scholarly journals Comparative Assessment of Fecal Contamination in Piped-To-Plot Communal Source and Point-Of-Drinking Water

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1139
Author(s):  
Jannatul Ferdous ◽  
Rebeca Sultana ◽  
Ridwan Bin Rashid ◽  
Sabera Saima ◽  
Anowara Begum ◽  
...  
Keyword(s):  
Drinking Water ◽  
Low Income ◽  
Water Samples ◽  
Membrane Filtration ◽  
Fecal Contamination ◽  
Paired Data ◽  
Culture Methods ◽  
E Coli ◽  
Wide Mouth ◽  
Hygiene Education

The aim of this study was to compare the water quality of piped-to-plot source water with point-of-drinking water in the households of a low-income urban area in Bangladesh. A total of 430 low-income households and 78 communal sources connected to these households were selected from the East Arichpur area of Dhaka. The water samples were collected from point-of-drinking vessels (household members’ preferred drinking vessels i.e., a mug, glass, or bottle) in households and from linked sources at six-week intervals between September 2014 and December 2015. Water samples were processed using standard membrane filtration and culture methods to quantify E. coli. Analysis of paired data from source and point-of-drinking water collected on the same day showed that fecal contamination increased from source to point-of-drinking water in the households in 51% (626/1236) of samples. Comparison between bottles vs. other wide-mouth vessels (i.e., glasses, mugs, jugs) showed significantly lower odds (p = 0.000, OR = 0.58, (0.43–0.78)) of fecal contamination compared to other drinking vessels. The findings suggest that recontamination and post-treatment contamination at the point of drinking play a significant role in water contamination in households. Hygiene education efforts in the future should target the promotion of narrow-mouth drinking vessels to reduce contamination.

scholarly journals Multiparametric comparison of chromogenic-based culture methods used to assess the microbiological quality of drinking water and the mFC method combined with a molecular confirmation procedure

2014 ◽  
Vol 13 (1) ◽  
pp. 67-72
Author(s):  
Andrée F. Maheux ◽  
Vanessa Dion-Dupont ◽  
Marc-Antoine Bisson ◽  
Sébastien Bouchard ◽  
Éric Jubinville ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
Ease Of Use ◽  
Contamination Rate ◽  
Well Water ◽  
Culture Methods ◽  
E Coli ◽  
Agar Method

MI agar and Colilert®, as well as mFC agar combined with an Escherichia coli-specific molecular assay (mFC + E. coli rtPCR), were compared in terms of their sensitivity, ease of use, time to result and affordability. The three methods yielded a positive E. coli signal for 11.5, 10.8, and 11.5% of the 968 well water samples tested, respectively. One hundred and thirty-six (136) samples gave blue colonies on mFC agar and required confirmation. E. coli-specific rtPCR showed false-positive results in 23.5% (32/136) of cases. In terms of ease of use, Colilert was the simplest method to use while the MI method provided ease of use comparable to all membrane filtration methods. However, the mFC + E. coli rtPCR assay required highly trained employees for confirmation purposes. In terms of affordability, and considering contamination rate of well water samples tested, the Colilert method and the mFC + E. coli rtPCR assay were at least five times more costly than the MI agar method. Overall, compared with the other two methods tested, the MI agar method offers the most advantages to assess drinking water quality.

scholarly journals Is It Human or Animal? The Origin of Pathogenic E. coli in the Drinking Water of a Low-Income Urban Community in Bangladesh

2021 ◽  
Vol 6 (4) ◽  
pp. 181
Author(s):  
Jannatul Ferdous ◽  
Ridwan Bin Rashid ◽  
Rebeca Sultana ◽  
Sabera Saima ◽  
Musharrat Jahan Prima ◽  
...  
Keyword(s):  
Drinking Water ◽  
Low Income ◽  
Water Contamination ◽  
Urban Community ◽  
Fecal Contamination ◽  
Vital Role ◽  
Source Water ◽  
Drinking Water Contamination ◽  
E Coli ◽  
Polymerase Chain

