiológica dos pastéis fritos com recheios de carne e frango, comercializados em lanchonetes e por ambulantes no centro de Itabuna-BA no período de março a abril de 2015. Foram realizadas contagens totais de microrganismos aeróbios mesófilos, enterobactérias, coliformes totais, coliformes termotolerantes, <em>Escherichia coli</em> e estafilococos coagulase positiva (<em>Staphylococcus aureus</em>) de acordo com os métodos presentes na American Public Health Association (APHA), 4ª Edição do <em>Compendium of Methods for the Microbiological Examination of Foods. </em>Foi observado maior crescimento de microrganismos aeróbios mesófilos nas amostras comercializadas por ambulantes móveis devido ao armazenamento à temperatura ambiente que favoreceu melhores condições à proliferação microbiana. Verificou-se que todas as amostras apresentaram condições sanitárias satisfatórias e estavam de acordo com o padrão legal vigente para coliformes a 45ºC, constituindo o processo de fritura fator responsável por reduzir a contaminação proveniente da confecção dos pastéis.  No entanto, 1 (5%) amostra de pastel de frango apresentou estafilococos coagulase positiva acima do permitido pela ANVISA, na RDC nº 12 de janeiro de 2001, indicando que produto estava em condições sanitárias insatisfatórias e impróprio o para o consumo humano. Portanto, entre os problemas verificados na qualidade microbiológica dos pastéis fritos, a contaminação pós-fritura, devido às práticas higiênicas inadequadas dos manipuladores, foi identificada como o principal fator contribuinte à insalubridade do alimento.</p>

scholarly journals DEKONTAMINASI BAKTERI Escherichia coli DAN Staphylococcus aureus PADA SARANG BURUNG WALET DENGAN PERLAKUAN PEMANASAN (Decontamination of Escherichia coli and Staphylococcus aureus in Edible Bird´s Nest Using Heat Treatment)

2016 ◽  
Vol 10 (2) ◽  
pp. 143-147
Author(s):  
Saimah Saimah ◽  
Mirnawati B. Sudarwanto ◽  
Hadri Latif
Keyword(s):  
Escherichia Coli ◽  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Heating Process ◽  
Heating Effect ◽  
E Coli ◽  
Microbiological Examination ◽  
Heating Treatment ◽  
And Staphylococcus Aureus

This research was aimed to examine the heating effect at 70 C for 3.5 seconds on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) decontamination in edible bird´s nest. This study used 40 clean edible bird´s nest samples. Samples were divided into two groups, first group was contaminated with E. coli and second group was contaminated with S. aureus. Each group was divided into two treatments. The first treatment was directly tested for microbiological examination and the second treatment was heating at temperature 70 C for 3.5 seconds prior to microbiological examination. The results showed that both of bacteria E. coli and S. aureus had been destroyed by heating treatment. Heating process at 70 C for 3.5 seconds was effective for decontamination of both E.coli and S.aureus.

scholarly journals Epidemiology and antibiotic succeptibility profile of methicillin sensitive Staphylococcus aureus among livestock and pet animals

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Muhammad Aamir Naseer ◽  
Amjad Islam Aqib ◽  
Muhammad Shoaib ◽  
Iqra Muzammil ◽  
Zeeshan Ahmad Bhutta ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Zoonotic Pathogen ◽  
Caprine Milk ◽  
Microbiological Examination ◽  
Amoxicillin Clavulanic Acid

Staphylococcus aureus is an important zoonotic pathogen that is responsible for a variety of infectious diseases in humans and animals. The present study was designed to check the prevalence and antimicrobial resistance of MSSA from three different animal origins (bovine, caprine and pet). A total of n= 450 samples (150 each source) were collected from bovine, caprine and pets. Collected samples were subjected to S. aurues identification by microbiological examination and confirmed S. aurues isolates were put to oxacillin disk diffusion test to declare them MSSA. The MSSA confirmed isolates were subjected to various antibiotics for susceptibility profiling using Kirby Baur Disk Diffusion test. The present study found higher prevalence of MSSA from caprine origin (goat 83.33%) as compared to pet (cat 69.33%; dog 65.33%) and bovine origin (buffalo 26.66%; cattle 31.66%). The in-vitro findings of current study revealed oxytetracycline and gentamicin presented 100% efficacy against MSSA of all origins while the vancomycin presented >35%, >40% and > 65% resistance against MSSA isolated from bovine, caprine and pet origin respectively. However, ciprofloxacin was equally effective (50%) against MSSA from buffalo and cattle while >80% efficacy was noted against MSSA from cat and dogs. Linezolid and amoxicillin+ clavulanic acid were 77.78% and 66.67% sensitive to MSSA isolates from caprine milk. The present study found higher prevalence of MSSA from bovine, caprine and pet isolates with diversified pattern of susceptibility of different antibiotics from all sources.

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