This study aimed to investigate the origin of diverse pathotypes of E. coli, isolated from communal water sources and from the actual drinking water vessel at the point-of-drinking inside households in a low-income urban community in Arichpur, Dhaka, Bangladesh, using a polymerase chain reaction (PCR). Forty-six percent (57/125, CI 95%: 41−58) of the isolates in the point-of-drinking water and 53% (55/103, CI 95%: 45−64) of the isolates in the source water were diarrheagenic E. coli. Among the pathotypes, enterotoxigenic E. coli (ETEC) was the most common, 81% (46/57) of ETEC was found in the point-of-drinking water and 87% (48/55) was found in the communal source water. Phylogenetic group B1, which is predominant in animals, was the most frequently found isolate in both the point-of-drinking water (50%, 91/181) and in the source (50%, 89/180) water. The phylogenetic subgroup B23, usually of human origin, was more common in the point-of-drinking water (65%, 13/20) than in the source water (35%, 7/20). Our findings suggest that non-human mammals and birds played a vital role in fecal contamination for both the source and point-of-drinking water. Addressing human sanitation without a consideration of fecal contamination from livestock sources will not be enough to prevent drinking-water contamination and thus will persist as a greater contributor to diarrheal pathogens.

scholarly journals Analysis of heavy metals and coliform in samples of drinking water collected from municipal ward offices of western suburbs of Mumbai, India

2014 ◽  
Vol 15 (1&2) ◽  
pp. 99-107
Author(s):  
G.V Zodape
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Water Samples ◽  
Fecal Contamination ◽  
E Coli ◽  
Metallic Sheen ◽  
Differential Medium ◽  
Drinking Water Samples

14 drinking water samples were collected from Vile Parle to Dadar of Suburban’s of (P-South ward offices of B.M.C (Bombay Municipal Corporation) Mumbai in the month from June to December 2012. The samples of drinking-water were analyzed for Cu, Zn, Mn, Fe, As, Cr, Ni, Pb, Cd and Hg. From the results so obtained, the contamination due to heavy metals – Zn (3.115 ppm and 7.816 ppm), Mn (3.115 ppm and 7.426 ppm), Fe (1.124 ppm and 2.872 ppm), As (0.011 ppm to 0.091 ppm), Cr (0.188 ppm and 0.998 ppm ), Pb (1.587 ppm and 4.56 ppm) and Cd (0.011 ppm and 0.051 ppm ) was found to be high whereas the contamination due to Cu (0.012 ppm and 0.313 ppm), Ni (0.126 ppm and 0.774 ppm), were found below the acceptable limits and no Hg was detected in the samples of drinking-water. In the present work, MacConkey Broth was used as a differential medium for detection and enumeration of coliforms from a wide variety water samples. The presence of positive doubtful presumptive test immediately suggests that the water is non potable (i.e., both acid and gas develops in a tube after 48 hours incubation). Confirmation suggests that there is fecal contamination in the water under investigation and hence it is non potable. All the fourteen samples (streaked from positive Ma cConkey broth tubes) were found to be contaminated with E. coli, which was further confirmed by the presence of colonies with green metallic sheen observed under a microscope confirmed that the said samples were contaminated with E. coli - the major indicator of fecal contamination.

scholarly journals Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water

2014 ◽  
Vol 13 (2) ◽  
pp. 340-352 ◽  
Author(s):  
Andrée F. Maheux ◽  
Vanessa Dion-Dupont ◽  
Sébastien Bouchard ◽  
Marc-Antoine Bisson ◽  
Michel G. Bergeron ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Ease Of Use ◽  
Well Water ◽  
Positive Signal ◽  
Culture Methods ◽  
E Coli

The MI agar, Colilert®, Chromocult coliform® agar, and DC with BCIG agar chromogenic culture-based methods used to assess microbiological quality of drinking water were compared in terms of their ubiquity, sensitivity, ease of use, growth of atypical colonies and affordability. For ubiquity, 129 total coliform (representing 76 species) and 19 Escherichia coli strains were tested. Then, 635 1-L well water samples were divided into 100 mL subsamples for testing by all four methods. Test results showed that 70.5, 52.7, 36.4, and 23.3% of the non-E. coli total coliform strains and 94.7, 94.7, 89.5, and 89.5% of the 19 E. coli strains yielded a positive signal with the four methods, respectively. They also yielded a total coliform positive signal for 66.5, 51.7, 64.9, and 55.0% and an E. coli positive signal for 16.1, 14.8, 17.3, and 13.4% of the 635 well water samples tested, respectively. Results showed that Colilert® is the most expensive method tested in terms of reactants, yet it is the easiest to use. Large numbers of atypical colonies were also often observed on Chromocult coliform® and DC with BCIG, thereby challenging the target microorganism count. Thus, the MI agar method seems to be the best option for the assessment of drinking water quality.

Assessment of microbiological quality of drinking water from household tanks in Bermuda

2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
General Information ◽  
Total Coliforms ◽  
E Coli ◽  
Electoral Register ◽  
Highly Correlated

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.

Bacteriological quality of drinking water at point of use and hand hygiene of primary food preparers: implications for household food safety

2016 ◽  
Vol 6 (2) ◽  
pp. 224-230
Author(s):  
S. G. D. N. Lakshmi Reddi ◽  
R. Naveen Kumar ◽  
G. M. SubbaRao ◽  
M. Vishnu Vardhana Rao ◽  
R. V. Sudershan
Keyword(s):  
Drinking Water ◽  
Food Safety ◽  
Hand Hygiene ◽  
Low Income ◽  
Water Samples ◽  
Faecal Coliforms ◽  
E Coli ◽  
Point Of Use ◽  
Drinking Water Samples

Quality of water for consumption and food processing activities is universally accepted as an essential component to ensure food safety at household (HH) level. Along with safe water, hand hygiene is also an important factor for reducing diarrheal illnesses. This was a cross-sectional study conducted in rural and urban HHs to detect hygiene indicators in drinking water samples at point of use (PoU) (n = 150) and their association with the hand hygiene of primary food preparers (n = 150). Overall, 24.7% and 9.3% of drinking water samples (PoU), 48% and 20% of hand rinse samples were contaminated by faecal coliforms and E. coli, respectively. Both drinking water (PoU) and hand rinse samples collected from rural HHs showed higher contamination, followed by those from urban slums and low income HHs. Significant association (p < 0.05) and probable risk with faecal coliforms (OR. 2.5; 95% CI: 1.1–5.4) and E. coli (OR. 14.5; 95% CI: 4.1–50.7) was found between hand rinses and drinking water samples that had bacteriological contamination. These results suggest that there was an extensive cross contamination at HH level. So, targeted education is essential on safe food/water handling practices in HHs to prevent food safety risks.

scholarly journals Drivers of microbiological quality of household drinking water – a case study in rural Ethiopia

2017 ◽  
Vol 16 (2) ◽  
pp. 275-288 ◽  
Author(s):  
Muhammed A. Usman ◽  
Nicolas Gerber ◽  
Evita H. Pangaribowo
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Rural Areas ◽  
Fecal Contamination ◽  
Microbiological Quality ◽  
Water Sources ◽  
Multiple Use ◽  
Water Safety ◽  
E Coli

Abstract This study aims at assessing the determinants of microbiological contamination of household drinking water under multiple-use water systems in rural areas of Ethiopia. For this analysis, a random sample of 454 households was surveyed between February and March 2014, and water samples from community sources and household storage containers were collected and tested for fecal contamination. The number of Escherichia coli (E. coli) colony-forming units per 100 mL water was used as an indicator of fecal contamination. The microbiological tests demonstrated that 58% of household stored water samples and 38% of protected community water sources were contaminated with E. coli. Moreover, most improved water sources often considered to provide safe water showed the presence of E. coli. The result shows that households' stored water collected from unprotected wells/springs had higher levels of E. coli than stored water from alternative sources. Distance to water sources and water collection containers are also strongly associated with stored water quality. To ensure the quality of stored water, the study suggests that there is a need to promote water safety from the point-of-source to point-of-use, with due considerations for the linkages between water and agriculture to advance the Sustainable Development Goal 6 of ensuring access to clean water for everyone.

Effects of improved storage containers on the bacteriological quality of household drinking water in low-income urban communities in Ibadan, Nigeria

2015 ◽  
Vol 16 (2) ◽  
pp. 378-387 ◽  
Author(s):  
Elizabeth O. Oloruntoba ◽  
Toyin F. Babalola ◽  
Oyewale M. Morakinyo ◽  
Adejumo Mumuni
Keyword(s):  
Drinking Water ◽  
Urban Communities ◽  
Low Income ◽  
Water Samples ◽  
Total Coliform ◽  
Household Level ◽  
Significant Difference ◽  
Storage Containers ◽  
Hygiene Education ◽  
Ibadan Nigeria

This study assessed the effectiveness of improved storage containers on household drinking water quality in four low-income urban communities in Ibadan, Nigeria. Three hundred randomly selected respondents were interviewed, while 44 households were selected and randomly assigned to four improved container treatment groups: Covered Buckets with Taps (CBT), Covered Buckets without Taps (CB), Covered Kegs with Taps (CKT) and Covered Kegs without Taps (CK). Water samples from springs, regular storage containers (RSC), and improved containers were analysed for total coliform (TC), total viable bacteria (TVB) and Escherichia coli for 2 weeks. About 96% reported using the same containers for cooking and drinking water, while only 23.3% used a form of water treatment. TC count for RSC and CB exceeded the recommended limit. Only 3 (6.8%) of the samples from RSC contained E. coli. A statistically significant difference was observed between the mean TC counts of samples from the improved containers. Percentage reduction in TC count from RSC, and the improved containers (CB, CBT, CK and CKT) were 25.4%, 37.3%, 45.0%, 56.8% and 53.8% respectively. Bacillus, Staphylococcus, Klebsiella, Proteus and Pseudomonas were isolated from the water samples. CK produced the best result. Hygiene education on use of appropriate storage containers for drinking water is recommended at the household level.

An Improved Membrane Filtration Method for Enumeration of Faecal Coliforms and E. Coli by a Fluorogenic β-Glucuronidase Assay

1993 ◽  
Vol 27 (3-4) ◽  
pp. 267-270 ◽  
Author(s):  
M. T. Augoustinos ◽  
N. A. Grabow ◽  
B. Genthe ◽  
R. Kfir
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Membrane Filtration ◽  
Faecal Coliforms ◽  
Environmental Waters ◽  
Filtration Method ◽  
E Coli ◽  
Wide Range ◽  
Pure Cultures ◽  
Glucuronidase Assay

A fluorogenic β-glucuronidase assay comprising membrane filtration followed by selective enumeration on m-FC agar at 44.5°C and further confirmation using tlie 4-metliylumbelliferyl-β-D-glucuronide (MUG) containing medium was evaluated for the detection of Escherichia coli in water. A total of 200 typical blue and non-typical blue colonies were isolated from sea and fresh water samples using initial selective enumeration on m-FC agar. Pure cultures of the selected colonies were further tested using the MUG assay and identified using the API 20E method. Of the colonies tested which were shown to be positive using the MUG assay 99.4% were Escherichia coli. The results of this study indicate the combination of the m-FC method followed by the MUG assay to be highly efficient for the selection and confirmation of E. coli from a wide range of environmental waters.

scholarly journals Comparison of the ColiPlate™ Kit with Two Common E. coli Enumeration Methods for Water

Water ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 1804
Author(s):  
Cassi J. Gibson ◽  
Abraham K. Maritim ◽  
Jason W. Marion
Keyword(s):  
High Risk ◽  
Natural Water ◽  
Membrane Filtration ◽  
Fecal Contamination ◽  
Reference Method ◽  
World Health ◽  
Risk Levels ◽  
E Coli ◽  
Field Samples ◽  
Very High

Quantitatively assessing fecal indicator bacteria in drinking water from limited resource settings (e.g., disasters, remote areas) can inform public health strategies for reducing waterborne illnesses. This study aimed to compare two common approaches for quantifying Escherichia coli (E. coli) density in natural water versus the ColiPlate™ kit approach. For comparing methods, 41 field samples from natural water sources in Kentucky (USA) were collected. E. coli densities were then determined by (1) membrane filtration in conjunction with modified membrane-thermotolerant E. coli (mTEC) agar, (2) Idexx Quanti-Tray® 2000 with the Colilert® substrate, and (3) the Bluewater Biosciences ColiPlate kit. Significant correlations were observed between E. coli density data for all three methods (p < 0.001). Paired t-test results showed no difference in E. coli densities determined by all the methods (p > 0.05). Upon assigning modified mTEC as the reference method for determining the World Health Organization-assigned “very high-risk” levels of fecal contamination (> 100 E. coli CFU/100 mL), both ColiPlate and Colilert exhibited excellent discrimination for screening very high-risk levels according to the area under the receiver operating characteristic curve (~89%). These data suggest ColiPlate continues to be an effective monitoring tool for quantifying E. coli density and characterizing fecal contamination risks from water.

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